Article
Biochemistry & Molecular Biology
Zeen Aref, Paul H. A. Quax
Summary: Neovascularization plays a crucial role in restoring blood flow after ischemia in peripheral arterial disease, with both angiogenesis and arteriogenesis contributing to functional improvements. However, distinguishing the specific contributions of each process can be challenging. Through the in vivo Matrigel plug assay in genetic deficient mice, we were able to evaluate the effects of various gene deficiencies on angiogenesis, providing insights into the role of different genes in this process. Our study suggests that the in vivo Matrigel plug assay may be superior to the murine hind limb ischemia model in evaluating the angiogenic response post ischemia.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Medicine, Research & Experimental
Aurora Laborda-Illanes, Lidia Sanchez-Alcoholado, Daniel Castellano-Castillo, Soukaina Boutriq, Isaac Plaza-Andrades, Lucia Aranega-Martin, Jesus Peralta-Linero, Emilio Alba, Alicia Gonzalez-Gonzalez, Maria Isabel Queipo-Ortuno
Summary: This study aimed to develop rapid, economic, high capacity, and easy handling angiogenesis assays to test the antiangiogenic effects of melatonin and determine its most effective dose to inhibit angiogenic sprouting induced by VEGF and MCF-7. The results demonstrated that melatonin had an inhibitory effect on angiogenesis, and this inhibition was dose-dependent.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Biology
Stephen B. Waters, Joseph R. Dominguez, Hyun-Dong Cho, Nicolene A. Sarich, Asrar B. Malik, Kaori H. Yamada
Summary: Research demonstrates that KAI has protective effects in VEGF-A-induced vascular leakage and cancer metastasis, while an EC-specific KIF13B knockout mouse model reveals the role of KIF13B in regulating pathological angiogenesis, vascular leakage, tumor growth, and cancer metastasis induced by VEGF-A.
LIFE SCIENCE ALLIANCE
(2021)
Article
Cell Biology
Xuechun Li, Yang Yu, Renyue Wei, Yimei Li, Jiawei Lv, Zhonghua Liu, Yu Zhang
Summary: PSCs are a promising source of ECs for cardiovascular disease treatment due to ethical concerns and immune rejection risks associated with ESCs. iPSCs offer a solution for these issues. Swine serve as a suitable model for human, but there is limited research on EC differentiation of piPSCs. This study successfully differentiated piPSCs into ECs and demonstrated their functional properties, showing potential for cell transplantation and mechanistic studies in endothelial dysfunction.
Review
Biochemistry & Molecular Biology
Wa Du, Lu Ren, Milton H. Hamblin, Yanbo Fan
Summary: The process of angiogenesis, involving the formation of new blood vessels from existing ones, is critical in various physiological and pathological conditions. Endothelial cell metabolism, particularly glycolysis, has been shown to play a significant role in angiogenesis, with various regulators identified to modulate this process. Understanding the metabolic regulation in endothelial cells could lead to potential therapeutic strategies for angiogenesis-related diseases.
Article
Peripheral Vascular Disease
Laura M. Pillay, Joseph J. Yano, Andrew E. Davis, Matthew G. Butler, Keith A. Barnes, Vanessa L. Reyes, Daniel Castranova, Aniket Gore, Matthew R. Swift, James R. Iben, Amber N. Stratman, Brant M. Weinstein, Madeleine Kenton, Megan O. Ezeude, Jong S. Park
Summary: This study investigates the role of RHOA in vascular development and integrity using zebrafish as a model. The findings suggest that both excessive and insufficient RHOA activity can lead to vascular dysfunction.
Article
Multidisciplinary Sciences
Ana Martins Figueiredo, Pedro Barbacena, Ana Russo, Silvia Vaccaro, Daniela Ramalho, Andreia Pena, Aida Pires Lima, Rita Rua Ferreira, Marta Alves Fidalgo, Fatima El-Marjou, Yulia Carvalho, Francisca Ferreira Vasconcelos, Ana-Maria Lennon-Dumenil, Danijela Matic Vignjevic, Claudio Areias Franco
Summary: The study shows that endothelial tip cells use dactylopodia as the main cellular protrusion for invasion, and this process is regulated by the balance of myosin IIA and actin-related protein activity. Autonomous ablation of these proteins in endothelial cells may lead to abnormal protrusion formation, affecting angiogenesis.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Immunology
Jiajie Kuai, Chenchen Han, Wei Wei
Summary: GRK2 is a key player in various signaling networks, interacting with both GPCRs and non-GPCR substrates to regulate angiogenesis. Changes in GRK2 expression and localization have been linked to the angiogenesis process in different diseases, suggesting its potential as a target for anti-angiogenesis therapy.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Aleen Al Halawani, Lea Abdulkhalek, Suzanne M. Mithieux, Anthony S. Weiss
Summary: The study showed that the pro-angiogenic effects of tropoelastin are mediated indirectly through its action on co-cultured cells, reversing its inhibitory effect on endothelial tube formation. In vitro experiments demonstrated that tropoelastin can mimic its known in vivo pro-angiogenic effects, highlighting its value as an indirect mediator of angiogenesis.
Article
Chemistry, Analytical
Yue Wu, Yuyuan Zhou, Ratul Paul, Xiaochen Qin, Khayrul Islam, Yaling Liu
Summary: This study reports a microfluidic vessel-on-a-chip platform for recapitulating key metastatic steps of tumor cells in blood vessels and investigating the interactions between cells and vessels. Biomechanical factors like vascular surface properties and hemodynamics were found to have significant impacts on cell adhesion. Computational models based on confocal scanned 3D images were established to provide insights into tumor metastasis and inspire new ideas for anticancer therapy development.
ANALYTICAL CHEMISTRY
(2022)
Review
Biochemistry & Molecular Biology
Lucio Diaz-Flores, Ricardo Gutierrez, Maria Pino Garcia, Miriam Gonzalez-Gomez, Lucio Diaz-Flores, Jose Luis Carrasco, Juan Francisco Madrid, Aixa Rodriguez Bello
Summary: Perivascular cells, including pericytes and CD34+ stromal cells/telocytes, play an important role in angiogenesis. The behavior and changes of these cells differ depending on the type of angiogenesis, involving various cell-to-cell interactions and signaling pathways. Perivascular cells and CD34+ stromal cells/telocytes have important functions in vessel development and tissue repair, while their involvement in intussusceptive angiogenesis is limited.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Engineering, Biomedical
Ross E. B. Fitzsimmons, Ronald G. Ireland, Aileen Zhong, Agnes Soos, Craig A. Simmons
Summary: One approach to engineering viable tissues ex vivo involves using hydrogel-based microfluidics to generate microvessels, with a focus on collagen, fibrin, and collagen-fibrin co-gel formulations. Fibrin and co-gels were found to promote multicellular EC sprouting, while collagen required supplementation to elicit a migration response. Increasing collagen content in co-gel formulations led to higher compressive modulus and reliable formation of intact hydrogel-based microchannels for perfusion, making collagen-fibrin co-gels a promising option for generating vascularized tissue constructs.
BIOMEDICAL MATERIALS
(2021)
Review
Biochemistry & Molecular Biology
Ana Isabel Fraguas-Sanchez, Cristina Martin-Sabroso, Ana Isabel Torres-Suarez
Summary: The chick chorioallantoic membrane (CAM) model has become a valuable tool in pharmaceutical and biological research, allowing for the evaluation of drug toxicity, testing the efficacy of anticancer and anti-infective therapies, and conducting biodistribution and tissue engineering studies.
CURRENT MEDICINAL CHEMISTRY
(2022)
Article
Nanoscience & Nanotechnology
Dan Zhao, Mengting Liu, Jiajie Li, Dexuan Xiao, Shuanglin Peng, Qing He, Yue Sun, Qirong Li, Yunfeng Lin
Summary: The study identified that modified nanomaterials have a strong capability to promote angiogenesis, presenting new hope for addressing tissue engineering vascularization and ischemic diseases.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Oncology
Baikun Li, Ting Zhu, Xiaohong Wu, Shiyu Chen, Chen Lu, Jimin Zhu, Qinglin Li
Summary: This study aimed to investigate whether the interaction between lung cell lines and platelets (PLTs) could affect tumor angiogenesis. The results showed that co-culturing lung cell lines with PLTs enhanced the proliferation, migration, invasion, and tube formation activities of endothelial cells. Similar results were observed in a mouse model. The study also found that the VEGF/VEGFR2 signaling pathway played a key role in lung carcinoma angiogenesis.
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
(2023)
Article
Cell Biology
Gordana Smiesko, Pavle Banovic, Vera Gusman, Verica Simin, Anca Maria Cimpean, Dusan Lalosevic
JOURNAL OF MOLECULAR HISTOLOGY
(2020)
Article
Oncology
Madalin Marius Margan, Anca Maria Cimpean, Amalia Raluca Ceausu, Marius Raica
ANTICANCER RESEARCH
(2020)
Article
Oncology
Tiberiu Gabriel Adelmann, Tommaso Ciro Camerota, Amalia Raluca Ceausu, Anca Maria Cimpean, Michele Mazzanti, Marius Raica
ANTICANCER RESEARCH
(2020)
Article
Oncology
Marius Raica, Amalia Raluca Ceausu, Anca Maria Cimpean, Serban Comsa, Simona Sarb
Summary: The study investigated the expression of CLIC1, Ecad, and Pcad in breast cancer, revealing their association with molecular subtypes and the ability to define new high-risk subgroups. This stratification may explain variations in treatment response and aggressiveness observed by different authors within the same molecular subtype.
ANTICANCER RESEARCH
(2021)
Article
Oncology
Iulia Florentina Burcea, Valeria Nicoleta Nastase, Anca Maria Cimpean, Amalia Raluca Ceausu, Ionela Baciu, Cristina Capatina, Roxana Dusceac, Simona Galoiu, Dan Niculescu, Serban Radian, Raluca Trifanescu, Anda Dumitrascu, Laurentiu Catalin Cocosila, Marius Raica, Catalina Poiana
Summary: Among 32 acromegaly patients, 93.75% had macroadenomas; Ki-67 labelling index did not predict remission, while PIT-1 was positive in all specimens with weak expression in two; there was no correlation between GH serum levels and IHC expression.
ANTICANCER RESEARCH
(2021)
Article
Oncology
Serban Comsa, Amalia-Raluca Ceausu, Roxana Popescu, Anca-Maria Cimpean, Simona Sarb, Marius Raica
Summary: This study aimed to analyze the patterns of tumor vasculogenesis by generating an in vivo breast cancer environment. Results showed that tumor and embryonic vasculogenesis share a common paradigm, with CD105, AC133, and Oct3/4 playing important roles in establishing the vasculogenic and hematopoietic stage.
ANTICANCER RESEARCH
(2022)
Article
Chemistry, Multidisciplinary
Vlad Bloanca, Horia Haragus, Anca-Maria Campean, Andrei Cosma, Tiberiu Bratu, Zorin Crainiceanu
Summary: The study aimed to analyze the involvement of adipose-sourced mast cells in nerve repair. Results showed that mechanically processed adipose tissue augmented nerve repair does not seem to increase mast cell expression but may support angiogenesis in an experimental model.
APPLIED SCIENCES-BASEL
(2021)
Article
Oncology
Andrei Dan Radu-Cosnita, Alexandru Nesiu, Patricia Lorena Berzava, Simona Cerbu, Andrei Cosma, Serban Comsa, Simona Sarb, Adela Maria Ferician, Ovidiu Catalin Ferician, Anca Maria Cimpean
Summary: This study evaluated the effect of anti-CLIC1 antibodies on human cc-RCC xenografts in rabbit cornea and chick embryo CAM models. The results showed that the antibodies induced tumor necrosis and regression of tumor vasculature, suggesting a potential therapeutic strategy for RCC.
ANTICANCER RESEARCH
(2022)
Review
Pediatrics
Rodica Elena Heredea, Eugen Melnic, Laura Elena Cirligeriu, Patricia Lorena Berzava, Maria Corina Stanciulescu, Calin Marius Popoiu, Anca Maria Cimpean
Summary: This study compared the gene expression profile of involuting and recurrent infantile hemangiomas, identifying several genes with differential expression. Further research is needed to accurately map the gene expression profile and establish a gene expression-based classification for hemangiomas.
Article
Oncology
Adela Maria Ferician, Ovidiu Catalin Ferician, Andrei Dragos Cumpanas, Patricia Lorena Berzava, Alexandru Nesiu, Ariana Barmayoun, Anca Maria Cimpean
Summary: This study identified three subgroups of renal cell carcinoma (RCC) with different vascular patterns and gene expression profiles. These findings may contribute to the development of personalized targeted therapies.
CANCER GENOMICS & PROTEOMICS
(2022)
Article
Medicine, Research & Experimental
Maria-Corina Stanciulescu, Marius-Calin Popoiu, Anca Maria Cimpean, Vlad-Laurentiu David, Rodica Heredea, Simona Cerbu, Eugen-Sorin Boia
Summary: This study demonstrates for the first time the heterogeneous expression of beta 1-AR among pediatric vascular malformations, supporting the need for beta 1-AR assessment to select cases with a robust response to beta 1-selective blockers. The assessment of beta 1-AR may have a significant impact on therapeutic refinement for pediatric vascular anomalies by selecting cases with a stronger response to beta-blockers.
JOURNAL OF INTERNATIONAL MEDICAL RESEARCH
(2021)
Meeting Abstract
Cardiac & Cardiovascular Systems
A. M. Cimpean, R. Taga
Article
Medicine, Research & Experimental
Raluca Amalia Ceausu, Alexandru Ciolofan, Alexandru Blidisel, Octavian Marius Cretu, Anca Maria Cimpean, Marius Raica
Summary: This study found that metastatic cells change their phenotype during the metastatic process, which may explain why not all metastases respond similarly to therapy.
Article
Medicine, Research & Experimental
Liliana Bordean, Monica Chis, Marius Raica, Ovidiu Simion Cotoi, Amalia Raluca Ceausu, Claudiu Avram, Anca Maria Cimpean
Summary: The study found that the expression of CLIC1 in the skin of patients with rheumatoid arthritis and psoriatic arthritis is closely related to the treatment plan, with methotrexate, leflunomide, and biological therapies significantly impacting CLIC1 expression. The results support CLIC1 as a potential marker of inflammation and a tool for predicting therapy response in rheumatoid arthritis.
Article
Medicine, Research & Experimental
Andrei Radu Dan Cosnita, Marius Raica, Mihai Poenaru Sava, Anca Maria Cimpean
Summary: The study on gene expression profile of normal pig cornea revealed the lack of VEGFR2 and VEGFR3 gene expression, while MK2 and AKT1 genes were significantly overexpressed. Multiple genes in the PDGF pathway were also found to be overexpressed, indicating the avascular state of the cornea is related to the down-regulation of growth factor receptors.
