Article
Biochemistry & Molecular Biology
Xiang Zhao, Bethany N. Radford, Mark Ungrin, Wendy Dean, Myriam Hemberger
Summary: Normal developmental progression relies on close interactions between the embryonic and extraembryonic lineages in the pre- and peri-gastrulation stage conceptus. Trophoblast cells of the extraembryonic ectoderm play a role in delaying cardiomyocyte differentiation dynamics and prolonging pluripotency in embryonic stem cells (ESCs). These findings provide insights into the intricate crosstalk between different compartments of the early conceptus and have implications for synthetic embryo research.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Jose Manuel Inacio, Joao von Gilsa Lopes, Ana Mafalda Silva, Fernando Cristo, Sara Marques, Matthias E. Futschik, Jose Antonio Belo
Summary: Deciphering the clues of a regenerative mechanism for the mammalian adult heart, particularly focusing on the role of DAND5, could potentially lead to the development of more effective treatment options for heart failure. Further research on the transcriptional profiling sequencing and quantitative RT-PCR assays of DAND5 may provide insights into the generation and expansion of pluripotent stem cell-derived cardiomyocytes systems for clinical applications.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Yuting Fu, Fangyuan Liu, Shuo Cao, Jia Zhang, Huizhi Wang, Baojiang Wu, Yongli Song, Shuguang Duo, Xihe Li, Siqin Bao
Summary: This study reveals that Bdh2 deficiency has no impact on pluripotency and self-renewal of ESCs, but promotes endoderm differentiation and is essential for maintaining DNA methylation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Medicine, General & Internal
Xiuya Li, Fei Gao, Xiaochen Wang, Qianqian Liang, Aobing Bai, Zhuo Liu, Xinyun Chen, Ermin Li, Sifeng Chen, Chao Lu, Ruizhe Qian, Ning Sun, Ping Liang, Chen Xu
Summary: Knockout of the transcription factor E2A increased the proportion of nodal-like cells in hESC-derived CMs, indicating a potential role of E2A in enhancing the efficiency of generating sinoatrial node-like cardiomyocytes from human pluripotent stem cells in the future.
Article
Cell Biology
Seung-Cheol Choi, Ha-Rim Seo, Long-Hui Cui, Myeong-Hwa Song, Ji-Min Noh, Kyung-Seob Kim, Ji-Hyun Choi, Jong-Ho Kim, Chi-Yeon Park, Hyung Joon Joo, Soon Jun Hong, Tae Hee Ko, Jong-Il Choi, Hyo Jin Kim, Jong-Hoon Kim, Se-Hwan Paek, Ji-Na Park, Dong-Hyung Kim, Yongjun Jang, Yongdoo Park, Do-Sun Lim
Summary: The study demonstrates that FGF4 and ascorbic acid can induce differentiation of human embryonic stem cell-cardiogenic mesoderm cells into mature cardiomyocytes, providing a model for adult-onset cardiac disease and drug discovery. These mature cardiomyocytes release acute myocardial infarction biomarkers in response to hypoxic injury, with potential for diagnosis and prognosis in coronary artery diseases based on transcriptome analyses.
Article
Biochemistry & Molecular Biology
Yan Li, Zhiyu Xia, Haihong Yin, Youran Dai, Feixue Li, Jianming Chen, Mengsheng Qiu, Huarong Huang
Summary: A simple and efficient method for inducing mouse embryonic stem cells into primitive endoderm cells was reported in this study, and it was found that the Wnt signaling pathway played a critical role in this process.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Cell Biology
Chika Ogura, Shoko Nishihara
Summary: This study demonstrated the important role of DS in maintaining the undifferentiated state of mESCs and influencing their differentiation direction and Wnt signaling pathway. Knockdown or overexpression experiments targeting the D4ST1 gene revealed the mechanism of DS in mESCs.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Edward J. Calabrese
Summary: This paper provides a thorough assessment of hormetic dose responses in embryonic stem cells, suggesting that this response may serve as a fundamental and highly conserved evolutionary strategy.
CHEMICO-BIOLOGICAL INTERACTIONS
(2022)
Article
Chemistry, Physical
Francesca Amantea, Gabriella Antignani, Giulio Pota, Emanuela Cascone, Silvia Parisi, Michela Alfe, Valentina Gargiulo, Giuseppina Luciani, Alessandro Pezzella, Gerardino D'Errico, Roberto Di Capua, Giuseppe Vitiello
Summary: The design and fabrication of platforms reproducing the microenvironment in which the cells can properly acquire a specific fate is crucial for stem cell-based nanomedicine. In this study, a bio-interface composed of hybrid eumelanin/graphene-like/titanium dioxide (EU/GL/TiO2) nanostructures was prepared to support the survival and growth of stem cells and stimulate their differentiation. In vitro tests demonstrated that the prepared platform efficiently supports the differentiation of stem cells into beating cardiomyocytes and neuronal cells.
APPLIED SURFACE SCIENCE
(2023)
Review
Biology
Afshin Zare, Aria Salehpour, Arezoo Khoradmehr, Shabnam Bakhshalizadeh, Vahid Najafzadeh, Sahar Almasi-Turk, Mahdi Mahdipour, Reza Shirazi, Amin Tamadon
Summary: More research is being conducted on using stem cell lines to develop into cardiomyocytes for myocardial cell treatments. Embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) have shown promise in treating various cardiac illnesses. This study explores the differentiation of these cells into cardiomyocytes from an epigenetic perspective, including histone acetylation, methylation, DNA alterations, RNA methylation, and mitochondrial mutations.
Article
Engineering, Biomedical
Lakshmi Kavitha Sthanam, Tanusri Roy, Sejal Patwardhan, Avi Shukla, Shipra Sharma, Pradip Shinde, Hanuman Tulasiram Kale, P. Chandra Shekar, Kiran Kondabagil, Shamik Sen
Summary: Stem cell differentiation is influenced by the interaction between cells and their extracellular matrix, with matrix metalloproteinases (MMPs) playing a role in this process. Differentiation of mouse embryonic stem cells (mESCs) on varying matrices led to changes in MMP expression and activity, affecting integrin expression, mechanotransduction pathways, and germ layer-specific markers. Inhibition of MMP activity altered the germ layer commitment of differentiating mESCs, demonstrating the importance of MMPs in regulating stem cell differentiation.
Article
Cell Biology
Masahiro Otsu, Zubair Ahmed, Daniel Fulton
Summary: A simple method was developed to generate immature oligodendrocyte lineage cells from mouse ESC-derived neural stem cells, which showed potential for differentiation into mature OPC under specific conditions. Interestingly, these cells retained multipotentiality and could differentiate into neurons or astrocytes. However, their efficiency in myelinating axons was lower compared to primary OPC.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Jingsheng Li, Chunhong Dai, Wenyan Xie, Heyao Zhang, Xin Huang, Constantinos Chronis, Ying Ye, Wensheng Zhang
Summary: This study developed a one-step strategy to efficiently target and suppress endogenous pluripotent genes in mouse ESCs and replace their expression with AID-fused transgenes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Pharmacology & Pharmacy
Michelle Vanessa Kamga Kapchoup, Juergen Hescheler, Filomain Nguemo
Summary: Hydroxychloroquine (HDQ) has been widely studied for its potential use in the treatment and prevention of COVID-19. This antimalarial drug has shown beneficial effects on stem cell proliferation and cardiomyocyte activity, but it can also be toxic at high concentrations. Identifying risk factors and understanding the cardiotoxic profile of HDQ is challenging.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Multidisciplinary Sciences
Shao-Hui Pan, Ning Zhao, Xiang Feng, Ying Jie, Zi-Bing Jin
Summary: The study developed a stepwise strategy to generate chemically induced neural crest cells (ciNCCs) and chemically induced corneal endothelial cells (ciCECs) from mouse fibroblasts using defined small molecules. The resulting ciCECs exhibited consistent gene expression profiles and self-renewal capacity to primary CECs, and could be cultured for as long as 30 passages while retaining their features. Transplantation of these ciCECs into an animal model reversed corneal opacity, providing an alternative cell source for regeneration of corneal endothelia and tissues derived from neural crest.
Article
Cell Biology
Mohamed M. Bekhite, Veronika Mueller, Sebastian H. Troeger, Joerg P. Mueller, Hans-Reiner Figulla, Heinrich Sauer, Maria Wartenberg
CELL AND TISSUE RESEARCH
(2016)
Article
Biochemistry & Molecular Biology
Fatemeh Sharifpanah, Sepali De Silva, Mohamed M. Bekhite, Jorge Hurtado-Oliveros, Klaus T. Preissner, Maria Wartenberg, Heinrich Sauer
FREE RADICAL BIOLOGY AND MEDICINE
(2015)
Article
Biochemistry & Molecular Biology
Safoura Mazrouei, Fatemeh Sharifpanah, Mohamed M. Bekhite, Hans-Reiner Figulla, Heinrich Sauer, Maria Wartenberg
PURINERGIC SIGNALLING
(2015)
Article
Reproductive Biology
Mohamed M. Bekhite, Andreas Finkensieper, Fouad A. Abou-Zaid, Ibrahim K. El-Shourbagy, Nabil K. El-Fiky, Khaled M. Omar, Heinrich Sauer, Maria Wartenberg
REPRODUCTIVE TOXICOLOGY
(2016)
Article
Cell Biology
Fatemeh Sharifpanah, Fatjon Saliu, Mohamed M. Bekhite, Maria Wartenberg, Heinrich Sauer
CELL AND TISSUE RESEARCH
(2014)
Article
Genetics & Heredity
Julia Falk, Magdalena Rohde, Mohamed M. Bekhite, Sophie Neugebauer, Peter Hemmerich, Michael Kiehntopf, Thomas Deufel, Christian A. Huebner, Christian Beetz
Review
Cell & Tissue Engineering
Mohamed M. Bekhite, Andreas Finkensieper, Jennifer Rebhan, Stephanie Huse, Stefan Schultze-Mosgau, Hans-Reiner Figulla, Heinrich Sauer, Maria Wartenberg
STEM CELLS AND DEVELOPMENT
(2014)
Article
Cardiac & Cardiovascular Systems
Mohamed M. Bekhite, Andres Gonzalez Delgado, Florian Menz, Tom Kretzschmar, Jasmine M. F. Wu, Tarek Bekfani, Sandor Nietzsche, Maria Wartenberg, Martin Westermann, Boris Greber, P. Christian Schulze
BASIC RESEARCH IN CARDIOLOGY
(2020)
Article
Cardiac & Cardiovascular Systems
Tarek Bekfani, Mohamed Bekhite Elsaied, Steffen Derlien, Jenny Nisser, Martin Westermann, Sandor Nietzsche, Ali Hamadanchi, Elisabeth Frob, Julian Westphal, Daniela Haase, Tom Kretzschmar, Peter Schlattmann, Ulrich C. Smolenski, Michael Lichtenauer, Bernhard Wernly, Peter Jirak, Gabriele Lehmann, Sven Mobius-Winkler, P. Christian Schulze
CIRCULATION-HEART FAILURE
(2020)
Article
Biochemistry & Molecular Biology
Mohamed Bekhite, Andres Gonzalez-Delgado, Sascha Huebner, Pellumb Haxhikadrija, Tom Kretzschmar, Tina Mueller, Jasmine M. F. Wu, Tarek Bekfani, Marcus Franz, Maria Wartenberg, Markus Graeler, Boris Greber, P. Christian Schulze
Summary: Ceramide accumulation in cardiomyocytes contributes to insulin resistance, oxidative stress, increased auto/mitophagy, and mitochondrial dysfunction in the setting of lipotoxic cardiomyopathy, highlighting the importance of targeting the de novo ceramide synthesis pathway as a potential therapeutic strategy.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Tom Kretzschmar, Mohamed M. Bekhite, Jasmine M. F. Wu, Daniela Haase, Martin Foerster, Tina Mueller, Sandor Nietzsche, Martin Westermann, Marcus Franz, Markus H. Graeler, P. Christian Schulze
Summary: The study reveals that Doxorubicin increases ceramide levels, leading to cell death and fibrosis, and that inhibiting ceramide synthesis can partially reverse the associated damage.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Cell Biology
Mohamed M. Bekhite, P. Christian Schulze
Summary: Understanding human heart disease and drug responses is limited by species differences, but the discovery of human-induced pluripotent stem cells offers a new potential. Reprogramming and genome editing technologies allow for a broad spectrum of human heart diseases to be generated, providing opportunities to elucidate gene function and mechanisms.
Article
Cell Biology
Tarek Bekfani, Mohamed Bekhite, Sophie Neugebauer, Steffen Derlien, Ali Hamadanchi, Jenny Nisser, Marion S. Hilse, Daniela Haase, Tom Kretzschmar, Mei-Fang Wu, Michael Lichtenauer, Michael Kiehntopf, Stephan von Haehling, Peter Schlattmann, Gabriele Lehmann, Marcus Franz, Sven Moebius-Winkler, Christian Schulze
Summary: This study aimed to investigate the metabolic and structural changes in the skeletal muscle of patients with heart failure and reduced muscle endurance (RME). The study found that Kynurenine may serve as a marker for RME and that there are different metabolomic profiles between patients with heart failure with preserved and reduced ejection fraction.
Meeting Abstract
Cardiac & Cardiovascular Systems
P. Haxhikadrija, M. Bekhite, T. Kretzschmar, J. Wu, A. Maloku, T. Mueller, M. Graeler, M. Franz, C. Schulze
EUROPEAN HEART JOURNAL
(2021)
Article
Anatomy & Morphology
Fatemeh Sharifpanah, Matthias Reinhardt, Johanna Schoenleben, Claudia Meyer, Madeleine Richter, Matthias Schnabelrauch, Claudia Rode, Annika Wartenberg, Mohamed Bekhite, Heinrich Sauer, Maria Wartenberg
CELLS TISSUES ORGANS
(2017)