Journal
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
Volume 87, Issue -, Pages 52-59Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.ibiod.2013.11.005
Keywords
Acinetobacter; Alkane hydroxylase gene; Crude oil; Degradation; Thermal effect
Funding
- National Outstanding Youth Research Foundation of China [40925010]
- National Natural Science Foundation of China [40920134003, 41273092, 41103060, 41103058]
- Chinese Ministry of Science and Technology [2010DFB23160]
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Isolating novel crude-oil-degrading bacteria from oil-water mixture of oil production well and evaluating their degradation capacities are vitally important in the remediation of oil-polluted environments and crude oil exploitation. According to the molecular screening with degenerate primers of alkane hydroxylase gene (alk B), a strain Acinetobacter sp. LS-1 with alk B gene was isolated. This strain exhibited a 99.9% similarity with genus Acinetobacter. This alk B gene which is one of the key enzymes of metabolic process was identified. This gene sequence showed 98% similarity of its nucleotide and related amino acids to the genus Marinobacter but exhibited below 70% similarity to the genus Acinetobacter. This phylogenetic analysis indicated that alk B may have been transferred horizontally between bacteria. The isolated strain could utilize crude oil as the sole carbon, achieving a high degradation (70.3%) in 7 days. Microcalorimetric analysis of the metabolic processes for hexadecane degradation also demonstrated the ability of this strain to utilize hydrocarbons. Thus, this strain enables to degrade hydrocarbons as the sole carbon source from the gene level, combined with material and energy metabolism. These findings will benefit this strain in the remediation of oil-polluted environments and oil exploitation. (C) 2013 Elsevier Ltd. All rights reserved.
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