Article
Chemistry, Physical
Jin-Hu Dou, Maxx Q. Arguilla, Yi Luo, Jian Li, Weizhe Zhang, Lei Sun, Jenna L. Mancuso, Luming Yang, Tianyang Chen, Lucas R. Parent, Grigorii Skorupskii, Nicole J. Libretto, Chenyue Sun, Min Chieh Yang, Phat Vinh Dip, Edward J. Brignole, Jeffrey T. Miller, Jing Kong, Christopher H. Hendon, Junliang Sun, Mircea Dinca
Summary: Researchers have successfully grown single crystals of 2D metal-organic frameworks up to 200 µm in size by balancing in-plane and out-of-plane interactions, revealing anisotropic transport and a clear correlation between conductivity and the nature of the metal cation in the sheets.
Article
Chemistry, Physical
Zonglin Li, Mingxin Xie, Guozheng Nie, Junhui Wang, Lujun Huang
Summary: In this study, we developed an optical biosensor based on an all-dielectric metasurface with broken symmetry. The biosensor exhibited excellent performance, thanks to the high-Q quasi-bound states in the continuum (qBICs) and the efficient field confinement. It has potential applications in extreme biochemical sensing, such as COVID-19 detection at ultralow concentrations.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2023)
Article
Chemistry, Multidisciplinary
Mengyu Gao, Yoonjae Park, Jianbo Jin, Peng-Cheng Chen, Hannah Devyldere, Yao Yang, Chengyu Song, Zhenni Lin, Qiuchen Zhao, Martin Siron, Mary C. Scott, David T. Limmer, Peidong Yang
Summary: Halide perovskite is a unique dynamical system with complex structural and chemical processes, but its intrinsic instability makes real-time investigation challenging. However, our research reveals that atomically thin carbon materials can stabilize ultrathin halide perovskite nanostructures and enable atomic-level visualization of their movement. Protected by carbon shells, these structures maintain their integrity under high electron dose rate and exhibit unusual dynamical behaviors. Our work provides a new method to protect beam-sensitive materials and study structure dynamics of nanomaterials.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Chemistry, Multidisciplinary
Hao-Chin Huang, Kai-Yuan Hsiao, Yu-Han Tseng, Yan-De Chen, Ming-Yen Lu
Summary: In this study, an in situ transmission electron microscopy (TEM) heating system was used to investigate the morphological changes of cubic Ag nanoparticles (NPs) and Ag-based core-shell structures induced by sublimation, as well as the influence of shell coverage on thermal stability. It was found that large Ag NPs (>50 nm) showed a three-stage sublimation-induced morphological change, while small Ag NPs (<30 nm) showed a uniform sublimation. The sublimation process of core-shell NPs started at stage II, and the thermal stability was enhanced by carbon shell passivation.
Article
Chemistry, Physical
Xin Luo, Ruixin Hao, Hao Wang, Wenbo Zhai, Zeyu Wang, Zhijun Ning, Yi Yu
Summary: By utilizing low dose in situ high-resolution transmission electron microscopy imaging, we directly observed the nucleation and growth process of the phase transition from orthorhombic gamma-CsPbBr3 to cubic alpha-CsPbBr3 in nanoplates during heating. The normalization of (PbBr6)(4-) octahedra was observed, and phase transition pathways (edge-to-center growth and island growth) were summarized. Phase fluctuation/recovery occurs during the phase transition process. Additionally, we investigated the thermal degradation caused by high temperature, which was found to occur from the outer edge to the center region. This work provides a microscopic basis for the understanding of phase transition and its application in devices.
JOURNAL OF PHYSICAL CHEMISTRY C
(2023)
Article
Food Science & Technology
Amal S. Albedwawi, Reem Al Sakkaf, Tareq M. Osaili, Ahmed Yusuf, Amin Olaimat, Shao-Quan Liu, Giovanni Palmisano, Nagendra P. Shah, Mutamed M. Ayyash
Summary: This paper investigated the ability of lactic acid bacteria (LAB) and Bifidobacterium to remove acrylamide (AA) and examined the mechanism(s) of the AA removal using various microscopic techniques. The results showed that Bifidobacterium breve and Lactiplantibacillus plantarum exhibited higher acrylamide reduction rates. The study also found that both strains could tolerate low pH levels and remove acrylamide under in vitro gastrointestinal conditions.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Golara Kor, Kifayatullah Mengal, Milos Buric, Pavel Kozak, Hamid Niksirat
Summary: The morphology of antennae in six crayfish species was studied using biometrical and ultrastructural methods. Significant differences were found in antenna length, segment length and width ratios, and the number of segments among the species. The morphology of antennae might reflect adaptation to specific habitats, and a combination of morphological features and biometrical measurements could be used to distinguish different crayfish species.
Article
Materials Science, Multidisciplinary
Oana Cojocaru-Miredin, Arun Devaraj
Summary: In the past decade, the applicability of atom probe tomography (APT) has been extended to a wide range of materials including semiconductors and insulators. However, APT can only provide information about composition, making it challenging to analyze the correlation between composition and other material properties. Therefore, researchers worldwide have been combining APT with other microscopy methods to understand the composition-property interrelationships at the same position of the sample. This article provides an overview of these efforts and presents three examples of opportunities in materials science when using correlative microscopy with APT.
Article
Materials Science, Multidisciplinary
Xiaoyu Liu, Tianzhong Yang, Peilin Lang, Yuan Yao, Xi Shen, Richeng Yu
Summary: Epitaxial CrO2 film was successfully grown on sapphire using CVD method. The interfacial structure and chromium valence distribution were characterized, and the presence of a transition layer was observed.
Review
Chemistry, Multidisciplinary
Haofei Zhao, Yuchen Zhu, Huanyu Ye, Yang He, Hao Li, Yifei Sun, Feng Yang, Rongming Wang
Summary: Nanocrystals play a crucial role in material sciences and industry. Designing nanocrystals with desired structures and properties is a major challenge that requires a deep understanding of atomic-scale dynamics. In situ transmission electron microscopy (TEM), especially environmental TEM, has provided valuable insights into nanocrystals. This comprehensive review highlights the progress in the understanding of nanocrystal dynamics, including nucleation and growth, structure evolution, and dynamics in reaction conditions.
ADVANCED MATERIALS
(2023)
Review
Chemistry, Multidisciplinary
Peter Tieu, Xingxu Yan, Mingjie Xu, Phillip Christopher, Xiaoqing Pan
Summary: The development of single atom catalysts (SACs) with carefully controlled properties has been driven by the need for atom efficient catalysts. Advances in transmission electron microscopy (TEM) techniques, including aberration correction, spectroscopy, and in situ/operando observation, have enabled researchers to study SACs at an atomic scale, providing crucial information about their location, characteristics, and stability.
Article
Chemistry, Multidisciplinary
Garth C. Egan, Edmond Y. Lau, Eric Schwegler
Summary: We directly imaged the laser-induced cavitation of micron and nanoscale bubbles using Movie-Mode Dynamic Transmission Electron Microscopy (MM-DTEM). The collapse of isolated nanobubbles was observed to occur in less than 50 ns, while larger bubbles (approximately 2-3 μm) grew and collapsed in less than 200 ns. Bubbles in close proximity to each other exhibited more complex behavior, with longer lifetimes and a higher likelihood of rebounding after collapse.
Article
Multidisciplinary Sciences
Xuexi Yan, Yixiao Jiang, Qianqian Jin, Tingting Yao, Weizhen Wang, Ang Tao, Chunyang Gao, Xiang Li, Chunlin Chen, Hengqiang Ye, Xiu-Liang Ma
Summary: By combining transmission electron microscopy, first-principles calculations, and cathodoluminescence spectroscopy, we demonstrate unexpected strong interfacial interactions at the incoherent AlN/Al2O3 (0001) interface with a large mismatch, which result in the formation of misfit dislocation networks and stacking faults rarely observed at other incoherent interfaces. The strong interfacial interactions significantly tailor the interfacial atomic structure and electronic properties, leading to a reduced band gap and strong interfacial ultraviolet light emission. These findings suggest the potential of incoherent interfaces in developing novel heterojunction materials and devices.
NATURE COMMUNICATIONS
(2023)
Review
Materials Science, Multidisciplinary
Xi Jiang, Nitash P. Balsara
Summary: Electron microscopy has been essential in characterizing polymers, with recent developments allowing for atomic resolution imaging of polymer crystals and providing a comprehensive understanding of strategies towards high-resolution imaging of radiation-sensitive polymer materials at different length scales.
ACS APPLIED POLYMER MATERIALS
(2021)
Article
Chemistry, Multidisciplinary
Mei Wang, Asher C. Leff, Yue Li, Taylor J. Woehl
Summary: This study successfully demonstrated the in situ controlled synthesis of alloyed AuCu nanocrystals under LP-TEM, while maintaining the molecular structure of metal thiolate complexes. The research revealed that polymer capping ligands not only have known functions during LP-TEM synthesis, but also prevent copper oxidation and facilitate the formation of prenucleation cluster intermediates.
Article
Environmental Sciences
Marek Let, Filip Lozek, Antonin Kouba, Milos Buric, Martin Blaha
Summary: The study found that the decline of native crayfish biodiversity caused by invasive crayfish introductions has been well documented, but the fate of their associated biota has been overlooked. The presence of native European branchiobdellids (annelid worms that are ectosymbionts of crayfish) was monitored in three localities in Czech Republic. The results showed that branchiobdellids were not detected in areas with invasive signal crayfish but without the native noble crayfish, and the number of branchiobdellids on signal crayfish was significantly lower compared to the native noble crayfish.
Article
Biochemistry & Molecular Biology
Kifayatullah Mengal, Golara Kor, Pavel Kozak, Hamid Niksirat
Summary: Crustaceans, especially decapods like shrimp, crabs, and lobsters, are a diverse group of organisms that are commercially and ecologically important. They are affected by various environmental factors that can fluctuate beyond their optimal ranges and influence their immune system and health. Understanding the mechanisms by which stressors affect immune parameters in decapods, particularly the abiotic components of the environment, could help mitigate the harmful effects on their production.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2023)
Article
Biodiversity Conservation
Ismael Soto, Ross N. Cuthbert, Danish A. Ahmed, Antonin Kouba, Sami Domisch, Jaime R. G. Marquez, Ayah Beidas, Giuseppe Amatulli, Jens Kiesel, Longzhu Q. Shen, Margarita Florencio, Herlander Lima, Elizabeta Briski, Florian Altermatt, Gait Archambaud-Suard, Peter Borza, Zoltan Csabai, Thibault Datry, Mathieu Floury, Maxence Forcellini, Jean-Francois Fruget, Patrick Leitner, Marie-Helene Lizee, Anthony Maire, Anthony Ricciardi, Ralf B. Schaefer, Rachel Stubbington, Gea H. Van der Lee, Gabor Varbiro, Ralf C. M. Verdonschot, Peter Haase, Phillip J. Haubrock
Summary: The killer shrimp Dikerogammarus villosus is an invasive alien amphipod that has spread across freshwater rivers and streams in Europe, causing ecological and economic impacts. Our study analyzed 96 European time series and identified trends in the population dynamics of D. villosus, as well as its impact on ecological communities. We found that D. villosus is well-established in European waters and its abundance significantly alters recipient community metrics. However, early detection methods are needed to address the cryptic initial introductions of D. villosus.
DIVERSITY AND DISTRIBUTIONS
(2023)
Article
Zoology
Aiman Imentai, Martin Blaha, Lukas Vesely, Jiri Kristan, Tomas Policar
Summary: This study provides detailed photographs and updated information on the morphology and morphometric analysis of the endangered thick-clawed crayfish. It identifies distinctive morphological characters that differentiate the species from other crayfish and contributes to its conservation and protection.
ZOOSYSTEMATICS AND EVOLUTION
(2023)
Article
Fisheries
Pavel Kozak, Kamile Gonca Erol, Oguz Yasar Uzunmehmetoglu, Michiel Tangerman, Michaela Mojzisova, Remziye Ozkok, Antonin Kouba, Sakir Cinar, Adam Petrusek
Summary: Crayfish plague, caused by Aphanomyces astaci, poses a threat to populations of endangered crayfish and affects fisheries and aquaculture. In Turkey, the narrow-clawed crayfish industry suffered from stock collapses due to accidental introduction of crayfish plague. The establishment of closed culturing systems faces challenges from crayfish plague symptoms development and substantial mortality.
Article
Biodiversity Conservation
Ismael Soto, Ross N. Cuthbert, Anthony Ricciardi, Danish A. Ahmed, Florian Altermatt, Ralf B. Schaefer, Gait Archambaud-Suard, Nuria Bonada, Miguel Canedo-Argueelles, Zoltan Csabai, Thibault Datry, Jaimie T. A. Dick, Mathieu Floury, Marie Anne Eurie Forio, Maxence Forcellini, Jean-Francois Fruget, Peter Goethals, Peter Haase, Emma J. Hudgins, J. Iwan Jones, Antonin Kouba, Patrick Leitner, Marie-Helene Lizee, Anthony Maire, John F. Murphy, Davis Ozolins, Jes Jessen Rasmussen, Astrid Schmidt-Kloiber, Agnija Skuja, Rachel Stubbington, Gea H. van der Lee, Rudy Vannevel, Gabor Varbiro, Ralf C. M. Verdonschot, Peter Wiberg-Larsen, Phillip J. Haubrock, Elizabeta Briski
Summary: Understanding the dynamics and trends of invasive species population is crucial for comprehending the changing global species compositions. The Ponto-Caspian region has been a significant contributor to aquatic invasive species in Europe. Over a span of 52 years, Ponto-Caspian macroinvertebrates in central and western European countries increased from two to 29 species. The invasion rates and richness of Ponto-Caspian invaders also exhibited significant increase over time.
BIOLOGICAL INVASIONS
(2023)
Article
Fisheries
Kifayatullah Mengal, Golara Kor, Antonin Kouba, Pavel Kozak, Hamid Niksirat
Summary: Crustacean hemocytes play important roles in immune functions such as coagulation and phagocytosis. In this study, we used in situ approach and transmission electron microscopy technique to investigate the ultrastructural behavior of hemocytes during coagulation and phagocytosis in marbled crayfish. Our findings showed drastic morphological changes of hemocytes during coagulation, including the transformation of cytoplasmic granules and the involvement of nucleus. We also observed the uptake of necrotic tissues by phagocytic hemocytes and the incorporation of digested contents into cellular components, affecting the cell morphology.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2023)
Article
Fisheries
Golara Kor, Kifayatullah Mengal, Milos Buric, Pavel Kozak, Hamid Niksirat
Summary: Regeneration refers to the regrowing and replacing of injured or lost body parts in crayfish. Immune cells play important roles in nerve regeneration. The study found that granules of semi-granulocytes and granulocytes are the main sources of new organelles in the regenerated nerves of crayfish antennae.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Multidisciplinary Sciences
Golara Kor, Kifayatullah Mengal, Milos Buric, Pavel Kozak, Hamid Niksirat
Summary: The morphology of antennae in six crayfish species was studied using biometrical and ultrastructural methods. Significant differences were found in antenna length, segment length and width ratios, and the number of segments among the species. The morphology of antennae might reflect adaptation to specific habitats, and a combination of morphological features and biometrical measurements could be used to distinguish different crayfish species.
Article
Fisheries
Kifayatullah Mengal, Golara Kor, Pavel Kozak, Hamid Niksirat
Summary: Heat shock proteins (HSPs) are essential for proper protein folding and protect aquatic organisms against environmental stressors. They not only serve as molecular chaperones but also activate innate immune responses. Utilizing non-lethal heat shock and exogenous HSP-inducing compounds can enhance protective immunity and reduce stressful conditions in farmed decapod crustaceans.
AQUACULTURE REPORTS
(2023)
Article
Biodiversity Conservation
Phillip J. Haubrock, Ross N. Cuthbert, Paride Balzani, Elizabeta Briski, Carlos Cano-Barbacil, Vanessa De Santis, Emma J. Hudgins, Antonin Kouba, Rafael L. Macedo, Melina Kourantidou, David Renault, Axel E. Rico-Sanchez, Ismael Soto, Mathieu Toutain, Elena Tricarico, Ali Serhan Tarkan
Summary: Biological invasions pose a growing threat to ecosystems, biodiversity, and socio-economic interests. However, there are variations in classifications and definitions of invasive species among European Union member states, leading to differences in the number and proportion of reported invasive species. Using Germany as a case study, this research highlights the gaps in invasive species classifications and suggests that the lower number of reported invasive species in Germany may be attributed to differences in classifications, variations in research effort and focus, and diverse national priorities. This underreporting may be a result of a focus on economic growth, which may have taken precedence over addressing the potential ecological and economic impacts of invasive species.
BIOLOGICAL INVASIONS
(2023)
Review
Entomology
Anna K. Kulessa, Paride Balzani, Ismael Soto, Antonin Kouba, David Renault, Ali Serhan Tarkan, Phillip J. Haubrock
Summary: There is a lack of research and data on the presence, impacts, and management implications of potentially invasive Orthoptera species in Europe, hindering the development of effective management strategies. To address this gap, there is a need for increased efforts to collect and curate data on non-native and possibly invasive Orthoptera in Europe. These efforts will enhance our understanding of their invasion dynamics and facilitate conservation prioritization and effective management policies.
Article
Biology
Boris Liptak, Katarina Zoric, Jiri Patoka, Antonin Kouba, Momir Paunovic
Summary: The aquarium pet trade is a source of potentially invasive crayfish species that can be introduced into new environments, posing a threat to native biodiversity and freshwater ecosystem functioning. Europe, particularly Serbia, is experiencing an increase in invasive crayfish species, such as the marbled crayfish and the red swamp crayfish, traded as ornamentals. The presence of these species in the pet trade could lead to their occurrence in the wild.
Article
Biology
Jiri Patoka, Alexandr Prokin, Cu Nguyen Dinh, Alexander Tsvetkov, Surya Gentha Akmal, Miloslav Petrtyl, Martin Blaha
Summary: Xenophthalmus pinnotheroides is a small subtidal marine crab found in the West Pacific and Northern Indian Oceans. Its native range is poorly documented, but anthropogenic activities pose a significant threat to its habitats. This study provides the first record of the species in Vietnamese waters and predicts its native range using climate matching analysis. The findings highlight potential areas of occurrence and suggest the need for further field surveys for confirmation. These findings also show the range extension of X. pinnotheroides in Asia.
Article
Microscopy
Shengli Zhang, Ying Wang, Bowei Wang, Yi Zeng, Jiani Li, Xingyue Wang, Cuihua Hu, Zhankun Weng, Zuobin Wang
Summary: Curcumin was found to have effects on the activity, migration, and mitochondrial membrane potential of malignant hepatocytes and their mitochondria. The findings from both optical microscopy and atomic force microscopy studies confirm these effects on cell morphology and biomechanics.
Article
Microscopy
Rutika Sansaria, Krishanu Dey Das, Alwin Poulose
Summary: This paper presents a method for quantifying Golgi dispersion images and classifying them using machine learning models. By applying automated image processing and segmentation, extracting features, and using different classifiers, accurate classification of Golgi images is achieved.
Article
Microscopy
Marjorie da Silva, Cintia Eleonora Lopes Justino, Fernando Henrique Carnimeo, Fernando Barbosa Noll, Johan Billen
Summary: This article describes the secretory glands in females of two species of Myzinum and discusses their homology with other glands in the Hymenoptera order. The study finds that the three glands, instead of the commonly believed two, are present in Myzinum species. However, the limited research on the structure, function, and evolution of female accessory glands in Hymenoptera prevents conclusive statements.
Article
Microscopy
Ping Hao, Wen Yin, Xi Chen, Shuangshuang Qin, Yue Yu, Yuan Yuan, Xiaoyu Quan, Bing Hu, Shouhai Chen, Yi Wu
Summary: This study confirmed the secretion of nano-scale exosomes-multivesicular bodies and the interaction of high endothelial cells (HECs) with lysosomes and mitochondria in the small intestine of laying birds. Immunohistochemistry and immunofluorescence results showed the strong immunoreactivity of exosome-related proteins and autophagic-related proteins in the lamina propria of the small intestine. These findings reveal an innovative communication method among HECs in the small intestine.
Article
Microscopy
Stefan Talu, Robert S. Matos, Henrique Duarte da Fonseca Filho, Daniela Predoi, Simona Liliana Iconaru, Carmen Steluta Ciobanu, Liliana Ghegoiu
Summary: This study developed composite layers based on magnesium-doped hydroxyapatite and chitosan, and demonstrated their good biocompatibility and antifungal effects against osteosarcoma cells. The attachment and growth of the cells on the composite layers were promoted, while the growth of Candida albicans was inhibited. The morphological analysis revealed complex 3D patterns on the cancer cell surfaces on the magnesium-containing composite layers.
