Article
Microbiology
Martina Koehsler, Edwin Kniha, Angelika Wagner, Julia Walochnik
Summary: In this study, a diagnostic PCR protocol was established to determine the prevalence of E. gingivalis in Austria. The results showed that nearly 50% of the 59 participants were positive for E. gingivalis, with a significantly higher prevalence in participants with self-reported gingivitis. A potentially new subtype, ST3, was identified in addition to the established subtypes ST1 and ST2. Interestingly, ST2 and ST1/ST3 were more often associated with gingivitis.
Review
Immunology
Shanshan Wang, France Moreau, Kris Chadee
Summary: Gasdermins (GSDMs) are a group of proteins that can induce cell death or pyroptosis by forming pores in the plasma membrane. This review provides a comprehensive overview of the expression, activation, biological functions, and regulation of GSDMs, with a focus on their role in host defense against Eh and other protozoan parasitic infections.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Microbiology
Gilbert O. Silveira, Helena S. Coelho, Adriana S. A. Pereira, Patricia A. Miyasato, Daisy W. Santos, Lucas F. Maciel, Giovanna G. G. Olberg, Ana C. Tahira, Eliana Nakano, Maria Leonor S. Oliveira, Murilo S. Amaral, Sergio Verjovski-Almeida
Summary: This study reveals the importance of long non-coding RNAs (lncRNAs) in the homeostasis and fertility of adult schistosomes. Differential expression of lncRNAs between paired and unpaired worms suggests their role in cell proliferation, pairing, female reproductive capacity, and/or egg development. In vitro and in vivo silencing experiments demonstrate the potential of lncRNAs as therapeutic target candidates.
Article
Biochemistry & Molecular Biology
Juan Deng, Ya-Jing Liu, Wen-Tian Wei, Qi-Xuan Huang, Li-Ping Zhao, Ling-Yun Luo, Qi Zhu, Lin Zhang, Yuan Chen, Yan-Ling Ren, Shan-Gang Jia, Yu-Luan Lin, Ji Yang, Feng-Hua Lv, Hong-Ping Zhang, Feng-E Li, Li Li, Meng-Hua Li
Summary: We characterized the developmental changes of the rumen in sheep and goats by profiling single-cell transcriptomes. Through comprehensive transcriptome and metagenome atlases, we identified key transitional signatures associated with the development of ruminal cells, microbiota, and core transcriptional regulatory networks. We also revealed potential cross-talk between host cells and microbiomes and their roles in modulating gene expression in ruminal cells.
Article
Biochemistry & Molecular Biology
Guenole Le Pennec, Cas Retel, Vienna Kowallik, Lutz Becks, Philine G. D. Feulner
Summary: Through experimental research on a host-parasite system, we found that population structure, selection, and their interaction play a crucial role in genetic diversity. Populations that experience both selection and population structure fluctuations have higher genetic diversity, providing empirical support for improving theoretical models of adaptation during host-parasite co-evolution.
Article
Parasitology
Deiviane Aparecida Calegar, Kerla Joeline Lima Monteiro, Polyanna Araujo Alves Bacelar, Brenda Bulsara Costa Evangelista, Mayron Morais Almeida, Jessica Pereira dos Santos, Marcio Neves Boia, Beatriz Coronato-Nunes, Lauren Hubert Jaeger, Filipe Anibal Carvalho-Costa
Summary: This study investigated the diversity, phylogenetic relationships, prevalence, and distribution of tetra- and octonucleated cyst-producing Entamoeba in different Brazilian regions. Different species were identified, with E. coli and E. histolytica showing unique prevalence patterns in different biomes. The molecular epidemiology of Entamoeba spp. presents important implications for public health and warrants further exploration.
PARASITES & VECTORS
(2021)
Review
Cell Biology
Lotem Sarid, Serge Ankri
Summary: This article discusses the role of host microbiota in regulating epigenetic processes in Entamoeba histolytica, as well as the implications of this knowledge for the development of new anti-amebic strategies.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Plant Sciences
Okhee Choi, Seunghoe Kim, Byeongsam Kang, Yeyeong Lee, Juyoung Bae, Jinwoo Kim
Summary: This study analyzed the genetic diversity of Korean B. glumae isolates, revealing that the isolates were divided into 11 clusters and three divisions, with most of the pigment-producing isolates grouped in divisions II and III.
Article
Genetics & Heredity
Marc Teissier, Luiz F. Brito, Flavio S. Schenkel, Guido Bruni, Pancrazio Fresi, Beat Bapst, Christele Robert-Granie, Helene Larroque
Summary: This study aimed to evaluate the genetic connectedness and population structure of Alpine and Saanen dairy goats from four countries and assess the feasibility of international genomic evaluation. The results showed that French and Italian dairy goat populations are more genetically related, while Canadian dairy goats have significant genetic differences with European populations. These findings are important for further investigating the accuracy of genomic breeding values in dairy goat populations.
FRONTIERS IN GENETICS
(2022)
Article
Microbiology
Hanbang Zhang, Juliana Veira, Sarah Twitty Bauer, Christopher Yip, Upinder Singh
Summary: This study characterizes, for the first time, the components of the RNA-induced silencing complex (RISC) in Entamoeba, revealing multiple functional protein components and novel nuclear assembly protein-interacting partners. The research further enhances our understanding of the molecular regulatory mechanisms of RNAi in this parasite.
Article
Multidisciplinary Sciences
Layla Hockerstedt, Elina Numminen, Ben Ashby, Mike Boots, Anna Norberg, Anna-Liisa Laine
Summary: This study found that isolated host populations are more affected by pathogen infection, while connected host populations have higher levels of resistance diversity. Spatial structure and host gene flow play important roles in the impacts of pathogens on hosts.
NATURE COMMUNICATIONS
(2022)
Article
Microbiology
Maria Razzauti, Guillaume Castel, Jean-Francois Cosson
Summary: The study revealed that the genetic diversity of bank voles and PUUV is positively correlated with forest coverage and contiguity of habitats. While the genetic diversity of bank voles was weakly structured in space, that of PUUV showed strong structuring, which has important implications for understanding the distribution and epidemiology of PUUV.
Article
Multidisciplinary Sciences
Rachel Sendor, Kristin Banek, Melchior M. Kashamuka, Nono Mvuama, Joseph A. Bala, Marthe Nkalani, Georges Kihuma, Joseph Atibu, Kyaw L. Thwai, W. Matthew Svec, Varun Goel, Tommy Nseka, Jessica T. Lin, Jeffrey A. Bailey, Michael Emch, Margaret Carrel, Jonathan J. Juliano, Antoinette Tshefu, Jonathan B. Parr
Summary: This study estimated the incidence and prevalence of non-falciparum and Plasmodium falciparum malaria infections in Kinshasa, Democratic Republic of Congo. The findings suggest that while P. falciparum remains the primary driver of malaria, non-falciparum species also pose an infection risk.
NATURE COMMUNICATIONS
(2023)
Article
Agriculture, Dairy & Animal Science
Eric R. Morgan, Anne Segonds-Pichon, Hubert Ferte, Patrick Duncan, Jacques Cabaret
Summary: Parasite overdispersion among hosts has important implications for their population dynamics and control. The factors determining parasite overdispersion, such as parasite acquisition and acquired host immunity, have not been thoroughly studied in parasite populations.
Article
Plant Sciences
Jing Zhang, Dajian Pan, Zhilan Fan, Hang Yu, Liqun Jiang, Shuwei Lv, Bingrui Sun, Wenfeng Chen, Xingxue Mao, Qing Liu, Chen Li
Summary: The study developed 1,592 SNPs for genetic analysis of 998 O. rufipogon accessions from Guangdong and Hainan Provinces in China. The results showed that O. rufipogon accessions had rich genetic diversity, with variations observed among different regions. A core collection was constructed based on genetic distance, capturing over 99% of the genetic variation.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Infectious Diseases
Roya Salehi, Ali Rostami, Hamed Mirjalali, Christen Rune Stensvold, Ali Haghighi
Summary: The study in Iran found multiple Blastocystis subtypes in farm animals, with ST1 and ST3 present in both human and non-human hosts, and ST2, ST5, ST6, and ST10 found exclusively in specific groups.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Immunology
Daniela Michlmayr, Luis Alves de Sousa, Luise Muller, Pikka Jokelainen, Steen Ethelberg, Lasse Skafte Vestergaard, Susanne Schjorring, Sarah Mikkelsen, Carl Widstrup Jensen, Lasse Dam Rasmussen, Christen Rune Stensvold
Summary: Enterocytozoon bieneusi was identified as the cause of a foodborne outbreak of diarrhea affecting >70 individuals. The outbreak was associated with consuming food from a workplace canteen, with symptoms including diarrhea, fatigue, and abdominal pain. The study highlights the importance of investigating E. bieneusi in foodborne outbreak investigations.
CLINICAL INFECTIOUS DISEASES
(2022)
Article
Microbiology
Damien Mornico, Chung-Chau Hon, Mikael Koutero, Christian Weber, Jean-Yves Coppee, C. Graham Clark, Marie-Agnes Dillies, Nancy Guillen
Summary: The study profiles the transcriptomes of closely related species to Entamoeba histolytica and reveals the similarities and differences in their gene regulatory sequences. The initiation of antisense transcription is found to be a common feature among all Entamoeba species, particularly in genes involved in processes common to species infecting the human intestine.
Article
Microbiology
Christen Rune Stensvold, Brede Aksdal Sorland, Rebecca P. K. D. Berg, Lee O'Brien Andersen, Mark van der Giezen, Joanna L. Bowtell, Ayman A. El-Badry, Salem Belkessa, Ozgur Kurt, Henrik Vedel Nielsen
Summary: Using metabarcoding and bioinformatics analyses, differences were identified in stool microbiota diversity between Blastocystis-positive and Blastocystis-negative individuals. Blastocystis carriers showed higher alpha diversity and enrichment of Firmicutes and Bacteroidetes at the phylum level, while non-carriers showed enrichment of Proteobacteria. The presence of certain genera also differed between carriers and non-carriers. Blastocystis-positive stools appeared to have gut microbiomes associated with eubiosis, while Blastocystis-negative stools showed similarities to dysbiosis-related microbiomes.
Article
Microbiology
Christen Rune Stensvold, Kasandra Ascuna-Durand, Amal Chihi, Salem Belkessa, Ozgur Kurt, Ayman El-Badry, Mark van der Giezen, C. Graham Clark
Summary: In this study, the genetic diversity of Entamoeba coli and Entamoeba hartmanni was further investigated, revealing the existence of previously identified subtypes as well as a potentially new subtype. The subtypes showed varying degrees of genetic homogeneity and intrasubtype diversity. Additionally, substantial variation was found in E. hartmanni, but no links were found between subtype and geographical origin.
JOURNAL OF EUKARYOTIC MICROBIOLOGY
(2023)
Article
Microbiology
Isbene Sanchez, Alejandro Dashti, Pamela C. Koster, Begona Bailo, Nuria Gonzalez, Janire Allende, Christen Rune Stensvold, David Carmena, David Gonzalez-Barrio
Summary: The enteric protozoan parasites Cryptosporidium spp., Giardia duodenalis, and Dientamoeba fragilis contribute to gastrointestinal illness in high-income countries, and a new multiplex real-time PCR assay has been developed to detect these pathogens with high sensitivity and specificity.
Article
Hematology
Christina Schjellerup Eickhardt-Dalboge, Anna Caecilia Ingham, Lee O'Brien Andersen, Henrik V. Nielsen, Kurt Fuursted, Christen Rune Stensvold, Morten Kranker Larsen, Lasse Kjaer, Sarah Friis Christensen, Trine Alma Knudsen, Vibe Skov, Christina Ellervik, Lars Ronn Olsen, Hans Carl Hasselbalch, Xiaohui Chen Nielsen, Jens Jorgen Elmer Christensen
Summary: Chronic inflammation is important in the development of PV and gut microbiota is believed to be associated with inflammation. The study found that PV patients have an abnormal gut microbiota compared to healthy controls.
Article
Medicine, General & Internal
Andreas Arnholdt Pedersen, Gitte Nyvang Hartmeyer, Christen Rune Stensvold, Raquel Martin-Iguacel
Summary: This case report presents a patient with S. stercoralis hyperinfection syndrome involving the central nervous system, highlighting the global significance of Strongyloides CNS infection in both endemic and non-endemic regions.
Article
Biochemistry & Molecular Biology
Milan Jirku, Andrea Kasparova, Zuzana Lhotska, Miroslav Obornik, Kristyna Brozova, Klara J. Petrzelkova, Peter Samas, Oldriska Kadlecova, Christen Rune Stensvold, Katerina Jirku
Summary: This study focuses on the epidemiology of Dientamoeba fragilis in humans and animals, and the analysis of samples reveals the prevalence rates and related factors of this protist in healthy individuals.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Kristina Zahonova, Ross S. Low, Christopher J. Warren, Diego Cantoni, Emily K. Herman, Lyto Yiangou, Claudia A. Ribeiro, Yasinee Phanprasert, Ian R. Brown, Sonja Rueckert, Nicola L. Baker, Jan Tachezy, Emma L. Betts, Eleni Gentekaki, Mark van der Giezen, C. Graham Clark, Andrew P. Jackson, Joel B. Dacks, Anastasios D. Tsaousis
Summary: Blastocystis is a prevalent microbial eukaryote in the gut of humans and animals, but its role as a commensal or parasite is still debated. By studying Proteromonas lacertae, a close relative of Blastocystis, through genomic analysis, the evolution of Blastocystis from a canonical protist to a gut microbe has been examined in detail.
Article
Gastroenterology & Hepatology
Munkhtsetseg Banzragch, Kemal Sanli, Christen Rune Stensvold, Ozgur Kurt, Sule Ari
Summary: The microbiota composition of colonic cleansing fluid (CCF) collected during colonoscopy has been found to distinguish patients with inflammatory bowel disease (IBD) from healthy controls, suggesting that CCF analysis can be an alternative strategy for early diagnosis and disease progression in IBD biomarker research.
SCANDINAVIAN JOURNAL OF GASTROENTEROLOGY
(2023)
Article
Environmental Sciences
Musamba Banza, Hilary Rutto, Tumisang Seodigeng
Summary: This study examined the kinetics of Copper (II) and Lead (II) leaching in EDTA acid. The impacts of various factors were investigated to improve leaching conditions and assess leaching kinetics. The results showed that increasing temperature, stirring speed, and acid concentration, as well as decreasing solid-liquid ratio and pH, enhanced the leaching of copper (II) and lead (II).
SOIL & SEDIMENT CONTAMINATION
(2023)
Editorial Material
Public, Environmental & Occupational Health
Tine Graakjaer Larsen, Jonas Kahler, Marianne Lebbad, Huma Aftab, Luise Mueller, Steen Ethelberg, Lihua Xiao, Christen Rune Stensvold
TRAVEL MEDICINE AND INFECTIOUS DISEASE
(2023)
Review
Microbiology
Christina Schjellerup Eickhardt-Dalboge, Anna Cacilia Ingham, Henrik V. Nielsen, Kurt Fuursted, Christen Rune Stensvold, Lee O'Brien Andersen, Morten Kranker Larsen, Lasse Kjaer, Sarah Friis Christensen, Trine Alma Knudsen, Vibe Skov, Christina Ellervik, Lars Ronn Olsen, Hans Carl Hasselbalch, Jens Jorgen Elmer Christensen, Xiaohui Chen Nielsen
Summary: This study investigates the gut microbiota in patients with essential thrombocythemia (ET) and found that they have higher richness and different bacterial composition compared to healthy controls. The relative abundance of certain bacteria within the Firmicutes phylum is lower in ET patients, including an important immunoregulative bacterium Faecalibacterium. These findings suggest a potential role of gut microbiota in immune dysregulation of ET patients.
MICROBIOLOGY SPECTRUM
(2023)
