Article
Genetics & Heredity
Chao Tianle, Yang Liuxu, Lou Delong, Fan Yunhan, He Yu, Shan Xueqing, Xia Haitao, Wang Guizhi
Summary: This study analyzed the changes in miRNA expression profile in the brain tissue of honeybees after fluvalinate treatment. The differentially expressed miRNAs may be related to cell apoptosis and memory impairment.
FRONTIERS IN GENETICS
(2022)
Article
Agriculture, Dairy & Animal Science
Chao Tianle, Fan Yunhan, Lou Delong, Xia Haitao, Ma Lanting, Shan Xueqing, Yang Liuxu, He Yu, Wang Guizhi
Summary: This study performed transcriptome sequencing on bee brain tissues before and after fluvalinate treatment, detecting 546 differentially expressed genes involved in lipid metabolism, amino acid metabolism, visual transduction, and neural response. Protein-protein interaction network analysis identified five key genes that may play important roles in resistance to fluvalinate-induced honeybee brain nerve tissue damage. This study provides a detailed characterization and functional analysis of genes related to fluvalinate stimulation in honeybee brains.
ANIMAL BIOTECHNOLOGY
(2022)
Article
Agronomy
Qibao He, Qing Yang, Qiongqiong Liu, Zhaoyin Hu, Quan Gao, Yongcheng Dong, Jinjing Xiao, Linsheng Yu, Haiqun Cao
Summary: The study found that four commonly used chemical pesticides (beta-cypermethrin, chlorbenzuron, chlorothalonil, and pendimethalin) had effects on the growth of bee larvae, with beta-cypermethrin and chlorbenzuron having the most significant impact on the health of Apis mellifera ligustica and Apis cerana cerana. Additionally, a high dose of chlorothalonil also led to a decrease in adult bee numbers.
PEST MANAGEMENT SCIENCE
(2022)
Article
Genetics & Heredity
Shan Xueqing, Lou Delong, Wang Guizhi, Fan Yunhan, Yang Liuxu, Chao Tianle
Summary: This study discovered the effects of fluvalinate on the circular RNA expression profile of honeybee brain tissue, and identified five circular RNAs involved in apoptosis-related functions. It provides a useful reference for understanding the biological function of circular RNAs in honeybees.
FRONTIERS IN GENETICS
(2023)
Article
Developmental Biology
Xiyi Hu, Weixing Zhang, Xuepeng Chi, Hongfang Wang, Zhenguo Liu, Ying Wang, Lanting Ma, Baohua Xu
Summary: The mandibular gland of worker bees secretes fatty acids and pheromones, with significant changes in morphology and lipid composition observed during different developmental stages. Key lipids like phosphatidylcholine and triacylglycerol show dramatic alterations across stages, while differentially expressed genes are enriched in nutrient metabolic pathways, especially lipid metabolism, in 6-day and 15-day worker bees. This multi-omic study provides valuable insights into bee mandibular gland development and serves as a baseline for future research in this area.
DEVELOPMENTAL BIOLOGY
(2021)
Article
Entomology
Abdessamad Aglagane, Andrzej Oleksa, Omar Er-Rguibi, Adam Tofilski, El Hassan El Mouden, Ahmed Aamiri, Mohamed Aourir
Summary: This study assessed the conservation status of the Saharan honey bee in southeastern Morocco using genetic analysis. It found that the honey bees in Morocco have higher genetic diversity compared to European honey bees. However, hybridization with a different subspecies poses a threat to the genetic integrity of the Saharan honey bee. The study also discovered that human management intensity and precipitation are factors that affect the probability of a honey bee belonging to the Saharan subspecies.
Article
Biochemistry & Molecular Biology
Xiyi Hu, Ying Wang, Xuepeng Chi, Hongfang Wang, Zhenguo Liu, Lanting Ma, Baohua Xu
Summary: This study aimed to evaluate the impact of oleic acid (OA) supplementation on the synthesis of 10-hydroxy-2-decenoic acid (10-HDA) in Apis mellifera ligustica. The results showed that the addition of 8% OA had the greatest effect on promoting the synthesis of 10-HDA. Analysis of the molecular signature in the mandibular gland revealed significant remodeling of TAGs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Aleksandra Patenkovic, Marija Tanaskovic, Pavle Eric, Katarina Eric, Milica Mihajlovic, Ljubisa Stanisavljevic, Slobodan Davidovic
Summary: Urbanization impacts the genetic diversity of honey bees, causing high genetic differentiation between feral and managed colonies. Feral honey bees in urban areas have distinct patterns of genetic diversity and can adapt to human-modified environments.
SCIENTIFIC REPORTS
(2022)
Article
Ecology
Annik Imogen Gmel, Matthieu Guichard, Benjamin Dainat, Geoffrey Rhys Williams, Sonia Eynard, Alain Vignal, Bertrand Servin, Markus Neuditschko
Summary: This study analyzed the whole-genome queen genotypes of 265 Western honeybee colonies, revealing the genetic diversity and differences in genetic background among subspecies. The analysis of genetic frequencies and admixture levels provided insights into the population dynamics and identified genes associated with environmental adaptation and morphological phenotypes. Overall, this study provides fundamental information for conserving native honey bees.
ECOLOGY AND EVOLUTION
(2023)
Article
Veterinary Sciences
Nadezhda V. Ostroverkhova
Summary: Through studying the dark forest bee, it was found that microsatellite loci AC117, Ap243, and SV185 are associated with resistance to Nosema disease, providing potential markers for predicting the risk of developing Nosema infection in bee colonies.
VETERINARY SCIENCES
(2021)
Article
Multidisciplinary Sciences
M. Parejo, A. Talenti, M. Richardson, A. Vignal, M. Barnett, D. Wragg
Summary: This study utilized the unique characteristics of haploid honey bee drones and high-quality genetic data to establish the haplotype resource AmelHap. It demonstrated the utility of AmelHap in genotype imputation, association studies, population genetic analyses, and evolutionary analyses.
Article
Entomology
Eduard Musin, Kaspar Bienefeld, Hartmut Skerka, Jakob Wegener
Summary: Lack of efficient mating control is a limiting factor for honey bee selective breeding and conservation. This study presents two simplified, semi-automatized protocols for selective mating of queens and drones, and provides initial data on their effectiveness. The results demonstrate that effective mating control via delayed flight time mating is possible even in areas with a strong presence of other subspecies/breeds, but post-mating queen selection is necessary to avoid undesired crosses.
JOURNAL OF APICULTURAL RESEARCH
(2023)
Article
Biodiversity Conservation
Gerald Kwikiriza, Thapasya Vijayan, Papius Dias Tibihika, Manuel Curto, Gerold Winkler, Juliet Kigongo Nattabi, John Kariuki, Harald Meimberg
Summary: Despite challenges such as overfishing, climate change, and fish introductions, the study highlights the low levels of hybridization between different tilapiine species in Lake Victoria, Kenya. The research emphasizes the need for conservation measures to protect these fish populations.
CONSERVATION GENETICS
(2023)
Article
Biotechnology & Applied Microbiology
Jamal Momeni, Melanie Parejo, Rasmus O. Nielsen, Jorge Langa, Iratxe Montes, Laetitia Papoutsis, Leila Farajzadeh, Christian Bendixen, Eliza Cauia, Jean-Daniel Charriere, Mary F. Coffey, Cecilia Costa, Raffaele Dall'Olio, Pilar De la Rua, M. Maja Drazic, Janja Filipi, Thomas Galea, Miroljub Golubovski, Ales Gregorc, Karina Grigoryan, Fani Hatjina, Rustem Ilyasov, Evgeniya Ivanova, Irakli Janashia, Irfan Kandemir, Aikaterini Karatasou, Meral Kekecoglu, Nikola Kezic, Eniko Sz. Matray, David Mifsud, Rudolf Moosbeckhofer, Alexei G. Nikolenko, Alexandros Papachristoforou, Plamen Petrov, M. Alice Pinto, Aleksandr V. Poskryakov, Aglyam Y. Sharipov, Adrian Siceanu, M. Ihsan Soysal, Aleksandar Uzunov, Marion Zammit-Mangion, Rikke Vingborg, Maria Bouga, Per Kryger, Marina D. Meixner, Andone Estonba
Summary: The study utilized worker bee data from 22 populations in Europe to select informative SNPs for ancestry inference through two highly discriminative methods. This led to the development of an accurate predictive tool for determining the ancestry of European honey bees.
Article
Entomology
Ahmet Guler, Hasan Onder, Gokhan Kavak, Gokhan Toktay, Emre Ugurlutepe, Selim Biyik, Abdurrahman Aydin
Summary: This study investigated the effects of instrumental insemination and natural mating on selected and unselected traits in a breeding population. The results showed a significant difference between the two methods in terms of hygiene behavior, but no significant difference in performance phenotypes. It suggests that instrumental insemination can produce colonies with equivalent phenotypes compared to open-mated queens.
Editorial Material
Entomology
Beatrice R. Barletti, Ana Asensio, Carlo Polidori, Marino Quaranta, Pilar De la Rua
Summary: In this study, we rediscovered the presence of Lasioglossum marginatum in Eastern Sicily after 45 years without records, and its existence was confirmed by DNA barcoding. This species is the only known eusocial sweat bee with perennial colonies, suggesting its rarity in Sicily.
JOURNAL OF APICULTURAL RESEARCH
(2023)
Article
Entomology
Laura Bortolotti, Fabio Fiorillo, Raffaele Dall'Olio, Diego Cejas, Pilar de la Rua, Gherardo Bogo
Summary: This study compared different methods for detecting diploid males in bumble bees, with microsatellite analysis and wing geometric morphometrics showing the highest efficiency in distinguishing between diploid and haploid males. These methods were found to be the most valuable in terms of cost-effectiveness.
JOURNAL OF APICULTURAL RESEARCH
(2022)
Article
Entomology
Gherardo Bogo, Diego Cejas, Pilar De la Rua, Laura Bortolotti
Summary: The study used geometric morphometrics to investigate wing shape and size differences between diploid and haploid males of B. terrestris. Results showed that diploid male wings had significant shape changes, likely influenced by incubation temperature and food availability during larval development. Within colonies, wing geometric morphometrics may not accurately discriminate diploid males, but at the overall level (sample size of 500 individuals), it was precise in determining diploid males.
ENTOMOLOGIA EXPERIMENTALIS ET APPLICATA
(2022)
Article
Ecology
Carolina Bartolome, Maria Buendia-Abad, Concepcion Ornosa, Pilar De la Rua, Raquel Martin-Hernandez, Mariano Higes, Xulio Maside
Summary: Trypanosomatids, common parasites in bees, were studied for population diversity using genetic data from three single copy loci. The analysis showed that the parasites experienced purifying selection, similar diversity within species regardless of host, evidence of recombination among haplotypes, and no haplotype structuring by host. C. bombi had low synonymous variation, C. mellificae displayed extensive polymorphism, and L. passim showed intermediate values likely due to spread to new areas.
Article
Ecology
Vicente Martinez-Lopez, Carlos Ruiz, Irene Munoz, Concepcion Ornosa, Mariano Higes, Raquel Martin-Hernandez, Pilar De la Rua
Summary: Research shows the ability of N. ceranae to disperse across wild bee communities in semiarid Mediterranean ecosystems, with the prevalence of the pathogen not showing any phylogenetic signal. Moreover, N. apis was detected in an Eucera species, marking the second time it has been detected in a host other than the honey bee.
Article
Multidisciplinary Sciences
Niels Piot, Oliver Schweiger, Ivan Meeus, Orlando Yanez, Lars Straub, Laura Villamar-Bouza, Pilar De la Rua, Laura Jara, Carlos Ruiz, Martin Malmstrom, Sandra Mustafa, Anders Nielsen, Marika Mand, Reet Karise, Ivana Tlak-Gajger, Erkay Ozgor, Nevin Keskin, Virginie Dievart, Anne Dalmon, Anna Gajda, Peter Neumann, Guy Smagghe, Peter Graystock, Rita Radzeviciute, Robert J. Paxton, Joachim R. de Miranda
Summary: The study reveals that both biotic and abiotic factors, including managed host species, climatic variables, and beekeeping practices, influence the prevalence of viruses in wild bee populations.
SCIENTIFIC REPORTS
(2022)
Article
Entomology
David Lugo, Gustavo Pena, Pilar de la Rua, Carlos Ruiz
Summary: This study updates the knowledge about wild bees of the genus Hylaeus in the Canary Islands and identifies two new exotic species. The flight season and habitat of these exotic species show little overlap with those of the endemic species, suggesting potentially insignificant negative effects on the endemic species.
JOURNAL OF APICULTURAL RESEARCH
(2022)
Article
Entomology
Alvaro Uruena, Vicente Martinez-Lopez, William de Jesus May-Itza, Jose Javier Guadalupe Quezada-Euan, Pilar De la Rua
Summary: There are significant differences between different populations of N. perilampoides, showing a pattern of isolation by distance and possibly influenced by the Sierra Madre as a biogeographical barrier. The results suggest that colony exchange among regions should be restricted to preserve the diversity of this species.
JOURNAL OF APICULTURAL RESEARCH
(2022)
Article
Ecology
Vicente Martinez-Lopez, Carlos Ruiz, Pilar de la Rua
Summary: The increasing demand for food due to the growing human population has led to an increase in plant production. Many crops rely on insect pollination, with honey bees being traditionally viewed as the main provider of crop pollination services. However, the seasonal movement of beehives for pollination purposes may have negative impacts on bee health by spreading pathogens and parasites.
INTERNATIONAL JOURNAL FOR PARASITOLOGY-PARASITES AND WILDLIFE
(2022)
Article
Green & Sustainable Science & Technology
Simon Hodge, Oliver Schweiger, Alexandra-Maria Klein, Simon G. Potts, Cecilia Costa, Matthias Albrecht, Joachim R. de Miranda, Marika Mand, Pilar De la Rua, Maj Rundlof, Eleanor Attridge, Robin Dean, Philippe Bulet, Denis Michez, Robert J. Paxton, Aurelie Babin, Nicolas Cougoule, Marion Laurent, Anne-Claire Martel, Laurianne Paris, Marie-Pierre Riviere, Eric Dubois, Marie-Pierre Chauzat, Karim Arafah, Dalel Askri, Sebastien N. Voisin, Tomasz Kiljanek, Irene Bottero, Christophe Dominik, Giovanni Tamburini, Maria Helena Pereira-Peixoto, Dimitry Wintermantel, Tom D. Breeze, Elena Cini, Deepa Senapathi, Gennaro Di Prisco, Piotr Medrzycki, Steffen Hagenbucher, Anina Knauer, Janine M. Schwarz, Risto Raimets, Vicente Martinez-Lopez, Kjell Ivarsson, Chris Hartfield, Pamela Hunter, Mark J. E. Brown, Jane C. Stout
Summary: To understand the negative impacts on insects in agricultural landscapes, it is important to assess multiple stressors and their interactions across large geographical scales. The PoshBee project established a network of 128 study sites across Europe and collected measurements and samples related to various aspects of the bees' environment. This paper describes the development process, rationale, and outcomes of the project, highlighting the challenges and actions that may benefit future large-scale studies.
Article
Multidisciplinary Sciences
Nonno Hasegawa, Maeva A. Techer, Noureddine Adjlane, Muntasser Sabah al-Hissnawi, Karina Antunez, Alexis Beaurepaire, Krisztina Christmon, Helene Delatte, Usman H. Dukku, Nurit Eliash, Mogbel A. A. El-Niweiri, Olivier Esnault, Jay D. Evans, Nizar J. Haddad, Barbara Locke, Irene Munoz, Gregoire Noel, Delphine Panziera, John M. K. Roberts, Pilar De la Rua, Mohamed A. Shebl, Zoran Stanimirovic, David A. Rasmussen, Alexander S. Mikheyev
Summary: This study used phylogeographic analysis based on whole-genome data to reconstruct the origins and spread of deformed wing viruses (DWV) transmitted by the varroa mite. The results suggest that DWV-A likely originated in East Asia and spread in the mid-20th century, while DWV-B was acquired more recently from a source outside East Asia and appears to be absent from the original varroa host.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Environmental Sciences
Alvaro Uruena, Nuria Blasco-Lavilla, Pilar De la Rua
Summary: Honey bees' health is affected by various factors, including agrochemical use and diseases. This study examines the interaction between pesticides and Nosema ceranae, a microsporidium, and their effects on gene expression, sugar consumption, and mortality in honey bees. The results show that exposure to sulfoxaflor increases sugar intake and alters gene expression in bees infected with N. ceranae. Additionally, sulfoxaflor is the only pesticide that increased honey bee mortality. These findings highlight the importance of studying the interaction between stressors to understand their impact on honey bee and wild bee health.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Entomology
Antonio Jose Garcia-Meseguer, Adrian Villastrigo, Juana Maria Miron-Gaton, Andres Millan, Josefa Velasco, Irene Munoz
Summary: In this study, microsatellite markers were designed for the first time for evolutionary and ecological research on aquatic beetles from the genus Ochthebius. Next-generation sequencing technology was used to construct libraries and obtain 20 markers. The complete mitochondrial genomes of three Ochthebius species were also retrieved. The SSR panels and mitochondrial genomes will be valuable for future research on species identification, diversity, genetic structure, and population connectivity in highly dynamic and threatened habitats.