Article
Cardiac & Cardiovascular Systems
Asthildur Arnadottir, Sune Pedersen, Rasmus Bo Hasselbalch, Jens P. Goetze, Lennart J. Friis-Hansen, Anna-Marie Bloch-Munster, Jan Skov Jensen, Henning Bundgaard, Kasper Iversen
Summary: This study is the first to report the early-release kinetics of cTn concentrations after different durations of experimental coronary balloon occlusion in humans. All assays detected a cTn increase after only 30 s of ischemia. hs-cTnI (Siemens) rose faster and reached a higher peak. Copeptin levels did not change significantly.
Article
Engineering, Electrical & Electronic
Rajeev K. Sinha
Summary: A wavelength modulation based surface plasmon resonance sensor setup was developed for the detection of cardiac marker proteins, CTnI and CTnT, with a simple approach used for the fabrication of the CTnI/CTnT antibody sensor chip. The developed setup and sensor chip showed good performance for the detection of CTnI and CTnT in direct assay, with detected concentrations well below the onset concentration of these proteins in blood following AMI, allowing for easy detection during their onset period.
SENSORS AND ACTUATORS A-PHYSICAL
(2021)
Article
Cell Biology
Fernanda O. Lemos, Ian de Ridder, Martin D. Bootman, Geert Bultynck, Jan B. Parys
Summary: PKM2 regulates calcium handling in the cytosol and mitochondria separately, while TAT-D5SD induces apoptosis by disrupting the PKM2:IP3R interaction.
Article
Cell Biology
Giulia Rigotto, Lorena Zentilin, Tullio Pozzan, Emy Basso
Summary: The study on the role of mitochondria and Ca2+ signaling in a Familial Alzheimer's disease model revealed early alterations in mitochondria physiology under stress conditions, such as reduced maximal respiration, decreased ability to sustain membrane potential, and a slower return to basal matrix Ca2+ levels after mild excitotoxic stimulus. Treatment with an inhibitor of the permeability transition pore attenuated some of these mitochondrial dysfunctions, showing promise in ameliorating mitochondrial and cellular functioning in AD to prevent or slow down cell loss in the disease.
Article
Sport Sciences
Daniel Khai Nguyen, Oyvind Ellingsen, Bjornar Grenne, Thomas Fremo, Gunhild Garmo Hov, Ragnhild Rosbjorgen, Gustav Mikkelsen
Summary: This study aimed to determine whether exercise at moderate intensity would significantly increase plasma cardiac troponin concentrations in healthy athletes. The results showed that 60 minutes of high-intensity training led to a significant increase in plasma cardiac troponins, while moderate- and low-intensity training did not cause significant changes.
SCANDINAVIAN JOURNAL OF MEDICINE & SCIENCE IN SPORTS
(2023)
Review
Biochemistry & Molecular Biology
Antonio Michelucci, Chen Liang, Feliciano Protasi, Robert T. Dirksen
Summary: This review highlights the importance of Ca2+ signaling and ATP generation in skeletal muscle contraction and outlines the role of impaired Ca2+ handling, dysfunctional ROS/RNS production, and structural/functional alterations in CRUs and mitochondria in various muscle disorders. Understanding these processes is crucial for identifying potential therapeutic targets to prevent or reverse muscle dysfunction in aging and disease.
Article
Biochemistry & Molecular Biology
Bracy Fertig, Jiayue Ling, Edgar E. Nollet, Sara Dobi, Tara Busiau, Kiyotake Ishikawa, Kelly Yamada, Ahyoung Lee, Changwon Kho, Lauren Wills, Amy J. Tibbo, Mark Scott, Kirsten Grant, Kenneth S. Campbell, Emma J. Birks, Niall MacQuaide, Roger Hajjar, Godfrey L. Smith, Jolanda van Der Velden, George S. Baillie
Summary: This study reports for the first time that human troponin I can be modified by SUMOylation at lysine 177, which is upregulated during heart disease and has an indirect impact on calcium sensitivity and cooperativity of myofilaments.
Article
Biochemistry & Molecular Biology
Veronica Costiniti, Guilherme H. S. Bomfim, Maria Neginskaya, Ga-Yeon Son, Erna Mitaishvili, Marta Giacomello, Evgeny Pavlov, Rodrigo S. Lacruz
Summary: This study analyzed the functional features of mitochondria in rat ameloblasts and found an increased mitochondrial Ca2+ handling in the maturation stage, particularly in response to Ca2+ influx via store-operated Ca2+ entry (SOCE). These findings provide insights into the role of mitochondria in enamel and suggest that SOCE and mitochondrial Ca2+ uptake are complementary processes in biological mineralization.
Article
Cardiac & Cardiovascular Systems
Franz Masseli, Marc Masseli, Ramona Dolscheid-Pommerich, Birgit Stoffel-Wagner, Wolfgang Schiller
Summary: This study compared the performance of high-sensitive troponin T (hsTnT) assay and troponin I (sTnI) assay in the perioperative period of cardiac surgery. Despite significant differences in absolute values, both assays can be scaled to similar distributions. However, renal impairment affects the parameters differently, with troponin T showing evidence of accumulation in moderately impaired renal disease.
HEART LUNG AND CIRCULATION
(2022)
Review
Biochemistry & Molecular Biology
Feliciano Protasi, Laura Pietrangelo, Simona Boncompagni
Summary: This passage emphasizes the importance of proper Ca2+ concentration and ATP production for skeletal muscle function, while highlighting that abnormalities in Ca2+ handling underlie many physiological conditions. It discusses the significance of organelle and membrane system architecture for correct muscle function and presents findings on structural changes caused by denervation and aging which impact muscle dysfunction.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Neurosciences
Xianwei Zhang, Charlotte E. R. Smith, Stefano Morotti, Andrew G. Edwards, Daisuke Sato, William E. Louch, Haibo Ni, Eleonora Grandi
Summary: This study investigates the independent and interactive effects of TATS and Ca2+-handling protein remodelling on Ca2+-handling abnormality driven membrane instabilities. The results show that the impact of protein remodelling on Ca2+-driven proarrhythmic behaviour varies depending on TATS density.
JOURNAL OF PHYSIOLOGY-LONDON
(2023)
Review
Pharmacology & Pharmacy
Tiago Rodrigues, Silvia Piccirillo, Simona Magi, Alessandra Preziuso, Vyctoria dos Santos Ramos, Tiziano Serfilippi, Monia Orciani, Marcela Maciel Palacio Alvarez, Ivarne Luis dos Santos Tersariol, Salvatore Amoroso, Vincenzo Lariccia
Summary: Spatial and temporal control of calcium levels is crucial for cell activities. Na+/Ca2+ exchangers (NCXs) play a role in maintaining calcium homeostasis and supporting cellular metabolism. Preserving a timed and balanced NCX activity has therapeutic potential for various diseases.
BIOCHEMICAL PHARMACOLOGY
(2022)
Article
Medical Laboratory Technology
Leo Lam, Leah Ha, Campbell Heron, Weldon Chiu, Campbell Kyle
Summary: The study identified four cases of macro-cTnT with persistently low hs-cTnT levels, characterized by differences in recovery with mixing studies using recombinant cTnT or EDTA. One patient demonstrated a high molecular weight complex involving both cTnI and cTnT, showing consistent results of acute cardiac injury across multiple commercial assays.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2021)
Review
Medical Laboratory Technology
Julien Favresse, Jean-Louis Bayart, Damien Gruson, Sergio Bernardini, Aldo Clerico, Marco Perrone
Summary: Cardiac troponins play a key role in diagnosing MI, but interference can lead to misdiagnosis. False positive and false negative results are related to antibody interference and biotin intake, with non-reproducible results and outliers also reported.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2021)
Article
Oncology
Edmond Ang, Angela Mweempwa, Campbell Heron, Yeri Ahn, Gareth Rivalland, Leah Y. Ha, Sanjeev Deva
Summary: Checkpoint inhibitor-associated myocarditis and myositis may occur concurrently, and cardiac-specific troponin I may be a more appropriate biomarker for diagnosing and monitoring myocarditis in the context of concurrent myositis.
JOURNAL OF IMMUNOTHERAPY
(2021)
Article
Oncology
Wubulikasimu Wulamu, Maimaiti Yisireyili, Aikebaier Aili, Kyosuke Takeshita, Aziguli Alimujiang, Aliyeguli Aipire, Yiliang Li, Yuan Jiang, Maimaitiaili Aizezi, Zanlin Li, Kelimu Abudureyimu
MOLECULAR MEDICINE REPORTS
(2019)
Editorial Material
Gastroenterology & Hepatology
Kyosuke Takeshita
DIGESTIVE DISEASES AND SCIENCES
(2020)
Article
Multidisciplinary Sciences
Satoshi Nishiwaki, Shigeki Saito, Kyosuke Takeshita, Hidefumi Kato, Ryuzo Ueda, Akiyoshi Takami, Tomoki Naoe, Mika Ogawa, Takayuki Nakayama
Article
Biochemical Research Methods
Makoto Sawano, Kyousuke Takeshita, Hideaki Ohno, Hideaki Oka
Summary: The study aimed to quantify the viral load in exhaled breath condensate (EBC) collected from COVID-19 patients and validate the feasibility of using EBC for SARS-CoV-2 detection as a diagnostic test for the infection. Results showed that the need for mechanical ventilation was associated with higher viral load, while the need for oxygen administration or mechanical ventilation, less than 3 days since onset, and presence of cough or fever were significantly associated with higher detection rates.
JOURNAL OF BREATH RESEARCH
(2021)
Article
Multidisciplinary Sciences
Kazutoshi Yamaguchi, Motoharu Hayashi, Yasuhiro Uchida, Xian Wu Cheng, Takayuki Nakayama, Tadashi Matsushita, Toyoaki Murohara, Kyosuke Takeshita
Summary: Haploinsufficiency of Notch1 promotes fat accumulation and adipogenesis, resulting in impaired glucose and insulin tolerance in N1+/- mice after high-fat diet, with downregulation of IRS-1 and GLUT4 in WAT.
SCIENTIFIC REPORTS
(2021)
Article
Cardiac & Cardiovascular Systems
Kazutoshi Yamaguchi, Maimaiti Yisireyili, Sumie Goto, Xian Wu Cheng, Takayuki Nakayama, Tadashi Matsushita, Toshimitsu Niwa, Toyoaki Murohara, Kyosuke Takeshita
Summary: In patients with chronic kidney diseases, high levels of serum indoxyl sulfate (IS) are strongly associated with cardiac fibrosis and hypertrophy, which are critical risk factors for heart failure. This study found that IS can induce cardiac fibrosis and hypertrophy, impair left ventricular function, and activate the NLRP3 inflammasome pathway via the AHR/NF-kappa B pathway.
CARDIOVASCULAR TOXICOLOGY
(2022)
Article
Biochemical Research Methods
Makoto Sawano, Kyousuke Takeshita, Hideaki Ohno, Hideaki Oka
Summary: The study aims to investigate the temporal changes in RNA load and detection rates of Delta variant SARS-CoV-2 in exhaled breath condensate (EBC) from COVID-19 patients, as well as validate the feasibility of using EBC for diagnosing COVID-19. The results demonstrate a exponential decrease in SARS-CoV-2 RNA load in EBC collected from Delta variant-infected individuals over time, while the detection rates remained high.
JOURNAL OF BREATH RESEARCH
(2022)
Article
Medicine, General & Internal
Daisuke Ono, Yuko Ohno, Yoshihiko Izumida, Hideaki Ohno, Hideaki Oka, Kyosuke Takeshita
Summary: Inflammation may serve as a suitable marker and target for prophylaxis against COVID-19-related thrombosis.
INTERNATIONAL JOURNAL OF MEDICAL SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Mizuho Suzuki, Kyosuke Takeshita, Yuki Kitamura, Marie Kuribayashi, Zhenlie Huang, Gaku Ichihara, Shinji Oikawa, Sahoko Ichihara
Summary: Diabetes mellitus is a pro-thrombotic state that can potentially cause serious cardiovascular complications. Platelet hyperactivation plays an important role in these pathological processes, however there is little or no information on the effect of hyperglycemia on platelet proteins. The aim of this study was to identify the molecular targets associated with platelet reactivity under hyperglycemia.
Article
Biology
Siqiang Gao, Mika Ogawa, Akiyoshi Takami, Kyosuke Takeshita, Hidefumi Kato, Takayuki Nakayama
Article
Medicine, General & Internal
Kazutoshi Yamaguchi, Maimaiti Yisireyili, Sumie Goto, Katsuhiro Kato, Xian Wu Cheng, Takayuki Nakayama, Tadashi Matsushita, Toshimitsu Niwa, Toyoaki Murohara, Kyosuke Takeshita
INTERNATIONAL JOURNAL OF MEDICAL SCIENCES
(2020)
Article
Biochemical Research Methods
Makoto Sawano, Kyousuke Takeshita, Hideaki Ohno, Hideaki Oka
JOURNAL OF BREATH RESEARCH
(2020)
Article
Medicine, General & Internal
Kenji Furusawa, Kyosuke Takeshita, Susumu Suzuki, Yosuke Tatami, Ryota Morimoto, Takahiro Okumura, Yoshinari Yasuda, Toyoaki Murohara
INTERNATIONAL JOURNAL OF MEDICAL SCIENCES
(2019)
Article
Radiology, Nuclear Medicine & Medical Imaging
Yutaka Sasaki, Yoshiki Hirooka, Hiroki Kawashima, Takuya Ishikawa, Kyosuke Takeshita, Hidemi Goto
Article
Medicine, Research & Experimental
Mohammad Shoaib Hamrah, Mohammad Hassan Hamrah, Hideki Ishii, Susumu Suzuki, Mohammad Hussain Hamrah, Ahmad Edris Hamrah, Ahmad Elias Dahi, Maimaiti Yisireyili, Naoaki Kano, Kyosuke Takeshita, Mohammad Hashem Hamrah, Junichi Sakamoto, Toyoaki Murohara
NAGOYA JOURNAL OF MEDICAL SCIENCE
(2018)