Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 52, Issue -, Pages 59-65Publisher
ELSEVIER
DOI: 10.1016/j.ijbiomac.2012.09.017
Keywords
Silk; Sericin; Antheraea mylitta; Protein-based material
Funding
- National Research Foundation of Korea (NRF)
- Korean government (MEST) [2011-00073]
- Department of Science and Technology, Indian Government (DST) [13/18 2009]
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Although the technique for extracting the Bombyx mod sericin has been extensively known, the extraction of sericin from wild-silkworm cocoons is not yet standardized. The aim of this study was to find the optimal conditions for the extraction of sericin from Antheraea mylitta cocoons, with high yields and minimum degradation. We attempted to apply various protocols for the extraction of the A. mylitta sericin (AmS). Among these, we found that the extraction of AmS with a sodium carbonate solution exhibited the highest yield except the conventional soap-alkali extraction. To find the optimal conditions for the AmS extraction with the sodium carbonate, we changed the concentration of sodium carbonate and the treatment time. With an increase in the sodium carbonate concentration and the extraction time, the yield of AmS increased, but the molecular weight (MW) of AmS decreased. Considering the yield, molecular weight distribution (MWD) and amino acid composition of AmS, we suggest that the optimal conditions for the AmS extraction require treatment with 0.02 M sodium carbonate and boiling for 60 min. (C) 2012 Elsevier B.V. All rights reserved.
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