Article
Medicine, General & Internal
Lisa Takahashi, Tomoaki Ishigami, Hirofumi Tomiyama, Yuko Kato, Hiroyuki Kikuchi, Koichiro Tasaki, Jun Yamashita, Shigeru Inoue, Masataka Taguri, Toshitaka Nagao, Taishiro Chikamori, Yoshihiro Ishikawa, Utako Yokoyama
Summary: Research has shown that myosin-11 may serve as a biomarker for detecting spatial expansion of atherosclerotic regions, with circulating levels associated with the presence of multiple atherosclerotic areas. Additionally, myosin-11 is predominantly expressed in the medial layer of human atherosclerotic lesions.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Multidisciplinary Sciences
Sarah M. Heissler, Amandeep S. Arora, Neil Billington, James R. Sellers, Krishna Chinthalapudi
Summary: Using single-particle cryo-electron microscopy, we determined the near-atomic resolution structure of smooth muscle myosin-2 in the autoinhibited state, revealing the molecular mechanisms behind its regulation. Our study also identified a previously unknown conformational state in myosin-2 and explained the autoinhibition of its enzyme function in 10S. This research provides insights into the formation, stabilization, and relief of 10S through phosphorylation of the regulatory light chain.
Article
Hematology
Rebecca A. Deaton, Gamze Bulut, Vlad Serbulea, Anita Salamon, Laura S. Shankman, Anh Tram Nguyen, Gary K. Owens
Summary: This study introduces a new Myh11-CreER(T2)-RAD mouse model that can be used for lineage tracing and gene knockout studies of smooth muscle cells (SMCs) in both male and female mice. The innovation of this model is that the Myh11 transgene is inserted into mouse chromosome 2, allowing for study in female mice. Flow cytometric and immunofluorescence analyses of mouse samples confirmed that this model can label vascular and visceral SMCs and pericytes.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2023)
Article
Fisheries
Qingyue Chen, Wanmei Yu, Ruijuan Hao, Jingmiao Yang, Chuangye Yang, Yuewen Deng, Yongshan Liao
Summary: This study investigated the MYH gene in Pinctada fucata martensii and found its important role in muscle growth and contraction. The research also discovered the expression pattern of PmMYH in different tissues and developmental stages, as well as identified growth-related SNP markers.
AQUACULTURE RESEARCH
(2022)
Review
Physiology
Joseph F. Y. Hoh
Summary: This review examines the kinetic properties and distribution of MyHC in extraocular muscles, as well as their regulation and function. Different MyHC are regulated by different mechanisms in neurogenic and myogenic processes and express differently during development. Lastly, the combination of different MyHC and speeds play a role in accomplishing eye movement tasks.
Article
Medicine, Research & Experimental
Kimberly A. Queisser, Rebecca A. Mellema, Elizabeth A. Middleton, Irina Portier, Bhanu Kanth Manne, Frederik Denorme, Ellen J. Beswick, Matthew T. Rondina, Robert A. Campbell, Aaron C. Petrey
Summary: Vascular injury in COVID-19 patients is linked to increased levels of glycosaminoglycans, particularly hyaluronan fragments and hyaluronidase activity. This damage to the glycocalyx is associated with disease severity and elevated levels of inflammatory cytokines. Pulmonary microvascular endothelial cells exposed to the COVID-19 milieu exhibit dysregulated HA biosynthesis and degradation, leading to the release of pathological HA fragments into circulation.
Article
Cardiac & Cardiovascular Systems
Antonija Sakic, Chiraz Chaabane, Noona Ambartsumian, Jorg Klingelhofer, Sylvain Lemeille, Brenda R. Kwak, Mariam Grigorian, Marie-Luce Bochaton-Piallat
Summary: This study investigates the role of extracellular S100A4 in the phenotypic transition of smooth muscle cells during atherosclerosis. The findings suggest that neutralization of extracellular S100A4 promotes the stabilization of atherosclerotic plaques and could potentially be a new target to influence plaque evolution.
CARDIOVASCULAR RESEARCH
(2022)
Article
Clinical Neurology
C. A. Sewrya, L. Feng, D. Chambers, E. Matthews, R. Phadke
Summary: The study found that embryonic myosin heavy chains are down-regulated at or soon after birth, while fetal myosin heavy chains are down-regulated at 4-6 months. The co-expression of embryonic and fetal myosin heavy chains in dystrophic muscle may suggest delays in maturation or underlying abnormalities. Embryonic myosin heavy chains are a useful marker for regeneration, but other factors like denervation and stress must also be considered.
NEUROMUSCULAR DISORDERS
(2021)
Article
Nutrition & Dietetics
Xiaoyu Wang, Guoqing Liu, Shouqi Xie, Lei Pan, Qingsong Tan
Summary: Appropriate dietary protein levels improved the growth performance and flesh quality of grass carp by regulating muscle antioxidant capacity, gene expression, and fat metabolism-related signaling molecules.
FRONTIERS IN NUTRITION
(2022)
Article
Biochemistry & Molecular Biology
Wioleta Ciempiel, Magdalena Czemierska, Monika Szymanska-Chargot, Artur Zdunek, Dariusz Wiacek, Anna Jarosz-Wilkolazka, Izabela Krzeminska
Summary: This study investigated the potential of extracellular polymeric substances (EPS) produced by Parachlorella kessleri and Chlorella vulgaris in removing lead and cadmium. EPS from both species contained carbohydrates as the dominant components. C. vulgaris EPS had higher contents of reducing sugars, uronic acids, and amino acids compared to P. kessleri EPS. Monosaccharide composition analysis showed the presence of rhamnose, mannose, and galactose in the EPS from both species. ICP-OES analyses demonstrated that C. vulgaris EPS had higher sorption capacity than P. kessleri EPS. The study also found that carboxyl, hydroxyl, and carbonyl groups of EPS played a key role in interacting with metal ions. Overall, C. vulgaris EPS showed promising potential as a biosorbent in bioremediation processes.
Review
Physiology
Joseph Foon Yoong Hoh
Summary: The kinetics of myosin control the speed and power of muscle contraction. Mammalian skeletal muscles express twelve kinetically different myosin heavy chain (MyHC) genes, providing a wide range of muscle speeds to meet various functional demands. This review provides an overview of the historical and current views on how cell lineage, neural impulse patterns, and thyroid hormone influence MyHC gene expression in limb muscles during development and adult life, and the molecular mechanisms involved.
JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMS AND ENVIRONMENTAL PHYSIOLOGY
(2023)
Article
Multidisciplinary Sciences
Keita Negishi, Kenichi Aizawa, Takayuki Shindo, Toru Suzuki, Takayuki Sakurai, Yuichiro Saito, Takuya Miyakawa, Masaru Tanokura, Yosky Kataoka, Mitsuyo Maeda, Shota Tomida, Hiroyuki Morita, Norifumi Takeda, Issei Komuro, Kazuomi Kario, Ryozo Nagai, Yasushi Imai
Summary: Pathogenic variants in the Myh11 gene cause familial thoracic aortic aneurysms and dissections (FTAAD). This study established a mouse model carrying the Myh11 K1256del variant, which was found in two FTAAD families. The Myh11(K/K) aorta showed increased wall thickness and ultrastructural abnormalities, including weakened cell adhesion. Interestingly, the Myh11(K/+) mice developed aortic dissections and intramural hematomas when stimulated with angiotensin II. Mechanistically, the downregulation of integrin subunit alpha2 (Itga2) was observed in the Myh11(K/K) aorta and smooth muscle cells differentiated from Myh11(K/K) induced pluripotent stem cells. The contractility of the Myh11(K/K) aorta in response to phenylephrine was also reduced. These findings suggest that the suboptimal cell adhesion caused by Itga2 downregulation leads to a defect in aortic contraction, potentially contributing to the development of aortic dissections.
SCIENTIFIC REPORTS
(2022)
Article
Immunology
Yuanyuan Li, Ziyao Guo, Guangli Zhang, Xiaoyin Tian, Qinyuan Li, Zhengxiu Luo
Summary: The study revealed that neonatal S. pneumoniae pneumonia promotes airway smooth muscle myosin heavy chain (SMMHC) overexpression and airway hyperresponsiveness (AHR) through the HMGB1/TLR4/ERK pathway.
IMMUNOLOGY LETTERS
(2021)
Article
Biochemistry & Molecular Biology
O. P. Gerzen, Iu. K. Potoskueva, Yu. V. Permyakova, A. V. Grebenschchikova, I. S. Selezneva, L. V. Nikitina
Summary: Using our modified one-dimensional SDS-PAGE method, we successfully separated myosin heavy chains from different chambers of rat heart. The modification includes changes in gel composition and buffer, as well as the use of constant current. These changes allow for a safer and faster separation of cardiac myosin heavy chains using fewer reagents.
JOURNAL OF EVOLUTIONARY BIOCHEMISTRY AND PHYSIOLOGY
(2022)
Article
Biophysics
Natalia A. Koubassova, Andrey K. Tsaturyan, Sergey Y. Bershitsky, Michael A. Ferenczi, Raul Padron, Roger Craig
Summary: Through electron microscopy and X-ray modeling, it is found that myosin heads in relaxed muscles interact with each other and with the myosin tail. However, a recent X-ray modeling study reaches a contrary conclusion, possibly due to neglecting the radial position of the myosin heads in thick filaments. When the myosin heads are placed at the appropriate radius, the X-ray diffraction pattern of intact muscle can be explained.
BIOPHYSICAL JOURNAL
(2022)
Letter
Cardiac & Cardiovascular Systems
A. P. Patrianakos, M. Touloupaki, M. Marketou, F. I. Parthenakis
HELLENIC JOURNAL OF CARDIOLOGY
(2019)
Article
Surgery
George Papadopoulos, Nikolaos Kontopodis, Maria E. Marketou, Alexandros Patrianakos, Stella Lioudaki, Fragiskos I. Parthenakis, Christos V. Ioannou
ANNALS OF VASCULAR SURGERY
(2019)
Article
Immunology
M. Kavousanaki, M. Tzagournissakis, I. Zaganas, K. G. Stylianou, A. P. Patrianakos, M. K. Tsilimbaris, A. Mantaka, D. N. Samonakis
TRANSPLANTATION PROCEEDINGS
(2019)
Article
Cardiac & Cardiovascular Systems
Mitja Lainscak, Ivan Milinkovic, Marija Polovina, Marisa G. Crespo-Leiro, Lars H. Lund, Stefan D. Anker, Cecile Laroche, Roberto Ferrari, Andrew J. S. Coats, Theresa McDonagh, Gerasimos Filippatos, Aldo R. Maggioni, Massimo F. Piepoli, Giuseppe M. C. Rosano, Frank Ruschitzka, Dragan Simic, Milika Asanin, Jean-Christophe Eicher, Mehmet B. Yilmaz, Petar M. Seferovic
EUROPEAN JOURNAL OF HEART FAILURE
(2020)
Article
Cardiac & Cardiovascular Systems
Eleni S. Nakou, Maria E. Marketou, Alexandros Patrianakos, Alexandros Protonotarios, Panos E. Vardas, Fragiskos Parthenakis
HELLENIC JOURNAL OF CARDIOLOGY
(2020)
Letter
Cardiac & Cardiovascular Systems
Patrick Rossignol, Mitja Lainscak, Maria G. Crespo-Leiro, Cecile Laroche, Massimo F. Piepoli, Gerasimos Filippatos, Giuseppe M. C. Rosano, Gianluigi Savarese, Stefan D. Anker, Petar M. Seferovic, Frank Ruschitzka, Andrew J. S. Coats, Alexandre Mebazaa, Theresa McDonagh, Ana Sahuquillo, Maria Penco, Aldo P. Maggioni, Lars H. Lund
EUROPEAN JOURNAL OF HEART FAILURE
(2020)
Letter
Cardiac & Cardiovascular Systems
Kalliopi Keramida, Christos Maniotis, George Makavos, Constantinos H. Papadopoulos, Alexandros Stefanidis, Anastasios Theodossis Georgilas, Demetrios J. Beldekos, Nikolaos Kouris
HELLENIC JOURNAL OF CARDIOLOGY
(2021)
Article
Cardiac & Cardiovascular Systems
Stamatis Adamopoulos, Dimitrios Miliopoulos, Apostolis Karavidas, Maria Nikolaou, George Lazaros, Angeliki Gkouziouta, Athanassios Manginas, George Sevastos, Haralambos Karvounis, Theodoros D. Karamitsos, George Hahalis, Marianna Leopoulou, Konstantinos Grigoriou, Despoina Balta, Catherine C. Avgeropoulou, Alexandros Kasiakogias, Ioannis Mantas, Nikolaos Daskalopoulos, Dimitrios Varvarousis, Fragiskos I. Parthenakis, Alexandros P. Patrianakos, Sotirios Patsilinakos, Stavros Karanikas, Stavros V. Konstantinides, Dimitrios N. Tziakas, Nikolaos Kouvelas, Paraskevi Ntoliou, Athanasios J. Manolis, Pavlos Tsinivizov, Efstathios K. Iliodromitis, Agathi-Rosa Vrettou, Stavros N. Kakouros, Alexandros Douras, Nikoleta Mpaka, Pantelis Makridis, Eleni Karapatsoudi, Neofytos Papoulidis, Antonios Sideris, John T. Parissis, Filippos Triposkiadis, Athanasios Trikas, Gerasimos Filippatos
Article
Surgery
Maria Marketou, George Papadopoulos, Nikolaos Kontopodis, Alexandros Patrianakos, Eleni Nakou, Spyros Maragkoudakis, Gregory Chlouverakis, Stavros Stratakis, Emmanouel Tavlas, Fragiskos Parthenakis, Christos V. Ioannou
Summary: Aortic reconstruction in patients with abdominal aortic aneurysm (AAA) using currently available grafts and endografts was found to increase pulse wave velocity and reduce cardiac systolic function. Further monitoring and studies are needed to assess the long-term cardiovascular complications and symptoms associated with this repair.
JOURNAL OF ENDOVASCULAR THERAPY
(2021)
Letter
Cardiac & Cardiovascular Systems
Joanna E. Kontaraki, Maria E. Marketou, George E. Kochiadakis, Alexandros Patrianakos, Spyros Maragkoudakis, Anthi Plevritaki, Sofia Papadaki, Afroditi Alevizaki, Ourania Theodosaki, Fragiskos I. Parthenakis
HELLENIC JOURNAL OF CARDIOLOGY
(2021)
Article
Peripheral Vascular Disease
Eleni Angelaki, Maria E. Marketou, Georgios D. Barmparis, Alexandros Patrianakos, Panos E. Vardas, Fragiskos Parthenakis, Giorgos P. Tsironis
Summary: Cardiac remodeling, an important aspect of CVD progression, was detected using machine learning techniques even in patients without established CVD. Features such as hypertension, age, BMI, QRS-T angle, and QTc duration were identified as important factors affecting cardiac geometry. The ML algorithm achieved high accuracy in distinguishing between different cardiac geometry categories, showing promise for early detection in patients without established CVD.
JOURNAL OF CLINICAL HYPERTENSION
(2021)
Review
Cardiac & Cardiovascular Systems
Eva D. Papadimitraki, Alexandros Patrianakos, Antonios Pitsis, Maria Marketou, Angeliki Zacharaki, Fragiskos Parthenakis
Summary: Mitral commissural prolapse or flail poses challenges in diagnosis and treatment, often requiring 3D imaging for accurate diagnosis and surgical planning. Various surgical techniques have shown good results in treating this condition.
ECHOCARDIOGRAPHY-A JOURNAL OF CARDIOVASCULAR ULTRASOUND AND ALLIED TECHNIQUES
(2021)
Article
Cardiac & Cardiovascular Systems
Maria E. Marketou, Joanna Kontaraki, Alexandros Patrianakos, Emmanuel Kanoupakis, Anthoula Plevritaki, Hercules Mavrakis, Eleutherios Kallergis, Emmanuel Koutalas, Ioannis Anastasiou, Gregory Chlouverakis, Fragiskos Parthenakis
Summary: This study evaluated the gene expression levels of myocardin in patients with non-ischemic dilated cardiomyopathy (NIDCM) and found that lower levels of myocardin were associated with higher mortality rates, particularly from congestive heart failure. These findings suggest that myocardin expression in peripheral blood cells may serve as a prognostic biomarker for the long-term outcome of patients with NIDCM, offering new prospects in risk stratification for these patients.
Article
Genetics & Heredity
Maria Marketou, Joanna Kontaraki, Alexandros Patrianakos, George Kochiadakis, Ioannis Anastasiou, Konstantinos Fragkiadakis, Anthoula Plevritaki, Sofia Thalia Papadaki, Gregory Chlouverakis, Fragiskos Parthenakis
Summary: The study found that the expression levels of miR-21-5p and miR-1-3p are elevated in acute viral myocarditis, and are correlated with myocardial damage and indicative of left ventricular dysfunction in these patients.
Article
Cardiac & Cardiovascular Systems
Maria E. Marketou, Ilias Zareas, Emmanuel Kanoupakis, Alexandros Patrianakos, Fragiskos Parthenakis
Summary: Two brothers with BrS were found to have the same mutation in the ankyrin-B gene, which is associated with stability of plasma membrane proteins in voltage-gated ion channels. This finding lays the groundwork for further investigation into the relationship between this mutation and BrS. Additionally, the timing of presentation raises concerns about the association between myocarditis or beta-blockers and the manifestation of BrS ECG.
EUROPEAN HEART JOURNAL-CASE REPORTS
(2021)
