Article
Agriculture, Dairy & Animal Science
Valentina Meucci, Chiara Orsetti, Micaela Sgorbini, Federica Battaglia, Marta Cresci, Francesca Bonelli
Summary: Mastitis is a significant issue on dairy farms, and using a bovine procalcitonin ELISA kit can assess procalcitonin concentrations in plasma but not milk samples.
Article
Biochemistry & Molecular Biology
Ralf Weiskirchen, Sarah K. Schroeder, Sabine Weiskirchen, Eva Miriam Buhl, Bodo Melnik
Summary: Extracellular vesicles, such as exosomes, are small bilayered biovesicles released by cells, enriched with biologically active components. Milk exosomes play a key role in infant growth and development, while exosomes derived from different cell types have regenerative and immunomodulatory properties. Isolation protocols for milk exosomes allow the purification of highly enriched fractions, expressing typical exosomal protein markers.
Article
Immunology
Bharati Matta, Jenna Battaglia, Betsy J. Barnes
Summary: Neutrophil extracellular traps (NETs) are web-like structures formed by activated neutrophils that contain antimicrobial granular proteins, peptides, and enzymes. They can clear microorganisms and inhibit infections, but dysregulated NETs are associated with autoimmune diseases and COVID-19 severity. This study developed new methodologies, using multiplex ELISA and immunofluorescence, to quantify NETs in patient plasma.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Chemistry, Applied
Rongzhi Wang, Juncheng Wang, Haimei Liu, Yehong Gao, Qiang Zhao, Sumei Ling, Shihua Wang
Summary: The study identified a specific IgG against bovine LF for rapid detection of LF content in cow milk using ic-ELISA and lateral flow immunosensors, without the need for expensive equipment and sample pretreatment.
Article
Biochemical Research Methods
Bruna Andersen Pereira de Jesus, Lina Maria Salazar Echeverri, Maria de Lourdes Borba Magalhaes, Gustavo Felippe da Silva
Summary: This study generated and characterized a polyclonal IgY antibody that specifically recognizes the A1 beta-casein protein present in cow's milk. The sensitivity and specificity of both the commercial and produced anti-A1 antibodies were evaluated, with the commercial IgY anti-A1 showing 100% sensitivity and specificity, and the inhouse produced anti-A1 antibody demonstrating a sensitivity of 95.2% and a specificity of 100%, indicating its potential as a reliable and cost effective tool for detecting A1 beta-casein protein in milk samples.
ANALYTICAL BIOCHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Mina Farzaneh, Abdollah Derakhshandeh, Abd Al-Bar Ahmed Al-Farha, Kiro Petrovski, Farhid Hemmatzadeh
Summary: This study developed an indirect ELISA method to detect Myc. bovis antibodies in milk samples, and compared it with the traditional recombinant protein-based ELISA method. The results showed significant agreement between the phage-based ELISA and recombinant protein-based ELISA. The research suggests that phage-based ELISA can be used as an alternative tool for preliminary screening of Myc. bovis-associated mastitis.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Oncology
Ekaterina Nikitina, Kristina Alikhanyan, Michelle Nessling, Karsten Richter, Sylvia Kaden, Claudia Ernst, Stefan Seitz, Liubov Chuprikova, Lisa Haefele, Karin Gunst, Nuh Rahbari, Emrullah Birgin, Erik Rasbach, Mohammad Rahbari, Alexander Brobeil, Miriam Schenk, Markus Buechler, Ethel-Michele de Villiers, Timo Bund, Harald zur Hausen
Summary: Chronic inflammation caused by bovine milk and meat factors (BMMFs) and specific subsets of macrophages leads to the synthesis of oxygen radicals and the induction of mutations in actively replicating cells and replicating single stranded DNA. Cancers arising from this process, known as indirect carcinogenesis by infectious agents, are characterized by the absence of persistent genes from the agent in premalignant or cancer cells. This study investigates the structural properties of pleomorphic vesicles, which are regularly observed in peritumor tissues of colorectal, lung, and pancreatic cancer and express BMMF Rep. The results suggest a contribution of Rep or Rep isoforms to the formation of these vesicles.
INTERNATIONAL JOURNAL OF CANCER
(2023)
Article
Immunology
Xiaozhi Xi, Guokai Xiao, Guiqi An, Lin Liu, Xiaochun Liu, Peiyu Hao, Jennifer Yiyang Wang, Dandan Song, Wengong Yu, Yuchao Gu
Summary: In this study, three shark single-domain antibodies targeting OGT were obtained, and they showed high affinity to OGT. These antibodies can be used for the detection and intracellular co-localization of OGT, providing a powerful tool for the study of OGT and O-GlcNAcylation.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Zoology
Mudussar Nawaz, Iahtasham Khan, Muhammad Shakeel, Arfan Yousaf, Zahid Naseer, Munibullah, Ali Zohaib, Riaz Hussain, Qudrat Ullah
Summary: The study revealed a higher prevalence of brucellosis in buffaloes compared to goats in Islamabad Capital Territory, with commercial production and old age being important risk factors for disease spread. A history of abortion was significantly associated with positive Brucella tests, while a history of stillbirth showed no significant correlation with anti-Brucella antibody titers.
PAKISTAN JOURNAL OF ZOOLOGY
(2021)
Article
Virology
Paul Kinsella, Michael Moso, Genevieve Martin, Theo Karapangiotidis, Di Karamalakis, Suellen Nicholson, Mitch Batty, Kathy Jackson, Madeleine Marsland, Tilda Thomson, Lakshmi Manoharan, Helen O'brien, N. Deborah Friedman, Katherine Bond, Deborah A. Williamson, Chuan Kok Lim
Summary: This study evaluated the clinical performance of two high-throughput JEV assays in different populations and found that these assays exhibited high sensitivity and concordance in diagnosing acute infection and monitoring vaccination.
JOURNAL OF CLINICAL VIROLOGY
(2023)
Article
Immunology
Antonio Arenas, Carmen Borge, Alfonso Carbonero, Ignacio Garcia-Bocanegra, David Cano-Terriza, Javier Caballero, Antonio Arenas-Montes
Summary: After a year of the SARS-CoV-2 epidemic, there is still no specific effective treatment, so finding pharmacological and immunological mechanisms to control the infection is important. Using cow's milk immune to BCoV as a possible control measure against COVID-19, due to the close phylogenetic relationship between COVID-19 and BCoV viruses, can help with specific immune response.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Food Science & Technology
Abu Hasan Sumon, Farjana Islam, Nayan Chandra Mohanto, Rahanuma Raihanu Kathak, Noyan Hossain Molla, Sohel Rana, Gisela H. Degen, Nurshad Ali
Summary: The study revealed that 78.6% of milk and dairy products in Bangladesh were contaminated with AFM(1) in concentrations ranging from 5.0 to 198.7 ng/L. However, yogurt and milk powder samples had lower levels of AFM(1) below the EU regulatory limit for milk. This highlights the need for further monitoring of aflatoxin contamination in milk and dairy products in Bangladesh.
Article
Dermatology
S. Rossi, D. Castiglia, E. Pisaneschi, A. Diociaiuti, A. Stracuzzi, C. Cesario, R. Mariani, G. Floriddia, G. Zambruno, R. Boldrini, D. Abeni, A. Novelli, R. Alaggio, M. El Hachem
Summary: The study found that transmission electron microscopy (TEM) remains relevant in diagnosing EB, while immunofluorescence mapping (IFM) and genetics are essential and complementary tools in the vast majority of EB cases.
JOURNAL OF THE EUROPEAN ACADEMY OF DERMATOLOGY AND VENEREOLOGY
(2021)
Article
Multidisciplinary Sciences
Mario Costa, Ana Marta Pereira, Silvia Coimbra
Summary: Plant development involves constant adjustments of cell wall composition and structure, with the use of fluorescent immunolocalization offering a high-resolution and specific technique to detect multiple glycan epitopes in the same sample, guiding research and linking glycans to specific functions in plants.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2021)
Article
Dentistry, Oral Surgery & Medicine
Massimo Petruzzi, Alberta Lucchese, Maria Contaldo, Marilina Tampoia, Maria Antonia Frassanito, Dorina Lauritano, Fedora della Vella
Summary: This study demonstrated that anti-desmoglein 3 ELISA has high sensitivity and specificity in the diagnosis of oral pemphigus, making it a preferred test for early detection. Additionally, the titres of anti-desmoglein 3 showed a significant correlation with the severity of the disease.
