Review
Biochemistry & Molecular Biology
Luke F. Vistain, Savas Tay
Summary: The inability to make broad, minimally biased measurements of a cell's proteome is a major bottleneck in understanding gene expression and cellular phenotype. Current methods for single-cell proteomic measurements typically focus on either absolute quantification of a small number of proteins or highly multiplexed protein measurements, with advancements in microfluidics and output encoding enabling major improvements. Emerging technologies like single-cell mass spectrometry may provide unbiased measurement of cellular proteomes.
TRENDS IN BIOCHEMICAL SCIENCES
(2021)
Article
Chemistry, Analytical
Li Wu, Shihan Xu, Jingang Wang, Amy L. Paguirigan, Jerald P. Radich, Yuling Qin, Daniel T. Chiu
Summary: Researchers have developed a single cell dispenser inspired by electrohydrodynamic jet printing, which can be used for single-cell multiomics studies, achieving accurate droplet generation and single-cell dispensing through systematic parameter optimization.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Chen Dong, Ya-ru Miao, Rui Zhao, Mei Yang, An-yuan Guo, Zhong-hui Xue, Teng Li, Qiong Zhang, Yanfeng Bao, Chen Shen, Chi Sun, Ying Yang, Xi-xi Gu, Yi Jin, Rong Li, Min Xu, Jia-xin Guo, Zhi-ying Zong, Wei Zhou, Mei He, Dan-ni Wang, Jian-you Su, Xiao-ming Zhang, Xu-hui Zeng, Jian-lin Gao, Zhi-feng Gu
Summary: This study reveals the immune remodeling pattern in healthy aging, characterized by the quantitative increase and functional reinforcement of cytotoxic T cells.
Article
Cell Biology
Conner Kummerlowe, Simutanyi Mwakamui, Travis K. Hughes, Nolawit Mulugeta, Victor Mudenda, Ellen Besa, Kanekwa Zyambo, Jessica E. S. Shay, Ira Fleming, Marko Vukovic, Ben A. Doran, Toby P. Aicher, Marc H. Wadsworth, Juliet Tongue Bramante, Amiko M. Uchida, Rabiah Fardoos, Osaretin E. Asowata, Nicholas Herbert, Omer H. Yilmaz, Henrik N. Kloverpris, John J. Garber, Jose Ordovas-Montanes, Zev J. Gartner, Thomas Wallach, Alex K. Shalek, Paul Kelly
Summary: Environmental enteropathy (EE) is a subclinical condition of the small intestine that is highly prevalent in low- and middle-income countries. This study used single-cell RNA sequencing to analyze the cellular and molecular correlates of EE. The results showed that the transcriptional signatures of EE may be driven by an increased abundance of surface mucosal cells and identified dysregulated signaling pathways and increased proinflammatory cytokine gene expression in EE epithelium.
SCIENCE TRANSLATIONAL MEDICINE
(2022)
Article
Chemistry, Analytical
Chengxin Wu, Xue Men, Meijun Liu, Yujia Wei, Xing Wei, Yong-Liang Yu, Zhang-Run Xu, Ming-Li Chen, Jian-Hua Wang
Summary: A 2D flow cytometry platform called CytoLM Plus was developed for multi-parameter single-cell analysis. This platform combines fluorescence and side scattering optical detection with ICP-MS detection for a comprehensive analysis of single cells. The performance of CytoLM Plus was evaluated using fluorescent microspheres and cells, and it showed comparable results to a commercial flow cytometer.
ANALYTICAL CHEMISTRY
(2023)
Article
Optics
Gwinky G. K. Yip, Michelle C. K. Lo, Wenwei Yan, Kelvin C. M. Lee, Queenie T. K. Lai, Kenneth K. Y. Wong, Kevin K. Tsia
Summary: The new high-speed quantitative phase imaging (QPI) technique presented in this study combines time-interleaved phase-gradient FACED image detection, enabling fast imaging and cell analysis. Integration with microfluidic technology allows for high-speed, high-resolution imaging of cell phenotypes.
Article
Chemistry, Analytical
Chengxin Wu, Xing Wei, Xue Men, Xuan Zhang, Yong-Liang Yu, Zhang-Run Xu, Ming-Li Chen, Jian-Hua Wang
Summary: CytoLM is a two-dimensional cytometry platform developed for single-particle and single-cell sampling and analysis, featuring high sensitivity and high temporal resolution. It combines VCCS-LIF and ICP-MS technologies to enable high-throughput, multi-dimensional, and multi-parameter characterization of particles and cells, showing vast potential in life science analysis.
ANALYTICAL CHEMISTRY
(2021)
Review
Chemistry, Analytical
Majood Haddad, Alex N. Frickenstein, Stefan Wilhelm
Summary: Understanding nanoparticle-cell interactions at single-nanoparticle and single-cell resolutions is crucial to improving the design of next-generation nanoparticles for safer, more effective, and more efficient applications in nanomedicine. This review focuses on recent advances in the continuous high-throughput analysis of nanoparticle-cell interactions at the single-cell level, discussing methods such as flow cytometry and inductively coupled plasma mass spectrometry, as well as their intersection in mass cytometry. The review also explores the challenges and opportunities in current single-cell analysis approaches and proposes directions for innovation in high-throughput analysis of nanoparticle-cell interactions.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Biophysics
Douwe S. de Bruijn, Paul M. ter Braak, Dedmer B. Van de Waal, Wouter Olthuis, Albert van den Berg
Summary: The study explores the use of microfluidic single-cell impedance spectroscopy in the field of calcifying algae, distinguishing between calcified and decalcified algae based on the opacity ratio of high frequency vs. low frequency impedance. Results show that calcified and decalcified coccolithophores can be discriminated with an accuracy of 94.1% using opacity. The correlation between opacity and cell height, supported by FEM simulations, indicates that differences in cell density can explain the distinctions in cell height and opacity measurement.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Multidisciplinary Sciences
Shobana Stassen, Gwinky G. K. Yip, Kenneth K. Y. Wong, Joshua W. K. Ho, Kevin K. Tsia
Summary: Scalable trajectory inference for multi-omic single cell datasets is challenging due to the complexity of topologies. The VIA method presented in this study overcomes these limitations by using lazy-teleporting random walks to accurately reconstruct complex cellular trajectories.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Analytical
Shu-Ting Xu, Cheng Yang, Xiu-Ping Yan
Summary: The study introduces an all-in-one system for rapid single-HeLa-cell screening and in-depth profiling of cellular metabolites and drugs. Utilizing zinc oxide nanothorn-decorated filters for efficient online lysis of cell membrane, the system showed promising results for subcellular discrimination and signal response improvements. This system has the potential for high-coverage single-cell metabolome analysis in cell biology and cancer research.
ANALYTICAL CHEMISTRY
(2021)
Article
Biophysics
A. K. M. Arifuzzman, Norh Asmare, Tevhide Ozkaya-Ahmadov, Ozgun Civelekoglu, Ningquan Wang, A. Fatih Sarioglu
Summary: Characterization and identification of cell populations using surface markers are crucial in various applications. However, conventional techniques are limited by the need for immunolabeling, expensive instruments, and skilled operators. This study introduces an autonomous microchip that can electronically quantify the immunophenotypical composition of a cell suspension. The microchip captures different cell subtypes in separate microfluidic chambers and electrically monitors all on-chip activity, allowing real-time determination of subpopulation fractions. The capability to analyze immune cells without labels has the potential to enable remote testing and quality control in cell manufacturing.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Biochemistry & Molecular Biology
Michael J. Geuenich, Jinyu Hou, Sunyun Lee, Shanza Ayub, Hartland W. Jackson, Kieran R. Campbell
Summary: Astir is a probabilistic model that assigns cells to cell types by integrating prior knowledge of marker proteins, enabling annotations at the million-cell scale with scalability and robustness.
Review
Biotechnology & Applied Microbiology
Mohammad Tajik, Mahroo Baharfar, William A. Donald
Summary: Recent advances in mass spectrometry (MS) have allowed for the measurement of multiple molecules in single cells. This is crucial for understanding the molecular heterogeneity and function of individual cells. In this review, we discuss different methods of single-cell MS, including high-throughput, targeted, mass cytometry-based approaches, and antibody-free methods, for detailed profiling of the proteome and metabolome of single cells. We also explore the advantages and disadvantages of these methods and the challenges and opportunities for further improvements in single-cell MS. These methods have found applications in biomedical research, such as revealing tumor heterogeneity and conducting high-content drug screening.
TRENDS IN BIOTECHNOLOGY
(2022)
Review
Biochemical Research Methods
Sofani Tafesse Gebreyesus, Gul Muneer, Chih-Cheng Huang, Asad Ali Siyal, Mihir Anand, Yu-Ju Chen, Hsiung-Lin Tu
Summary: Single-cell proteomics (SCP) enables a more holistic view of biological details by revealing phenotypic heterogeneity and functional outcomes upon signaling activation. Microfluidic-based strategies have become the preferred method for SCP analysis, and they improve the sensitivity, robustness, and reproducibility of SCP methods. The critical roles of microfluidics technologies are expected to expand further in advancing SCP analysis.
Article
Biochemical Research Methods
K. Ramki, G. Thiruppathi, Selva Kumar Ramasamy, P. Sundararaj, P. Sakthivel
Summary: A chromone-based ratiometric fluorescent probe L2 was developed for the selective detection of Hg(II) in a semiaqueous solution. The probe exhibited enhanced fluorescence in its aggregated state and even higher fluorescence when chelated with Hg(II). The probe demonstrated high sensitivity and specificity for Hg(II) detection and was successfully applied for imaging Hg(II) in a living model.
Article
Biochemical Research Methods
Qun Zhang, Rui Yang, Gang Liu, Shiyan Jiang, Jiarui Wang, Juqiang Lin, Tingyin Wang, Jing Wang, Zufang Huang
Summary: This research aims to develop a cost-effective and portable method for measuring creatinine levels using the enhanced Tyndall effect phenomenon. The method offers a promising solution for monitoring renal healthcare in resource-limited settings.