Article
Chemistry, Analytical
Lucia Martin-Saiz, Lorena Mosteiro, Jon D. Solano-Iturri, Yuri Rueda, Javier Martin-Allende, Igone Imaz, Ivan Olano, Begona Ochoa, Olatz Fresnedo, Jose A. Fernandez, Gorka Larrinaga
Summary: The study demonstrates the potential of imaging mass spectrometry in lipidomics to highlight the structural features of the human kidney at a spatial resolution of 10 μm. Specific lipid fingerprints help identify different segments of the nephron and interstitial tissue, laying the foundation for further research on the metabolic basis of diseases affecting the human kidney.
ANALYTICAL CHEMISTRY
(2021)
Article
Engineering, Environmental
Xin Diao, Chengyi Xie, Guangshan Xie, Yuanyuan Song, Yanshan Liang, Ruijin Li, Chuan Dong, Lin Zhu, Jianing Wang, Zongwei Cai
Summary: This study utilized advanced techniques to investigate the spatial distribution of lipids in rat brains after exposure to high levels of PM2.5. The findings suggest a possible association between PM2.5 exposure and neurological damage, potentially mediated by reductions in sulfatides and subsequent neuroinflammation in the corpus callosum region.
ENVIRONMENTAL SCIENCE & TECHNOLOGY LETTERS
(2022)
Article
Chemistry, Analytical
Kayle J. Bender, Yongheng Wang, Chuo Ying Zhai, Zoe Saenz, Aijun Wang, Elizabeth K. Neumann
Summary: This study presents a method for mass spectrometry imaging on undecalcified bones, allowing for the analysis of tissue types and lipidomic profiles in different regions of the spine. It is expected to contribute to understanding the mechanisms underlying bone-related diseases.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Tanja Bien, Sebastian Bessler, Klaus Dreisewerd, Jens Soltwisch
Summary: Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) has the potential to visualize the spatial distribution of biomolecules in cell cultures and tissue sections with high resolution, but sample preparation is crucial. A study found that a robust sample preparation protocol can preserve cell morphology and molecular integrity, contributing to the study of cell-to-cell heterogeneities at a molecular level. Advancements in single-cell MALDI imaging could benefit from these protocols.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Lianlian Ma, Qingrong Xie, Mingyi Du, Yudi Huang, Yingying Chen, Dong Chen, Yizhu Xu, Hanhong Xu, Xinzhou Wu, Zhibin Yin
Summary: This study systematically investigated the optimized sample preparation conditions for insects and model organisms in mass spectrometry imaging (MSI), providing guidelines for MSI analysis and expanding its potential in life sciences and environmental sciences.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Biophysics
Mengyuan Sun, Kevin Hu, Joerg Bewersdorf, Thomas D. Pollard
Summary: PCFPs are widely used in super-resolution microscopy and studies of cellular dynamics, but our understanding of their photophysics is limited, hindering their quantitative application. Through experiments on formaldehyde fixation in fission yeast cells, it was found that fixation affects the photophysical properties of mEos3.2, but light fixation does not destroy the molecules. The study provides guidance on quantitatively comparing conditions for imaging mEos3.2-tagged molecules in yeast cells through a combination of imaging assays and mathematical models.
BIOPHYSICAL JOURNAL
(2021)
Review
Biochemical Research Methods
Michal Alexovic, Jan Sabo, Remi Longuespee
Summary: Proteomics applications in various fields such as life and health sciences, pathology, and pharmacology often require handling small cell and tissue samples, where microproteomics plays a crucial role in addressing specific considerations for processing these samples efficiently.
Article
Multidisciplinary Sciences
Lijing Su, Muhammad Athamna, Ying Wang, Junmei Wang, Marina Freudenberg, Tao Yue, Jianhui Wang, Eva Marie Y. Moresco, Haoming He, Tsaffrir Zor, Bruce Beutler
Summary: Sulfatides can activate mouse immune responses via the TLR4-MD-2 complex, but exhibit antagonistic effects in human cells. Their activity is related to the presence of the sulfate group and the length of the fatty acid chain, and their structure mimics the activation mechanism of molecules like LPS in receptors.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Chemistry, Analytical
Angela I. Lopez-Lorente, Francisco Pena-Pereira, Stig Pedersen-Bjergaard, Vania G. Zuin, Sibel A. Ozkan, Elefteria Psillakis
Summary: The ten principles of GSP aim to establish a roadmap for the development of overall greener analytical methodologies by promoting the use of safe solvents/reagents and renewable materials, reducing waste and energy demand, enabling higher sample throughput, procedure simplification/automation, and improving operator safety.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Review
Chemistry, Analytical
Wojciech Wojnowski, Marek Tobiszewski, Francisco Pena-Pereira, Elefteria Psillakis
Summary: AGREEprep is a metric tool that prioritizes sample preparation, evaluating factors such as solvent, material, and reagent choices, waste generation, and energy consumption to calculate the final assessment score. The assessment procedure utilizes intuitive software to generate an easy-to-read information pictogram showing overall performance and threat structure.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Amir Peyman Soleymani, Marcia Reid, Jasna Jankovic
Summary: This research implements an epoxy-free ultramicrotome technique on beginning-of-life (BOL) and end-of-life (EOL) samples of proton exchange membrane fuel cells (PEMFCs) to observe and analyze the electrode structure in detail. Using transmission electron microscopy (TEM) and electron tomography (TEM), images of the ionomer network, carbon particles' structure, and Pt distribution are obtained, providing insights into their structural changes over time.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Review
Biochemistry & Molecular Biology
Van-An Duong, Hookeun Lee
Summary: Liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based proteomics is a powerful technique for analyzing cellular, tissue, and fluid proteomes. While LC-MS/MS and data analysis techniques have been well-developed, sample preparation remains a challenging and laborious process. In this review, various methods for sample preparation in proteomics, as well as devices and methods for integrating different steps of sample preparation and peptide fractionation, are outlined and discussed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Chemistry, Analytical
Colin F. Poole
Summary: High-performance thin-layer chromatography is a promising technique for high-throughput separations. The sample preparation is crucial to control matrix interferences and improve the detectability of target compounds. Recent trends have shifted towards bioassays and mass spectrometry. Traditional methods are being replaced by automated instrumental approaches for faster and streamlined sample processing. Liquid-liquid extraction is commonly used, although solid-phase extraction is gaining popularity. Planar solid-phase extraction is increasingly used for complex samples. Factors driving current interest include faster and simpler sample workflows, analysis of more complex samples, and the determination of minor contaminants with high concentration factors.
JOURNAL OF SEPARATION SCIENCE
(2023)
Article
Plant Sciences
Andreu Cera, Cristobal Verdugo-Escamilla, Juan A. Marin, Sara Palacio
Summary: Calcium biomineralisation is widely seen in plants, but the process of sample processing can cause spurious crystallisation not observed in unaltered plant tissues. By conducting x-ray diffractometry on fresh intact or sliced leaves, as well as on the same leaves after drying, researchers found that common sample processing practices, such as excessive drying and slicing, led to the formation of Ca-sulphate crystals. This study highlights the importance of avoiding sample processing techniques that alter the water balance of plant tissues in future studies on plant biomineralization.
PHYSIOLOGIA PLANTARUM
(2023)
Article
Chemistry, Analytical
Fujia Wang, Tim Veth, Marije Kuipers, Maarten Altelaar, Kelly E. Stecker
Summary: A successful mass spectrometry-based phosphoproteomics analysis relies on effective sample preparation strategies. The performance of the S-Trap protocol for phosphoproteomics studies is unclear, and the addition of phosphoric acid (PA) in the existing protocol is detrimental to downstream phosphopeptide enrichment. This study systematically evaluates the performance of the S-Trap digestion for proteomics and phosphoproteomics, and demonstrates that an optimized S-Trap approach, with trifluoroacetic acid substituted for PA, is a simple and effective method for phosphoproteomics sample preparation.
ANALYTICAL CHEMISTRY
(2023)
Article
Physics, Atomic, Molecular & Chemical
Daniel D. Vallejo, Joseph L. Corstvet, Facundo M. Fernandez
Summary: Electrospray ionization (ESI) is a popular method for generating ions in mass spectrometry (MS). The use of triboelectric nanogenerators as power sources for ESI shows promise in improving the analytical capabilities. This review highlights the fundamentals of ESI driven by DC power supplies, the contrasting qualities to triboelectric nanogenerator power supplies, and its applications in forensics, metabolomics, and protein structure analysis.
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
(2024)
Article
Physics, Atomic, Molecular & Chemical
Florian Kluibenschedl, Anna Ploner, Christina Meisenbichler, Robert Konrat, Thomas Mueller
Summary: Mass spectrometry imaging (MSI) is a powerful analytical technique for localizing chemicals on surfaces. A new interactive approach, called interactive mass spectrometry imaging (IMSI), has been developed using a pen-like ionization interface connected to a mass spectrometer. This approach allows for real-time tracking of the pen's motion on a surface to obtain chemical data. The IMSI method was successfully tested on inkjet-printed letters and mouse brain tissue samples.
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
(2024)
Article
Physics, Atomic, Molecular & Chemical
Shubhangee S. Gaikwad, Saurabh D. Kalkate, Amruta A. Bankar, Amol S. Bansode
Summary: A stable high-performance thin layer chromatographic technique was developed and validated for estimating the content of Paracetamol and Prochlorperazine. The method was validated according to the International Conference on Harmonization requirements. The study also investigated the degradation products of the drugs.
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
(2024)
Article
Physics, Atomic, Molecular & Chemical
Ioan Marginean
Summary: In this study, a coherent classification of electrospray axial regimes is provided based on non-linear fluid dynamics. This classification includes periodic, transient/chaotic, and stationary regimes, and emphasizes the importance of spray current measurements and electrospray characteristic curves in diagnosing the operation and transitions between these regimes. The effect of the electrospray operating regime on the mass spectrometer signal is also summarized.
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
(2024)
Article
Physics, Atomic, Molecular & Chemical
Le Thao Anh Nguyen, Chris J. Bowen, Laura Burchill, Spencer J. Williams, Richard A. J. O'Hair
Summary: D-Cysteinolic acid (D-CA) is an important metabolite in the biosulfur cycle, while a structural isomer, (R)-3-amino-2-hydroxypropanesulfonate ((R)-AHPS), is less common. Through mass spectrometry and density-functional theory, this study reveals the fragmentation reactions of these two isomers and methods to distinguish them.
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
(2024)
Article
Physics, Atomic, Molecular & Chemical
Frank J. Wensink, Deepak Pradeep, P. B. Armentrout, Joost M. Bakker
Summary: Methane and cyclopropane were reacted with Ru+ ions, and the resulting products were identified and characterized using mass spectrometry, IR action spectroscopy, and density functional theory calculations.
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
(2024)
