Review
Medicine, General & Internal
Maxime Foguenne, Serena MacMillan, Philipp Kron, Jay Nath, Arnaud Devresse, Martine De Meyer, Mourad Michel, Sarah Hosgood, Tom Darius
Summary: The use of high-risk renal grafts for transplantation requires the optimization of pretransplant assessment and preservation reconditioning strategies. Active oxygenation plays a central role in dynamic preservation strategies to recondition mitochondria and restore cellular energy. The differences between normothermic and hypothermic machine perfusion, as well as the logistic and economic implications, should be considered for implementation at a local level.
JOURNAL OF CLINICAL MEDICINE
(2023)
Review
Biochemistry & Molecular Biology
Tom Darius, Jay Nath, Michel Mourad
Summary: The use of high-risk renal grafts for transplantation requires optimization of pretransplant preservation and assessment strategies to improve clinical outcomes and decrease organ discard rate. Oxygenation during hypothermic machine preservation is proposed as a resuscitative measure, providing a critical overview of the fundamentals and suggesting different strategies for clinical implementation.
Article
Medicine, General & Internal
Tom Darius, Martial Vergauwen, Louis Maistriaux, Robin Evrard, Andrea Schlegel, Matteo Mueller, Donna O'Neil, Andrew Southam, Selda Aydin, Arnaud Devresse, Martine De Meyer, Pierre Gianello, Christian Ludwig, Philipp Dutkowski, Michel Mourad
Summary: Short bubble and subsequent intermittent surface oxygenation is an effective and cheap preservation strategy during hypothermic machine perfusion (HMP), which can protect mitochondria and eliminate the need/costs of a membrane oxygenator and oxygen source during transport.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Engineering, Biomedical
Daniel J. Portillo, Jose Gonzalez, Carorina Villarreal, Sophia J. Salazar, Anjelyka Fasci, Brandt Wearden, Jessica Oseghale, Anis Khalil, Thomas Perillo, Leslie Muenchow, Robert De Lorenzo, R. Lyle Hood
Summary: This study presents a novel, portable, and nonelectronic device that is capable of delivering oxygenated machine perfusion to different organs. Through an innovative pneumatic circuit system and silicone tube design, the device achieved perfusion parameters consistent with previous kidney preservation studies.
ANNALS OF BIOMEDICAL ENGINEERING
(2022)
Article
Biochemistry & Molecular Biology
Fabio Silvio Taccone, Fuhong Su, Xinrong He, Lorenzo Peluso, Katia Donadello, Sabino Scolletta, Daniel De Backer, Jean-Louis Vincent
Summary: The effects of correcting hypotension with vasopressors in septic shock may improve cerebral oxygenation but do not reverse the alterations in brain microcirculation or cerebral metabolism.
Review
Medicine, General & Internal
Bulent Ergin, Sakir Akin, Can Ince
Summary: AKI is a serious multifactorial condition involving loss of function and damage, with alterations in renal microcirculation and oxygenation being crucial factors. Autoregulation and pathophysiological changes also contribute to the occurrence of AKI.
JOURNAL OF CLINICAL MEDICINE
(2021)
Review
Biochemistry & Molecular Biology
Anna Radajewska, Anna Krzywonos-Zawadzka, Iwona Bil-Lula
Summary: Kidney transplantation is the standard procedure for treating end-stage renal disease, and machine perfusion has been proven to be a better solution for organ preservation and monitoring.
Review
Medicine, General & Internal
Ahmed S. Ghoneima, Richard X. Sousa X. Da Silva, Martina A. Gosteli, Adam D. Barlow, Philipp Kron
Summary: The high demand for organs in kidney transplantation has led to the widespread use of machine perfusion technologies. This study provides a systematic review of the developments in this field over the past 10 years and compares different perfusion techniques. The results show that dynamic preservation strategies, such as hypothermic machine perfusion, have lower rates of delayed graft function and have the potential to improve outcomes in kidney transplantation.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Medicine, General & Internal
Gennady Geskin, Michael D. Mulock, Nicole L. Tomko, Anna Dasta, Sandeep Gopalakrishnan
Summary: This study investigates the effects of lower limb revascularization on the microcirculation and determines if macrovascular and microvascular assessments correlate. The results show that endovascular intervention can decrease arterial Doppler acceleration times and increase oxyhemoglobin, indicating treatment efficacy. However, macrovascular measurements and NIRS metrics of oxygenation and perfusion do not correlate.
Article
Medicine, General & Internal
Eberhard Grambow, Niels Arne Sandkuehler, Justus Gross, Daniel G. E. Thiem, Michael Dau, Matthias Leuchter, Malte Weinrich
Summary: This study investigated the feasibility of using hyperspectral imaging (HSI) to monitor skin oxygenation in patients with peripheral artery disease (PAD). The results showed that HSI can be used as an additional technique to assess tissue oxygen saturation in PAD patients, and it is robust to external confounders such as temperature and physical activity.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Engineering, Electrical & Electronic
Hsiao-Huang Chang, Yung-Chang Chen, Ting-Wei Chiang, Yi-Min Wang, Chia-Wei Sun
Summary: In this study, a novel method was proposed to monitor lower limb microcirculation using functional near-infrared spectroscopy (NIRS) in patients with extracorporeal membrane oxygenation (ECMO). By controlling the speed of the ECMO system and measuring hemodynamics with NIRS devices, features were extracted from blood oxygen data and used as inputs for machine learning classification. Patients were divided into two groups based on discharge and mortality. In venovenous (VV) ECMO, a classification model based on the characteristics of this type showed better discriminating ability in effectively distinguishing the two groups.
IEEE JOURNAL OF SELECTED TOPICS IN QUANTUM ELECTRONICS
(2023)
Review
Urology & Nephrology
B. Mesnard, A. E. Ogbemudia, G. Karam, F. Dengu, G. Hackim, J. Rigaud, G. Blancho, S. Drouin, M. O. Timsit, J. Branchereau
Summary: The main objective of static cold storage is to reduce cellular metabolic demands in order to extend the period of ischaemia prior to transplantation. Halt metabolic demands to extend ischaemia time. Introduction of oxygenation during organ preservation may help ameliorate ischaemia reperfusion injury and improve outcomes.
WORLD JOURNAL OF UROLOGY
(2022)
Review
Medicine, General & Internal
Sara Akalay, Sarah A. Hosgood
Summary: Although machine perfusion is gradually being applied in clinical practice, the improvement of organ reconditioning therapies is still at the experimental stage, showing a translational gap. This review discusses the biological processes and current strategies and interventions for preventing ischemia-reperfusion injury, treating its deleterious consequences, or supporting the reparative response of the kidney, and explores prospects to improve the clinical translation of these therapies by addressing multiple aspects of ischemia-reperfusion injury to achieve robust and long-lasting protective effects on the kidney graft.
JOURNAL OF CLINICAL MEDICINE
(2023)
Review
Urology & Nephrology
Asel S. Arykbaeva, Dorottya K. de Vries, Jason B. Doppenberg, Marten A. Engelse, Thomas Hankemeier, Amy C. Harms, Leonie G. Wijermars, Alexander F. Schaapherder, Jaap A. Bakker, Rutger J. Ploeg, Ian P. J. Alwayn, Jan H. N. Lindeman
Summary: Normothermic machine perfusion (NMP) is a novel preservation strategy that maintains organs in a metabolically active state, providing superior organ preservation and allowing viability testing before transplantation. However, current perfusion protocols may not fully address the metabolic requirements of marginal donor grafts. Monitoring of metabolic status during NMP is important for optimal outcomes.
KIDNEY INTERNATIONAL
(2021)
Article
Dermatology
Yitian Fang, Lisanne van Ooijen, Gisela Ambagtsheer, Anton V. V. Nikolaev, Marian C. C. Clahsen-van Groningen, Jenny Dankelman, Ron W. F. de Bruin, Robert C. C. Minnee
Summary: This study aimed to assess the value of laser speckle contrast imaging (LSCI) in visualizing renal cortical perfusion and investigate the additional value of dual-side LSCI measurements compared to single aspect measurement during normothermic machine perfusion (NMP). The results showed that LSCI fluxes correlated linearly with renal blood flow during NMP. LSCI can visualize and semiquantify renal cortical perfusion and dual-side LSCI measurements provide added value compared to single aspect measurement in evaluating renal function.
LASERS IN SURGERY AND MEDICINE
(2023)
Review
Cell Biology
Sarah Schaefer, Alma Zernecke
Summary: CD8(+) T cells play an important role in atherosclerosis by regulating inflammation, cell death, and plaque formation, showing therapeutic potential in treating atherosclerosis.
Review
Cardiac & Cardiovascular Systems
Edoardo Bertero, Jan Dudek, Clement Cochain, Murilo Delgobo, Gustavo Ramos, Brenda Gerull, Takahiro Higuchi, Martin Vaeth, Alma Zernecke, Stefan Frantz, Ulrich Hofmann, Christoph Maack
Summary: The interplay between the cardiovascular system, metabolism, and inflammation plays a central role in the pathophysiology of cardiovascular diseases. This paper provides an overview of the interrelation between inflammation and metabolism, discusses the involvement of disrupted immuno-metabolic interface in cardiovascular disease, and presents new technologies and therapeutic approaches.
CARDIOVASCULAR RESEARCH
(2022)
Review
Biochemistry & Molecular Biology
Kristina Andelovic, Patrick Winter, Peter Michael Jakob, Wolfgang Rudolf Bauer, Volker Herold, Alma Zernecke
Summary: Atherosclerosis is an inflammatory disease of large and medium-sized arteries characterized by the growth of atherosclerotic lesions. Low and oscillatory shear stress may contribute to arterial remodeling and increased pulse wave velocity. Cardiovascular magnetic resonance imaging enables evaluation of dynamic processes and assessment of hemodynamic parameters for early identification of high-risk plaques.
Review
Cardiac & Cardiovascular Systems
Jenifer Vallejo, Clement Cochain, Alma Zernecke, Klaus Ley
Summary: Single-cell RNA sequencing has been instrumental in studying immune cells in atherosclerosis, identifying new immune cell subsets and significantly upregulated genes, providing a new perspective on the pathogenesis of atherosclerosis.
CARDIOVASCULAR RESEARCH
(2021)
Article
Cardiac & Cardiovascular Systems
Jesus Gil-Pulido, Nuria Amezaga, Ivana Jorgacevic, Helga D. Manthey, Melanie Roesch, Theresa Brand, Peter Cidlinsky, Sarah Schafer, Andreas Beilhack, Antoine-Emmanuel Saliba, Kristina Lorenz, Louis Boon, Immo Prinz, Ari Waisman, Thomas Korn, Clement Cochain, Alma Zernecke
Summary: IL-23R(+) gamma delta T cells predominantly found in the aortic root promote early atherosclerotic lesion formation, plaque necrosis, and inflammation at this site. Targeting IL-23R may be explored as a therapeutic approach to mitigate atherosclerotic lesion development.
CARDIOVASCULAR RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Annabelle Rosa, Elke Butt, Christopher P. Hopper, Stefan Loroch, Markus Bender, Harald Schulze, Albert Sickmann, Sandra Vorlova, Peter Seizer, David Heinzmann, Alma Zernecke
Summary: Cyclophilin A (CyPA) is a widely expressed protein in both prokaryotic and eukaryotic cells. Recent studies have suggested the possibility of acetylation at certain sites in CyPA. In this study, the researchers investigated whether acetylation at these sites plays a role in the release of CyPA from platelets. The results confirmed the presence of CyPA in platelets and its release upon stimulation, but no acetylation was observed. These findings suggest that acetylation is not a major protein modification in platelets and is not required for the release of CyPA from platelets.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Kristina Andelovic, Patrick Winter, Thomas Kampf, Anton Xu, Peter Michael Jakob, Volker Herold, Wolfgang Rudolf Bauer, Alma Zernecke
Summary: Using high-resolution MRI, this study examined the temporal and spatial changes in flow, wall shear stress (WSS), oscillatory shear index (OSI), and elastic properties in healthy and atherosclerotic mice during ageing and atherosclerosis. The results revealed distinct differences in local hemodynamic profiles between healthy and atherosclerotic mice, with circumferential WSS identified as a potential marker of plaque size and composition, and radial strain as a potential marker for vascular elasticity. Monitoring local aortic hemodynamics through 2D projection maps of WSS and OSI could significantly enhance our understanding of the impact of hemodynamics on atherosclerosis and plaque progression.
Article
Biochemistry & Molecular Biology
Heike Braun, Michael Hauke, Robert Eckenstaler, Markus Petermann, Anne Ripperger, Niklas Kuehn, Edzard Schwedhelm, Beatrice Ludwig-Kraus, Frank Bernhard Kraus, Virginie Dubourg, Alma Zernecke, Barbara Schreier, Michael Gekle, Ralf A. Benndorf
Summary: The study found that vascular smooth muscle cell (VSMC)-specific TP deletion attenuated atherogenesis in mice, without impacting blood pressure or lipid profiles. Additionally, 8-iso-PGF2 alpha appears to reduce atherogenesis in Ldlr-deficient mice, especially in those lacking EC-specific TP. This suggests that the TP expressed in VSMC is involved in atherosclerotic lesion formation and 8-iso-PGF2 alpha has an inhibitory effect on atherogenesis in this experimental model.
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Article
Cardiac & Cardiovascular Systems
Giuseppe Rizzo, Julius Gropper, Marie Piollet, Ehsan Vafadarnejad, Anna Rizakou, Sourish Reddy Bandi, Panagiota Arampatzi, Tobias Krammer, Nina DiFabion, Oliver Dietrich, Anahi Paula Arias-Loza, Marco Prinz, Matthias Mack, Kai Schlepckow, Christian Haass, Jean Sebastien Silvestre, Alma Zernecke, Antoine Emmanuel Saliba, Clement Cochain
Summary: The study utilized single-cell immune profiling to map the transition of monocyte/macrophage populations in the infarcted heart, revealing the origin and dynamic changes of cardiac macrophages. In summary, the research provides valuable insights into how these cells can be potentially modulated to enhance post-ischaemic heart repair.
CARDIOVASCULAR RESEARCH
(2023)
Article
Cardiac & Cardiovascular Systems
Alma Zernecke, Florian Erhard, Tobias Weinberger, Christian Schulz, Klaus Ley, Antoine Emmanuel Saliba, Clement Cochain
Summary: This study aimed to refine the nomenclature of mononuclear phagocytes in atherosclerotic vessels and compare their transcriptomic profiles in mouse and human disease. The researchers integrated single-cell RNA-sequencing datasets from both mice and humans, identifying subpopulations and unique transcriptomic features. They found conserved transcriptomic features of macrophages and DCs in atherosclerosis, highlighting the relevance of mouse models in studying mononuclear phagocytes in this disease.
CARDIOVASCULAR RESEARCH
(2023)
Article
Multidisciplinary Sciences
Rida Al-Rifai, Marie Vandestienne, Jean-Remi Lavillegrand, Tristan Mirault, Julie Cornebise, Johanne Poisson, Ludivine Laurans, Bruno Esposito, Chloe James, Olivier Mansier, Pierre Hirsch, Fabrizia Favale, Rayan Braik, Camille Knosp, Jose Vilar, Giuseppe Rizzo, Alma Zernecke, Antoine-Emmanuel Saliba, Alain Tedgui, Maxime Lacroix, Lionel Arrive, Ziad Mallat, Soraya Taleb, Marc Diedisheim, Clement Cochain, Pierre-Emmanuel Rautou, Hafid Ait-Oufella
Summary: JAK2V617F mutation drives vascular resident macrophages toward a pathogenic phenotype and promotes dissecting aortic aneurysm.
NATURE COMMUNICATIONS
(2022)
Article
Medicine, Research & Experimental
Haroon Shaikh, Joern Pezoldt, Zeinab Mokhtari, Juan Gamboa Vargas, Duc-Dung Le, Josefina Pena Mosca, Estibaliz Arellano Viera, Michael Ag Kern, Caroline Graf, Niklas Beyersdorf, Manfred B. Lutz, Angela Riedel, Maike Buettner-Herold, Alma Zernecke, Hermann Einsele, Antoine-Emmanuel Saliba, Burkhard Ludewig, Jochen Huehn, Andreas Beilhack
Summary: This study reveals the immunoprotective role of FRC niches in secondary lymphoid organs for regulating aGvHD and highlights the framework of cellular and molecular interactions that modulate CD4+ T cell alloimmunity.
Correction
Multidisciplinary Sciences
Rida Al-Rifai, Marie Vandestienne, Jean-Remi Lavillegrand, Tristan Mirault, Julie Cornebise, Johanne Poisson, Ludivine Laurans, Bruno Esposito, Chloe James, Olivier Mansier, Pierre Hirsch, Fabrizia Favale, Rayan Braik, Camille Knosp, Jose Vilar, Giuseppe Rizzo, Alma Zernecke, Antoine-Emmanuel Saliba, Alain Tedgui, Maxime Lacroix, Lionel Arrive, Ziad Mallat, Soraya Taleb, Marc Diedisheim, Clement Cochain, Pierre-Emmanuel Rautou, Hafid Ait-Oufella
NATURE COMMUNICATIONS
(2022)
Article
Gastroenterology & Hepatology
Knud Esser, Xiaoming Cheng, Jochen M. Wettengel, Julie Lucifora, Lea Hansen-Palmus, Katharina Austen, Armando A. Roca Suarez, Sarah Heintz, Barbara Testoni, Firat Nebioglu, Minh Tu Pham, Shangqing Yang, Alma Zernecke, Dirk Wohlleber, Marc Ringelhan, Mathias Broxtermann, Daniel Hartmann, Norbert Hueser, Julia Mergner, Andreas Pichlmair, Wolfgang E. Thasler, Mathias Heikenwalder, Georg Gasteiger, Andreas Blutke, Axel Walch, Percy A. Knolle, Ralf Bartenschlager, Ulrike Protzer
Summary: Hepatitis B virus (HBV) can enter hepatocytes by binding with lipoproteins and utilize the cholesterol transport pathway of macrophages to deliver itself to hepatocytes. This transmission through the lipid transport pathway to the liver may involve transinfection of liver macrophages and result in deposition of HBV in the perisinusoidal space.
CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY
(2023)
Article
Cardiac & Cardiovascular Systems
Patrick Winter, Kristina Andelovic, Thomas Kampf, Jan Hansmann, Peter Michael Jakob, Wolfgang Rudolf Bauer, Alma Zernecke, Volker Herold
Summary: Wall shear stress (WSS) and pulse wave velocity (PWV) are important parameters for blood flow in the vessel wall, but are challenging to measure simultaneously with flow-sensitive phase-contrast cardiovascular magnetic resonance (CMR). A flexible reconstruction framework was used in this study to quantify both PWV and WSS in the murine aortic arch from the same 4D flow measurement in only 35 min, improving the analyses and information content of in vivo measurements.
JOURNAL OF CARDIOVASCULAR MAGNETIC RESONANCE
(2021)