Journal
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
Volume 173, Issue -, Pages 81-88Publisher
ELSEVIER
DOI: 10.1016/j.ijfoodmicro.2013.12.019
Keywords
Bacteriocins; Carnobacterium; Carnobacteriocins; Purification; Listeria spp
Categories
Funding
- Sao Paulo Research Foundation (FAPESP) [2009/11134-2, 2009/10530-1]
- Natural Sciences and Engineering Research Council of Canada (NSERC)
- Griffith Laboratories Canada
- Canada Research Chair in Bioorganic and Medicinal Chemistry
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Carnobacterium maltaromaticum C2, isolated from Brazilian smoked fish (Surubim, Pseudoplatystoma sp.), was found to exert antimicrobial activity against Listeria monocytogenes, an important foodborne pathogen. In this study, the bacteriocins produced by C. maltaromaticum 0 were purified via an extraction with XAD-16 resin, a C18 solid phase extraction, followed by reversed-phase fast protein liquid chromatography. The purified active fractions were characterized using tandem mass spectrometry, permitting the identification of multiple bacteriocins. Carnobacteriocins BM1, B1, and a variant of carnobacteriocin B2 were all found, providing much of the antilisterial activity. Additionally, we herein report the first isolation of the previously predicted antimicrobial peptide carnobacteriocin X. Moreover, C. maltaromaticum C2 produces a novel two-component lantibiotic, termed carnolysin, homologous to enterococcal cytolysin. This lantibiotic is antimicrobially inactive when tested against the non-bacteriocinogenic strain C. maltaromaticum A9b -, likely requiring an additional proteolytic cleavage to reach maturity. (C) 2013 Elsevier B.V. All rights reserved.
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