Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 44, Issue 1, Pages 86-91Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2008.10.005
Keywords
Acid hydrolysis; Amylopectin; Amylose; Scanning chemical force microscopy (SCFM); Phosphate; Carbon
Funding
- National Center for Genetic Engineering and Biotechnology (BIOTEC)
- The Thailand Research Fund (TRF)
- The Commission on Higher Education
- Postgraduate Education and Research Program in Chemistry (PERCH-CIC)
- The Thai Center of Excellence for Physics (Integrated Physics Cluster)
- Nano-Imaging Unit, Faculty of Science, Mahidol University
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Surface chemical microstructure of hydrochloric acid hydrolyzed tapioca starch producing different amylose:amylopectin (Am:Ap) ratios were studied with scanning chemical force microscopy (CFM). The chemical force probes were functionalized of two types with -OH (phosphate specific) and -CH3 (carbon specific). Lateral force trace-minus-retrace (TMR) images from -OH and -CH3 probes revealed changes in the phosphate domains and the carbon backbone for the varying acid hydrolyzed tapioca starch compared to that of the native tapioca starch. Scanning electron micrographs (SEM) showed different degree of the granule surface disruption before and after hydrolysis. The exterior structures of the acid hydrolyzed starch granules were chemically investigated with CFM to study the relationships of the surface molecular structures and the Am:Ap ratios. (C) 2008 Elsevier B.V. All rights reserved.
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