Journal
INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY
Volume 53, Issue 8-10, Pages 1569-1579Publisher
UNIV BASQUE COUNTRY UPV-EHU PRESS
DOI: 10.1387/ijdb.072351jr
Keywords
fluorescence; photobleaching; photoactivation; FRET; FLIM
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Funding
- Fundacao para a Ciencia e Tecnologia, Portugal [POCI/SAU-MMO/57700/2004]
- European Commission [LSHG-CT-2003-503259]
- Fundação para a Ciência e a Tecnologia [POCI/SAU-MMO/57700/2004] Funding Source: FCT
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How we see organisms and cells depends on the tools at our disposal. For over 150 years, biologists were forced to rely on fixed, dehydrated and stained specimens in order to guess how the living cells could function. It all changed abruptly during the last two decades when the rapid development of novel imaging techniques revolutionized the way scientists look at the structures of life alive.
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