Article
Microbiology
Shweta Murthy, Akash Suresh, Debabrata Dandasena, Sakshi Singh, Madhusmita Subudhi, Vasundhra Bhandari, Vandna Bhanot, Jaspreet Singh Arora, Paresh Sharma
Summary: Accurate quantification of pathogens is crucial for preventing their spread. Droplet digital PCR (ddPCR) is a highly effective method for absolute parasite quantification. In this study, ddPCR was shown to be as effective as qPCR in detecting and quantifying Theileria parasites, but more sensitive at lower parasite DNA concentrations. The ddPCR method was highly accurate and reproducible, making it a valuable resource for various applications.
Article
Parasitology
Hong-xi Zhao, Xia Li, Jun-long Liu, Gui-quan Guan, Xin-gang Dan
Summary: This study analyzed the metabolites and metabolic pathways in Theileria annulata-infected cells after treatment with buparvaquone (BW720c) and found that BW720c treatment induces metabolic disturbances and host cell apoptosis.
PARASITES & VECTORS
(2022)
Article
Parasitology
Zhi Li, Junlong Liu, Shuaiyang Zhao, Quanying Ma, Zhihong Guo, Aihong Liu, Youquan Li, Guiquan Guan, Jianxun Luo, Hong Yin
Summary: This study reveals for the first time that Theileria annulata-secreted SVSP455 molecule directly interacts with both exogenous and endogenous bovine HSP60 protein, and that the interaction of SVSP455-HSP60 may manipulate the host cell apoptosis signaling pathway. These results provide insights into cancer-like phenotypes underlying Theileria transformation and therapeutics for protection against other pathogens.
PARASITES & VECTORS
(2022)
Article
Immunology
Zhi Li, Junlong Liu, Shuaiyang Zhao, Quanying Ma, Aihong Liu, Youquan Li, Guiquan Guan, Jianxun Luo, Hong Yin
Summary: The study focused on the SVSP family member TA05575 of T. annulata for its expression profiles and subcellular distribution in different life cycle stages. Results showed TA05575 had significantly higher transcription levels in the schizont stage and mainly localized in the nucleus of infected cells. Interaction between TA05575 and RBMX2-like protein was confirmed, providing new insights into the mechanisms of cell transformation by T. annulata.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Veterinary Sciences
Hong-xi Zhao, Xia Li, Jun-long Liu, Gui-quan Guan, Jian-xun Luo
Summary: In this study, the bovine TFG gene was identified and found to be closely associated with the expression of TFG protein in transformed cells of Theileria annulata. The findings suggest an interaction between the TFG protein and T. annulata.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Parasitology
Prasanna Babu Araveti, Macha Vijay, Prajna Parimita Kar, Shalu Varunan, Anand Srivastava
Summary: This study screened a drug library and identified two compounds, MMV000062 and MMV560185, with potential inhibitory activity against T. annulata infected bovine leucocytes. These compounds were non-toxic to host cells and MMV560185 was found to kill infected cells through the TNFR-1 mediated extrinsic pathway of apoptosis. These findings suggest that MMV560185 may be a candidate for chemotherapeutics of tropical theileriosis.
INTERNATIONAL JOURNAL FOR PARASITOLOGY-DRUGS AND DRUG RESISTANCE
(2022)
Article
Microbiology
Junlong Liu, Shuaiyang Zhao, Zhi Li, Zhigang Zhang, Baocai Zhao, Guiquan Guan, Hong Yin, Jianxun Luo
Summary: Telomere length and telomerase activity are increased after T. annulata infection in bovine cells, which is dependent on the presence of parasites. Inhibition of parasites or their related pathways can decrease telomerase activity and the expression level of bTERT.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Khawla Elati, Shahin Tajeri, Isaiah Obara, Moez Mhadhbi, Erich Zweygarth, Mohamed Aziz Darghouth, Ard Menzo Nijhof
Summary: This study conducted a transcriptome analysis of attenuated and virulent Theileria annulata, revealing differentially expressed genes and metabolic pathways associated with attenuation. The findings contribute to a better understanding of the attenuation process and the identification of biomarkers.
SCIENTIFIC REPORTS
(2023)
Article
Microbiology
Tomas Javier Poklepovich, Maria Mesplet, Romina Gallenti, Monica Florin-Christensen, Leonhard Schnittger
Summary: Babesia bovis and Theileria annulata are tick-borne hemoprotozoans that differ in various aspects such as invertebrate host specificity and erythrocyte invasion mechanism. Differences in the degradome of proteinases between these two pathogens may be related to their distinct life history features.
Article
Biochemistry & Molecular Biology
Shahin Tajeri, Malak Haidar, Takaya Sakura, Gordon Langsley
Summary: Theileria are tick-transmitted parasites that cause fatal leuko-proliferative diseases in cattle, but their transformation of leukocytes is reversible with anti-theilerial drug treatment. Theileria-transformed leukocytes disseminate in infected cattle causing cancer-like disease. Cytokines, noncoding RNAs, and oncometabolites may contribute to the transformed phenotype and disease pathology.
MOLECULAR MICROBIOLOGY
(2021)
Article
Parasitology
Zhi Li, Junlong Liu, Quanying Ma, Aihong Liu, Youquan Li, Guiquan Guan, Jianxun Luo, Hong Yin
Summary: In this study, the transcription of SVSP454 from Theileria annulata was found in different life stages, with the highest expression during the schizont stage. Interacting proteins of SVSP454, CCDC181 and MRPL30, were screened using a Y2H system. Co-IP and BiFC assays confirmed that SVSP454 directly interacts with CCDC181 and MRPL30 of the host, suggesting a potential regulatory axis involved in the microtubule network and translation process of the host.
PARASITES & VECTORS
(2021)
Article
Environmental Sciences
Yaamini R. Venkataraman, Amanda Shore, Sukanya Dayal, James Sanghyun Lee, Mahsa Alidoost Salimi, Grace Crandall, Malina M. Loeher, Mark Stoops, Megan Swanger, Morgan E. Eisenlord, Kathryn L. Van Alstyne, Mark D. Fast, Colleen A. Burge, Maya L. Groner
Summary: This study explores the effects of temperature and a wetland pathogen on seagrass, finding that the infection caused by the pathogen has a more pronounced impact on the physiological response of seagrass than temperature. By combining whole organism physiological assays with transcriptomic analysis, this study reveals the host-pathogen interaction between seagrass and the pathogen.
FRONTIERS IN MARINE SCIENCE
(2023)
Article
Parasitology
Rupesh Verma, Giridhari Das, Suman Kumar, Subhradal Nath, Ajay Rai, Ashish Soni, Sanju Mandal
Summary: The present study aimed to investigate an outbreak of Theileria annulata infection in a dairy cattle farm in Madhya Pradesh, India, using clinical and molecular techniques. The study found a high prevalence of T. annulata infection in the farm, with different PCR techniques showing positive results in a significant proportion of the samples. Genetic analysis revealed the evolutionary history and population dynamics of T. annulata in India. The study emphasizes the importance of prompt diagnosis and management of T. annulata outbreaks for improving disease control strategies.
PARASITOLOGY RESEARCH
(2023)
Article
Microbiology
Prasanta Kumar Koustasa Mishra, Anupama Jena, Souti Prasad Sarkhel, Sujit Kumar Behera, Annada Das, Thankappan Sabarinath, Dayanidhi Jena, Kruti Debnath Mandal, Adhikari Sahu, Anshuman Kumar, Vinod Kumar, Rahul Ganpatrao Kadam, Srinivas Sathapathy, Thavitiki Prasada Rao
Summary: Tick-borne diseases, such as Theileriosis, can cause significant losses in the dairy sector. This study explored the diagnostic potential of an immunogenic peptide, TaSP-34, for detecting Theileria annulata. The results showed that the IgM-based peptide ELISA had high sensitivity and specificity compared to PCR and protein-based ELISA. This suggests that TaSP-34 peptide can be an efficient diagnostic candidate for T. annulata.
Review
Biochemistry & Molecular Biology
Asahiro Morishita, Kyoko Oura, Tomoko Tadokoro, Koji Fujita, Joji Tani, Tsutomu Masaki
Summary: The liver is a non-immunological organ involved in metabolic activities, nutrient storage, and detoxification. Resident immune cells in the liver drive immunological reactions and certain microRNAs are associated with the immune response to pathogens, although the exact mechanism of miRNA interference in these interactions remains unclear.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Sunil W. Kolte, Stephen D. Larcombe, Suresh G. Jadhao, Swapnil P. Magar, Ganesh Warthi, Nitin V. Kurkure, Elizabeth J. Glass, Brian R. Shiels
Article
Agriculture, Dairy & Animal Science
S. Tsairidou, A. R. Allen, R. Pong-Wong, S. H. McBride, D. M. Wright, O. Matika, C. M. Pooley, S. W. J. McDowell, E. J. Glass, R. A. Skuce, S. C. Bishop, J. A. Woolliams
Article
Multidisciplinary Sciences
Amin Tahoun, Kirsty Jensen, Yolanda Corripio-Miyar, Sean McAteer, David G. E. Smith, Tom N. McNeilly, David L. Gally, Elizabeth J. Glass
SCIENTIFIC REPORTS
(2017)
Article
Multidisciplinary Sciences
Kirsty Jensen, Joanne M. Stevens, Elizabeth J. Glass
Article
Biochemical Research Methods
Heather A. Mathie, Kirsty Jensen, Joanne M. Stevens, Elizabeth J. Glass, Jayne C. Hope
JOURNAL OF MICROBIOLOGICAL METHODS
(2020)
Article
Agriculture, Dairy & Animal Science
K. Bakshy, D. Heimeier, J. C. Schwartz, E. J. Glass, S. Wilkinson, R. A. Skuce, A. R. Allen, J. Young, J. C. McClure, J. B. Cole, D. J. Null, J. A. Hammond, T. P. L. Smith, D. M. Bickhart
Summary: The study developed genetic markers in cattle using new assembly methods and gene references, although no significant associations with bovine tuberculosis phenotypes were found, some markers were successful in predicting the effects on phenotypes.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Multidisciplinary Sciences
Stephen D. Larcombe, Paul Capewell, Kirsty Jensen, William Weir, Jane Kinnaird, Elizabeth J. Glass, Brian R. Shiels
Summary: Background knowledge of factors influencing infection outcome is crucial. Cattle infected by Theileria annulata show a wide range of disease severity, with Holstein cattle being susceptible and Sahiwal cattle being tolerant. Divergent pathogen-host molecular interactions that influence chromatin architecture of the infected cell are a major determinant in gene expression differences linked to disease susceptibility.
Article
Multidisciplinary Sciences
Hayet Benyedem, Abdelmalek Lekired, Moez Mhadhbi, Mokhtar Dhibi, Rihab Romdhane, Soufiene Chaari, Mourad Rekik, Hadda-Imene Ouzari, Tarek Hajji, Mohamed Aziz Darghouth
Summary: Ticks are important vectors for pathogens affecting humans and animals, and they also carry diverse microbiota. A study in Tunisia found that different species of ticks have different microbial diversity, some of which are zoonotic bacteria. This research helps to understand the transmission mechanism of pathogens through tick vectors.
Article
Veterinary Sciences
Kethusegile Raphaka, Enrique Sanchez-Molano, Smaragda Tsairidou, Osvaldo Anacleto, Elizabeth Janet Glass, John Arthur Wooffiams, Andrea Doeschl-Wilson, Georgios Banos
FRONTIERS IN VETERINARY SCIENCE
(2018)
Article
Immunology
Kirsty Jensen, Iain J. Gallagher, Nicholas Johnston, Michael Welsh, Robin Skuce, John L. Williams, Elizabeth J. Glass
INFECTION AND IMMUNITY
(2018)
Article
Biotechnology & Applied Microbiology
S. Wilkinson, S. C. Bishop, A. R. Allen, S. H. McBride, R. A. Skuce, M. Bermingham, J. A. Woolliams, E. J. Glass
Article
Veterinary Sciences
Sean R. Wattegedera, Yolanda Corripio-Miyar, Yvonne Pang, David Frew, Tom N. McNeilly, Javier Palarea-Albaladejo, Colin J. McInnes, Jayne C. Hope, Elizabeth J. Glass, Gary Entrican
VETERINARY RESEARCH
(2017)
Article
Genetics & Heredity
Kethusegile Raphaka, Oswald Matika, Enrique Sanchez-Molano, Raphael Mrode, Mike Peter Coffey, Valentina Riggio, Elizabeth Janet Glass, John Arthur Woolliams, Stephen Christopher Bishop, Georgios Banos
Article
Veterinary Sciences
Kirsty Jensen, Iain J. Gallagher, Anna Kaliszewska, Chen Zhang, Oluyinka Abejide, Maurice P. Gallagher, Dirk Werling, Elizabeth J. Glass
VETERINARY RESEARCH
(2016)