Journal
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
Volume 43, Issue 8, Pages 664-674Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.ibmb.2013.04.004
Keywords
Stress granules; BmN4 cells; Silkworm; Argonaute; Tudor-sn
Categories
Funding
- KAKENHI from the Japan Society for the Promotion of Science [22248004, 23580077]
- Grants-in-Aid for Scientific Research [23580077] Funding Source: KAKEN
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The Tudor-sn protein, which contains four staphylococcal nuclease domains and a Tudor domain, is a ubiquitous protein found in almost all organisms. It has been reported that Tudor-sn in mammals participates in various cellular pathways involved in gene regulation, cell growth, and development. In insects, we have previously identified a Tudor-sn ortholog in the silkworm, Bombyx mori, and detected its interactions between with Argonaute proteins. The role of Tudor-sn in silkworm, however, still remains largely unknown. In this study, we demonstrated that silkworm Tudor-sn is a stress granule (SG) protein, and determined its interactions with other SG proteins using Bimolecular Fluorescence Complementation assay and Insect Two-Hybrid method. Depletions of Argonaute proteins and SG-marker protein Tia1 by RNAi impaired the involvement of Tudor-sn in the SG formation. Protein domain deletion analysis of Tudor-sn demonstrated that SN2 is the key domain required for the aggregation of Tudor-sn in SGs. (C) 2013 Elsevier Ltd. All rights reserved.
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