Article
Multidisciplinary Sciences
Stephanie L. Rellick, Gangqing Hu, Debra Piktel, Karen H. Martin, Werner J. Geldenhuys, Rajesh R. Nair, Laura F. Gibson
Summary: In this study, an in vitro cell model was developed to investigate a population of treatment-refractory cells in B-cell acute lymphoblastic leukemia (ALL) characterized by dormant, chemotherapy-resistant tumor cells. Through RNA-Seq analysis, the transcriptional signature of these cells was characterized and found to be similar to expression patterns in MRD cells from patients. Genes and signaling pathways common between these cells were identified as potential therapeutic targets for future studies.
SCIENTIFIC REPORTS
(2021)
Article
Hematology
Hannah Kinoshita, Mamatha Mandava, Mariah Jensen-Wachspress, Haili Lang, Elisabeth Joy, Jay Tanna, Chase D. McCann, Samuel O'Brien, Sianna Burnett, Abeer Shibli, Fahmida Hoq, Monica Bhatia, Patrick J. Hanley, Blachy Davila Saldana, Kris M. Mahadeo, Catherine M. Bollard, Michael D. Keller, Allistair Abraham
Summary: Hematopoietic stem cell transplantation (HSCT) is increasingly used to treat sickle cell disease (SCD). However, this treatment increases the risk of viral infection. This study explores the use of virus-specific T-cell (VST) therapies after HSCT to prevent or treat viral infections in SCD patients.
Article
Hematology
Chen Wang, Ravi Nistala, Min Cao, De-Pei Li, Yi Pan, Mojgan Golzy, Yuqi Cui, Zhenguo Liu, XunLei Kang
Summary: This study found that SHP-1 responds to ischemic stress in hematopoietic stem cells (HSC) and progenitor cells (HSPC) by regulating TGF-beta 1 expression. SHP-1 deficiency leads to irreversible activation of HSPCs in the bone marrow and increased circulating HSPCs in peripheral blood after limb ischemia. Restoration of normal TGF-beta 1 expression is important for limb repair and function recovery.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2023)
Article
Oncology
Bianca Nowlan, Elizabeth D. Williams, Michael Robert Doran
Summary: The study evaluated the impact of partially humanizing mouse bone marrow with human bone marrow-derived stromal cells on human prostate cancer cell behavior. Results showed that PC-3-DsRed cells preferentially populated bones in mice that had received human BMSC, and there were more bone lesions in these mice.
Article
Hematology
Gustavo Falero-Diaz, Catarina de A. Barboza, Felipe Pires, Maeva Fanchin, Jingjing Ling, Zachary M. Zigmond, Anthony J. Griswold, Laisel Martinez, Roberto Vazquez-Padron, Omaida C. Velazquez, Roberta M. Lassance-Soares
Summary: This study found that functional reprogramming of monocytes after ischemia training improves blood flow and arteriogenesis after hindlimb ischemia. The changes in metabolism and gene expression may contribute to this beneficial effect.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Sara El-Gindy, Maram Farouk Obeid, Kareim Mostafa Elbatouty, Elham Elshaboury, Ehab Hassanien
Summary: The study investigated the use of autologous BMMNCs for cell therapy in improving bone cavity healing, with the MNC group showing the best healing potential. This method is believed to surpass conventional therapies in enhancing the healing of bone cavities in vivo.
Article
Cell & Tissue Engineering
Femke Christina Ching-Chuan van Rhijn-Brouwer, Hendrik Gremmels, Krista Den Ouden, Martin Teraa, Joost Ougust Fledderus, Marianne Christina Verhaar
Summary: Effective treatments for chronic limb-threatening ischemia are lacking. This study compared the therapeutic effects of human bone marrow mononuclear cells (MNCs) and bone marrow-derived mesenchymal stromal cells (MSCs). The results showed that intramuscular injection of human BM MSCs significantly improved limb perfusion in the murine hindlimb ischemia model, while injection of BM MNCs did not improve perfusion.
STEM CELLS AND DEVELOPMENT
(2022)
Article
Hematology
Swetha Kambhampati, Ying Sheng, Chiung-Yu Huang, Sophia Bylsma, Mimi Lo, Vanessa Kennedy, Kelsey Natsuhara, Thomas Martin, Jeffrey Wolf, Nina Shah, Sandy W. Wong
Summary: B-cell maturation antigen-targeted chimeric antigen receptor T-cell therapy is an effective treatment for relapsed refractory multiple myeloma. This study analyzed the infection outcomes of 55 MM patients treated with BCMA CAR-T and found that despite multiple risk factors, severe infections were relatively rare.
Article
Cell & Tissue Engineering
Angela W. Xie, Nicholas A. Zacharias, Bernard Y. K. Binder, William L. Murphy
Summary: This study compared MSC aggregates generated via three different methods and found differences in their structure and immunomodulatory phenotype under resting conditions and in response to inflammatory stimulus. The methods with faster aggregation kinetics formed aggregates with higher cell packing density and reduced ECM synthesis.
STEM CELLS TRANSLATIONAL MEDICINE
(2021)
Article
Multidisciplinary Sciences
Hyun-Ji Park, Jessica R. Hoffman, Milton E. Brown, Sruti Bheri, Olga Brazhkina, Young Hoon Son, Michael E. Davis
Summary: Small extracellular vesicles (sEVs) play a critical role in cardiac cell therapy by delivering molecular cargo and mediating cellular signaling. Knocking down miR-192-5p and miR-432-5p in cardiac c-kit+ cell-derived sEVs enhances their therapeutic capabilities and improves cardiac function, reducing fibrosis and necrotic inflammatory responses. This study suggests that targeting deleterious miRNAs in sEVs could be a promising therapeutic strategy for chronic myocardial infarction.
Article
Pharmacology & Pharmacy
Ilenia Mastrolia, Andrea Giorgini, Alba Murgia, Pietro Loschi, Tiziana Petrachi, Valeria Rasini, Massimo Pinelli, Valentina Pinto, Francesca Lolli, Chiara Chiavelli, Giulia Grisendi, Maria Cristina Baschieri, Giorgio De Santis, Fabio Catani, Massimo Dominici, Elena Veronesi
Summary: In this study, a rabbit model was established to mimic human osteonecrosis of the femoral head (ONFH) and evaluate the autologous MSC-based treatment. Results showed that implantation of autologous BM-MSCs could restore the damaged tissue in rabbit femurs. Furthermore, the study found that osteonecrosis did not affect the behavior of MSCs.
Article
Cardiac & Cardiovascular Systems
Luis Henrique Wolff Gowdak, Isolmar Tadeu Schettert, Carlos Eduardo Rochitte, Leonardo P. de Carvalho, Marcelo Luiz Campos Vieira, Luis Alberto Oliveira Dallan, Sergio Almeida de Oliveira, Luiz Antonio Machado Cesar, Jose Oscar Reis Brito, Luiz Cesar Guarita-Souza, Antonio Carlos Campos de Carvalho, Jose Eduardo Krieger
Summary: In this study, we investigated the additional reduction of regional stress-induced myocardial ischemia (SIMI) in patients undergoing incomplete coronary artery bypass graft surgery (CABG) when autologous bone marrow-derived cells (BMC) were injected into non-revascularized segments. The results showed that BMC treatment led to additional improvement in regional SIMI after CABG, suggesting that BMC could be a potential adjuvant therapy for patients with coronary artery disease.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2023)
Article
Multidisciplinary Sciences
Mario Perez-Jimenez, Imre Derenyi, Gergely J. Szollosi
Summary: Almost all cancer types share the hallmarks of cancer and a similar tumor formation. In the case of chronic myeloid leukemia (CML), somatic evolution occurs in the context of healthy blood production, a hierarchical process of cell division. By introducing a general model of hierarchical cell division, we explain the particular progression of CML resulting from the structure of the hematopoietic system.
SCIENTIFIC REPORTS
(2023)
Article
Immunology
Miao Wei, Guowei Zhang, Zeyu Huang, Xuemeng Ding, Qing Sun, Yujian Zhang, Rongrong Zhu, Huaijin Guan, Min Ji
Summary: This study investigates the role of ATP-P2X(7)R in mediating microglial senescence and glaucoma progression. The results suggest that ATP-P2X(7)R induces microglial senescence by inhibiting the PINK1-mediated mitophagy pathway, leading to retinal ganglion cell damage. Specifically inhibiting ATP-P2X(7)R may be a fundamental approach for targeted therapy of RGC injury in microglial aging-related glaucoma.
JOURNAL OF NEUROINFLAMMATION
(2023)
Article
Oncology
Jennifer M. SanMiguel, Elizabeth Eudy, Matthew A. Loberg, Kira A. Young, Jayna J. Mistry, Kristina D. Mujica, Logan S. Schwartz, Timothy M. Stearns, Grant A. Challen, Jennifer J. Trowbridge
Summary: Clonal hematopoiesis resulting from enhanced fitness of mutant hematopoietic stem cells can lead to both favorable and unfavorable health outcomes. This study found that aging-induced TNF alpha signaling promotes the selective advantage of mutant HSCs and stimulates the production of mutant B lymphoid cells. The loss of TNFR1 abolishes the advantage of mutant HSCs, while the loss of TNFR2 results in overproduction of mutant myeloid cells without affecting HSC fitness.
Article
Cardiac & Cardiovascular Systems
Sebastiaan R. Piers, Alexander F. Androulakis, Kevin S. Yim, Nienke van Rein, Jeroen Venlet, Gijsbert F. Kapel, Hans-Marc Siebelink, Hildo J. Lamb, Suzanne C. Cannegieter, Sum-Che Man, Katja Zeppenfeld
Summary: This study found that spontaneous nonsustained ventricular tachycardia (NSVT) on Holter and VT inducibility during electrophysiology study can independently predict sustained ventricular arrhythmias (SVA) during follow-up in patients with nonischemic dilated cardiomyopathy (DCM). NSVT may serve as an initiator, while sustained VT inducibility indicates the presence of the substrate for SVA in DCM.
CIRCULATION-ARRHYTHMIA AND ELECTROPHYSIOLOGY
(2022)
Article
Hematology
Yassene Mohammed, Carolina E. Touw, Banne Nemeth, Raymond A. van Adrichem, Christoph H. Borchers, Frits R. Rosendaal, Bart J. van Vlijmen, Suzanne C. Cannegieter
Summary: This study explores the potential of using protein levels in blood plasma to predict venous thrombosis (VT) risk. The researchers identified specific proteins that were associated with VT development and used a logistic regression model to accurately predict VT risk.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2022)
Article
Hematology
Myrthe M. A. Toorop, Nienke van Rein, Melchior C. Nierman, Helga W. Vermaas, Menno Huisman, Felix J. M. van der Meer, Suzanne C. Cannegieter, Willem M. Lijfering
Summary: The study found that the inter-individual variability of DOAC concentrations was higher than intra-individual variability, and lower previous VKA dosage and creatinine clearance were associated with higher DOAC trough concentrations.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2022)
Article
Hematology
Cathrine Ramberg, Kristian Hindberg, Joseph S. Biedermann, Suzanne C. Cannegieter, Felix J. van der Meer, Omri Snir, Frank W. G. Leebeek, Marieke J. H. A. Kruip, John-Bjarne Hansen, Willem M. Lijfering
Summary: The study investigated the impact of rosuvastatin treatment on plasma PPL activity and extracellular vesicle (EV) levels. The results showed that rosuvastatin treatment led to a substantial decrease in plasma PPL activity.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2022)
Editorial Material
Hematology
Sufia N. Amini, Vivianne S. Nelson, Leendert Porcelijn, Tanja Netelenbos, Jaap Jan Zwaginga, Masja de Haas, Martin R. Schipperus, Rick Kapur
Summary: ITP is an autoimmune bleeding disorder characterized by platelet clearance in the spleen and liver. Thrombopoietin (TPO) levels remain low in ITP. The role of GPIb antibodies in relation to platelet hepatic sequestration and TPO levels in ITP remains unclear. This study found a positive association between GPIb antibodies and TPO levels as well as platelet hepatic sequestration in patients with severe thrombocytopenic ITP.
Article
Hematology
Astrid G. S. van Halteren, Jessica S. Suwandi, Sander Tuit, Jelske Borst, Sandra Laban, Roula Tsonaka, Ada Struijk, Anna-Sophia Wiekmeijer, Melissa van Pel, Bart O. Roep, Jaap Jan Zwaginga, Arjan C. Lankester, Koen Schepers, Maarten J. D. van Tol, Willem E. Fibbe
Summary: Acute graft-versus-host disease (aGVHD) is a potentially fatal complication of stem cell transplantation. This study used mass cytometry to analyze the immune cells in children with severe aGVHD and compared the results with control groups. The onset of aGVHD was associated with the appearance of specific myeloid and lymphoid cells in the blood and affected organs such as the skin and gastrointestinal tract. The findings highlight the importance of immune cell profiling for evaluating clinical response and managing severe aGVHD.
Article
Infectious Diseases
Arvind Gharbharan, Carlijn Jordans, Lisa Zwaginga, Grigorios Papageorgiou, Nan van Geloven, Peter van Wijngaarden, Jan den Hollander, Faiz Karim, Elena van Leeuwen-Segarceanu, Robert Soetekouw, Jolanda Lammers, Douwe Postma, Linda Kampschreur, Geert Groeneveld, Francis Swaneveld, C. Ellen van der Schoot, Hannelore Gotz, Bart Haagmans, Marion Koopmans, Susanne Bogers, Corine Geurtsvankessel, Jaap Jan Zwaginga, Casper Rokx, Bart Rijnders
Summary: This study aimed to determine the effectiveness of convalescent plasma therapy in high-risk outpatients with COVID-19. The results showed that convalescent plasma therapy did not improve the disease severity score.
CLINICAL MICROBIOLOGY AND INFECTION
(2023)
Article
Hematology
Fleur H. J. Kaptein, Milou A. M. Stals, Richard E. Evenhuis, Hans Gelderblom, Menno V. Huisman, Diederik S. A. Karis, Renee W. D. Noten, Suzanne C. Cannegieter, Frank M. Speetjens, Arjan J. Verschoor, Henri H. Versteeg, Michiel A. J. van de Sande, Frederikus A. Klok
Summary: Patients with osteosarcoma and Ewing sarcoma have a high risk of venous thromboembolism, especially in patients aged 18 and above. Catheter-related upper-extremity thrombosis is the most frequent presentation of venous thromboembolism. Major bleeding is common in both types of sarcoma.
THROMBOSIS RESEARCH
(2023)
Article
Hematology
Maaike Y. Kapteijn, Fleur H. J. Kaptein, Milou A. M. Stals, Eva E. Klaase, Ronald van Eijk, Dina Ruano, Sjoerd G. van Duinen, Suzanne C. Cannegieter, Martin J. B. Taphoorn, Linda Dirven, Johan A. F. Koekkoek, Frederikus A. Klok, Henri H. Versteeg, Jeroen T. Buijs, Ines Garcia-Ortiz
Summary: This study suggests a potential role for CDKN2A deletion in glioblastoma-related VTE, providing a potential predictive marker for this population.
THROMBOSIS RESEARCH
(2023)
Article
Immunology
Adam C. Levine, Yuriko Fukuta, Moises A. Huaman, Jiangda Ou, Barry R. Meisenberg, Bela Patel, James H. Paxton, Daniel F. Hanley, Bart J. A. Rijnders, Arvind Gharbharan, Casper Rokx, Jaap Jan Zwaginga, Andrea Alemany, Oriol Mitja, Dan Ouchi, Pere Millat-Martinez, Valerie Durkalski-Mauldin, Frederick K. Korley, Larry J. Dumont, Clifton W. Callaway, Romina Libster, Gonzalo Perez Marc, Diego Wappner, Ignacio Esteban, Fernando Polack, David J. Sullivan
Summary: COVID-19 convalescent plasma treatment can reduce the risk of hospitalization, especially when administered within 5 days of symptom onset and with higher antibody titers.
CLINICAL INFECTIOUS DISEASES
(2023)
Review
Physiology
R. P. B. Tonino, L. M. Zwaginga, M. R. Schipperus, J. J. Zwaginga
Summary: Abnormal hemoglobin concentration significantly affects a person's quality of life and physiology. Current tools for evaluating hemoglobin-related outcomes are lacking, leading to uncertainty in optimal hemoglobin levels and treatment targets. Reviews show that increasing hemoglobin improves patient-reported and physical outcomes, particularly at lower hemoglobin levels. However, there is a lack of high-quality evidence in this area.
FRONTIERS IN PHYSIOLOGY
(2023)
Article
Medicine, Research & Experimental
Laura F. Ouboter, Marieke C. Barnhoorn, Hein W. Verspaget, Leonie Plug, Emma S. Pool, Karoly Szuhai, Lukas J. A. C. Hawinkels, Melissa van Pel, Jaap Jan Zwaginga, Dave Roelen, Frits Koning, M. Fernanda Pascutti, Andrea E. van der Meulen-de Jong
Summary: A phase IIa clinical study evaluated the impact of local mesenchymal stromal cell (MSC) therapy on ulcerative proctitis (UP), and found it to be safe, feasible, and showed encouraging signs of clinical efficacy and modulation of local immune responses.
Article
Cardiac & Cardiovascular Systems
Judith Van Paassen, Alice De Graaf-Dijkstra, Anja H. Brunsveld-Reinders, Evert De Jonge, Robert J. M. Klautz, Roula Tsonaka, Jaap Jan Zwaginga, M. Sesmu Arbous
Summary: Acute lung injury after cardiac surgery is associated with an unfavorable outcome. Prior to surgery, cardiac surgery patients who developed lung injury showed higher platelet activatability and indications for higher neutrophil turnover. Further research is needed to determine the relationship between these factors and the development of lung injury after cardiac surgery.
INTERDISCIPLINARY CARDIOVASCULAR AND THORACIC SURGERY
(2023)
Article
Multidisciplinary Sciences
Pere Millat-Martinez, Arvind Gharbharan, Andrea Alemany, Casper Rokx, Corine Geurtsvankessel, Grigorios Papageourgiou, Nan van Geloven, Carlijn Jordans, Geert Groeneveld, Francis Swaneveld, Ellen van der Schoot, Marc Corbacho-Monne, Dan Ouchi, Francini Piccolo Ferreira, Pierre Malchair, Sebastian Videla, Vanesa Garcia Garcia, Anna Ruiz-Comellas, Anna Ramirez-Morros, Joana Rodriguez Codina, Rosa Amado Simon, Joan-Ramon Grifols, Julian Blanco, Ignacio Blanco, Jordi Ara, Quique Bassat, Bonaventura Clotet, Barbara Baro, Andrea Troxel, Jaap Jan Zwaginga, Oriol Mitja, Bart J. A. Rijnders
Summary: This study assessed whether convalescent plasma (CP) administered during the first week of symptoms reduced disease progression or risk of hospitalization in COVID-19 outpatients. The results showed that CP had no significant impact on disease severity scale and hospitalization or death, but may have some effect in patients with symptoms lasting <= 5 days. Additionally, CP did not shorten the time to full symptom resolution.
NATURE COMMUNICATIONS
(2022)
Meeting Abstract
Hematology
R. J. S. Anijs, Q. Chen, W. M. Lijfering, S. C. Cannegieter
THROMBOSIS RESEARCH
(2022)
