Journal
INFLAMMATORY BOWEL DISEASES
Volume 14, Issue 4, Pages 480-490Publisher
OXFORD UNIV PRESS INC
DOI: 10.1002/ibd.20347
Keywords
serologic markers/testing; biomarkers; animal models; of lBD; inflammation in IBD
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Funding
- NATIONAL CENTER FOR RESEARCH RESOURCES [T32RR007019] Funding Source: NIH RePORTER
- NCRR NIH HHS [5 T32 RR07019] Funding Source: Medline
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Background: The diagnosis and classification of inflammatory bowel disease (IBD) require both clinical and histopathologic data. Serum biomarkers would be of considerable benefit to noninvasively monitor the progression of disease, assess effectiveness of therapies, and assist in understanding disease pathogenesis. Currently, there are limited noninvasive biomarkers for monitoring disease progression in animal IBD models, which are used extensively to develop new therapies and to understand IBD pathogenesis. Methods: Serum biomarkers of early and late IBD were identified using multianalyte profiling in nidrla(-/-) mice with IBD triggered by infection with Helicobacter bilis. The correlation of changes in these biomarkers with histopathology scores and clinical signs in the presence and in the absence of antibiotic treatment was determined. Results: Serum levels of interleukin (IL)-11, IL-17, 10-kDa interferon-gamma-inducible protein (IP-10), lymphotactin, monocyte chemoattractant protein (MCP)-1, and vascular cell adhesion molecule (VCAM)-1 were elevated early in IBD. In late, more severe IBD, serum levels of IL-11. IP-10, haptoglobin, matrix metalloproteinase-9, macrophage inflammatory protein (MIP)-1 gamma, fibrinogen, immunoglobulin A. MIP-3 beta (beta), VCAM-I, apolipoprotein (Apo) A L and IL-18 were elevated. All late serum biomarkers except Apo Al correlated with histopathology scores. Antibiotic treatment improved clinical signs of IBD and decreased mean serum values of many of the biomarkers. For all biomarkers, the individual pathology scores correlated significantly with individual serum analyte levels after treatment. Conclusions: Serum analyte measurement is a useful, noninvasive method for monitoring disease in a mouse model of bacterialinduced IBD.
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