Review
Food Science & Technology
Shuangshuang Zhang, Muhammad Bilal, Jakub Zdarta, Jiandong Cui, Ashok Kumar, Marcelo Franco, Luiz Fernando Romanholo Ferreira, Hafiz M. N. Iqbal
Summary: Pectinases are emerging enzymes in the biotechnology industry, with diverse industrial applications. Enzyme immobilization has become a promising approach to address challenges such as low operational stability and recyclability, leading to improved enzyme performance and commercial feasibility. Immobilization of pectinases using polymers and nanostructured materials has shown to enhance their thermal stability and catalytic characteristics for industrial exploitability.
FOOD RESEARCH INTERNATIONAL
(2021)
Article
Engineering, Environmental
Jantiya Isanapong, Peerapong Pornwongthong
Summary: The study found that immobilized laccase on zinc oxide nanostructure showed higher efficiency in TBA biodegradation compared to suspended enzyme, with potential for repeated use and cost reduction.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Review
Biotechnology & Applied Microbiology
Karen Cristina Collograi, Aline Carvalho da Costa, Jaciane Lutz Ienczak
Summary: This article summarizes the state of the art regarding strategies to improve propionic acid (PA) production by fermentation with the Propionibacterium genus. Strategies associated with environmental fermentation conditions and nutrition requirements are discussed, and the advantages and disadvantages of various strategies proposed to improve process performance are evaluated.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Karen Rodriguez-Nunez, Claudia Bernal, Ronny Martinez
Summary: The study introduces a novel enzyme improvement platform, Immobilized Biocatalyst Engineering (IBE), which integrates protein engineering (PE) and enzyme immobilization (EI) to simultaneously enhance biocatalysts. The IBE approach allows for the evaluation of thousands of variants in a short time, leading to the discovery of highly stable and active heterogeneous biocatalysts that show significant improvement over traditional methods. This innovative approach increases the probability of finding suitable biocatalysts for highly demanding processes.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Chemistry, Physical
Christopher K. Prier, Karla Camacho Soto, Jacob H. Forstater, Nadine Kuhl, Jeffrey T. Kuethe, Wai Ling Cheung-Lee, Michael J. Di Maso, Claire M. Eberle, Shane T. Grosser, Hsing- Ho, Erik Hoyt, Anne Maguire, Kevin M. Maloney, Amanda Makarewicz, Jonathan P. McMullen, Jeffrey C. Moore, Grant S. Murphy, Karthik Narsimhan, Weilan Pan, Nelo R. Rivera, Anumita Saha-Shah, David A. Thaisrivongs, Deeptak Verma, Adeya Wyatt, Daniel Zewge
Summary: Enzymes immobilized on solid supports in organic solvents have been used to catalyze a biocatalytic transaminase reaction, resulting in efficient and selective chemical synthesis. The advantages of enzyme immobilization include improved reaction efficiency, enhanced enzyme stability, reaction in non-aqueous media, and simplified product separation. By combining directed enzyme evolution with optimal reaction solvent and solid support for immobilization, the transaminase reaction of Cyrene (2) was successfully carried out, producing the amine intermediate 3a en route to the BTK inhibitor nemtabrutinib as a crystalline salt with dr > 50:1.
Review
Biotechnology & Applied Microbiology
Peng-Wei Huang, Ling-Ru Wang, Shan-Shan Geng, Chao Ye, Xiao-Man Sun, He Huang
Summary: Terpenoids, a large class of compounds in nature, are important in food and pharmaceutical fields, and microorganisms, especially microalgae, are promising sources for terpenoid production. Genetic engineering and fermentation strategies play vital roles in increasing terpenoid yields, while solvent extraction and supercritical fluid extraction are common downstream processes in industrial production.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Review
Environmental Sciences
Kondapalli Vamsi Krishna, Natarajan Bharathi, Shon George Shiju, Kuppusamy Alagesan Paari, Alok Malaviya
Summary: This paper discusses the sustainable production of biobutanol as a green fuel alternative to gasoline. Key issues include developing high-yield biobutanol strains, finding renewable biomass for large-scale production, and addressing limitations in traditional fermentation processes to improve biobutanol synthesis efficiency.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Review
Biochemical Research Methods
Dexun Fan, Huayang Tang, Xiaorong Yang, Fengguang Zhao, Shuangyan Han
Summary: This review provides an overview of the strategies for statin production via microbial cell factories, including traditional fermentation culture and modern synthetic biology manufacture. It discusses the complex fermentation parameters and process control technology for submerged fermentation and solid-state fermentation in detail. The potential use of recoverable agricultural wastes/biomass as a fermentation substrate in solid-state fermentation for statin production is emphasized. Additionally, metabolic engineering strategies for constructing robust engineering strains and directed evolution are also discussed. The review highlights the potential and challenges of using microbial cell factories for statin production and aims to promote greener production modes for statins.
BIOTECHNOLOGY JOURNAL
(2023)
Review
Biotechnology & Applied Microbiology
Zhenzhen Zhang, Zitong Zhao, Kunlun Huang, Zhihong Liang
Summary: Enzymes have promising applications in various industries due to their high efficiency, specificity, and environmentally friendly properties. However, their application range is limited due to the complexity of raw materials. This review discusses strategies for obtaining acid-resistant enzymes and their industrial applications.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Computer Science, Software Engineering
Giovani Guizzo, Federica Sarro, Jens Krinke, Silvia R. Vergilio
Summary: In this paper, a novel multi-objective evolutionary hyper-heuristic approach called Sentinel is proposed to automatically generate optimal cost reduction strategies for new software under test (SUT). Thorough empirical study involving 40 releases of 10 open-source real-world software systems shows that strategies generated by Sentinel outperform baseline strategies in 95 percent of the cases and achieve statistically significantly better results than state-of-the-art strategies in 88 percent of the cases.
IEEE TRANSACTIONS ON SOFTWARE ENGINEERING
(2022)
Article
Agricultural Engineering
Rong Fan, Josephine Dresler, Dennis Tissen, Linxuan Wen, Peter Czermak
Summary: FOS, a prebiotic sugar substitute produced from sucrose using FTases, was successfully produced without enzymes by immobilizing FTase on resin carriers, increasing enzyme selectivity. A hybrid process combining fed-batch fermentation with a probiotic bacterium and FTase conversion of residual sucrose depleted monosaccharides, raising FOS concentration to 130-170 g.L-1, and increasing overall purity to 92.1%.
BIORESOURCE TECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Karen Rodriguez-Nunez, Fernando Lopez-Gallego, Ronny Martinez, Claudia Bernal
Summary: Biocatalysis has the potential to enhance bioprocesses by identifying or enhancing enzymes that can withstand harsh conditions. Immobilized Biocatalyst Engineering (IBE) combines protein engineering and enzyme immobilization as a single workflow, allowing the production of immobilized biocatalysts that would not be selected based on their soluble performance.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Sabrina Ait Braham, El Hocine Siar, Sara Arana-Pena, Hossein Bavandi, Diego Carballares, Roberto Morellon-Sterling, Diandra de Andrades, Jakub F. Kornecki, Roberto Fernandez-Lafuente
Summary: This study investigates the impact of glycerol on the stability of 13 immobilized enzymes, finding that its stabilizing effect is enzyme-dependent and influenced by concentration and pH. The results emphasize the importance of considering enzyme type, glycerol concentration, and pH level when using glycerol as a stabilizing agent.
PROCESS BIOCHEMISTRY
(2021)
Article
Green & Sustainable Science & Technology
Ederson Paulo Xavier Guilherme, Leticia Maria Zanphorlin, Amanda Silva Sousa, Renan Yuji Miyamoto, Carlos Giovani Oliveira Bruziquesi, Bruna Mara Aparecida de Carvalho Mesquita, Sergio Henrique Sousa Santos, Elizama Aguiar-Oliveira, Junio Cota
Summary: Integrated bioprocess strategies can facilitate ethanol production from both C6 and C5 fractions of lignocellulosic feedstocks. A new process concept, SSICF, combines hydrolysis of sugarcane bagasse with xylose isomerization and the co-fermentation of C6 and C5 sugars. Using a multi-enzymatic system with recombinant enzymes immobilized in magnetic nanoparticles, SSICF achieved high ethanol global yields with non-GMO yeast, demonstrating industrial potential.
Review
Environmental Sciences
Xing Zhu, Chenxi Du, Bo Gao, Bin He
Summary: High carbon dioxide concentration in the atmosphere calls for eco-friendly mitigation strategies. Carbonic anhydrase (CA) immobilization can enhance its stability and enzyme recycling, but there is limited mass transfer between gaseous CO2 and the active center of immobilized CA. This review focuses on improving the mass transfer process to increase the efficiency of enzymatic CO2 capture for industrial applications.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2023)
Article
Chemistry, Physical
Roha Riaz, Mubeen Ashraf, Nazim Hussain, Zulqarnain Baqar, Muhammad Bilal, Hafiz M. N. Iqbal
Summary: Enzymes have replaced traditional industrial catalysts as more efficient and eco-friendly alternatives. Protein engineering and immobilization techniques have been developed to improve enzyme functionality and stability for industrial applications.
Article
Chemistry, Applied
Javeria Ayub, Muhammad Usama Saeed, Nazim Hussain, Iqra Zulfiqar, Tahir Mehmood, Hafiz M. N. Iqbal, Muhammad Bilal
Summary: Nanobiocatalysis is a technique that combines biotechnology with nanotechnology to enhance bioprocessing capabilities. Immobilized enzymes improve enzyme activity, stability, and reusability. Recent advancements in nano-biotechnology allow for the incorporation of natural biocatalysts into nanostructures that provide additional properties. The use of nanomaterials as carriers for enzymes has led to the development of stable and active nanobiocatalysts.
TOPICS IN CATALYSIS
(2023)
Article
Environmental Sciences
Marise Silva de Carvalho, Luiz Henrique Sales de Menezes, Adriana Bispo Pimentel, Floriatan Santos Costa, Polyane Cabral Oliveira, Marta Maria Oliveira dos Santos, Iasnaia Maria de Carvalho Tavares, Muhammad Irfan, Muhammad Bilal, Joao Carlos Teixeira Dias, Julieta Rangel de Oliveira, Marcelo Franco
Summary: In this study, a comprehensive investigation was conducted on the production of xylanase by solid-state fermentation using Aspergillus oryzae ATCC 10124. Enzyme production was optimized through statistical designs for both the mixture of waste residues and fermentation parameters. The optimized process resulted in a significant increase (up to 165%) in enzymatic activity. The xylanase produced in this fermentation process exhibited alkaline characteristics and high thermal stability. Moreover, the multienzyme extract obtained directly from the fermentation process showed efficient saccharification capabilities without the need for enzyme purification.
WASTE AND BIOMASS VALORIZATION
(2023)
Article
Energy & Fuels
Namra Ahmad, Shakira Aslam, Nazim Hussain, Muhammad Bilal, Hafiz M. N. Iqbal
Summary: Lignin can replace fossil fuels and be degraded into organic compounds that can be converted into biopolymers and other high-value goods. Understanding the mechanisms of lignin degradation by microbial communities, strains, and enzymes can enhance our understanding of aromatic metabolism.
BIOENERGY RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Veer Singh, Jyoti Singh, Nidhi Singh, Sachchida Nand Rai, Manoj Kumar Verma, Manisha Verma, Vishal Singh, Mahesh Sanjay Chivate, Muhammad Bilal, Vishal Mishra
Summary: In this study, the removal of Cd (II), Pb (II), and Cr (VI) at a concentration of 50 mg/L was investigated using Microbacterium paraoxydans strain VSVM IIT(BHU). X-ray photoelectron spectroscopy and energy dispersive X-ray confirmed the binding of heavy metals on the bacterial cell surface, as well as the reduction of Cr (VI) to Cr (III). The dynamics of heavy metal removal were determined to be a combination of diffusion and transfer control based on the dimensionless values of N-k (9.49 x 10(-3), 9.92 x 10(-3), and 1.23 x 10(-2) for Cr (VI), Cd (II), and Pb (II) ions, respectively). The isolated bacterial strain showed a high removal efficiency for heavy metal ions, with maximum values of 91.62% for Cr (VI), 89.29% for Pb (II), and 83.29% for Cd (II) at 50 mg/L.
Review
Biotechnology & Applied Microbiology
Tehreem Mahmood, Nazim Hussain, Areej Shahbaz, Sikandar Mulla, Hafiz M. N. Iqbal, Muhammad Bilal
Summary: The worldwide depletion of fossil fuel reserves and the associated environmental and health risks are discussed in this study. The need for alternative renewable energy sources, with a focus on algae-based biofuels, is emphasized. Different cultivation systems for algae and various factors affecting biomass yield and biofuel production are outlined. The economic benefits of algae-based biofuels are highlighted.
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2023)
Article
Chemistry, Multidisciplinary
Qasim Raza, Ismat Bibi, Farzana Majid, Shagufta Kamal, Sadia Ata, Aamir Ghafoor, Muhammad I. Arshad, Samiah H. Al-Mijalli, Arif Nazir, Munawar Iqbal
Summary: In this study, SrBixAlxFe12-2xO19 nano-hexaferrites were prepared using a micro-emulsion method, and their ferroelectric, dielectric, magnetic, photocatalytic, and antibacterial properties were investigated. The doped materials showed higher polarization and coercivity values. The optical band gap decreased with increasing doping concentration. The highly doped ferrite exhibited excellent photocatalytic activity and antimicrobial action against textile dyes and bacteria. Therefore, it is a crucial candidate for solar light responsive photocatalytic and antimicrobial applications.
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
(2023)
Article
Chemistry, Applied
Sadia Ata, Anila Tabassum, Ifra Shaheed, Ijaz ul Mohsin, Norah Alwadai, Maryam Al Huwayz, Munawar Iqbal, Arif Nazir
Summary: In this study, zinc oxide nanorods (ZnO-NR) were synthesized via the hydrothermal method using ZnCl2 as a precursor. The synthesized ZnO-NR were characterized using advanced techniques and were found to effectively sequester lead ions. The removal of lead ions was achieved under specific conditions, and the lead ion sequestration onto ZnO-NR followed kinetic and isotherm models. The results suggest that ZnO-NR has the potential to be used as an adsorbent for lead ion removal from industrial effluents.
ADSORPTION SCIENCE & TECHNOLOGY
(2023)
Article
Pharmacology & Pharmacy
Dure N. Iqbal, Atira Munir, Mazhar Abbas, Arif Nazir, Zahid Ali, Samar Z. Alshawwa, Munawar Iqbal, Naveed Ahmad
Summary: This study prepared membranes containing aloe vera gel and chitosan, characterized using various techniques. The results showed that the membranes had a porous structure and promising antibacterial activity, making them suitable for biomedical applications.
Article
Chemistry, Multidisciplinary
Anil Kumar Singh, Muhammad Bilal, Teofil Jesionowski, Hafiz M. N. Iqbal
Summary: Endocrine-disrupting chemicals (EDCs) have persistent negative effects on the environment and public health, and there is currently no approved paradigm for integrating EDC-related risks on a global scale. To better understand and predict the consequences of EDCs, a computational framework was used to assess the toxic risks and hazards of seven EDCs. The findings revealed severe toxicological risks posed by EDCs and suggested the potential use of enzymes for green sustainable degradation to mitigate these risks.
SUSTAINABLE CHEMISTRY AND PHARMACY
(2023)
Article
Pharmacology & Pharmacy
Dure Najaf Iqbal, Asia Ashraf, Arif Nazir, Samar Z. Alshawwa, Munawar Iqbal, Naveed Ahmad
Summary: The study successfully fabricated hydrogel membranes composed of oregano essential oil and sodium alginate, and evaluated their antibacterial, antioxidant activity and physicochemical performance. The results showed that membranes with higher content of oregano essential oil exhibited better antibacterial and antioxidant efficacy. Therefore, these membranes have the potential to be used as wound-healing materials.
Article
Chemistry, Physical
Arif Nazir, Shakeel Alam, Norah Alwadai, Mazhar Abbas, Ismat Bibi, Abid Ali, Naveed Ahmad, Maryam Al Huwayz, Munawar Iqbal
Summary: In this study, copper nanoparticles were synthesized using leaves extract of Citrullus colocynthis as a reducing agent. The synthesized nanoparticles exhibited promising photocatalytic and antibacterial activities, as well as significant antioxidant potential. The results highlight the potential of green synthesis for the fabrication of Cu NPs with desirable properties.
ZEITSCHRIFT FUR PHYSIKALISCHE CHEMIE-INTERNATIONAL JOURNAL OF RESEARCH IN PHYSICAL CHEMISTRY & CHEMICAL PHYSICS
(2023)
Article
Engineering, Environmental
Jakub Zdarta, Filip Ciesielczyk, Muhammad Bilal, Katarzyna Jankowska, Karolina Bachosz, Oliwia Degorska, Agnieszka Rybarczyk, Teofil Jesionowski, Anne S. Meyer
Summary: Synthetic hormones are dangerous pollutants that pose risks to the reproductive health of both ecosystems and humans through water, including drinking water. Traditional wastewater treatment methods are not efficient enough. In this study, a new biosystem made of CaSiO3 and laccase was designed and fabricated, and its application for the removal of the synthetic estrogen 17 alpha-ethynylestradiol (EE2) from aqueous systems was demonstrated. The biosystem achieved 100% removal efficiency and showed synergistic degradation through simultaneous adsorption and biocatalytic conversion, with enzymatic conversion dominating.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2023)
Article
Environmental Sciences
Atif Khan Jadoon, Ambreen Sarwar, Maria Faiq Javaid, Amna Shoukat, Munawar Iqbal, Zia ul Haq, Salman Tariq
Summary: This study estimates environmental efficiency using the Malmquist-Luenberger productivity index (MLI) for 43 Asian countries from 1990 to 2019. The results suggest that, on average, environmental efficiency has decreased by 0.3% over this period. Cambodia, Turkey, and Nepal have the highest total factor productivity (TFP) output growth rate on average among the 43 Asian countries, serving as examples of sustainable development that balances environmental protection and efficiency.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Physics, Condensed Matter
M. I. Khan, Saddam Hussain, Muhamad Saleem, Fatimah Mohammed Alzahrani, Muhammad Siddique, M. S. Hassan, Allah Ditta Khalid, Munawar Iqbal
Summary: The sol-gel method was used to deposit Ti-doped MAPbBr3 films on FTO-glass substrates with different doping ratios (0%, 4%, and 6%). XRD analysis confirmed the cubic structure of all films, and the 4% Ti-doped film exhibited a large grain size, low band gap energy, and high refractive index. Solar cells fabricated using the 4% Ti-doped MAPbBr3 film showed improved performance in terms of current density, open circuit voltage, fill factor, and efficiency.
PHYSICA B-CONDENSED MATTER
(2024)
Article
Biochemistry & Molecular Biology
Wei-Han Hui, Yen-Lin Chen, Shu-Wei Chang
Summary: Crosslinking affects the mechanical behavior and tissue metabolism of collagen-based tissues. Aging and diabetes alter the crosslinking type and density, thus changing tissue properties. High-connectivity enzymatic crosslinking and advanced-glycation end-products result in uniform deformation under daily activity, while low-connectivity enzymatic crosslinking does not. High-connectivity model displays more sliding, while AGEs induce instability in structures near the binding sites.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Yao-Bin Ma, Xiao-Qiu Zhou, Wei-Dan Jiang, Pei Wu, Yang Liu, Shu-Wei Li, Ling Tang, Lu Zhang, Hai-Feng Mi, Lin Feng
Summary: Tea polyphenols (TPs) are an inexpensive feed additive that can protect the gill function of fish against Flavobacterium columnare (F. columnare) through various mechanisms, including suppression of oxidative stress, apoptosis, and inflammation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Yan-Jia Guo, Jing-Ru Pang, Yu Zhang, Zhong-Rui Li, Xiao-Lin Zi, Hong-Min Liu, Ning Wang, Li-Juan Zhao, Ya Gao, Bo Wang, Piet Herdewijn, Cheng-Yun Jin, Ying Liu, Yi-Chao Zheng
Summary: This study identifies the conjugation of NEDD8 to LSD1 and its impact on GC cells, revealing the significance of LSD1 neddylation in GC.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Geum-Jae Jeong, Fazlurrahman Khan, Nazia Tabassum, Young-Mog Kim
Summary: Vibrio species are motile gram-negative bacteria commonly found in aquatic environments. They can adapt to environmental fluctuations and develop protective mechanisms against host immune systems. Different types of sigma and anti-sigma factors play important roles in regulating gene expression and virulence in Vibrio species.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Zahra Mohammadbagheri, Behrooz Movahedi, Shaghayegh Saeedi, Abbas Rahmati
Summary: In this study, a reusable biobased composite hydrogel was successfully synthesized and used for the removal of Tl(I) from aqueous solutions. The hydrogel exhibited high adsorption capacity and thermal stability, and the adsorption process was found to be thermodynamically spontaneous. The results demonstrated that the hydrogel can be reused multiple times without significant loss of its adsorption capacity.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Iqra Batool, Muhammad Imran, Ayesha Anwar, Farhan Ahmed Khan, Afrah E. Mohammed, Ashwag Shami, Hafiz Iqbal
Summary: Enzyme immobilization on ZnO/SnO2 and ZnO/SnO2/SA nanocomposites was studied, and the catalytic and biochemical characteristics of the immobilized enzyme were investigated. The results showed that ZnO/SnO2 and ZnO/SnO2/SA nanostructures are suitable carriers for enzyme immobilization.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Elias Mosaffa, Dhruvi Patel, Nasim Amiri Ramsheh, Rishikumar Indravadan Patel, Atanu Banerjee, Hossein Ghafuri
Summary: In this study, a unique 3D bead structure of adsorbent, composed of bacterial cellulose microfilaments reinforced chitosan adorned with melamine 2D plates, was synthesized for anionic dye removal. The adsorbent showed high specific surface area and abundant functional groups, resulting in exceptional adsorption capacity. The characteristics and optimal conditions of the adsorbent were assessed and analyzed.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Carolina Gismene, Jorge Enrique Hernandez Gonzalez, Marilia de Freitas Calmon, Andrey Fabricio Ziem Nascimento, Angela Rocio Nino Santisteban, Felipe Antunes Calil, Alana Della Torre da Silva, Paula Rahal, Rejane Maira Goes, Raghuvir Krishnaswamy Arni, Ricardo Barros Mariutti
Summary: Mammaliicoccus sciuri, a bacterium of clinical and veterinary relevance, expresses the virulence factor Exfoliative Toxin C (ExhC), which binds specifically to desmoglein-1 (Dsg1) and plays a role in tissue integrity. This study investigates the necrotic activity of ExhC and identifies a variant lacking necrotic potential but retaining enzymatic activity through mutagenesis experiments and crystal structure determination.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Bibhas K. Bhunia, Ashutosh Bandyopadhyay, Souradeep Dey, Biman B. Mandal
Summary: Intervertebral disc degeneration is a major cause of chronic low back pain and disability. Researchers have developed a bioactive silk-based hybrid hydrogel system functionalized with decellularized human Wharton's jelly extracellular matrix as bioactive cues. The hydrogels mimic the rheological and mechanical properties of the native nucleus pulposus tissue and support cell viability, proliferation, and tissue maturation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Qun Zhang, Yonggan Yan, Zhao Li, Jing Du, Kai Zhang, Liguo Zhang, Ting Wang, Alberto Bianco, Shaohua Ge, Baojin Ma
Summary: A new crosslinking strategy has been devised to construct injectable sodium alginate hydrogels, which can be regulated for injectability and have great potential in biomedical applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Seong Eun Hong, Ji-Soo Lee, Hyeon Gyu Lee
Summary: The potential of multi-layer nanoemulsions for improving the cellular uptake, antioxidant activity, and in vitro bioaccessibility of alpha-tocopherol was examined. The results showed that the multi-layer nanoemulsions significantly enhanced the cellular uptake and antioxidant activity of alpha-tocopherol compared to free coumarin 6. Furthermore, the use of chitosan and dextran sulfate as wall materials in the multi-layer nanoemulsions led to even higher cellular uptake, in vitro bioaccessibility, and antioxidant activity of alpha-tocopherol.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Jingjing Yu, Yanhui Zhang, Ruoning Zhang, Yanxiang Gao, Like Mao
Summary: High internal phase emulsions (HIPEs) based on beeswax (BW) oleogels and octenyl succinic acid starch (OSA starch) were prepared by a simple one-step method. The results showed that oleogelation improved the stability and encapsulation capability of the HIPEs, which is significant for the development of novel healthy food.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Bi-Feng Yao, Xiu-Ju Luo, Jun Peng
Summary: The balanced and continuous fission and fusion of mitochondria, regulated by OPA1, are important for maintaining mitochondrial dynamics in cardiovascular disorders. Understanding the role of OPA1 in mitochondrial fusion can provide insights into the pathogenesis of cardiovascular diseases.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Gokul Paramasivam, Anandhavelu Sanmugam, Vishnu Vardhan Palem, Murugan Sevanan, Ananda Babu Sairam, Nachiappan Nachiappan, Buhyun Youn, Jung Sub Lee, Muthuchamy Nallal, Kang Hyun Park
Summary: Nanomaterials are being used to deliver therapeutic agents in living systems. Different nanotechnological applications such as immunosensors, immune assays, and drug delivery are being implemented using nanotechnology. Genomics plays a crucial role in selecting appropriate drugs for personalized cancer therapy. It helps in analyzing gene and protein expression, disease classification, and designing disease models. Delivering drugs to specific areas using nanoparticles is a challenging issue.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Jing Li, Wei Wang, Han Wu, Feng Peng, Hui Gao, Ying Guan
Summary: In this study, CMH-based CPF films were prepared and their thermal and barrier properties were successfully regulated. The tensile strength and water vapor barrier property of CPF films were significantly improved, making them promising for food packaging applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)