Journal
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
Volume 36, Issue 7, Pages 4323-4331Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.ijhydene.2010.12.128
Keywords
Hydrogenases; Glycerol fermentation; pH; Hydrogen production; Escherichia coli
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Funding
- Ministry of Education and Science of the Republic of Armenia [1012-2008]
- Div Of Chem, Bioeng, Env, & Transp Sys
- Directorate For Engineering [0753702] Funding Source: National Science Foundation
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Hydrogenase (Hyd) activity and H-2 production by Escherichia coli were studied at a low pH. H-2 production at pH 5.5 under glycerol fermentation was shown to be similar to 1.5-fold higher than that at pH 6.5 or above but less than that under glucose fermentation. It was inhibited by N,N'-dicyclohexylcarbodiimide: H-2 production inhibition was increased with decreasing pH and almost maximal inhibition was observed at pH 5.5. The data on H-2 production by single and double mutants with defects in different Hyd-enzymes and in fhlA gene suggest that under glycerol fermentation at a low pH, Hyd-1, Hyd-2 and Hyd-4 were operating in a reversed, non-H-2 producing mode. Moreover, a role of fhlA gene in Hyd-3 and Hyd-4 activity in H-2 production is proposed under glucose fermentation at a low pH. Copyright (C) 2011, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.
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