Prebiotic-Like Effects of Water Soluble Chitosan on the Intestinal Microflora in Mice

In the present work, water soluble chitosan (WSC) was prepared and growth curves of Lactobacillus delbrueckii and Escherichia coli were examined in vitro. In vivo, mice were randomly divided into three groups (10 mice in each group), treated with water, 1 g/kg WSC and 0.25 g/kg of WSC respectively for 24 days. Subsequently, levofloxacin (65 mg/kg) was given for 6 days. The dynamic changes of bacterial communities were evaluated by denaturing gradient gel electrophoresis periodically. L. delbrueckii and E. coli have been inhibited by WSC in vitro. The bacteria from the genera of Bacteroides, Lactobacillus, Enterococcus and Devosia were dominant gut flora in the intestinal tract of mice. The growth of Lactobacillus has been dramatically stimulated, whereas Enterococcus faecium and Parabacteroides distasonis which were opportunistic bacterial pathogens have been inhibited. So WSC can be considered as a new food supplement to protect the intestinal microflora and regulate imbalance.