Journal
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
Volume 129, Issue 2, Pages 150-156Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijfoodmicro.2008.11.015
Keywords
Yersinia pseudotuberculosis; Food; Environmental isolates; PFGE; Ribotyping; LPS; O-antigen
Categories
Funding
- Academy of Finland ELVIRA programme [117921]
- Finnish Centre of Excellence, Microbial Food Safety Research
- Academy of Finland (AKA) [117921, 117921] Funding Source: Academy of Finland (AKA)
Ask authors/readers for more resources
Non-pathogenic Yersinia pseudotuberculosis-like strains were recovered from Finnish food and environmental samples. These strains could not be differentiated from Y. pseudotuberculosis strains using API 20E or other phenotypical tests. However, all of the strains were inv-, and virF-negative with polymerase chain reaction (PCR), while all Y. pseudotuberculosis strains used as controls were inv-positive and fresh Y. pseudotuberculosis strains were also virF-positive, indicating that the Y. pseudotuberculosis-like strains were non-pathogenic. Using pulsed-field gel electrophoresis (PFGE) with Notl enzyme and ribotyping with EcoRI and HindIII enzymes, the Y. pseudotuberculosis-like strains, which grouped genetically together, could be differentiated from true Y. pseudotuberculosis strains and from strains belonging to other sucrose-negative Yersinia species. In addition, the O-antigen gene cluster of one Y. pseudotuberculosis-like strain was characterized, and it differed from those of known Y. pseudotuberculosis serotypes. This study demonstrates that identification of Y. pseudotuberculosis from food and environmental sources using solely biochemical reactions can be incorrect, and when a strain cannot be serotyped to known Y. pseudotuberculosis serotypes, the pathogenic potential of isolates should be determined. (C) 2008 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available