Journal
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
Volume 166, Issue 1, Pages 28-33Publisher
ELSEVIER
DOI: 10.1016/j.ijfoodmicro.2013.06.015
Keywords
Alicyclobacillus spp.; Immunomagnetic separation (IMS); Enzyme-linked immunosorbent assay (ELISA); Detection; Apple juice
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Funding
- National Science & Technology Support Program [2012BAK17B06, 2012BAD31B01]
- National Natural Science Foundation of China [31071550, 31171721]
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The immunomagnetic separation (IMS) technique was used in combination with an enzyme-linked immunosorbent assay (ELISA) procedure to shorten the total analysis time and improve the sensitivity for the detection of Alicyclobacillus spp. in apple juice samples. The specificity of IMS-ELISA for twenty strains of Alicyclobacillus spp. and eighteen strains of non-Alicyclobacillus spp. was determined and there was little cross-reaction with non-Alicyclobacillus strains. Artificially contaminated apple juice with different concentrations of Alicyclobacillus acidoterrestris was detected by IMS-ELISA, and the detection limit of the assay in apple juice was 10(3) CFU/mL. Furthermore, the sample inoculated with 1 CFU/mL of A. acidoterrestris could be detected as positive after incubation for 24 h. The IMS-ELISA described, allows for the identification of suspect positive samples within 3 h of testing versus 3-5 days required by standard culture methods while significantly reducing the materials and labor required for the detection of Alicyclobacillus spp. in apple juice samples. As compared with the standard culture method performed concurrently on the same set of samples, the sensitivity, specificity and accuracy of IMS-ELISA for 102 naturally contaminated apple juice samples were 91.3%, 96.02% and 95.09%, respectively. These results demonstrated that the newly proposed IMS-ELISA procedure can be a potentially useful analytical method for the detection of Alicyclobacillus spp. in apple juice. Crown Copyright (C) 2013 Published by Elsevier B.V. All rights reserved.
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