Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 45, Issue 4, Pages 421-426Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2009.06.008
Keywords
Liposomes; Copolymer-glucose oxidase conjugate; Glucose-sensitive release
Funding
- BioGreen 21 Program [20080401033]
- Rural Development Administration, Republic of Korea
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Liposomes, which release their contents in response to the concentration of glucose, were prepared by modifying the liposomal surface with the conjugate of poly(N-isopropylacrylamide-co-methacrylic acid-co-octadecylacrylate) (P(NIPAM-co-MAA-co-ODA)) and glucose oxidase (GOD). The maximum enzymatic activity of copolymer conjugated GOD (Polym-GOD) was observed around pH 5.0 and the value was about 40% of that of native GOD. Nine lysine residues per GOD molecule, on average, were found to be covalently attached to the copolymers. Egg phosphatidylcholine liposomes bearing Polym-GOD released their contents in response to the concentration of glucose and the sensitivity was higher than dipalmitoylphosphatidylcholine liposomes. (C) 2009 Elsevier B.V. All rights reserved.
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