Article
Fisheries
Ya Tang, Yao-Wen Hu, Shao-Hua Wang, Min Zhou, Yan-Juan Ding, Si-qi Cai, Bin Tang, Shi-Gui Wang
Summary: To investigate the role of CrebA in the reproduction and immune regulation of L. migratoria, its expression and effects through RNA interference were studied. CrebA was widely expressed in various tissues of adult locusts, especially in the female fat body. Silencing of CrebA inhibited ovarian development and downregulated key reproduction gene expressions. Furthermore, CrebA silencing resulted in higher mortality rates and changes in antimicrobial peptide gene expressions and enzyme activities in locusts infected by bacteria.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2023)
Article
Agronomy
Xuekai Shi, Xiaojian Liu, Anastasia Mw Cooper, Kristopher Silver, Hans Merzendorfer, Kun Yan Zhu, Jianzhen Zhang
Summary: This study identified and characterized the V-ATPase-c gene in Locusta migratoria and found that it is primarily expressed in the Malpighian tubules. The silencing of LmV-ATPase-c resulted in severe molting defects and impaired RNA interference efficiency in the insect. These findings suggest that LmV-ATPase-c plays a crucial role in cuticle formation and dsRNA escape.
PEST MANAGEMENT SCIENCE
(2022)
Article
Entomology
Lu Gao, Yanli Wang, Mureed Abbas, Tingting Zhang, Enbo Ma, Shuping Xing, Kun Yan Zhu, Jianzhen Zhang
Summary: The study identified an LmR2D2 gene in Locusta migratoria, encoding a protein with two dsRNA-binding domains. LmR2D2 plays an important role in the siRNA pathway, and its expression levels are stable during development in Locusta migratoria, with higher levels in the testis and ovary. Silencing LmR2D2 led to reduced RNAi efficiency against a marker gene, supporting its essential role in siRNA binding in vitro.
Article
Biotechnology & Applied Microbiology
Hongsen Zhang, Zhenya Li, Hongfei Zhang, Yan Li, Fengqin Wang, Hui Xie, Lijuan Su, Andong Song
Summary: This study revealed the mechanisms of biodegradation of gramineous lignocellulose in Locusta migratoria manilensis, emphasizing that the foregut of the locust was the main degradation area, while the midgut played a crucial role in nutrient absorption. These findings provide valuable insights for the development of enzymatic pretreatment processes mimicking the locust digestive system for converting lignocellulosic biomass to fuels and chemicals.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Agronomy
Junya Wang, Hongmei Li, Yumeng Cheng, Guangjun Wang, Xiangqun Nong, Belinda Luke, Undarmaa Davaasambuu, Guocai Zhang
Summary: This study found that feeding with dsSerpin1 can enhance the infection of pests by M. anisopliae, and the effect is dose-dependent. 20μg is an effective dosage to enhance the insecticidal efficiency of M. anisopliae.
Article
Agronomy
Beltran Ortola, Alberto Urbaneja, Marcelo Eiras, Meritxell Perez-Hedo, Jose-Antonio Daros
Summary: The study demonstrates the potential of dsRNA molecules as oral insecticides in medfly.
PEST MANAGEMENT SCIENCE
(2023)
Article
Entomology
Beibei Li, Hongmei Li, Ye Tian, Nazir Ahmed Abro, Xiangqun Nong, Zehua Zhang, Guangjun Wang
Summary: The study demonstrated the crucial role of Lmserpin1 gene in the humoral immunity of insects, enhancing immune responses in Locusta migratoria manilensis and inhibiting infection by Metarhizium anisopliae.
Article
Biochemistry & Molecular Biology
Xiaohong Zhang, Zhixing Liu, Mengdi Li, Ziyang Zhang, Xiaochen Wen, Daochuan Zhang, Hong Yin
Summary: The transcription factor Scalloped (Sd) regulates cell proliferation and organ growth in the Hippo pathway. The research identified and characterized LmSd, showing its importance in wing formation and development by controlling cell proliferation and inhibiting apoptosis. The study found that silencing of LmSd resulted in deformed wings and abnormal wing pad closure during molting.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Immunology
Yao-Wen Hu, Shao-Hua Wang, Ya Tang, Guo-Qiang Xie, Yan-Juan Ding, Qing-Ye Xu, Bin Tang, Long Zhang, Shi-Gui Wang
Summary: Locusta migratoria manilensis shows a trade-off between reproduction and immunity when resources are limited, leading to inhibited ovarian development and greatly decreased total fecundity when resisting pathogen invasion.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yiyan Zhao, Weimin Liu, Xiaoming Zhao, Zhitao Yu, Hongfang Guo, Yang Yang, Bernard Moussian, Kun Yan Zhu, Jianzhen Zhang
Summary: In this study, the researchers found that LmLPR is mainly expressed in the fat body, ovary, and integument of migratory locusts, and it is involved in lipid uptake and accumulation in the ovary. Silencing LmLPR significantly decreased the neutral lipid content in the ovary and led to impaired ovarian development.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
X-J Liu, X-Y Liang, J. Guo, X-K Shi, H. Merzendorfer, K. Y. Zhu, J-Z Zhang
Summary: The vacuolar-type H+-ATPase (V-ATPase) is an important gene for survival and midgut development in Locusta migratoria, as demonstrated by differential expression in various tissues and developmental stages, as well as the significant suppression of the gene leading to mortality in injected nymphs. This suggests that LmV-ATPase a could be a potential target for RNAi-based control against locusts.
INSECT MOLECULAR BIOLOGY
(2022)
Article
Entomology
Xuekai Shi, Shuai Li, Lin Yang, Xiaojian Liu, Hans Merzendorfer, Kun Yan Zhu, Jianzhen Zhang
Summary: In this study, a Chc ortholog was identified from a Locusta migratoria transcriptome database, and RNA interference experiments confirmed its important role in the development of L. migratoria, affecting growth, development, and reproduction.
Article
Entomology
Xiao-Jian Liu, Guo Jun, Xiao-Yu Liang, Xue-Yao Zhang, Ting-Ting Zhang, Wei-Min Liu, Jian-Zhen Zhang, Min Zhang
Summary: The study identified the LmE93 gene in Locusta migratoria, showing its crucial roles in metamorphosis, cuticle, wing, and ovarian development. Injection of dsLmE93 hindered the metamorphosis process and resulted in supernumerary nymphal stages in locusts.
Article
Biology
Qian Wang, Yusheng Liu, Xiangchu Yin
Summary: This study demonstrates that different food plants significantly affect the growth and development of L. migratoria manilensis, as well as the structure of gut bacterial communities. The gut microbiota plays an essential role in the adaptation of the locust host.
Article
Entomology
Xuekai Shi, Xiaojian Liu, Kristopher Silver, Kun Yan Zhu, Jianzhen Zhang
Summary: The study identified the important role of LmLgl in maintaining tissue structure and normal morphogenesis in Locusta migratoria. Silencing LmLgl resulted in increased body size but decreased bodyweight, as well as abnormal morphological structure in the midgut. This discovery may contribute to the development of dsRNA-based biological pesticides for insect pest management.