Journal
INSECT MOLECULAR BIOLOGY
Volume 19, Issue 3, Pages 399-408Publisher
WILEY
DOI: 10.1111/j.1365-2583.2010.01001.x
Keywords
JHEH; cDNA cloning; epoxide hydrolase; detoxification enzyme; Coleoptera
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Funding
- Program for the Promotion of Basic Research Activities for Innovative Biosciences (PROBRAIN)
- Ministry of Agriculture, Forestry, and Fisheries
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Juvenile hormone epoxide hydrolases (JHEHs) degrade juvenile hormones (JHs) and are important for JH titre regulation. Here, we report the cloning and analysis of five jheh-related (jheh-r1-r5) genes in the red flour beetle, Tribolium castaneum, a model species for the coleopteran insects. T. castaneum JHEH-r (TcJHEH-r) proteins show high homology to lepidopteran JHEHs and also to human microsomal epoxide hydrolase. In the phylogenetic tree, Tcjheh-rs were clustered, and interestingly, they were also clustered in the genome. Examination of enzymatic activities using recombinant TcJHEH-r proteins showed that TcJHEH-r3 had strong degradation activity for JH III, whereas TcJHEH-r4 had weak activity. The study has yielded significant information that will facilitate further analysis of JHEHs and epoxide hydrolases.
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