Journal
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
Volume 40, Issue 1, Pages 49-57Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.ibmb.2009.12.009
Keywords
Haemaphysalis longicornis tick; GATA transcription factor; S6 kinase; RNA interference; Vitellogenin
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Funding
- Japan Society for the Promotion of Science (JSPS)
- Bio-orientated Technology Research Advancement Institution (BRAIN)
- Ministry of Education, Sports, Science, and Technology of Japan
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Blood feeding tightly regulates the reproductive cycles of ticks. Vitellogenesis and nutritional signaling are key events in the tick reproductive cycle. Here we report the identification of a GATA factor that is synthesized after a blood meal and acts as a transcriptional activator of vitellogenin (Vg), and the identification of an S6 kinase that is a transcription regulator of the amino acid signaling pathway. Tick GATA mRNA accumulated in the midgut prior to blood feeding. However, translation of GATA was activated by blood feeding because the GATA protein dramatically increased in the fat body of engorged females. RNA interference-mediated knockdown of S6 kinase and GATA factor revealed the involvements of S6 kinase in GATA activation and resulted in a significant inhibition of the major yolk protein vitellogenin in engorged ticks and effectively disrupting egg development after a blood meal. These results indicate that the GATA factor, a specific transcriptional activator of Vg gene, represents an important molecule for the regulation of tick vitellogenesis and reproduction. (C) 2010 Elsevier Ltd. All rights reserved.
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