Article
Biochemistry & Molecular Biology
Malgorzata Sobstyl, Paulina Niedzwiedzka-Rystwej, Rafal Hrynkiewicz, Dominika Bebnowska, Izabela Korona-Glowniak, Marcin Pasiarski, Barbara Sosnowska-Pasiarska, Jolanta Smok-Kalwat, Stanislaw Gozdz, Anna Sobstyl, Wojciech Polkowski, Jacek Rolinski, Ewelina Grywalska
Summary: The study demonstrated the significance of TLR2 as a biomarker in advanced stage ovarian cancer by showing significantly higher expression levels in women with more advanced OC. This research sheds light on the prognostic potential of TLR2 in developing new diagnostic approaches and increasing survival in confirmed ovarian cancer patients.
Review
Cell Biology
Elise E. Bruning, Janet K. Coller, Hannah R. Wardill, Joanne M. Bowen
Summary: TLR4 is a highly conserved protein involved in innate immunity with distinct roles in epithelial and immune cells throughout the gastrointestinal tract. While epithelial cell expression of TLR4 contributes to maintaining homeostasis, promoting immunoglobulin A production, and regulating permeability, immune cell expression is centered on maturation of dendritic cells and priming the adaptive immune system. However, further research is needed to fully understand the impact of site-specific TLR4 expression on inflammation and disease progression.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Marek Majewski, Kamil Torres, Paulina Mertowska, Sebastian Mertowski, Izabela Korona-Glowniak, Jan Korulczyk, Witold Zgodzinski, Ewelina Grywalska
Summary: Gastric cancer is a common type of cancer worldwide. Toll-like receptors (TLRs) expressed on immune cells play a role in the pathogenesis of gastric cancer. This study found that patients with gastric cancer had a higher percentage of immune cells expressing TLR2 compared to the control group. Additionally, there was a significant association between TLR2 expression and the stage of the disease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Rukmini Mukherjee, Anshu Bhattacharya, Denisa Bojkova, Ahmad Reza Mehdipour, Donghyuk Shin, Khadija Shahed Khan, Hayley Hei-Yin Cheung, Kam-Bo Wong, Wai-Lung Ng, Jindrich Cinatl, Paul P. Geurink, Gerbrand J. van der Heden van Noort, Krishnaraj Rajalingam, Sandra Ciesek, Gerhard Hummer, Ivan Dikic
Summary: Famotidine has no effect on viral replication but can modulate specific signaling pathways, reducing TLR3-dependent signaling processes and subsequent activation of IRF3 and the NF-kappa B pathway, leading to control of antiviral and inflammatory responses.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Biotechnology & Applied Microbiology
Kanako Matsunaga, Yasuhiko Komatsu
Summary: This study aimed to identify suitable lactic acid bacterial strains and media for fermentation to obtain soluble TLR2 agonists from traditional fermented foods and natural food sources. The results showed that Lactobacillus delbrueckii subsp. lactis TL24 and a combination of hot water extracts of asparagus edible stem and cow skimmed milk were the best options for this purpose. TL24 ferments containing soluble TLR2 agonists showed potential as ingredients for cosmetics.
FERMENTATION-BASEL
(2022)
Article
Immunology
Wenjun Shao, Yue Huang, Lili Wang, Penghui Li, Yan Jia, Jiewen Zhang
Summary: The study found that Fgl2 can alleviate the symptoms and damage of autoimmune myelitis by reducing TLR9 expression, indicating its potential role in the treatment of autoimmune diseases.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Nanoscience & Nanotechnology
Xiaolin Zhang, Mei Zhang, Mi Zhou, Tao Zhang, Yang Gao, Songhang Li, Yunfeng Lin, Xiaoxiao Cai
Summary: In this study, tetrahedral-framework nucleic acid (tFNA) was used as a carrier to deliver siRNA for downregulating the expression of TLR2, providing a new idea for anti-inflammatory therapy in sepsis. The results demonstrated that siRNA specifically reduced LPS-induced TLR2 elevation and reduced the release of inflammatory factors, offering potential for the prevention and treatment of sepsis.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Nanoscience & Nanotechnology
Xiaolin Zhang, Mei Zhang, Mi Zhou, Tao Zhang, Yang Gao, Songhang Li, Yunfeng Lin, Xiaoxiao Cai
Summary: Through using tFNA as carriers to deliver siRNA targeting downregulation of TLR2 expression for anti-inflammatory therapy, the study successfully reduced LPS-induced TLR2 elevation and decreased release of inflammatory factors, providing a new idea for the prevention and treatment of sepsis.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Clinical Neurology
Yun Xia, Guoxin Zhang, Liang Kou, Sijia Yin, Chao Han, Junjie Hu, Fang Wan, Yadi Sun, Jiawei Wu, Yunna Li, Jinsha Huang, Nian Xiong, Zhentao Zhang, Tao Wang
Summary: Increasing evidence suggests that microglial activation plays a crucial role in the initiation and progression of Parkinson's disease, with cell-to-cell propagation of alpha-synuclein pathology being a highlighted feature. Recent studies indicate that plasma exosomes from Parkinson's disease patients carry pathological alpha-synuclein and target microglia preferentially, suggesting their key role in investigating the transmission of alpha-synuclein by microglia.
Article
Chemistry, Applied
Burak Barut, Can Ozgur Yalcin, Yasemin Altun, Didem Akkaya, Elif Nur Barut, Hueseyin Bas, Zekeriya Biyiklioglu
Summary: In this study, compound 3a was synthesized and tested on A549 lung cancer cells. The results showed that 3a significantly reduced cell viability and induced apoptosis when irradiated with red light. The Toll-like receptor signaling pathway and related protein expression were also observed to change. This suggests that 3a can induce A549 cell death in a dose and red-light irradiation time-dependent manner and may be an effective candidate for photodynamic therapy in lung cancer treatment.
APPLIED ORGANOMETALLIC CHEMISTRY
(2023)
Article
Oncology
Antonino Di Lorenzo, Elisabetta Bolli, Roberto Ruiu, Giuseppe Ferrauto, Enza Di Gregorio, Lidia Avalle, Aurora Savino, Pietro Poggio, Irene Fiore Merighi, Federica Riccardo, Mara Brancaccio, Elena Quaglino, Federica Cavallo, Laura Conti
Summary: High expression of TLR2 is associated with poor prognosis in breast cancer patients. Using a mouse model, we demonstrate the importance of TLR2 in maintaining breast cancer stem cells and inducing regulatory T cells. TLR2 inhibitors can reduce tumor growth and enhance the efficacy of chemotherapy.
Letter
Dermatology
Takuya Takeichi, Yoshinao Muro, Masashi Akiyama
Summary: This article summarizes the study by Tagoe et al., in which they found that chronic activation of Toll-like receptor 2 leads to the development of ichthyotic skin phenotype. Their findings extend our understanding of the pathogenesis of ichthyosis caused by certain types of autoinflammation. Inspired by Tagoe et al.'s report, we suggest that their unique autosomal recessive congenital ichthyosis in vitro models share similarities with autoinflammatory keratinization diseases.
BRITISH JOURNAL OF DERMATOLOGY
(2023)
Review
Immunology
Kathryne E. Marks, Kaylin Cho, Courtney Stickling, Joseph M. Reynolds
Summary: TLR signaling is critical for immune recognition, particularly in activating and maturing cells of innate immunity. TLR2 heterodimerizes with TLR1 or TLR6 to recognize bacterial lipopeptides and danger molecules, promoting immune cell activation for infection defense. However, overactive inflammation due to inappropriate TLR2 signaling may have detrimental effects in sterile inflammation and autoimmune diseases.
Article
Biochemistry & Molecular Biology
Natsumi Ogawa, Shingo Nakajima, Kenya Tamada, Natsuki Yokoue, Haruki Tachibana, Miwa Okazawa, Takahiro Oyama, Hideaki Abe, Hiroaki Yamazaki, Atsushi Yoshimori, Akira Sato, Takanori Kamiya, Takehiko Yokomizo, Fumiaki Uchiumi, Takehiko Abe, Sei-ichi Tanuma
Summary: The spasmolytic drug trimebutine has shown potential as an anti-inflammatory agent in experiments, reducing the production of various proinflammatory factors and significantly decreasing mortality in a mouse model of sepsis. Studies have found that trimebutine exerts its anti-inflammatory effects by inhibiting TLR signaling pathways and NF-kappa B activity.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2021)
Review
Immunology
Niloofar Deravi, Mohadeseh Poudineh, Marzieh Pirzadeh, Hanie Yavarpour-Bali, Hoda Mehrabi, Gisou Erabi, Amene Saghazadeh, Nima Rezaei
Summary: Endothelial cells play a critical role in innate immune and inflammatory responses, and Toll-like receptors (TLRs) are key in activating innate mechanisms in ECs. Endothelial dysfunction may lead to tissue damage in various diseases.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Article
Obstetrics & Gynecology
Ivana Musilova, Martina Kolackova, Ctirad Andrys, Marcela Drahosova, Ivana Baranova, Marcela Chmelarova, Jaroslav Stranik, Bo Jacobsson, Marian Kacerovsky
Summary: Intra-amniotic infection and sterile IAI are associated with higher levels of NAMPT in the amniotic fluid, but not in cervical fluid or maternal serum in women with PPROM. Amniotic fluid NAMPT may serve as a marker for the invasive identification of IAI in PPROM.
JOURNAL OF MATERNAL-FETAL & NEONATAL MEDICINE
(2021)
Article
Obstetrics & Gynecology
Marian Kacerovsky, Roberto Romero, Lenka Pliskova, Radka Bolehovska, Helena Hornychova, Adela Matejkova, Hana Vosmikova, Ctirad Andrys, Martina Kolackova, Piotr Laudanski, Vera Pelantova, Bo Jacobsson, Ivana Musilova
Summary: The study found that the presence of C. trachomatis DNA in amniotic fluid in pregnancies complicated by preterm prelabor rupture of membranes (PPROM) is relatively low, and is associated with intra-amniotic infection and intra-uterine inflammatory complications. However, the presence of this DNA does not lead to intensive intra-amniotic and intra-uterine inflammatory responses or adverse short-term neonatal outcomes.
JOURNAL OF MATERNAL-FETAL & NEONATAL MEDICINE
(2021)
Article
Toxicology
Andrea Malkova, Lenka Borska, Jindra Smejkalova, Kvetoslava Hamakova, Jan Kremlacek, Tereza Svadlakova, Pavel Borsky, Drahomira Holmannova, Zdenek Fiala
Summary: The study compared four variants of Goeckerman therapy (GT) in terms of efficacy and genotoxic hazard, finding that a combination of 4% or 5% pharmaceutical crude coal tar (CCT) with UVA and UVB is an effective and acceptable treatment choice.
JOURNAL OF APPLIED TOXICOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Andrea Malkova, Tereza Svadlakova, Avni Singh, Martina Kolackova, Radka Vankova, Pavel Borsky, Drahomira Holmannova, Adam Karas, Lenka Borska, Zdenek Fiala
Summary: The study revealed a significant dose-dependent increase in DNA damage when human THP-1 cell line was exposed to two types of graphene platelets in vitro. However, there was no observed increase in cytotoxicity, immunotoxicity, or cytostasis.
Review
Chemistry, Multidisciplinary
Drahomira Holmannova, Pavel Borsky, Tereza Svadlakova, Lenka Borska, Zdenek Fiala
Summary: Carbon nanoparticles have unique properties that make them widely applicable in various fields, but they may have toxic effects on different tissue types, including reproduction and offspring development.
Article
Biochemistry & Molecular Biology
Adam Karas, Drahomira Holmannova, Pavel Borsky, Zdenek Fiala, Ctirad Andrys, Kvetoslava Hamakova, Tereza Svadlakova, Vladimir Palicka, Jan Krejsek, Vit Rehacek, Monika Esterkova, Helena Kovarikova, Lenka Borska
Summary: The study investigates potential markers of psoriasis and aging, finding increased levels of CRP, AGEs, and RAGE, and reduced levels of NAD among psoriatic patients compared to controls. There is a negative correlation between AGEs and NAD. The study suggests that psoriasis and other immune-mediated diseases contribute to accelerated aging through sustained systemic damage.
Article
Surgery
Miroslav Sirovy, Marketa Krupova, Radomir Hyspler, Alena Ticha, Martina Kolackova, Ctirad Andrys, Vera Radochova, David Astapenko, Sarka Odlozilova, Jiri Kotek, Jan Zajak, Jiri Paral
Summary: Intraperitoneally administered lipid emulsions have a protective effect on the formation of adhesions after abdominal surgery. The lubricating effect of phospholipids, the mechanical barrier of the lipid component, and the anti-inflammatory effect of fish oil contribute to this effect. The administration of lipid emulsions significantly reduces the incidence of intra-abdominal adhesions, reduces inflammation and collagen content in the adhesions, and decreases neovascularization.
ASIAN JOURNAL OF SURGERY
(2023)
Review
Chemistry, Multidisciplinary
Drahomira Holmannova, Pavel Borsky, Tereza Svadlakova, Lenka Borska, Zdenek Fiala
Summary: This review summarizes the current and future applications of carbon nanoparticles in medicine. Carbon nanoparticles, such as graphene, graphene oxide, and carbon nanotubes, have unique physicochemical properties that make them suitable for clinical use. They are mainly used in medical diagnostics, including bioimaging and chemical/metabolite detection. However, their use in therapeutics, regenerative medicine, and vaccine production is still under research due to nanoparticle toxicity concerns. Preclinical studies show great potential for diagnosis and treatment, but caution is needed before their routine clinical use.
APPLIED SCIENCES-BASEL
(2022)
Article
Biochemistry & Molecular Biology
Drahomira Holmannova, Pavel Borsky, Ctirad Andrys, Kvetoslava Hamakova, Eva Cermakova, Gabriela Poctova, Zdenek Fiala, Jindra Smejkalova, Vladimir Blaha, Lenka Borska
Summary: This study aimed to demonstrate the genotoxic potential of chronic inflammation and investigate whether the presence of both psoriasis and metabolic syndrome (MetS) increases chromosomal damage. The results showed that patients with psoriasis and MetS had a higher rate of chromosomal aberrations compared to the healthy population. This suggests an increased risk of genomic instability and carcinogenesis in these patients.
Article
Dermatology
Martin Beranek, Pavel Borsky, Zdenek Fiala, Ctirad Andrys, Kvetoslava Hamakova, Marcela Chmelarova, Helena Kovarikova, Adam Karas, Jan Kremlacek, Vladimir Palicka, Lenka Borska
Summary: This study investigated oxidation damage, telomere length, and methylation status in the DNA of peripheral blood from psoriatic patients. The results showed higher levels of oxidative damage in patients' serum DNA/RNA compared to control group. Patients' whole-cell DNA exhibited longer telomeres, especially in females, compared to controls. The difference in global DNA methylation between psoriasis cases and controls was not significant.
ANAIS BRASILEIROS DE DERMATOLOGIA
(2023)
Article
Health Care Sciences & Services
Drahomira Holmannova, Pavel Borsky, Ctirad Andrys, Jan Krejsek, Eva Cermakova, Zdenek Fiala, Kvetoslava Hamakova, Tereza Svadlakova, Helena Parova, Vit Rehacek, Gabriela Poctova, Lenka Borska
Summary: This study analyzed the inflammatory processes of psoriasis and metabolic syndrome and identified potential markers involved in their pathogenesis. The results showed that CRP and CD5L were significantly increased in patients with psoriasis and a metabolic syndrome, while levels of molecules that limit inflammation were lower, reflecting the chronic nature of the diseases and exhaustion of inhibitory mechanisms.
JOURNAL OF PERSONALIZED MEDICINE
(2022)
Article
Obstetrics & Gynecology
R. Spacek, M. Kacerovsky, C. Andrys, O. Soucek, R. Kukla, R. Bolehovska, I. Musilova
Summary: This study aimed to determine the concentration of soluble CD93 (sCD93) in amniotic fluid from pregnancies complicated by preterm prelabor rupture of membranes (PPROM) based on the presence of microbial invasion of the amniotic cavity (MIAC) and/or intra-amniotic inflammation. The results showed that intra-amniotic inflammation, regardless of the presence of MIAC, is associated with elevated levels of sCD93 in amniotic fluid.
CESKA GYNEKOLOGIE-CZECH GYNAECOLOGY
(2022)
Article
Obstetrics & Gynecology
Ondrej Soucek, Marian Kacerovsky, Ivana Kacerovska Musilova, Jaroslav Stranik, Rudolf Kukla, Radka Bolehovska, Ctirad Andrys
Summary: The study aimed to evaluate CD36 concentrations in amniotic fluid in pregnancies complicated by PTL and PPROM with respect to the presence of intra-amniotic infection. In the PPROM group, women with intra-amniotic infection had higher CD36 concentrations compared to those without infection. In PTL pregnancies, there was no significant difference in CD36 concentration with respect to the presence of intra-amniotic infection.
JOURNAL OF MATERNAL-FETAL & NEONATAL MEDICINE
(2023)
Review
Biochemistry & Molecular Biology
Drahomira Holmannova, Pavel Borsky, Helena Parova, Tereza Stverakova, Milan Vosmik, Libor Hruska, Zdenek Fiala, Lenka Borska
Summary: Aging is a natural process that involves molecular, cellular, and tissue-level changes, which can increase the risk of diseases like cancer. The hallmarks of aging, including genomic and non-genomic changes, play a significant role in aging and cancer. Understanding the non-genomic hallmarks and their relationship with aging and cancer could help develop approaches to slow down aging and prevent cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
