A regenerable piezoelectric immunosensor on the basis of electropolymerized polypyrrole for highly selective detection of Staphylococcal Enterotoxin A in foodstuffs

Piezoelectric immunosensors have been developed for the determination of trace concentrations of Staphylococcal enterotoxin A (SEA) in foodstuff. Antibodies against SEA were covalently immobilized on an electropolymerized polypyrrole substrate on a gold electrode which warrants increased stability of the biolayer and sensitive detection. The calibration plot is linear in the 1-80 nga (TM) mLaEuro3/4(1) SEA concentration range, the limit of detection is 0.4 nga (TM) mLaEuro3/4(1), and the time of analysis is < 30 min. The sensor was successfully applied to quantify SEA in chicken meat and milk.