Multiphoton Imaging With a Novel Compact Diode-Pumped Ti:Sapphire Oscillator

Multiphoton laser scanning microscopy commonly relies on bulky and expensive femtosecond lasers. We integrated a novel minimal-footprint Ti:sapphire oscillator, pumped by a frequency-doubled distributed Bragg reflector tapered diode laser, into a clinical multiphoton tomograph and evaluated its imaging capability using different biological samples, i.e. cell monolayers, corneal tissue, and human skin. With the novel laser, the realization of very compact Ti: sapphire-based systems for high-quality multiphoton imaging at a significantly size and weight compared to current systems will become possible. (C) 2015 Wiley Periodicals, Inc.