Article
Biochemistry & Molecular Biology
Chunqun Wang, Wenjie Gao, Shi Yan, Xing-Quan Zhu, Xun Suo, Xin Liu, Nishith Gupta, Min Hu
Summary: N-glycosylation is a physiologically vital post-translational modification in eukaryotic organisms, and Haemonchus contortus has exclusive chitobiose modifications in its N-glycans. Immunogenic proteins like peptidases are N-glycosylated in the parasite, with glycan-rich conjugates primarily located in the intestine and gonads of adult worms, evading host immune responses. This comprehensive atlas of N-glycosylation in the prevalent parasitic nematode highlights its importance for infection, immunity, and prevention.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2021)
Article
Chemistry, Multidisciplinary
Xiaocui Zheng, Yiran Li, Tongxiao Cui, Jing Yang, Xiangfeng Meng, Haiqi Wang, Liusheng Chen, Jian He, Nan Chen, Liying Meng, Lin Ding, Ran Xie
Summary: Executing precise glycan editing is crucial for understanding glycan-related biological processes and improving disease therapy. This study introduces a versatile and traceless glycan modification method that allows customization of targeted protein glycoforms through chemical and photoregulation. The method minimizes interference and enables precise temporal control of editing events.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Chemistry, Multidisciplinary
Mingli Liu, Xianjin Qin, Xin-Shan Ye
Summary: Glycans have become a hot topic due to their biological activities, but their heterogeneity and complexity hinder research progress. Therefore, developing glycan assembly strategies to obtain structurally well-defined glycans is an important issue in carbohydrate chemistry.
Article
Cell Biology
Belinda L. Spillings, Christopher J. Day, Albert Garcia-Minambres, Anupriya Aggarwal, Nicholas D. Condon, Thomas Haselhorst, Damian F. J. Purcell, Stuart G. Turville, Jennifer L. Stow, Michael P. Jennings, Johnson Mak
Summary: This study reveals that HIV is captured near the lymphocyte surface in a virion-glycan-dependent manner. Removing certain glycans impairs virus-cell binding and interfering with glycan interactions reduces HIV infectivity. These findings provide new insights into the interaction between HIV and host cells.
Article
Chemistry, Analytical
Mathieu Lamarre, Thomas Tremblay, Marc-Antoine Bansept, Karine Robitaille, Vincent Fradet, Denis Giguere, Denis Boudreau
Summary: In this study, a sensor immobilized with Tn antigen on a surface coated with polydopamine/polyethylene oxide showed high reliability and sensitivity in prostate cancer diagnostics. Detection limits of anti-Tn antibodies were validated in different concentration ranges and successfully used to discriminate blood serum samples from prostate cancer patients and cancer-free individuals.
Article
Chemistry, Organic
Zhiyuan Chen, Guozhi Xiao
Summary: The highly branched tetradecasaccharide repeating unit and shorter sequences of GLSWA-1 from Ganoderma lucidum, which possess immune-enhancing activities, were prepared using a one-pot glycan assembly strategy that features orthogonal one-pot glycosylation, one-pot assembly of oligosaccharides, and modular and convergent [4+5+5] assembly of the highly branched tetradecasaccharide.
Article
Biology
Lucia Sanjurjo, Iris A. Schulkens, Pauline Touarin, Roy Heusschen, Ed Aanhane, Kitty C. M. Castricum, Tanja D. De Gruijl, Ulf J. Nilsson, Hakon Leffler, Arjan W. Griffioen, Latifa Elantak, Rory R. Koenen, Victor L. J. L. Thijssen
Summary: The study reveals that specific chemokine-galectin interactions can modulate the immunoregulatory function of galectins, affecting their glycan-binding activity and specificity. In particular, binding of CXCL4 changes the carbohydrate binding site of galectin-1, increasing its apoptotic activity on CD8(+) T cells.
COMMUNICATIONS BIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Julia Koenig-Beihammer, Ulrike Vavra, Yun-Ji Shin, Christiane Veit, Clemens Gruenwald-Gruber, Yasmin Gillitschka, Jasmin Huber, Manuela Hofner, Klemens Vierlinger, Dieter Mitteregger, Andreas Weinhaeusel, Richard Strasser
Summary: Glycosylation plays an important role in the infectivity and immune evasion of viral envelope proteins. The heavily glycosylated spike protein of SARS-CoV-2 is influenced by host-derived glycan modifications, which impact immunogenic epitopes, virus-cell interaction, and virus transmission. In this study, recombinant variants of the SARS-CoV-2 receptor-binding domain (RBD) were produced with blood group antigens using an in vivo glycoengineering approach in Nicotiana benthamiana plants. The glycosylation variants with blood group antigens were shown to be functional and could potentially provide protection against virus infection in non-infected RBD-negative blood group O individuals.
FRONTIERS IN CHEMISTRY
(2022)
Review
Biochemistry & Molecular Biology
Yoichiro Harada, Yuki Ohkawa, Kento Maeda, Naoyuki Taniguchi
Summary: The endoplasmic reticulum (ER) contains multiple quality control systems (QCS) that shape the glycoproteome of eukaryotic cells. One of these systems, glycan QCS, plays a crucial role in ensuring proper glycoprotein folding while also potentially increasing the risk of overall hypoglycosylation, leading to protein misfolding and cellular stress response activation.
Review
Chemistry, Multidisciplinary
Yujun Kim, Ji Young Hyun, Injae Shin
Summary: This review discusses the applications of glycan microarrays in studying glycan-protein interactions. It describes the methods for preparing glycan probes and constructing glycan microarrays, and highlights their use in profiling glycan-binding patterns and analyzing substrate specificities of enzymes. It also explores other important uses of glycan microarrays, such as identifying ligands for lectins and functional glycans within cells.
CHEMICAL SOCIETY REVIEWS
(2022)
Article
Plant Sciences
Jennifer Schwestka, Julia Koenig-Beihammer, Yun-Ji Shin, Ulrike Vavra, Nikolaus F. Kienzl, Clemens Gruenwald-Gruber, Daniel Maresch, Miriam Klausberger, Elisabeth Laurent, Maria Stadler, Gabriele Manhart, Jasmin Huber, Manuela Hofner, Klemens Vierlinger, Andreas Weinhaeusel, Ines Swoboda, Christoph J. Binder, Wilhelm Gerner, Florian Grebien, Friedrich Altmann, Lukas Mach, Eva Stoeger, Richard Strasser
Summary: The receptor binding domain (RBD) of the SARS-CoV-2 spike protein is crucial in virus-host cell interaction and infection, with plant-produced RBD potentially leading to false positive reactions in serological assays.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Ji Young Hyun, Sanggil Kim, Chang-Hee Lee, Hyun Soo Lee, Injae Shin
Summary: This study presents a general method for efficient preparation of homogeneous glycoproteins using genetic code expansion and chemoselective ligation techniques. The glycoproteins produced through this method can recognize mammalian cell-surface lectins and enter cells, showing potential for biomedical applications.
ACS CHEMICAL BIOLOGY
(2021)
Article
Biology
Tetsuya Hirata, Misaki Takata, Yuko Tokoro, Miyako Nakano, Yasuhiko Kizuka
Summary: The activity of GnT-V in cells is regulated by changing the maturity of cellular N-glycans. Altering the glycosylation state affects the secretion and protein level of GnT-V, which in turn impacts its promotion of cancer growth and malignancy.
COMMUNICATIONS BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
J. Sebastian Temme, Jennifer A. Crainic, Laura M. Walker, Weizhun Yang, Zibin Tan, Xuefei Huang, Jeffrey C. Gildersleeve
Summary: This study has identified 26 anti-glycan antibodies, most of which bind microbial carbohydrates, and found that these antibodies predominantly originate from IgG+ memory B cells, rather than from naive, early emigrant, or immature B cells.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Biology
Tessa J. Nash, Katrina M. Morris, Neil A. Mabbott, Lonneke Vervelde
Summary: Researchers have developed complex 3D chicken enteroids that mimic intestinal organization, allowing for investigation of intestinal cell biology and host-pathogen interactions. These enteroids can develop optimally in suspension and have successfully been infected with Salmonella Typhimurium, influenza A virus, and Eimeria tenella without the need for micro-injection, providing a comprehensive model for exploring epithelial and leukocyte interactions in host-pathogen, food science, and pharmaceutical research.
COMMUNICATIONS BIOLOGY
(2021)
Letter
Infectious Diseases
Lei Zha, Shirong Li, Zhichu Ren, Xiang Li, Dayan Zhang, Yi Zou, Lingling Pan, Qiancheng Xu, Zuqin Rui, Shanghua Chen, Gang Yang, Zhixiang Chen, Boris Tefsen, Jun Guo
JOURNAL OF INFECTION
(2022)
Article
Environmental Sciences
Xiaokai Zhang, Boling Li, Daniel N. Schillereff, Richard C. Chiverrell, Boris Tefsen, Mona Wells
Summary: The study investigates the impact of soil/sediment on bioreporter signals, highlighting the importance of considering this when assessing pollutant bioavailability. Neglecting this effect can lead to significant errors in results. Recommendations for different experimental settings were provided to ensure accurate measurements.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Engineering, Environmental
Boling Li, Xiaokai Zhang, Boris Tefsen, Mona Wells
Summary: Due to the large number of contaminated sites, bioavailability-based measurement and modeling of toxicity are used for screening response, although they are relatively cumbersome. This study demonstrates the use of high throughput lights-on bioreporter technology to measure both speciation and toxicity of cadmium and lead. The bioreporter has a strong predictive ability for toxicity endpoints, making it useful for assessing the risk of aquatic ecotoxicity and setting environmental water quality standards.
Review
Veterinary Sciences
Tessa Nash, Lonneke Vervelde
Summary: There is a growing interest in studying the avian intestine and its role in dietary components, probiotic microorganisms, and infectious diseases. However, the lack of physiologically relevant models has hindered research in this field. This review discusses various in vitro tools that aim to mimic the intestinal environment, from simple cell lines to complex 3D enteroids, highlighting their benefits, limitations, and potential applications in intestinal biology and pathology research. The review also emphasizes the need for improved models that incorporate microbiota and a dynamic mechanical environment.
Editorial Material
Engineering, Environmental
Xiaokai Zhang, Damia Barcelo, Robert J. Clougherty, Bin Gao, Hauke Harms, Boris Tefsen, Meththika Vithanage, Hailong Wang, Zhenyu Wang, Mona Wells
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2022)
Article
Infectious Diseases
Lei Zha, Xiang Li, Zhichu Ren, Dayan Zhang, Yi Zou, Lingling Pan, Shirong Li, Shanghua Chen, Boris Tefsen
Summary: Based on a retrospective cohort study, piperacillin/tazobactam could be an effective alternative to carbapenems for treating nosocomial pneumonia caused by ESBL-producing K. pneumoniae when the minimum inhibitory concentration (MIC) is <= 8 mg/L.
Article
Biochemistry & Molecular Biology
Trillion Surya Lioe, Ziwen Xie, Jianfang Wu, Wenlong Li, Li Sun, Qiaoli Feng, Raju Sekar, Boris Tefsen, David Ruiz-Carrillo
Summary: In this study, we identified and characterized a dipeptidyl peptidase from Mycobacterium tuberculosis (MtDPP) that specifically cleaves proline residues at the P-1 substrate position and exhibits high thermal stability. We also found that alanine replacements of residues in the active site of MtDPP resulted in loss of catalysis. Furthermore, we demonstrated that MtDPP activity is inhibited by known DPP4 inhibitors and can truncate the C-X-C motif chemokine ligand 10, suggesting a potential role in immune modulation for this mycobacterial enzyme.
BIOLOGICAL CHEMISTRY
(2023)
Article
Engineering, Chemical
Paul Craig, Ruben Ng, Boris Tefsen, Sam Linsen, Yu Liu, Joshua Hendel
Summary: Biochips are engineered substrates that change colour according to biochemical reactions, and can detect multiple analytes simultaneously. Chip designs that use a combination of spots are more efficient but challenging to design. This study explores the potential of information visualization and machine learning techniques to improve biochip design.
Article
Infectious Diseases
Lei Zha, Xue Zhang, Yusheng Cheng, Qiancheng Xu, Lingxi Liu, Simin Chen, Zhiwei Lu, Jun Guo, Boris Tefsen
Summary: The benefit of combining polymyxin and tigecycline for treating carbapenem-resistant bacterial infections is uncertain. A cohort study found that adding polymyxin B to high-dose tigecycline therapy did not lead to better clinical outcomes in pneumonia patients caused by carbapenem-resistant Klebsiella pneumoniae and Acinetobacter baumannii. The study recommended against adding polymyxin B to the high-dose tigecycline regimen for this type of pneumonia.
Article
Agriculture, Dairy & Animal Science
M. A. Hussein, F. Khattak, L. Vervelde, S. Athanasiadou, J. G. M. Houdijk
Summary: The effects of amino acid supplementation on broilers placed on recycled litter were investigated. The results showed that placement on recycled litter improved growth performance, while amino acid supplementation did not significantly increase growth performance.
Article
Veterinary Sciences
Tessa J. Nash, Katrina M. Morris, Neil A. Mabbott, Lonneke Vervelde
Summary: Researchers have found that chicken enteroids can replicate the complexity and functions of the avian intestine in vitro, showing high reproducibility and similarity to the in vivo intestine. This finding provides a physiologically-relevant model for studying the avian gut.
VETERINARY RESEARCH
(2023)
Article
Fisheries
Safieh Zeinali, Kate Sutton, Lonneke Vervelde
Summary: Chickens have a unique immune structure characterized by the absence of draining lymph nodes and the presence of well-developed mucosa-associated lymphoid tissue. The structure and development of chicken lymphoid tissues in the intestine, particularly Peyer's patches, have been poorly studied. However, the use of CSF1R-eGFP reporter transgenic chickens has allowed for the visualization and investigation of the development and distribution of these tissues. This research provides insights that could benefit mucosal vaccination strategies and the development of targeted vaccine delivery systems.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Article
Virology
Samantha Sives, Sarah Keep, Erica Bickerton, Lonneke Vervelde
Summary: Avian coronavirus (IBV) is an economically important infectious disease that affects chickens worldwide. This study identified shared and serotype-specific immunogenic epitopes of the S glycoprotein of three major circulating strains of IBV, which may contribute to the development of a broader cross-protective IBV vaccine.
Article
Veterinary Sciences
Brigid Orr, Kate Sutton, Sonja Christian, Tessa Nash, Helle Niemann, Lone Lind Hansen, Mike J. McGrew, Stina Rikke Jensen, Lonneke Vervelde
Summary: The 2D enteroids model developed in chicken recapitulates major differentiated cell lineages and shows tight cell layer formation with minimal paracellular flux, robust epithelial integrity, and appropriate innate immune responses. This model serves as a valuable tool for studying intestinal cell biology and innate immune function, with potential applications in screening of various compounds.
VETERINARY RESEARCH
(2021)
