Journal
MICROCHIMICA ACTA
Volume 183, Issue 2, Pages 881-887Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-015-1728-9
Keywords
Atomic force microscopy; High-resolution transmission electron microcopy; Cyclic voltammetry; Nanoparticles; Cystamine; Nanofilm
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Funding
- National Natural Science Foundation of China [21375048]
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The article describes a quartz crystal microbalance (QCM) biosensor for the determination of nucleic acids via a DNA-templated assembly of silver nanoclusters (AgNCs) which represents a novel way for efficient signal amplification. A QCM was modified with probe DNA to specifically capture target DNA. Then, DNA-templated AgNCs were assembled to enhance the sensitivity of the QCM sensor via Ag(I) ions attached to the DNA skeleton, this followed by hydroquinone-induced reductive formation of the AgNCs. TEM and AFM were used to further confirm the formation of DNA-templated AgNCs. The results showed that frequency response of QCM sensor is up to 87 times larger when using this mode of amplification. A linear relationship was obtained between the frequency response and DNA concentration over the 0.6 to 130 nM range, with a 0.1 nM detection limit. In our perception, this scheme for improved sensitivity provides a straightforward and widely applicable tool for sensing DNA.
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