Article
Multidisciplinary Sciences
Daniel Aridgides, John Dessaint, Graham Atkins, James Carroll, Alix Ashare
Summary: Data on adverse events in research bronchoscopy with BAL in CF patients is lacking. We investigated safety of bronchoscopy in adult CF patients, finding that CF subjects had similar adverse event profiles to healthy controls, but with higher rates of fevers, particularly in those with lower FEV1 values. Our data suggest that research bronchoscopy with BAL is safe in CF subjects, especially if limited to those with an FEV1 > 65% predicted.
Article
Infectious Diseases
Nilufer Aylin Acet-Ozturk, Dilara Omer-Topcu, Kubra Vurat Acar, Ozge Aydin-Guclu, Ibrahim Ethem Pinar, Ezgi Demirdogen, Asli Gorek-Dilektasli, Esra Kazak, Vildan Ozkocaman, Ahmet Ursavas, Fahir Ozkalemkas, Beyza Ener, Ridvan Ali, Halis Akalin
Summary: This study aims to evaluate the impact of posaconazole prophylaxis and empiric antifungal treatment on the diagnostic accuracy of galactomannan (GM) measurements in bronchoalveolar lavage (BAL), bronchial lavage (BL), and serum among patients with hematological malignancies.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2023)
Article
Medicine, General & Internal
Vanessa Vedder, Verena Schildgen, Jessica Lusebrink, Ramona Liza Tillmann, Birgitta Domscheit, Wolfram Windisch, Christian Karagiannidis, Michael Brockmann, Oliver Schildgen
Summary: The study compared the cytological profiles of BALs from patients infected with various respiratory pathogens, finding that different pathogens caused distinct cell pattern changes but no general cell distribution pattern. Although the cytology pattern of BAL fluid is not specific enough to be used as the sole diagnostic criterion for COVID-19, it may support clinical decision-making.
Article
Respiratory System
Lingfang Liang, Minfei Hu, Yuanling Chen, Lingke Liu, Lei Wu, Chengcheng Hang, Xiaofei Luo, Xuefeng Xu
Summary: This study found distinct respiratory metabolome characterization in children with PW compared to controls, with glycerophospholipid metabolism pathway being the most relevant. Gender, prematurity, and systemic corticoids use impacted airway metabolite compositions. For PW children with recurrence, premature birth was associated with increased abundance of butyrylcarnitine. These findings suggest an early alteration in lung metabolites could be associated with wheezing development and recurrence later in life.
RESPIRATORY RESEARCH
(2022)
Article
Infectious Diseases
Alexandra Kavvalou, Florian Stehling, Sarah Christina Goretzki, Christian Dohna-Schwake, Peter-Michael Rath, Mathis Steindor
Summary: In this study, the prevalence of Mucorales in pediatric airways was found to be low, and pediatric BAL samples were not frequently contaminated by Mucorales.
INFECTIOUS DISEASES
(2023)
Review
Cardiac & Cardiovascular Systems
Sumita Agrawal, Akhil Dhanesh Goel, Nitesh Gupta, Hari Kishan Gonuguntla, Henri Colt
Summary: This study reviewed the diagnostic efficacy of bronchoalveolar lavage (BAL) in detecting SARS-CoV-2 in cases where upper respiratory tract samples were non-diagnostic. The results showed that BAL can be used to diagnose primary disease and superadded infections in patients with severe COVID-19 lower respiratory tract infection.
Article
Biochemistry & Molecular Biology
Arunava Ghosh, Raymond D. Coakley, Neil E. Alexis, Robert Tarran
Summary: This study explores the impact of increased protease levels in vapers' airways on ENaC activity and airway dehydration. The findings demonstrate that elevated protease levels in vapers' airways have functional significance since they can activate ENaC, resulting in airway dehydration. This is important because lung dehydration contributes to diseases like cystic fibrosis, chronic obstructive pulmonary disease, and asthma.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Environmental Sciences
Swamita Arora, Sangeetha Gupta, Wasim Akram, Ahmed E. Altyar, Priti Tagde
Summary: This study aimed to investigate the effect of a TLR3/dsRNA complex inhibitor on Poly(I:C)-induced airway inflammation following TLR3 activation and to explore a potential pathway for the treatment of asthma through TLR3 inhibition.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Microbiology
John B. O'Connor, Brandie D. Wagner, J. Kirk Harris, Daniel N. Frank, Diana E. Clabots, Theresa A. Laguna
Summary: This study characterized the lower airway mycobiome in children with and without cystic fibrosis using a novel SSU-rRNA gene sequencing approach. The results showed that CF patients had higher fungal load and neutrophilic inflammation in the lower airway, with increased abundance of Aspergillus and Candida. However, there were no clear differences in clustering between CF and disease control samples.
FRONTIERS IN MICROBIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Zuzanna Stachowiak, Beata Narozna, Aleksandra Szczepankiewicz
Summary: Due to their conservation and role in signaling pathways, non-coding RNAs (ncRNAs) are promising biomarkers in respiratory diseases. MicroRNAs (miRNAs), long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) have been extensively studied in these diseases. However, there is limited information on lncRNAs and circRNAs, and only a few studies have been conducted on the least invasive biosample material, exhaled breath condensate (EBC). Further research is needed to understand the complexity of their role and interactions in respiratory diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, General & Internal
Jun Wei, Jun Sun, Xuefen Shuai, Junqing Ren, Xuedong Chen
Summary: The BALF melting curve method is more sensitive in diagnosing PTB compared to other conventional methods, and when combined with other tests, the positive predictive values are significantly increased.
PAKISTAN JOURNAL OF MEDICAL SCIENCES
(2022)
Article
Veterinary Sciences
Hannah Gareis, Lina Hoerner-Schmid, Yury Zablotski, Jelena Palic, Silke Hecht, Bianka Schulz
Summary: This study aimed to evaluate whether treatment improves radiographic abnormalities in cats with FLAD and if these changes correlate with clinical signs. The results showed significant improvement in both clinical and radiographic scores during the treatment period, but there was no correlation between the two. Therefore, repeated radiographic examination can be used as a diagnostic tool to evaluate treatment response in FLAD cats.
JOURNAL OF VETERINARY INTERNAL MEDICINE
(2023)
Review
Respiratory System
Brent A. Bagley, Prabhu Senthil-Kumar, Lauren E. Pavlik, Fatima M. Nabi, Molly E. Lee, Brett C. Hartman, Babar A. Khan, Joseph P. Smith, W. Graham Carlos
Summary: Care of critically injured burn patients poses unique challenges to intensivists. This review provides a comprehensive overview of clinical care for these complex patients, including airway management, postburn complications, volume resuscitation, nutrition, and end-of-life care.
ANNALS OF THE AMERICAN THORACIC SOCIETY
(2022)
Article
Immunology
Xi Chen, Fang Liu, Baoying Zheng, Xiaohui Kang, Xiaolin Wang, Wenjun Mou, Hui Zhang, Anxia Jiao, Shunying Zhao, Jingang Gui
Summary: This study reveals the molecular changes in severe MPP children by analyzing T cells in the BALF, including the expression of cytokines/chemokines, cell activation, exhaustion, and apoptosis. It also provides clues about the possible cell source of proinflammatory cytokines/chemokines from the BALF mixture.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Veterinary Sciences
Jenni Monki, Markku Saastamoinen, Ninja Karikoski, Minna Rajamaki, Marja Raekallio, Jouni Junnila, Susanna Sarkijarvi, Marianna Norring, Anna Valros, Silja Oranen Ben Fatma, Anna Mykkanen
Summary: This study aimed to investigate the effects of bedding materials on lower airway inflammation in horses, finding that peat caused less neutrophilic lower airway inflammation compared to wood shavings. The information gained from this study may help veterinarians and horse owners in selecting bedding materials, particularly for horses suffering from equine asthma.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Pharmacology & Pharmacy
Jacqueline A. Gibbons, Michiel de Vries, Walter Krauwinkel, Yoshiaki Ohtsu, Jan Noukens, Jan-Stefan van der Walt, Roelof Mol, Joyce Mordenti, Taoufik Ouatas
CLINICAL PHARMACOKINETICS
(2015)
Article
Pharmacology & Pharmacy
Jacqueline A. Gibbons, Taoufik Ouatas, Walter Krauwinkel, Yoshiaki Ohtsu, Jan-Stefan van der Walt, Vanessa Beddo, Michiel de Vries, Joyce Mordenti
CLINICAL PHARMACOKINETICS
(2015)
Article
Biochemical Research Methods
Yoshiaki Ohtsu, Shohei Otsuka, Takeshi Nakamura, Kiyoshi Noguchi
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2015)
Article
Pharmacology & Pharmacy
Yoshiaki Ohtsu, Jacqueline A. Gibbons, Katsuhiro Suzuki, Michael E. Fitzsimmons, Kohei Nozawa, Hiroshi Arai
EUROPEAN JOURNAL OF DRUG METABOLISM AND PHARMACOKINETICS
(2017)
Article
Pharmacology & Pharmacy
Yoshiaki Ohtsu, Yoko Susaki, Kiyoshi Noguchi
EUROPEAN JOURNAL OF DRUG METABOLISM AND PHARMACOKINETICS
(2018)
Editorial Material
Biochemical Research Methods
Yoshiro Saito, Noriko Katori, Yoshiaki Ohtsu
Editorial Material
Biochemical Research Methods
Yoshiaki Ohtsu, Takehisa Matsumaru, Masataka Katashima, Masaaki Kakehi, Hiroyuki Kakuo, Takayoshi Suzuki, Masanari Mabuchi, Ryosuke Nakamura, Takahiro Nakamura, Noriko Katori, Seiji Tanaka, Yoshiro Saito
Article
Pharmacology & Pharmacy
Kevin Duisters, Shinji Ogino, Tomohiro Andou, Kazumi Ito, Takafumi Akabane, Amy Harms, Matthijs Moerland, Yuka Hashimoto, Ayumi Ando, Yoshiaki Ohtsu, Naoya Wada, Hideo Yukinaga, Jacqueline Meulman, Hiroyuki Kobayashi, Nobuhiro Kobayashi, Kenichi Suzumura, Thomas Hankemeier
CLINICAL PHARMACOLOGY & THERAPEUTICS
(2020)
Editorial Material
Biochemical Research Methods
Yoshihisa Sano, Yoshiaki Ohtsu, Harue Igarashi, Jun Hosogi, Junji Komaba, Masanari Mabuchi, Takeru Yamaguchi, Hiroshi Kamimori, Tomoko Arakawa, Takahiro Nakamura, Keiko Nakai, Hisanori Hara, Masaaki Kakehi, Sakiko Suzuki, Hitoshi Uchiyama, Yosuke Kawai, Akemi Nagao, Yumi Nishiguchi
Article
Biochemical Research Methods
Yoshiaki Ohtsu, Seiji Tanaka, Harue Igarashi, Masaaki Kakehi, Tamiki Mori, Takahiro Nakamura, Rui Ohashi, Hiroyuki Shimizu, Yutaka Yasuda, Takashige Okayama, Hiroyuki Kakuo, Hiroyuki Yokoi, Mizuki Horiuchi, Masataka Katashima, Ryosuke Nakamura, Kosuke Saito, Yoshiro Saito
Summary: Biomarkers are crucial for drug development, but assessment of quantitative analytical methods for biomarkers is not included in regulatory documents in Japan. A research group in Japan, comprised of industry and regulatory experts, discussed and prepared a 'points to consider document' for validation of biomarker analytical methods, aiming to contribute to global harmonization in this field.
Article
Biochemical Research Methods
Hitoshi Uchiyama, Takeru Yamaguchi, Koji Arai, Tomoko Arakawa, Harue Igarashi, Yoshiaki Ohtsu, Ryosuke Ochiai, Yosuke Kawai, Nozomu Koseki, Junji Komaba, Yoshihisa Sano, Hisao Shimizu, Makoto Takahashi, Akemi Nagao, Takahiro Nakamura, Yumi Nishiguchi, Katsutomo Hata, Keiko Nakai, Hisanori Hara, Masanari Mabuchi, Naoaki Murao, Ryosuke Yoshinaga
Summary: The 12th Japan Bioanalysis Forum Symposium, with the theme 'for the next generation', attracted approximately 300 attendees from pharmaceutical industries, contractors, academic institutions, and regulatory authorities. The event provided an opportunity for young researchers in bioanalysis to discuss new frontiers and challenges in the field of pharmacokinetics.
Article
Pharmacology & Pharmacy
Kota Kato, Martin den Adel, Dorien Groenendaal-van de Meent, Yoshiaki Ohtsu, Akitsugu Takada, Masataka Katashima
CLINICAL PHARMACOLOGY IN DRUG DEVELOPMENT
(2019)
Article
Medicine, Research & Experimental
Tomohiro Kusawake, James J. Keirns, Donna Kowalski, Martin den Adel, Dorien Groenendaal-van de Meent, Akitsugu Takada, Yoshiaki Ohtsu, Masataka Katashima
ADVANCES IN THERAPY
(2017)
Article
Medicine, Research & Experimental
Tomohiro Kusawake, Martin den Adel, Dorien Groenendaal-van de Meent, Alberto Garcia-Hernandez, Akitsugu Takada, Kota Kato, Yoshiaki Ohtsu, Masataka Katashima
ADVANCES IN THERAPY
(2017)
Article
Biochemical Research Methods
Yoshiaki Ohtsu, Radboud van Trigt, Kaori Takama, Dorien Groenendaal, Akitsugu Takada, Takeshi Nakamura, Kiyoshi Noguchi
Article
Immunology
Jun Cui, Cheng Chen, Xiao Zhou, Wenju Shan, Yuhong Jian, Panpan Li, Yang Sun, Wei Yi
Summary: Bone marrow mesenchymal stem cells (BMSCs) are a promising therapy for sepsis, but metabolic syndromes threaten their effectiveness. This study investigated the potential of small extracellular vesicles from high-fat diet BMSCs in sepsis-induced liver-heart axis injury.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2024)
Article
Immunology
Binbin Zhu, Angyang Cao, Chunqu Chen, Weijian Zhou, Wenjun Luo, Yu Gui, Qinwen Wang, Zhipeng Xu, Jianhua Wang
Summary: GM6001 alleviates postoperative cognitive deficits and neuroinflammation, preserves blood-brain barrier integrity, and rescues aquaporin-4 mislocalization. MMP-9 inhibition plays a dual role in cognitive protection through direct anti-neuroinflammatory effects and regulation of aquaporin-4 membrane distribution.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2024)
Article
Immunology
Anika Sood, Valencia Fernandes, Kumari Preeti, Shruti Rajan, Dharmendra Kumar Khatri, Shashi Bala Singh
Summary: S1PR2 inhibitor improves cognitive function and skews microglia toward anti-inflammatory phenotype in type 2 diabetic mice, promising to be a potential therapy for neuroinflammation.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2024)
Article
Immunology
Haochun Guo, Ran Yu, Haijun Zhang, Wanpeng Wang
Summary: Radiation therapy is an effective treatment for thoracic malignancies, but it can cause radiation-induced lung injury (RILI), including radiation pneumonitis (RP) and radiation pulmonary fibrosis (RPF). The damage to normal lung cells during radiation treatment leads to a pulmonary inflammatory response, resulting in RP and RPF.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2024)
Article
Immunology
Guanghui Wang, Haotian Zheng, Yunzhi Xiang, Yadong Wang, Kai Wang, Xiaoyang Ren, Jiajun Du
Summary: This study identified a T-cell synthetic driver-associated prognostic model that accurately predicted prognosis and effectiveness of immunotherapy in LUAD patients. It also highlighted the role of LDHA in promoting tumor cell proliferation, invasion, and resistance to treatment, as well as its involvement in immune escape within the tumor microenvironment. These findings provide a promising new therapeutic strategy for LUAD.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2024)
Article
Immunology
Bowen Wei, Aihua Wang, Wei Liu, Qingyun Yue, Yihua Fan, Bin Xue, Siwei Wang
Summary: This study systematically analyzed the association between pSS and cuproptosis, established a predictive model based on 5 genes, explored the pathogenic mechanisms and novel therapeutic strategies for pSS, and identified EED, CBL, and NFU1 as potential targets for treatment.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2024)
Article
Immunology
Nusrit Iqbal Andrabi, Aminur R. Sarkar, Syed Assim Haq, Diljeet Kumar, Dilpreet Kour, Diksha Saroch, Sanket Kumar Shukla, Ajay Kumar, Asha Bhagat, Asif Ali, Gurleen Kour, Zabeer Ahmed
Summary: Koenimbine and its novel semi-synthetic derivative 1G demonstrate significant anti-inflammatory effects by downregulating the nuclear factor kappa-B (NF-kappa B) signaling pathway.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2024)
Article
Immunology
Jing-Mei Lu, Xiang Xu, Fumie Aosai, Ming-Yue Zhang, Lian-Xun Piao
Summary: This study found that arctiin can improve allergic acute liver injury caused by T.g.HSP70 by inhibiting TLR4 signaling and reducing the production of inflammatory mediators.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2024)
Article
Immunology
Minxuan Xu, Fang Shi, Yongshen Gao, Shumei Han, Chensuo Huang, Qinsheng Hou, Xiaoweng Wen, Bengshi Wang, Zhenyu Zhu, Lei Zou, Mingxin Xiong, Wei Dong, Jun Tan
Summary: There is a growing body of research highlighting the involvement of metabolic imbalance and the inflammatory response in the advancement of colitis. This study recognizes arabinose as a significant protector of the intestinal mucosal barrier, reducing damage to the intestines. In addition, lower levels of arabinose in the bloodstream are associated with a higher severity of inflammatory bowel disease and colorectal cancer.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2024)
Article
Immunology
Yueqing Han, Haoxin Song, Yanshan Li, Rongxin Li, Ling Chen, Bo Gao, Yijun Chen, Shuzhen Wang
Summary: The combination of tetracycline antibiotics, demeclocycline (D), chlortetracycline (C), and minocycline (M), showed therapeutic potential against liver fibrosis by inhibiting the activation of hepatic stellate cells and the MAPK signaling. This study suggests that tetracyclines may be repurposed for the treatment of liver fibrosis.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2024)
Article
Immunology
Yu Li, Hailing Liu, Danwen Zhao, Danjie Zhang
Summary: Chronic stress can lead to lung injury, with the spleen playing a crucial role. This study found that the spleen contributes to chronic restraint stress-induced lung injury, and splenic CD11b+ cells may be an important factor in this process.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2024)
Article
Immunology
Yingqian Mi, Mengyan Tang, Qiong Wu, Yinan Wang, Qihui Liu, Pei Zhu, Xiaoyang Xue, Yuntong Liu, Xinyu Chai, Yuyang Hou, Dongmei Yan
Summary: BCG therapy can induce macrophage polarization to the M1 type, and NMAAP1 plays a crucial role in this process by regulating glycolysis and HIF-1α expression. This promotes the antitumor effect of macrophages.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2024)
Article
Immunology
Xiaosheng Liu, Tingxia Lv, Xiuxia Li, Jing Xue, Ling Lin, Lianfeng Lu, Xiaodi Li, Yang Yang, Yuanni Wu, Qiang Wei, Wei Cao, Taisheng Li
Summary: LLDT-8 exhibits notable efficacy in alleviating immune activation in both an in vivo animal model and in vitro human cell experiments, suggesting its potential as a drug for managing systemic immune activation associated with SIV/HIV infection.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2024)
Article
Immunology
Honghong Yu, Qi Li, Huimin Zhu, Chang Liu, Weiwei Chen, Lingyun Sun
Summary: The activation of the inflammasome plays a critical role in the pathogenesis of systemic lupus erythematosus (SLE), and mesenchymal stem cells (MSC) have been shown to alleviate SLE by suppressing inflammasome activation. This study found that the NLRP3 inflammasome was activated in macrophages from SLE patients and mice, and its activation correlated with disease activity. After MSC transplantation, the severity of SLE was reduced, and NLRP3 inflammasome activation was inhibited. These findings suggest that MSC suppress inflammasome activation and provide a potential therapeutic target for SLE.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2024)
Article
Immunology
Wei Zhou, Dan Zeng, Shunan Liu, Yunxia Huang, Fenglin Lv, Weikang Zhou
Summary: This study found that inhibiting HDAC3 can protect the skin from atopic dermatitis by activating the Nrf2 transcription to upregulate Nrf2/HO-1 signaling pathway activity.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2024)