Journal
INFLAMMATION RESEARCH
Volume 63, Issue 7, Pages 557-568Publisher
SPRINGER BASEL AG
DOI: 10.1007/s00011-014-0725-5
Keywords
Oral keratinocytes; Alpha 7 nicotinic receptor; Acetylcholine; Periodontal disease; Inflammation
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Funding
- Dunhill Medical Trust
- The Dunhill Medical Trust [R203/0511] Funding Source: researchfish
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The alpha 7 nicotinic receptor (alpha 7nAChR) is expressed by oral keratinocytes. alpha 7nAChR activation mediates anti-inflammatory responses. The objective of this study was to determine if alpha 7nAChR activation inhibited pathogen-induced interleukin-8 (IL-8) expression by oral keratinocytes. Periodontal tissue expression of alpha 7nAChR was determined by real-time PCR. OKF6/TERT-2 oral keratinocytes were exposed to Porphyromonas gingivalis in the presence and absence of a alpha 7nAChR agonist (PHA-543613 hydrochloride) alone or after pre-exposure to a specific alpha 7nAChR antagonist (alpha-bungarotoxin). Interleukin-8 (IL-8) expression was measured by ELISA and real-time PCR. Phosphorylation of the NF-kappa B p65 subunit was determined using an NF-kappa B p65 profiler assay and STAT-3 activation by STAT-3 in-cell ELISA. The release of ACh from oral keratinocytes in response to P. gingivalis lipopolysaccharide was determined using a GeneBLAzer M3 CHO-K1-bla cell reporter assay. Expression of alpha 7nAChR mRNA was elevated in diseased periodontal tissue. PHA-543613 hydrochloride inhibited P. gingivalis-induced expression of IL-8 at the transcriptional level. This effect was abolished when cells were pre-exposed to a specific alpha 7nAChR antagonist, alpha-bungarotoxin. PHA-543613 hydrochloride downregulated NF-kappa B signalling through reduced phosphorylation of the NF-kappa B p65-subunit. In addition, PHA-543613 hydrochloride promoted STAT-3 signalling by maintenance of phosphorylation. Furthermore, oral keratinocytes upregulated ACh release in response to P. gingivalis lipopolysaccharide. These data suggest that alpha 7nAChR plays a role in regulating the innate immune responses of oral keratinocytes.
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