Journal
INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY
Volume 54, Issue 4, Pages 609-615Publisher
UNIV BASQUE COUNTRY UPV-EHU PRESS
DOI: 10.1387/ijdb.092929ks
Keywords
XR4SGRP2; Xenopus laevis; VEGF-A; RASGRP; vasculogenesis
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Funding
- International Cooperative Research Program (ICORP)
- Japan Science and Technology Agency
- Japan Society for the Promotion of Science (JSPS)
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Ras guanyl nucleotide-releasing protein 2 (RASGRP2), one of the Ras guanine exchange factors, is implicated as a critical regulator of inside-out integrin activation in human lymphocytes, neutrophils and platelets. However, the activities of this protein in endothelial cells remain unclear. In the current study, we identify a physiological function in blood vessel formation for XRASGRP2, which is the Xenopus ortholog of mammalian RASGRP2. XRASGRP2 overexpression induced ectopic vascular formation, and XRASGRP2-knockdown embryos showed delayed vascular development. We also investigated the upstream signaling of XRASGRP2 in endothelium formation. XRASGRP2 expression was up-regulated in the presence of VEGF-A and down-regulated following VEGF-A depletion. XRASGRP2 knockdown abolished the ectopic induction of endothelial cells by VEGF-A in the posterior ventral blood island. These results suggest that XRASGRP2 is essential for vascular formation during Xenopus development.
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