Journal
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
Volume 46, Issue 12, Pages 2460-2466Publisher
WILEY
DOI: 10.1111/j.1365-2621.2011.02768.x
Keywords
Free radical scavenging activity; hydrolysate; lipid peroxidation; molecular weight distribution; tuna head
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Funding
- Guangdong Natural Science Foundation of China [10152408801000031]
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The antioxidant activities of tuna head protein hydrolysate (THPH) prepared with Alcalase were evaluated. THPH showed evident radical scavenging activity in a dose-dependent manner with the IC50 values for 1,1-diphenyl-2-pycrylhydrazyl (DPPH), superoxide and hydroxyl radicals being 1.34, 1.20 and 2.84 mg mL-1, respectively, and its reducing power was 0.948 at 12.5 mg mL-1. THPH showed good inhibitory activity in soybean oil peroxidation after accelerated oxidation at 60 degrees C, and the oils with 0.01%, 0.05% and 0.1% THPH had significantly (P < 0.05) lower peroxide values than the control, after storage at 60 degrees C. Moreover, the inhibited oxidation effect of 0.1% THPH was similar to that of 0.01% butylated hydroxytoluene (BHT). The molecular weight distribution of THPH revealed that 70.5% of the total amount was peptides with molecular weight lower than 5000 Da, composing mostly of low molecular weight peptides located at 10202585 Da (30.78%) and 2411020 Da (37.15%).
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