Article
Engineering, Environmental
Wenting Yu, Xiaoxia Su, Moxiao Li, Wanting Wan, Ang Li, Hong Zhou, Feng Xu
Summary: This study investigated the effects of matrix strain on the characteristics and bioactivity of MSC-derived exosomes by culturing PDLSCs in three-dimensional magnetically stretched collagen hydrogels. The results showed that altering the miRNA content in exosomes significantly enhanced their bioactivity, leading to improved repair of bone defects in vivo. This research suggests a novel strategy for promoting tissue regeneration through engineered mechanical microenvironments.
CHEMICAL ENGINEERING JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Benson Koh, Nadiah Sulaiman, Mh Busra Fauzi, Jia Xian Law, Min Hwei Ng, Too Lih Yuan, Abdul Ghani Nur Azurah, Mohd Heikal Mohd Yunus, Ruszymah Bt Hj Idrus, Muhammad Dain Yazid
Summary: This study aimed to explore the potential of xeno-free three-dimensional cultures for mesenchymal stem cell (MSCs) expansion in clinical applications. Human serum and human platelet lysate were found to be suitable alternatives to replace foetal bovine serum in MSCs microcarrier cultures. Low glucose DMEM (LG) + human platelet (HPL) lysate was identified as the best xeno-free culture media, promoting high cell yield and maintaining MSC characteristics. This culture system showed improved immunomodulatory activities, suggesting its potential for future clinical applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Gastroenterology & Hepatology
Yu Saito, Tetsuya Ikemoto, Kazunori Tokuda, Katsuki Miyazaki, Shinichiro Yamada, Satoru Imura, Masato Miyake, Yuji Morine, Seiichi Oyadomari, Mitsuo Shimada
Summary: The study found that the new 3D culture system significantly influenced the morphology and gene expression of HLCs, leading to improved hepatocyte functions. HLC transplantation improved serum liver function in mice, especially in T-Bil levels. These results suggest that the new 3D culture protocol is effective for enhancing hepatocyte functions and may hold promise for clinical cell transplantation to treat metabolic diseases.
JOURNAL OF HEPATO-BILIARY-PANCREATIC SCIENCES
(2021)
Article
Chemistry, Multidisciplinary
Min Han, Hongru Yang, Xiangdong Lu, Yuming Li, Zihao Liu, Feng Li, Zehan Shang, Xiaofeng Wang, Xuze Li, Junliang Li, Hong Liu, Tao Xin
Summary: Exosomes derived from three-dimensional culture of MSCs have shown great potential in spinal cord injury therapy, compared to exosomes from conventional two-dimensional culture. It is urgent to develop a reliable, convenient, and effective exosome delivery method for constant in situ release.
Article
Medicine, Research & Experimental
Maryam Rahbar, Reza Asadpour, Mahmoud Azami, Zohreh Mazaheri, Hossein Hamali
Summary: This study found that culturing SSCs on DTM hydrogel could promote their proliferation, providing sufficient SSCs for successful transplantation in azoospermic mice.
Article
Biochemistry & Molecular Biology
Sang-Hoon Yoon, Mi-Rae Bae, Hyeonwoo La, Hyuk Song, Kwonho Hong, Jeong-Tae Do
Summary: This study proposed an efficient method for deriving NSCs from mouse ESCs through a combination of 3D culture for early neural lineage commitment and 2D NSC derivation. The results demonstrated that ESCs could efficiently differentiate into tripotent NSCs through specification in 3D culture followed by 2D culture.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Ali Salehi, Mohammad A. Mobarhan, Javad Mohammadi, Hosein Shahsavarani, Mohammad Ali Shokrgozar, Atefeh Alipour
Summary: The study investigated the characteristics of decellularized cabbage leaves and their potential for promoting bone differentiation, as demonstrated by enhanced osteogenic differentiation of BM-MSCs on the scaffold. These results suggest that decellularized cabbage leaves have great potential for tissue engineering applications in bone regeneration.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Cell Biology
Yuna Naraoka, Yo Mabuchi, Yosuke Yoneyama, Eriko Grace Suto, Daisuke Hisamatsu, Mami Ikeda, Risa Ito, Tetsuya Nakamura, Takanori Takebe, Chihiro Akazawa
Summary: This study identified CD29+ cells with high colony forming and proliferative capacity, successfully constructing meat buds with the potential to differentiate into muscle and adipose cells. The research provides a feasible approach for in vitro production of meat alternatives by utilizing the characteristics of CD29+ cells from bovine tissue.
Article
Biochemistry & Molecular Biology
Luis Oliveros Anerillas, Paul J. Kingham, Mikko J. Lammi, Mikael Wiberg, Peyman Kelk
Summary: Autologous bone transplantation is a common method for reconstructing large bone defects, but has limitations. This study explores the benefits of culturing bone marrow-derived mesenchymal stem cells (BMSCs) in 3D compared to traditional 2D culture. Findings suggest that type I collagen gels are suitable for 3D osteogenic differentiation of BMSCs, potentially enhancing the production of tissue-engineered constructs for bone defect treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Moyassar Basil Hadi Al-Shaibani
Summary: The secretome of MSCs, known as MSC-CM, contains growth factors and cytokines that control cellular responses. Comparing MSC-CM generated from traditional 2DCC and 3DCC using a polystyrene scaffold, 3DCC showed higher concentrations of biomolecules secreted by MSCs, making it a novel approach for therapeutic applications.
BIOTECHNOLOGY LETTERS
(2022)
Article
Cell & Tissue Engineering
Yuejiao Li, Yankai Dong, Ye Ran, Yanan Zhang, Boyao Wu, Jundong Xie, Yanpei Cao, Miaohua Mo, Sen Li, Hao Deng, Wenbo Hao, Shengyuan Yu, Yaojiong Wu
Summary: The study demonstrates that 3D-cultured MSCs exhibit enhanced repair to ischemic stroke by decreasing microglial activation, potentially through the suppression of DAMPs receptor mincle expression and reduced proinflammatory cytokine levels.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Medicine, Research & Experimental
Yongsheng Jia, Cuicui Zhang, Xiangqian Zheng, Ming Gao
Summary: The study found that co-culturing MSCs with HSCs or EPCs/HSCs upregulated the expression of osteogenesis-related genes, but did not affect the efficiency of osteogenesis.
JOURNAL OF INTERNATIONAL MEDICAL RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Prakash Gangadaran, Eun Jung Oh, Ramya Lakshmi Rajendran, Ji Min Oh, Hyun Mi Kim, Suin Kwak, Ho Yun Chung, Jaetae Lee, Byeong-Cheol Ahn, Chae Moon Hong
Summary: Cultured MSCs exhibit different effects on their secretomes depending on whether they are grown as 2D monolayers or 3D spheroids. The secretomes of 3D-MSCs contain higher levels of proangiogenic factors compared to 2D-MCs. The secretomes of 3D-MSCs promote proliferation, migration, and tube formation of ECs, and show enhanced wound healing in a burn injury mouse model.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Gastroenterology & Hepatology
Miho Tamai, Eijiro Adachi, Masaya Kawase, Yoh-ichi Tagawa
Summary: Liver organoid tissue was successfully generated from portal branch-ligated hepatic lobe using extracellular matrix implantation method. This tissue resembles liver lobules, and the embedded fibroblasts contribute to the reconstruction of hepatic interstitial structure.
WORLD JOURNAL OF GASTROENTEROLOGY
(2022)
Article
Cell & Tissue Engineering
Jiwan Choi, Seoon Kang, Bitnara Kim, Seongjun So, Jongsuk Han, Gyeong-Nam Kim, Mi-Young Lee, Seonae Roh, Ji-Yoon Lee, Soo Jin Oh, Young Hoon Sung, Yeonmi Lee, Sung Hoon Kim, Eunju Kang
Summary: The study established an efficient hepatic differentiation protocol for AM-MSCs, replacing fetal bovine serum with polyvinyl alcohol to improve differentiation efficiency, and demonstrated the potential of AM-HPCs as transplantable therapeutic materials for liver disease treatment.
STEM CELL RESEARCH & THERAPY
(2021)