Journal
METHODS
Volume 88, Issue -, Pages 122-132Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymeth.2015.05.025
Keywords
Super-resolution microscopy; Localization microscopy; Imaging artefacts; Localization error
Funding
- Hungarian Brain Research Program [KTIA_13_NAP-A-I/14]
- Marie Curie Integration Grant [PCIG13-GA-2013-618273]
- EPSRC
- Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences
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Interpretation of high resolution images provided by localization-based microscopy techniques is a challenge due to imaging artefacts that can be categorized by their origin. They can be introduced by the optical system, by the studied sample or by the applied algorithms. Some artefacts can be eliminated via precise calibration procedures, others can be reduced only below a certain value. Images studied both theoretically and experimentally are qualified either by pattern specific metrics or by a more general metric based on fluorescence correlation spectroscopy. (C) 2015 Elsevier Inc. All rights reserved.
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