Journal
INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER
Volume 24, Issue 4, Pages 629-634Publisher
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/IGC.0000000000000105
Keywords
ARHI; STAT-signal transduction and activators of transcription; Autophagy; P-STAT3-phosphorylated STAT3; GFP-green fluorescence protein; IR-inhibitory rate; ERK-extracellular signal regulated protein kinase; SKOV3; GAPDH-glyceraldehyde phosphate dehydrogenase; OD-optical density; Ovarian neoplasms; STAT3; ARHI-aplasia Ras homolog member I
Categories
Funding
- Medical Science and Technology Development Foundation, Nanjing Department of Health of China [YKK10037]
Ask authors/readers for more resources
Objective Aplasia Ras homolog member I (ARHI) is associated with human ovarian cancer (HOC) growth and proliferation; however, the mechanisms are unclear. The purpose of this study was to investigate ARHI effects in HOC SKOV3 cells. Methods We transfected SKOV3 cells with PIRES2-EGFP-ARHI and measured growth inhibition rates, cell cycle distribution, apoptosis rates, and expression of P-STAT3 (phosphorylated signal transduction and activators of transcription 3) and P-ERK (phosphorylated extracellular signal regulated protein kinase). Results Our data showed significant inhibition of growth, significantly increased S-phase arrest and apoptosis rates, and reduction of P-STAT3 and P-ERK1/2 expression levels. Conclusions We propose the mechanism may involve ARHI-induced phosphorylation of ERK1/2 and STAT3 protein kinases, thereby blocking proliferation signaling pathways, to induce HOC SKOV3 apoptosis.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available