Article
Chemistry, Multidisciplinary
Anna Milewska-Hendel, Weronika Gepfert, Maciej Zubko, Ewa Kurczynska
Summary: Gold nanoparticles had a negative impact on barley root development, causing morphological, histological, and ultrastructural changes.
APPLIED SCIENCES-BASEL
(2022)
Article
Biotechnology & Applied Microbiology
Zhourui Liang, Xiao Wang, Pengyan Zhang, Wenhui Liu, Wenjun Wang, Fuli Liu
Summary: The processes and mechanisms of gametogenesis in multiannual delayed gametophytes of kelp Saccharina japonica were investigated in this study. Results showed notable changes in ultrastructure, increased contents of abscisic acid and H2O2 as well as activities of respiratory and antioxidant enzymes during gametogenesis induction. Photosynthetic and respiratory indicators were upregulated after 6 days of induction, suggesting increased metabolic activity. Time-specific differentially expressed genes (DEGs) were identified, with cell part, cellular process, and binding being the major categories. Transcription factor families bHLH, MYB, and C3H were predominant in the DEG datasets. Several genes related to peroxisomes, proteasomes, oxidative phosphorylation, and ubiquitin-mediated proteolysis were identified as DEGs at key stages of gametogenesis.
JOURNAL OF APPLIED PHYCOLOGY
(2023)
Article
Toxicology
Piotr Strus, Karol Borensztejn, Andrzej Antoni Szczepankiewicz, Kamil Lisiecki, Zbigniew Czarnocki, Hanna Nieznanska, Cezary Wojcik, Lukasz P. Bialy, Izabela Mlynarczuk-Bialy
Summary: The KL3 derivative of PPT is less toxic to non-cancerous cells compared to PPT, allowing for repair of normal cells and triggering mechanisms to restore cell homeostasis.
TOXICOLOGY IN VITRO
(2021)
Article
Biochemistry & Molecular Biology
Marcello Trucas, Sabrina Burattini, Susanna Porcu, Michela Simbula, Maria Serafina Ristaldi, Marta Anna Kowalik, Maria Pina Serra, Pietro Gobbi, Michela Battistelli, Andrea Perra, Marina Quartu
Summary: Sickle cell disease (SCD) is caused by a homozygous beta-globin gene mutation and can result in multi-organ damage and reduced life expectancy. Sickle cell trait (SCT), the heterozygous beta-globin gene mutation, has been considered benign, but recent clinical evidence suggests otherwise. This study used a mouse model to investigate tissue alterations in SCT carriers and found endothelial alterations in multiple organs, with the lung showing the most significant changes. These findings provide new insights into the histopathological modifications associated with the heterozygous beta-globin gene mutation and highlight the suitability of the Townes model for studying multiple organ involvement in SCT carriers.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Sameerah Al-Saeedi, Nada S. Al-Kadhi, Ghadah M. Al-Senani, Omar A. Almaghrabi, Ayman Nafady
Summary: The study successfully prepared scaffold-based cellulose acetate loaded with copper nanoparticles, which improved the hydrophilicity and antimicrobial properties of the nanofibers. The addition of CuONPs resulted in changes in roughness and contact angle of the nanofibers, as well as enhanced cell viability and antibacterial potency.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Engineering, Environmental
Xi Li, Weijun Song, Xuanxuan Xian, Chuchu Zhang, Shengda Wang
Summary: This study investigates the effect of cell viability on the efficiency of PACl coagulation. The results show that the removal efficiency of low-viability cells is significantly higher than that of high-viability cells after coagulation-sedimentation. The removal efficiency of low-viability cells is influenced by cellular properties and extracellular organic matter.
JOURNAL OF WATER PROCESS ENGINEERING
(2023)
Article
Microbiology
Tanapon Soonthonsrima, Htut Htut Htoo, Parameth Thiennimitr, Veerasak Srisuknimit, Poochit Nonejuie, Vorrapon Chaikeeratisak
Summary: In a post-antibiotic era, finding antibiotic alternatives has become crucial in fighting against pathogens. This study investigated phage-induced bacterial morphological changes and discovered a potential phage-derived antimicrobial embedded in the phage genome. By analyzing the genome, lytic transglycosylase (LT) was identified as a gene product of interest for further studies. The heterologous expression of wild-type KVP40-LT induced similar bacterial morphological changes to those caused by the phage or cell wall synthesis inhibitors, while truncated or catalytic mutant KVP40-LT had no effect, suggesting the importance of these domains for antibacterial activities.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Environmental Sciences
Peng Yan, Jin-song Guo, Ping Zhang, Yan Xiao, Zhe Li, Shu-qing Zhang, Yu-xin Zhang, Shi-xuan He
Summary: Individual cell heterogeneity within algal populations plays a critical role in their unique function and fate. Algal cells may change their morphology in response to environmental stress, modulating their physiology and metabolism. Different species of algae adopt different adaptation strategies based on morphological changes in response to nutrient stress.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Oncology
Takuya Nishinakagawa, Mai Hazekawa, Masato Hosokawa, Daisuke Ishibashi
Summary: RCAS1 is a tumor-associated antigen expressed in various human malignancies which acts as a ligand for a putative receptor on immune cells, inhibiting their growth and inducing apoptosis. Its expression is associated with malignant characteristics and poor overall survival. This study examined the signaling pathways involved in the cell morphological changes induced by RCAS1 expression, and found that it decreased actin stress fibers and phosphorylation of cofilin through the inhibition of p38 signaling.
MOLECULAR MEDICINE REPORTS
(2023)
Article
Microbiology
Samantha Reese, Cynthia Chelius, Wayne Riekhof, Mark R. Marten, Steven D. Harris
Summary: Fungal cell wall receptors communicate the state of the cell wall to the nucleus through the Cell Wall Integrity Signaling (CWIS) pathway, which coordinates cell wall damage repair and restores normal hyphal growth. Echinocandins like micafungin trigger cell wall damage by impacting beta-glucan synthesis, leading to induction of the master regulator BrlA and morphological changes consistent with microcycle conidiation in response to micafungin exposure, demonstrating a potential survival mechanism against lethal damage.
Article
Chemistry, Multidisciplinary
Shaidatul Najihah Matussin, Fazlurrahman Khan, Pathum Chandika, Mohammad Hilni Harunsani, Norhayati Ahmad, Young-Mog Kim, Won-Kyo Jung, Mohammad Mansoob Khan
Summary: CeO2 nanoparticles were synthesized using a modified polyol method, and their structure, size, and morphology were studied. Varying ratios of diethylene glycol (DEG) and water resulted in different particle sizes. The synthesized CeO2 nanoparticles showed good antidiabetic activity and biocompatibility. CeO2 prepared with Ce(NO3)(3) and CeCl3 precursors exhibited higher enzyme inhibition activity.
Article
Microscopy
Raghda Elsherif, Waleed Ahmed Mahmoud, Refaat R. Mohamed
Summary: Vitiligo is a chronic pigmentary disorder characterized by depigmented macules and patches. This study aimed to evaluate the morphological changes of melanocytes and keratinocytes in patients with vitiligo. The findings showed various degenerative changes in keratinocytes and melanocytes in the lesioned skin of vitiligo patients.
ULTRASTRUCTURAL PATHOLOGY
(2022)
Article
Multidisciplinary Sciences
Jeong Pyo Son, Ji Hee Sung, Dong Hee Kim, Yeon Hee Cho, Suk Jae Kim, Jong-Won Chung, Won Hyuk Chang, Yun-Hee Kim, Eun Hee Kim, Gyeong Joon Moon, Oh Young Bang
Summary: Behavioral assessments in animal models of stroke could be complemented by neuroimaging studies to better understand mechanisms of improvement after stem cell therapy. Morphological and connectivity changes were evaluated in a rat stroke model after treatment with human mesenchymal stem cells (hMSCs), showing the most prominent functional improvement in the group treated with stroke patients' serum cultured hMSCs, which correlated with T-2-weighted morphological changes.
Article
Orthopedics
Mathilde Padrona, Manon Maroquenne, Hanane El-Hafci, Lucille Rossiaud, Herve Petite, Esther Potier
Summary: This study evaluated the effects of glucose depletion on bovine nucleus pulposus explant homeostasis using a relevant ex vivo model representative of the unique intervertebral disc microenvironment. Exposure to low glucose conditions led to a significant decrease in cell viability but had minimal effects on cell phenotype, senescence, matrix catabolism, and inflammatory mediator production.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2023)
Article
Plant Sciences
Kamila Godel-Jedrychowska, Ewa Kurczynska
Summary: Plasmodesmata play a crucial role in symplasmic communication in plants, regulating cell differentiation. The study focused on describing the qualitative and quantitative analyses of PD, highlighting their importance in symplasmic communication during somatic embryogenesis. Differences in PD shape and distribution were observed between symplasmic domains within and between Col-0 and 35S:BBM somatic embryos.
FUNCTIONAL PLANT BIOLOGY
(2022)
Article
Pharmacology & Pharmacy
Andrea Barrios-Gumiel, Javier Sanchez-Nieves, Elzbieta Pedziwiatr-Werbicka, Viktar Abashkin, Natallia Shcharbina, Dzmitry Shcharbin, Slawa Glinska, Karol Ciepluch, Dorota Kuc-Ciepluch, Dominika Lach, Maria Bryszewska, Rafael Gomez, F. Javier de la Mata
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2020)
Article
Environmental Sciences
Julia Mironenka, Sylwia Rozalska, Adrian Sobon, Przemyslaw Bernat
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2020)
Article
Environmental Sciences
Rafal Szewczyk, Sylwia Rozalska, Julia Mironenka, Przemyslaw Bernat
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2020)
Article
Cell Biology
Paulina Zelechowska, Ewa Brzezinska-Blaszczyk, Sylwia Rozalska, Justyna Agier, Elzbieta Kozlowska
Summary: The study found that fungal mannan can activate mast cells to produce various anti-fungal responses mediated through TLR4, affecting the course of the immune response.
JOURNAL OF LEUKOCYTE BIOLOGY
(2021)
Article
Immunology
Paulina Zelechowska, Ewa Brzezinska-Blaszczyk, Sylwia Rozalska, Justyna Agier, Elzbieta Kozlowska
Summary: The research demonstrates that Saccharomyces cerevisiae-derived zymosan can induce pro-inflammatory responses and migration of mast cells, potentially affecting innate antifungal immunity. Zymosan was found to stimulate mast cells to produce inflammatory mediators and chemokines, with the response mediated through activation of the Dectin-1 receptor.
IMMUNOLOGIC RESEARCH
(2021)
Article
Microbiology
Julia Mironenka, Sylwia Rozalska, Adrian Sobon, Przemyslaw Bernat
Summary: Trichoderma species are known for producing secondary metabolites in response to different fungal pathogens, with the metabolites showing inhibitory effects on the growth of popular pathogen Fusarium culmorum. The presence of certain proteins in the culture extract indicated oxidative stress, leading to activation of defense mechanisms in the pathogen and a reduction in major mycotoxin production.
MICROBIOLOGICAL RESEARCH
(2021)
Article
Cell Biology
Justyna Agier, Ewa Brzezinska-Blasczyka, Sylwia Rozalska, Magdalena Wiktorska, Elzbieta Kozlowka, Paulina Zelechowska
Summary: The study reveals that alarmins CRAMP and IL-33 significantly impact the phenotype and activity of MCs, promoting inflammatory responses and controlling the activity of cells involved in antimicrobial processes.
CELLULAR IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Julia Mironenka, Sylwia Rozalska, Przemyslaw Bernat
Summary: The application of the fungus Trichoderma harzianum in germinating wheat seeds can alleviate oxidative stress induced by 2,4-D or Fusarium culmorum.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Anna Litwin, Przemyslaw Bernat, Monika Nowak, Miroslawa Slaba, Sylwia Rozalska
Summary: Pyrethroids can change the phospholipid profile and permeability of Beauveria bassiana's cell membrane, leading to oxidative stress. This interaction may have a more complex effect on entomopathogenic fungi than previously thought, and lipidomic studies can help in fully understanding their influence.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Aleksandra Goralczyk-Binkowska, Andrzej Dlugonski, Przemyslaw Bernat, Jerzy Dlugonski, Anna Jasinska
Summary: The study found that chemicals such as dyes, heavy metals and aromatic amines present in urban textile industry wastewater can promote the growth of fungal biomass, with the fungus N. pironii showing good degradation and adsorption capabilities for these compounds.
SCIENTIFIC REPORTS
(2021)
Article
Cell Biology
Elzbieta Skiba, Monika Pietrzak, Slawa Glinska, Wojciech M. Wolf
Summary: Cerium oxide nanoparticles and zinc oxide nanoparticles, as emerging pollutants, have not been thoroughly studied for their combined effects on terrestrial plants like Pisum sativum L. The study shows that the free radicals scavenging properties of cerium oxide nanoparticles alleviate the toxicity symptoms induced in plants by zinc oxide nanoparticles.
Article
Horticulture
Waldemar Kiszczak, Urszula Kowalska, Maria Burian, Slawa Glinska, Marcin Domaciuk, Krystyna Gorecka
Summary: Yacon micropropagation was optimized in this study. The most effective disinfection method was using 70% ethanol and 0.5% Tween 20. The optimal initial explants were apical and lateral buds cultured on Murashige and Skoog (MS) medium with 0.2 mg L-1 kinetin and 1 mg L-1 indole-3-acetic acid. The most intensive shoot regeneration occurred on MS medium with 1 mg L-1 thidiazuron and 1-naphthylacetic acid. Shoots obtained from in vitro cultures rooted easily on modified MS media with reduced macroelements and supplemented with sucrose and 1-naphthylacetic acid.
ACTA SCIENTIARUM POLONORUM-HORTORUM CULTUS
(2022)
Article
Environmental Sciences
Anna Litwin, Julia Mironenka, Przemyslaw Bernat, Adrian Sobon, Sylwia Rozalska
Summary: Advancements in agrochemical industry lead to environmental pollution due to the accumulation of toxic compounds in soil. This study found that the soil-dwelling entomopathogenic fungus Beauveria bassiana ARSEF 2860 is capable of accumulating pyrethroids and identified the metabolic and proteomic implications of this process.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Biotechnology & Applied Microbiology
Monika Nowak, Adrian Sobon, Przemyslaw Bernat, Sylwia Rozalska
Summary: In this study, the ability of the biocontrol agent Cordyceps to eliminate Zearalenone (ZEN) was evaluated. Through various reactions, 19 metabolites of ZEN biotransformation were detected, including new metabolites formed by Cordyceps. Enzymes responsible for oxidation, sulfonation, glycosylation, and reduction were determined. Furthermore, ZEN was found to reduce beauvericin levels in Cordyceps, potentially reducing environmental contamination with this compound.
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
(2023)
Article
Biochemistry & Molecular Biology
Anna Jasinska, Adrian Sobon, Sylwia Rozalska, Paulina Srednicka
Summary: The fungus M. roridumIM 6482 can effectively remove BPA from the environment, with extracellular laccase activity identified as the main mechanism. The study found that BPA induces oxidative stress in fungal cells, which is alleviated after BPA degradation. Proteome analysis revealed the involvement of various proteins, including oxidoreductive and antioxidative agents, in the biodegradation process of BPA.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Shaofeng Zhou, Wenwen An, Cuifen Gan, Meiying Xu
Summary: Bacteria generate and release extracellular reactive oxygen species (eROS), which significantly contribute to the natural ROS pool and play a crucial role in metabolic interactions of bacteria with their environment. Understanding the behavior of eROS-generating bacteria and its environmental implications is of great importance in the cycling of biogeochemical elements, transformation of organic matter, and regulation of antibiotic resistance genes.
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
(2024)
Article
Biotechnology & Applied Microbiology
Ji-Dong Gu, Tim Ford, Bryce Mitton, Ralph Mitchell
Summary: Plastics and microplastics are widely present and accumulating in the environment, leading to increasing public attention. In addition to packaging plastics, materials from industries such as electronics, space and aviation are also accumulating, but receiving less attention. The production of degradable and biodegradable plastics is seen as a way to reduce environmental impacts, but enhancing the non-degradability of functional polymers is also a feasible option. Microbial colonization and deterioration pose significant challenges to these materials.
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
(2024)
Article
Biotechnology & Applied Microbiology
Justyna Bohacz, Michal Mozejko
Summary: This study evaluated the keratinolytic activity of 37 strains of Trichophyton ajelloi and found that pigmented strains isolated from loamy soil were more efficient in degrading native feather keratin, while non-pigmented strains isolated from chernozem showed better abilities. The keratinolytic activity increased over time, as evidenced by the increase in extracellular keratinase activity and release of soluble proteins and peptides. Protease activity peaked in the first 7 days and again on day 28 of culture. The release of ammonium and sulfate ions, associated with an increase in pH, reached its maximum on day 21. Three pigment-producing strains were among the most active in releasing large amounts of ammonium and sulfate ions. The biodegradation of feather waste by Trichophyton ajelloi strains can be used to produce fertilizers suitable for plants with high sulfur requirements.
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
(2024)
Article
Biotechnology & Applied Microbiology
Joseph M. Suflita, Brenda J. Little
Summary: This article reviews the major achievements of the International Biodeterioration & Biodegradation Society (IBBS) on its 50th anniversary and provides recommendations for addressing new challenges and developments based on personal experiences.
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
(2024)
Article
Biotechnology & Applied Microbiology
Zhenyan Lin, Tuo Jin, Xin Xu, Xiao Yin, Dan Zhang, Mengjiao Geng, Chunyu Pang, Gongwen Luo, Lizhi Xiong, Jianwei Peng, Jiangchi Fei
Summary: The incomplete recycle of residual mulch film (RMF) in recent years has led to an increase in plastic fragments in soil, posing a serious threat to the soil ecological environment. In this study, seven potential plastic-degrading microorganisms were screened and identified from film-mulched vegetable fields. The degradation ability experiments showed that both single strains and mixed strains demonstrated varying levels of degradation of RMF.
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
(2024)
Article
Biotechnology & Applied Microbiology
Tiina Belt, Michael Altgen, Muhammad Awais, Martin Nopens, Lauri Rautkari
Summary: This study investigated the degradation of heat-treated wood by brown rot fungi and found that the decay increased the hygroscopicity of wood in the decaying state. Furthermore, it was observed that heat-treated samples were more susceptible to fungal decay compared to untreated samples, which may be related to the increase in moisture content.
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
(2024)
Article
Biotechnology & Applied Microbiology
Marta Cicardi, Davide Bernasconi, Luca Martire, Linda Pastero, Giulia Caneva, Sergio E. Favero-Longo
Summary: This study investigated the phenomenon of Centimetric circular areas Uncolonized by Microbial Biofilms (CUMBs) on natural and heritage stone surfaces. The analysis revealed a compatibility in distribution and size between CUMBs and lichen thalli, suggesting a lichen origin for some CUMBs. Microscopic analysis also showed similar modifications in marble layers beneath CUMBs and lichens, indicating a possible alteration of stone properties by lichens.
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
(2024)
