Journal
INTERNATIONAL JOURNAL FOR PARASITOLOGY
Volume 42, Issue 5, Pages 429-435Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.ijpara.2012.02.013
Keywords
Leishmania donovani; Recombinant p45; Methionine aminopeptidase; Immunogenicity; T-cell response; Cured Leishmania hamsters; Cured Leishmania patients and endemic contacts; Prophylactic potential
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Funding
- University Grants Commission (UGC), India
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The development of a vaccine against visceral leishmaniasis (VL) conferring long-lasting immunity remains a challenge. Identification and proteomic characterization of parasite proteins led to the detection of p45, a member of the methionine aminopeptidase family. To our knowledge the present study is the first known report that describes the molecular and immunological characterization of p45. Recombinant Leishmania donovani p45 (rLdp45) induced cellular responses in cured hamsters and generated Th1-type cytokines from peripheral blood mononuclear cells of cured/endemic VL patients. Immunization with rLdp45 exerted considerable prophylactic efficacy (similar to 85%) supported by an increase in mRNA expression of iNOS, IFN-gamma, TNF-alpha and IL-12 and decrease in TGF-beta and IL-4, indicating its potential as a vaccine candidate against VL. (C) 2012 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.
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