Review
Parasitology
Rini Chaturvedi, Jyoti Chhibber-Goel, Ishika Verma, Sreehari Gopinathan, Suhel Parvez, Amit Sharma
Summary: The study collected data on sulfadoxine-pyrimethamine resistance mutations from 2005 to 2019, finding that pyrimethamine-resistant double mutations are widespread in Asia, the Middle East, and the Indian sub-continent, while triple mutations prevail in Africa and South America. Single point mutations in sulfadoxine resistance dominate globally.
INTERNATIONAL JOURNAL FOR PARASITOLOGY
(2021)
Article
Infectious Diseases
Haffsatou Sawadogo, Issiaka Soulama, Adama Zida, Cheikna Zongo, Patindoilba Marcel Sawadogo, Kiswendsida Thierry Guiguemde, Seni Nikiema, Salimata Emilie Badoum, Salam Sawadogo, Aicha Tou, Salif Sombie, Chanolle Tchekounou, Sindie Samuel Serme, Rasmata Ouedraogo-Traore, Tinga Robert Guiguemde, Aly Savadogo
Summary: This study investigated the genetic diversity of Plasmodium falciparum and the mutation prevalence in the dhfr and dhps genes in postpartum infected placentas. The results showed a high genetic diversity in msp1 and msp2, suggesting that mutant genotypes in dhfr and dhps could be potential early warning factors for the increase in sulfadoxine-pyrimethamine resistance.
INFECTION AND DRUG RESISTANCE
(2023)
Article
Multidisciplinary Sciences
David Kwame Dosoo, Jeffrey A. Bailey, Kwaku Poku Asante, Felix Boakye Oppong, Karamoko Niare, Jones Opoku-Mensah, Seth Owusu-Agyei, Brian Greenwood, Daniel Chandramohan
Summary: This study determined the prevalence of molecular markers of sulfadoxine-pyrimethamine (SP) resistance among pregnant women in a high malaria transmission area in Ghana. The findings suggest that SP may still be effective for intermittent preventive treatment during pregnancy (IPTp) in this area.
Article
Parasitology
Rodrigue Roman Dongang Nana, Suzanne Sandrine Beack Bayengue, Martin Luther Koanga Mogtomo, Anne Rosalie Ngono Ngane, Vineeta Singh
Summary: This study investigated the presence of drug-resistant alleles in asymptomatic malaria-infected individuals in Cameroon. The results showed the presence of mutant alleles and various SNPs associated with resistance to sulfadoxine-pyrimethamine. Regular profiling of drug resistance markers in asymptomatic populations is crucial for malaria control and elimination programs.
PARASITOLOGY INTERNATIONAL
(2023)
Article
Infectious Diseases
Casimire Wendlamita Tarama, Harouna Sore, Mafama Siribie, Siaka Debe, Rene Kinda, Adama Ganou, Wendyam Gerard Nonkani, Farida Tiendrebeogo, Winnie Bantango, Kassoum Yira, Aladari Sagnon, Sonia Ilboudo, Esther Yeri Hien, Moussa Wandaogo Guelbeogo, NFale Sagnon, Yves Traore, Didier Menard, Adama Gansane
Summary: This study aimed to assess the proportions of mutations in the genes associated with drug resistance in Plasmodium falciparum isolates in Burkina Faso. The findings suggest that the efficacy of current antimalarial drugs is not significantly affected by highly resistant mutants.
Article
Immunology
Sabyasachi Das, Satyajit Tripathy, Ankita Das, Meenakshi Kumari Sharma, Ayan Nag, Amiya Kumar Hati, Somenath Roy
Summary: This study assessed the genetic variants of sulfadoxine-pyrimethamine resistance and the effectiveness of its treatment in eastern India. It found that despite an 8-year withdrawal, sulfadoxine-pyrimethamine resistance continues to increase.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Microbiology
Francis N. Nkemngo, Lymen W. Raissa, Daniel N. Nguete, Cyrille Ndo, Jerome Fru-Cho, Flobert Njiokou, Samuel Wanji, Charles S. Wondji
Summary: Molecular surveillance of malaria is essential for detecting and tracking parasite resistance to anti-malarial drugs. This study conducted molecular techniques to determine the prevalence and genetic diversity of Plasmodium falciparum and Plasmodium malariae resistance markers in humans and mosquitoes in Cameroon. The results showed high prevalence of malaria and the emergence of drug-resistant alleles. The findings highlight the importance of continued surveillance and intervention strategies to prevent the spread of resistance.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Immunology
He Yan, Jun Feng, Jian-hai Yin, Fang Huang, Xiang-li Kong, Kang-ming Lin, Tao Zhang, Xin-yu Feng, Shui-sen Zhou, Jian-ping Cao, Zhi-gui Xia
Summary: A high prevalence of point mutations in the pfdhfr and pfdhps genes of P. falciparum isolates was detected among Chinese migrant workers returning from Africa. The most common haplotype was the triple mutation IRN in pfdhfr, and the predominant alleles were K540E and S436A in pfdhps. The majority of combined haplotypes carried the CIRNI-SGKAA quadruple allele combination.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Infectious Diseases
Hong Quan, Peng Yu, Kokouvi Kassegne, Hai-Mo Shen, Shen-Bo Chen, Jun-Hu Chen
Summary: In 2013, an epidemic of falciparum malaria involving over 820 persons unexpectedly broke out in Shanglin County, Guangxi Zhuang Autonomous Region, China, after a large number of migrant workers returned from Ghana, where they worked as gold miners. The investigation found that the samples collected from Chinese migrant workers from Ghana showed prevalent but relatively low resistance to sulfadoxine-pyrimethamine (SP). It is necessary to strengthen reasonable drug use and management and continue screening for SP resistance.
TROPICAL MEDICINE AND INFECTIOUS DISEASE
(2023)
Article
Microbiology
Stefano R. Rosillo, Pedro Rafael Dimbu, Ana Luisa M. Candido, Je-Hoon Michael Oh, Carolina Miguel Ferreira, Benjamin Nieto Andrade, Sarah Labuda, Roberta Horth, Julia Kelley, Joana F. M. Morais, Filomeno Fortes, Jose Franco Martins, Eldin Talundzic, Mateusz M. Plucinski
Summary: We sequenced the pfdhfr and pfdhps genes in samples of pregnant women in Angola using SP for malaria prevention. We found high proportions of mutations in these genes associated with SP resistance in all provinces. Some low-frequency mutations were also observed. These findings suggest the presence of SP resistance in Angola.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Multidisciplinary Sciences
Aline Gaelle Bouopda Tuedom, Elangwe Milo Sarah-Matio, Carole Else Eboumbou Moukoko, Brice Lionel Feufack-Donfack, Christelle Ngou Maffo, Albert Ngano Bayibeki, Hermann Parfait Awono-Ambene, Lawrence Ayong, Antoine Berry, Luc Abate, Isabelle Morlais, Sandrine Eveline Nsango
Summary: The study evaluated the prevalence of drug resistance gene mutations in different epidemiological settings in Cameroon. The results showed significant differences in the prevalence of mutations between sites, especially for Pfcrt and Pfmdr1 genes. High levels of mutations related to sulfadoxine-pyrimethamine (SP) resistance were detected, raising concerns about the future efficacy of certain malaria control strategies in Cameroon.
Article
Infectious Diseases
Almahamoudou Mahamar, Kelsey M. Sumner, Brandt Levitt, Betsy Freedman, Aliou Traore, Amadou Barry, Djibrilla Issiaka, Adama B. Dembele, Moussa B. Kanoute, Oumar Attaher, Boubacar N. Diarra, Issaka Sagara, Abdoulaye Djimde, Patrick E. Duffy, Michal Fried, Steve M. Taylor, Alassane Dicko
Summary: This study assessed the long-term impact of seasonal malaria chemoprevention (SMC) on Plasmodium falciparum resistance to sulfadoxine-pyrimethamine (SP) and amodiaquine (AQ) in Mali. The results showed that after 2 and 3 years of SMC implementation, there was an increased frequency of the Pfmdr1 N86Y mutation, but no significant change in the frequencies of Pfdhps 540E, Pfdhps 437G, and Pfcrt K76T. The study also identified for the first time the presence of mutations in the Pfdhps gene at low frequencies, suggesting the need for further surveillance in Mali.
Article
Infectious Diseases
Angela Patricia Guerra, Mario Javier Olivera, Liliana Jazmin Cortes, Stella M. Chenet, Alexandre Macedo de Oliveira, Naomi W. Lucchi
Summary: This study evaluated the presence of gene polymorphisms associated with chloroquine and sulfadoxine-pyrimethamine resistance in Plasmodium falciparum in Quibdo, Colombia. The findings confirm the fixation of resistant alleles for chloroquine and pyrimethamine and suggest that these drugs are not suitable for use in Colombia. However, full resistance to sulfadoxine has not been achieved in the region.
Review
Genetics & Heredity
Alexandra T. Roux, Leah Maharaj, Olukunle Oyegoke, Oluwasegun P. Akoniyon, Matthew Adekunle Adeleke, Rajendra Maharaj, Moses Okpeku
Summary: Malaria is a significant global health concern, particularly in Africa, with drug resistance being a major challenge in controlling the disease. Efforts to track drug resistance and monitor sensitivity are crucial to prevent further spread of resistant parasites and potential reappearance of sensitivity in sub-Saharan Africa.
FRONTIERS IN GENETICS
(2021)
Article
Public, Environmental & Occupational Health
Janet Oyieko, Nathanial K. Copeland, Solomon Otieno, Carolyne Kifude, Stephen Ocholla, Jack Hutter, Hunter Smith, Ashleigh Roberds, Shirley Luckhart, V. Ann Stewart
Summary: Individuals infected with HIV-1 are more likely to have asymptomatic parasitemia, which can potentially contribute to the spread of malaria. HIV treatment can help reduce the prevalence of asymptomatic parasitemia over time.
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
(2023)
Article
Infectious Diseases
Javad Dadgar Pakdel, Sedigheh Zakeri, Abbasali Raz, Navid Dinparast Djadid
Article
Parasitology
Christian Mitri, Emmanuel Bischoff, Karin Eiglmeier, Inge Holm, Constentin Dieme, Emma Brito-Fravallo, Abbasali Raz, Sedigheh Zakeri, Mahdokht I. K. Nejad, Navid D. Djadid, Kenneth D. Vernick, Michelle M. Riehle
PARASITES & VECTORS
(2020)
Article
Parasitology
Saeed Nazeri, Sedigheh Zakeri, Akram Abouie Mehrizi, Soroush Sardari, Navid Dinparast Djadid
EXPERIMENTAL PARASITOLOGY
(2020)
Article
Veterinary Sciences
Hasan Bakhshi, Cecile Beck, Sylvie Lecollinet, Maelle Monier, Laurence Mousson, Sedigheh Zakeri, Abbasali Raz, Kourosh Arzamani, Leila Nourani, Navid Dinparast-Djadid, Anna-Bella Failloux
Summary: The study investigated the seroprevalence of West Nile virus, Usutu virus, and tick-borne encephalitis virus in migrating/resident birds and horses in certain provinces of Iran. Results indicated circulation of these viruses among birds and horses, suggesting the need for sustained surveillance of viral infections in animals and anticipating potential future human infections.
VETERINARY MEDICINE AND SCIENCE
(2021)
Article
Genetics & Heredity
Gerry Tonkin-Hill, Shazia Ruybal-Pesantez, Kathryn E. Tiedje, Virginie Rougeron, Michael F. Duffy, Sedigheh Zakeri, Tepanata Pumpaibool, Pongchai Harnyuttanakorn, OraLee H. Branch, Lastenia Ruiz-Mesia, Thomas S. Rask, Franck Prugnolle, Anthony T. Papenfuss, Yao-ban Chan, Karen P. Day
Summary: The researchers developed a computational approach to explore the evolution of specific DNA sequences of the major VSA gene of the human malaria parasite, Plasmodium falciparum, successfully distinguishing DNA signatures specific to each species and identifying geographic signatures related to the out of Africa origin of P. falciparum.
Article
Microbiology
Hasan Bakhshi, Mehdi Fazlalipour, Javad Dadgar-Pakdel, Sedigheh Zakeri, Abbasali Raz, Anna-Bella Failloux, Navid Dinparast Djadid
Summary: The study found that the polyclonal antibodies of Cx. pipiens mosGCTL-1 exhibited 30% blocking activity, and the infection rate decreased on day 5 post-infection, suggesting the presence of other proteins in Cx. pipiens that could potentially act as cooperative receptors for WNV. Additionally, docking results showed that WNV binds with high affinity to the Culex mosquito lectin receptors.
Review
Parasitology
Sakineh Pirahmadi, Sedigheh Zakeri, Navid D. Djadid, Akram A. Mehrizi
Summary: An efficient malaria vaccine is crucial for malaria eradication, and the use of multiple adjuvants in subunit vaccines may be key. Identifying the most effective combination adjuvants can enhance the efficacy of vaccines against malaria.
INTERNATIONAL JOURNAL FOR PARASITOLOGY
(2021)
Article
Radiology, Nuclear Medicine & Medical Imaging
Mehdi Gholamzadeh Baeis, Abolfazl Mozafari, Fatemeh Movaseghi, Mahdi Yadollahzadeh, Ahmad Sohrabi, Mandana Afsharpad, Mohammad Reza Masjedi
Summary: The study investigated the association between patients' records, CT findings, and epidemiological features with COVID-19 severity. It found that factors like older age, SpO(2) < 90%, coughing, and diabetes were risk factors for severe COVID-19 progression. The crazy-paving pattern on CT scans was significantly associated with disease severity and could predict it accurately, providing valuable information for patient management.
IRANIAN JOURNAL OF RADIOLOGY
(2021)
Article
Genetics & Heredity
Rahmat Solgi, Abbasali Raz, Sedigheh Zakeri, Amir Tavakoli Kareshk, Ahmed Yousef, Ahmad Jarehan, Navid Dinparast Djadid
Summary: This study identified 5 leech species collected from rice fields in Guilan province using morphological characters and DNA barcode analysis, including the first report of Hirudo verbana. The current knowledge on leech fauna in Iran is incomplete, and further comparative studies based on molecular and morphological techniques are needed.
Article
Immunology
Sakineh Pirahmadi, Shima Afzali, Mostafa Zargar, Sedigheh Zakeri, Akram Abouie Mehrizi
Summary: Malaria, a mosquito-borne parasitic disease, remains a global health concern without an effective vaccine; subunit vaccines offer hope in controlling malaria, but face challenges in inducing sufficient immune responses; various approaches can be taken to enhance the efficacy of subunit vaccines against malaria.
MICROBIAL PATHOGENESIS
(2021)
Article
Multidisciplinary Sciences
Leila Nourani, Akram Abouie Mehrizi, Sedigheh Zakeri, Navid Dinparast Djadid
Summary: This study sequenced the pvrbp2b gene of Iranian P. vivax isolates and evaluated the genetic diversity and evolutionary characteristics globally. The study found high genetic polymorphism in pvrbp2b, including polymorphic sites in important B-cell epitopes for vaccine design. These findings contribute to the understanding of genetic variation in Plasmodium vivax and provide insights for vaccine development.
Article
Biochemical Research Methods
Masoumeh Ayoub Meigouni, Akram Abouie Mehrizi, Asghar Fazaeli, Sedigheh Zakeri, Navid Dinparast Djadid
Summary: This study aimed to express and purify the PfGCS1 protein from Plasmodium falciparum in E. coli without large fusion tags. By optimizing the gene and culture conditions, the protein was successfully expressed in different E. coli hosts and media cultures, with the highest yield in E. coli BL21 and E. coli Rosetta hosts in TB medium after 16 hours of induction. The expression of the protein was confirmed by western blotting, and improvements were observed with lower temperature and the addition of glucose and ethanol to the culture media.
PROTEIN EXPRESSION AND PURIFICATION
(2022)
Article
Health Care Sciences & Services
Vahideh Moin-Vaziri, Navid Dinparast Djadid, Helen Hoosh-Deghati, Hoda Atta, Abbas Ali Raz, Seyyed Javad Seyyed-Tabaei, Naseh Maleki-Ravasan, Sedigheh Zakeri
Summary: This study investigated the infection status and biological forms of Anopheles stephensi specimens collected from malarious areas in Afghanistan and Iran. The results showed that there were infections in the specimens from Afghanistan, but no infections were found in the specimens from Iran. This emphasizes the important role of An. stephensi as a malaria vector in Afghanistan.
ETHIOPIAN JOURNAL OF HEALTH SCIENCES
(2022)
Article
Immunology
Akram Abouie Mehrizi, Saeed Bagheri Verjani, Sedigheh Zakeri
Summary: The study demonstrated that Advax combined with poly(I:C) as an adjuvant can induce Th1 immune responses and growth inhibitory antibodies against rPfMSP-1(42).
IRANIAN JOURNAL OF IMMUNOLOGY
(2021)
Article
Public, Environmental & Occupational Health
Azar Tahghighi, Akram Abouie Mehrizi, Sedigheh Zakeri
Summary: The new synthesized compounds showed anti-plasmodial activity against both chloroquine-sensitive and resistant Plasmodium falciparum strains without cytotoxicity towards mammalian cells. Compounds 1b, 2a, and 2b demonstrated comparable anti-malarial activity against both chloroquine-sensitive and resistant strains, making them potential lead structures for new anti-malarial drugs.
ASIAN PACIFIC JOURNAL OF TROPICAL MEDICINE
(2021)