Article
Multidisciplinary Sciences
Michelle Su, Michelle H. Davis, Jessica Peterson, Claudia Solis-Lemus, Sarah W. Satola, Timothy D. Read
Summary: VISA resistance is typically caused by accumulation of chromosomal mutations in six common genes, but the effect of these mutations on fitness and resistance contribution varies among different strain backgrounds.
Article
Biochemical Research Methods
Yu-Ching Liu, Jang-Jih Lu, Lee-Chung Lin, Hsiao-Chuan Lin, Chao-Jung Chen
Summary: The rapid identification of methicillin-sensitive Staphylococcus aureus (MSSA), heterogeneous vancomycin-intermediate S. aureus (hVISA), and vancomycin-intermediate S. aureus (VISA) is crucial for accurate treatment, timely intervention, and outbreak prevention. Proteomics analysis of 90 S. aureus isolates led to the discovery of protein biomarkers that can discriminate among MSSA, hVISA, and VISA strains, with two VISA, two hVISA, and one MSSA protein biomarkers identified and verified for high sensitivities and specificities. Data is available via MassIVE with identifier MSV000085776.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Infectious Diseases
Reena Lamichhane-Khadka, Santosh Dulal, Jesus A. Cuaron, Richard Pfeltz, Sushim Kumar Gupta, Brian J. Wilkinson, John E. Gustafson
Summary: Comparative genomic sequencing of two laboratory-derived vancomycin-intermediate Staphylococcus aureus (VISA) strains (MM66-3 and MM66-4) revealed unique mutations compared to the parent strain MM66. Transcriptional profiling showed similar changes in both VISA strains, including reduced autolysis, growth in the presence of salt, and virulence factor activity. Mutations in the key gene apt may play a role in altered adenine metabolism in VISA strains.
Article
Multidisciplinary Sciences
Yifan Rao, Huagang Peng, Weilong Shang, Zhen Hu, Yi Yang, Li Tan, Ming Li, Renjie Zhou, Xiancai Rao
Summary: The WalK(S221P) mutation enhances vancomycin resistance and decreases virulence in Staphylococcus aureus.
JOURNAL OF ADVANCED RESEARCH
(2022)
Review
Immunology
Daniel J. Minter, Ayesha Appa, Henry F. Chambers, Sarah B. Doernberg
Summary: This review aims to provide a comprehensive overview of the evaluation and management of Staphylococcus aureus bacteremia, with a special focus on areas where the highest level of evidence is lacking to inform best practices.
CLINICAL INFECTIOUS DISEASES
(2023)
Article
Microbiology
Alina Dietrich, Ursula Steffens, Mike Gajdiss, Anna-Lena Boschert, Jana Katharina Droege, Christiane Szekat, Peter Sass, Imran T. Malik, Jan Bornikoel, Laura Reinke, Boris Macek, Mirita Franz-Wachtel, Kay Nieselt, Theresa Harbig, Kirstin Scherlach, Heike Broetz-Oesterhelt, Christian Hertweck, Hans-Georg Sahl, Gabriele Bierbaum
Summary: Resistance to antibiotics is a growing problem and alternative antimicrobial therapies are needed. Cervimycin, a polyketide antibiotic, shows promising activity against multidrug-resistant staphylococci and vancomycin-resistant enterococci. This study identified the Clp system and the essential kinase WalK as mutational hotspots for cervimycin resistance in Staphylococcus aureus. Furthermore, cervimycin-resistant strains exhibited vancomycin-intermediate resistance phenotype, suggesting a role of cervimycin in perturbing the bacterial cell envelope.
MICROBIOLOGY SPECTRUM
(2022)
Review
Infectious Diseases
Chienhsiu Huang, Ihung Chen, Lichen Lin
Summary: Combination therapy with daptomycin plus ceftaroline may reduce mortality in patients with methicillin-resistant Staphylococcus aureus bacteremia. The combination therapy group had similar in-hospital mortality, duration of bacteremia, and adverse event rate compared to the monotherapy group, but had lower bacteremia recurrence.
Article
Infectious Diseases
Jin Liang, Yuanfang Hu, Mingxia Fu, Na Li, Fengxia Wang, Xiaojun Yu, Bing Ji
Summary: The study investigated the molecular epidemiology of Staphylococcus aureus (S. aureus) in patients from the Yellow River Delta region and examined antibiotic resistance profiles in different genetic backgrounds. Antibiotic susceptibility testing, spa typing, and agr typing were conducted on all 204 strains. The results identified 39 MRSA strains and 22 hVISA isolates. The majority of MRSA isolates belonged to agrI and were associated with type IV SCCmec. The most common genotypes among MRSA population were ST59-t437-agrI-IVa and ST72-t2431-agrI-IVF. The study highlights the genetic diversity and drug resistance patterns among MSSA strains exhibiting phenotypic hVISA.
INFECTION AND DRUG RESISTANCE
(2023)
Article
Infectious Diseases
M. Sreejisha, Shalini Shenoy Mulki, Suchitra Shenoy, Biranthabail Dhanashree, M. Chakrapani, Gopalakrishna Bhat
Summary: This study investigated infections caused by methicillin resistant Staphylococcus aureus (MRSA) in diabetic and non-diabetic patients in four tertiary care hospitals in Coastal Karnataka, South India, with a focus on heterogeneous vancomycin intermediate S. aureus (hVISA). The results showed that the rate of hVISA among all MRSA isolates was 6.4%, and the risk of hVISA infection was three times higher in diabetic patients.
INFECTION AND DRUG RESISTANCE
(2023)
Article
Microbiology
Shawna McCallin, Carmen Menzi, Swenja Lassen, Jean Daraspe, Frank Oechslin, Philippe Moreillon
Summary: In Vancomycin-intermediate Staphylococcus aureus (VISA), exposure to vancomycin may lead to cross-resistance to phage. VISA strains with intermediate levels of vancomycin resistance showed decreased sensitivity to phage. Serial passaging with vancomycin induced reduced vancomycin susceptibility and phage sensitivity. Understanding the interference between antibiotic and phage resistance mechanisms is important for designing effective therapeutic regimens.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Multidisciplinary Sciences
Yong Chan Kim, Heun Choi, Young Ah Kim, Yoon Soo Park, Young Hee Seo, Hyukmin Lee, Kyungwon Lee
Summary: Understanding the risk factors and microbiological features in recurrent E. coli BSI can aid clinicians in their management planning. Data from 2017 to 2018 were collected to assess antimicrobial resistance rates and molecular epidemiology. Risk factors for recurrence included vascular catheterization, ESBL phenotype, and high SOFA score. The highest proportion of ST131 and ESBL genotype was observed in early onset recurrent BSI. Whole genome sequencing revealed the presence of various virulence factors, resistant genes, and plasmid types in all types of BSI. This knowledge is important for clinicians in managing recurrent E. coli BSI.
Article
Microbiology
Ahmad Mourad, Thomas L. Holland, Nicholas A. Turner
Summary: This commentary reflects on the impact of clopidogrel use on clinical outcomes in patients with Staphylococcus aureus bacteremia, as analyzed in a recent study.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Review
Biochemistry & Molecular Biology
Isabella Hernandez-Aristizabal, Ivan Dario Ocampo-Ibanez
Summary: The emergence of bacteria resistant to conventional antibiotics, especially VRSA and VISA strains, poses a serious threat to global health. Developing new antimicrobial alternatives, such as antimicrobial peptides (AMPs), is urgently needed as a promising therapeutic strategy to combat resistant strains. Evaluating natural, artificial, and synthetic AMPs against VRSA and VISA strains shows great potential for clinical application.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
Abraham G. Moller, Robert A. Petit III, Michelle H. Davis, Timothy D. Read
Summary: This study developed a method for rapid detection of vancomycin-intermediate resistance in Staphylococcus aureus through long-read genomic sequencing. The method can identify VISA genotypes and incorporate a comprehensive database of VISA genetic determinants.
MICROBIOLOGY SPECTRUM
(2023)
Article
Infectious Diseases
Kawisara Krasaewes, Saowaluck Yasri, Phadungkiat Khamnoi, Romanee Chaiwarith
Summary: The study shows that implementing a management protocol for patients with Staphylococcus aureus bloodstream infection can improve quality of care, but the mortality rate did not decrease.
Article
Infectious Diseases
Sabrina Di Gregorio, Silvina Fernandez, Beatriz Perazzi, Natalia Bello, Angela Famiglietti, Marta Mollerach
INFECTION GENETICS AND EVOLUTION
(2016)
Article
Microbiology
Melina Herrera, Sabrina Di Gregorio, Silvina Fernandez, Graciela Posse, Marta Mollerach, Jose Di Conza
ANNALS OF CLINICAL MICROBIOLOGY AND ANTIMICROBIALS
(2016)
Article
Microbiology
Silvina Fernandez, Camila Ledo, Santiago Lattar, Mariangeles Noto Llana, Andrea Mendoza Bertelli, Sabrina Di Gregorio, Daniel O. Sordelli, Marisa I. Gomez, Marta E. Mollerach
INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY
(2017)
Article
Biochemistry & Molecular Biology
Agustin Zavala, Veronica Kovacec, Gustavo Levin, Albertina Moglioni, Maria Victoria Miranda, Ernesto Garcia, Laura Bonofiglio, Marta Mollerach
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
(2017)
Article
Infectious Diseases
Sabrina Di Gregorio, Silvina Fernandez, Arabela Cuirolo, Olivier Verlaine, Ana Amoroso, Dominique Mengin-Lecreulx, Angela Famiglietti, Bernard Joris, Marta Mollerach
MICROBIAL DRUG RESISTANCE
(2017)
Article
Microbiology
Monica Elisabeth Martinez, Sandra Liliana Grenon, Oscar Herminio Lopez, Lorena Beatriz Leguizamon, Marta Eugenia Mollerach, Martha Helena von Specht
REVISTA ARGENTINA DE MICROBIOLOGIA
(2017)
Article
Microbiology
Andrea Pacha, Ramiro Luna Cian, Laura Bonofiglio, Melisa Solari, Virginia Strada, Mariana Suarez, Laura Vigliarolo, Carina Tersigni, Marta Mollerach, Horacio Lopardo
REVISTA ARGENTINA DE MICROBIOLOGIA
(2017)
Article
Infectious Diseases
Claudia Barberis, Mabel Budia, Susana Palombarani, Carlos Hernan Rodriguez, Maria Soledad Ramirez, Barbara Arias, Laura Bonofiglio, Angela Famiglietti, Marta Mollerach, Marisa Almuzara, Carlos Vay
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2017)
Letter
Microbiology
A. Montilla, A. Zavala, R. Caceres Caceres, R. Cittadini, C. Vay, G. Gutkind, A. Famiglietti, L. Bonofiglio, M. Mollerach
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2014)
Article
Infectious Diseases
S. Fernandez, L. de Vedia, M. J. Lopez Furst, N. Gardella, S. Di Gregorio, M. C. Ganaha, S. Prieto, E. Carbone, N. Lista, F. Rotrying, M. E. Stryjewski, M. Mollerach
INFECTION GENETICS AND EVOLUTION
(2013)
Article
Microbiology
M. Almuzara, L. Bonofiglio, R. Cittadini, C. Vera Ocampo, A. Montilla, M. del Castillo, M. S. Ramirez, M. Mollerach, C. Vay
JOURNAL OF CLINICAL MICROBIOLOGY
(2013)
Article
Multidisciplinary Sciences
Maria Jose Lopez Furst, Lautaro de Vedia, Silvina Fernandez, Noella Gardella, Maria Cristina Ganaha, Sergio Prieto, Edith Carbone, Nicolas Lista, Flavio Rotryng, Graciana I. Morera, Marta Mollerach, Martin E. Stryjewski
Article
Infectious Diseases
Martha Helena von Specht, Noella Gardella, Clotilde Ubeda, Sandra Grenon, Gabriel Gutkind, Marta Mollerach
JOURNAL OF INFECTION IN DEVELOPING COUNTRIES
(2014)
Article
Microbiology
Maria Sol Haim, Marta Mollerach, Gary Van Domselaar, Sergio A. Teves, Jose Degrossi, Silvia T. Cardona
GENOME ANNOUNCEMENTS
(2016)