Article
Immunology
Mercedes Lopez-Santalla, Carmen Conde, Angela Rodriguez-Trillo, Marina Garin
Summary: Rheumatoid arthritis is an autoimmune disease with unresolved treatment issues. Mesenchymal stem cell therapy has emerged as a potential alternative, but its effectiveness may vary in different arthritis models.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Sebastien Fauteux-Daniel, Laura Merlo M. Pich, Charlotte Girard-Guyonvarc'h, Assunta Caruso, Emiliana Rodriguez, Cem Gabay
Summary: This study investigates the expression and function of IL-18 and IL-18BP in K/BxN serum transfer arthritis (STA) model. The results show that IL-18 and IL-18BP mRNA levels are increased in arthritic joints, but the IL-18/IL-18BP balance is not involved in the regulation of STA.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Kathannan Sankar, Hyung Joo Yoon, Young Bo Lee, Kyeong Yong Lee
Summary: Bumble bees are important alternative pollinators and model insects. This study identified stable reference genes for the bumble bee species and their different lines and tissues using RT-qPCR procedures and four analysis programs. The results emphasized the need to evaluate the stability of candidate reference genes for any differently designed lines and tissue conditions in bumble bee species.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Zhiwei Chen, Nigel G. Halford, Chenghong Liu
Summary: Real-time quantitative PCR is a technique for measuring the content of a specific nucleic acid sequence in a sample, and it can be divided into absolute and relative quantitative methods. Relative quantification is mainly used for gene expression in functional genomics and transcriptome studies. However, to accurately use this technology, key points such as designing specific primers, selecting appropriate reference genes, and performing scientific calculations and statistics are crucial. This article proposes a workflow for relative quantitative PCR and introduces relevant points to help beginners better understand and utilize this technology.
Article
Chemistry, Multidisciplinary
Jing Tu, Yi Qiao, Yuhan Luo, Naiyun Long, Zuhong Lu
Summary: DNA quantification is crucial in life sciences research, and real-time MDA technology can provide template amount information during whole-genome amplification with a very low detection limit, offering a promising tool to quantify entire DNA without sacrificing sample completeness or introducing redundant steps.
Article
Chemistry, Analytical
Liang Gong, Siyue Yao, Yidong He, Chengliang Liu
Summary: A new qPCR data analysis method called AERKM is proposed to achieve more accurate results by introducing amplification efficiency (AE) for error correction. It has been verified to have higher precision and stability in 5-point 10-fold gradient qPCR tests of 63 genes.
ANALYTICAL CHEMISTRY
(2023)
Article
Immunology
Thomas Weissmann, Michael Rueckert, Jian-Guo Zhou, Michaela Seeling, Sebastian Lettmaier, Anna-Jasmina Donaubauer, Falk Nimmerjahn, Oliver J. Ott, Markus Hecht, Florian Putz, Rainer Fietkau, Benjamin Frey, Udo S. Gaipl, Lisa Deloch
Summary: Osteoarthritis is a common degenerative joint disease, particularly affecting the ankle and foot. Low-dose radiotherapy has been shown to be highly effective in treating osteoarthritis, with analgesic effects mediated by an interplay of cellular and immune factors, especially in younger patients.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Horticulture
Rundong Yao, Xiaolou Huang, Hanqing Cong, Fei Qiao, Yunjiang Cheng, Yeyuan Chen
Summary: Real-Time PCR is a rapid and sensitive technique widely used to determine gene expression. This study identified 10 candidate reference genes in mangos and evaluated their stability using professional software. TUBB gene was found to be the most stable and suitable reference gene.
Article
Pharmacology & Pharmacy
Jing Tang, Xiaolan Cheng, Shiyu Yi, Yuanyuan Zhang, Zhigang Tang, Yutong Zhong, Qiuping Zhang, Bin Pan, Yubin Luo
Summary: This study investigated the therapeutic effect of the diterpenoid EFL2 derived from Euphorbia seeds on rheumatoid arthritis and explored its potential anti-arthritic mechanisms. The results demonstrated that EFL2 effectively reduced inflammation and interfered with the TLR7-mediated signaling pathway, providing a potential treatment option for rheumatoid arthritis.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Plant Sciences
Rohit Bharati, Madhab Kumar Sen, Ram Kumar, Aayushi Gupta, Jana Ziarovska, Eloy Fernandez-Cusimamani, Olga Leuner
Summary: This study assessed the suitability of ten commonly used reference genes as optimal reference genes in M. officinalis under different abiotic stress conditions and plant organs. The combination of EF-1 alpha and GAPDH was recommended as the best reference genes for gene expression studies in M. officinalis.
Article
Rheumatology
Desiree Luis-Rodriguez, Javier Donate-Correa, Ernesto Martin-Nunez, Carla Ferri, Victor G. Tagua, Atteneri Perez Castro, Carmen Mora-Fernandez, Juan F. Navarro-Gonzalez
Summary: This study found that serum uric acid levels are associated with inflammation in asymptomatic hyperuricaemia (AHU) patients, and UA levels may be an inducer of subclinical inflammation. Therapeutic reduction of UA levels was associated with modulation of inflammatory characteristics.
Article
Multidisciplinary Sciences
Joshua T. Atkinson, Lin Su, Xu Zhang, George N. Bennett, Jonathan J. Silberg, Caroline M. Ajo-Franklin
Summary: In this study, biosensors that produce electrical readouts with fast detection times were developed by combining synthetic biology and materials engineering. Escherichia coli were programmed to produce an electrical current in response to specific chemicals, allowing for rapid detection of thiosulfate and an endocrine disruptor. This new platform provides design rules for sensing various chemicals and offers a potential solution for safeguarding ecological and human health.
Article
Plant Sciences
Fangzhou Zhao, Nathan A. Maren, Pawel Z. Kosentka, Ying-Yu Liao, Hongyan Lu, James R. Duduit, Debao Huang, Hamid Ashrafi, Tuanjie Zhao, Alejandra Huerta, Thomas G. Ranney, Wusheng Liu
Summary: Optimizing primer design and qPCR parameters are crucial for improving primer efficiency and data analysis accuracy.
HORTICULTURE RESEARCH
(2021)
Article
Agronomy
Shengyi Bai, Xiaomin Wang, Meng Guo, Guoxin Cheng, Abid Khan, Wenkong Yao, Yanming Gao, Jianshe Li
Summary: This study screened out the most stable reference genes and studied the expression of related genes in the interaction between tomatoes and powdery mildew, providing a calibration basis for the expression of related functional genes.
Article
Immunology
Xiaolin Jiang, Yong Jiang, Feng Ye
Summary: This study used real-time quantitative PCR to detect Mucorales and Aspergillus in formalin-fixed, paraffin-embedded samples. The combination of Mucorales RQ-PCR and Aspergillus RQ-PCR was recommended as a screening tool for detecting suspected mucormycosis and/or aspergillosis samples.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Immunology
Trinidad Montero-Melendez, Thomas Gobbetti, Sadani N. Cooray, Thomas E. N. Jonassen, Mauro Perretti
JOURNAL OF IMMUNOLOGY
(2015)
Review
Immunology
Trinidad Montero-Melendez
SEMINARS IN IMMUNOLOGY
(2015)
Review
Pharmacology & Pharmacy
Mauro Perretti, Xavier Leroy, Elliot J. Bland, Trinidad Montero-Melendez
TRENDS IN PHARMACOLOGICAL SCIENCES
(2015)
Article
Biochemistry & Molecular Biology
Robert Haas, Joanne Smith, Vidalba Rocher-Ros, Suchita Nadkarni, Trinidad Montero-Melendez, Fulvio D'Acquisto, Elliot J. Bland, Michele Bombardieri, Costantino Pitzalis, Mauro Perretti, Federica M. Marelli-Berg, Claudio Mauro
Article
Pharmacology & Pharmacy
Raffaele Simeoli, Giuseppina Mattace Raso, Claudio Pirozzi, Adriano Lama, Anna Santoro, Roberto Russo, Trinidad Montero-Melendez, Roberto Berni Canani, Antonio Calignano, Mauro Perretti, Rosaria Meli
BRITISH JOURNAL OF PHARMACOLOGY
(2017)
Article
Biochemistry & Molecular Biology
Trinidad Montero-Melendez, Rachel A. E. Forfar, Jennifer M. Cook, Jeffrey C. Jerman, Debra L. Taylor, Mauro Perretti
CELLULAR AND MOLECULAR LIFE SCIENCES
(2017)
Article
Biochemistry & Molecular Biology
Mila F. M. Madeira, Celso M. Queiroz-Junior, Trinidad Montero-Melendez, Silvia M. C. Werneck, Joice D. Correa, Frederico M. Soriani, Gustavo P. Garlet, Daniele G. Souza, Mauro M. Teixeira, Tarcilia A. Silva, Mauro Perretti
Article
Immunology
Magdalena K. Kaneva, Karin V. Greco, Sarah E. Headland, Trinidad Montero-Melendez, Prashant Mori, Kevin Greenslade, Costantino Pitzalis, Adrian Moore, Mauro Perretti
JOURNAL OF IMMUNOLOGY
(2017)
Article
Pathology
Trinidad Montero-Melendez, Mita F. M. Madeira, Lucy V. Norling, Asil Alsam, Michael A. Curtis, Tarcilia A. da Silva, Mauro Perretti
AMERICAN JOURNAL OF PATHOLOGY
(2014)
Article
Medicine, Research & Experimental
Jesmond Dalli, Lucy V. Norling, Trinidad Montero-Melendez, Donata Federici Canova, Hazem Lashin, Anton M. Pavlov, Gleb B. Sukhorukov, Charles J. Hinds, Mauro Perretti
EMBO MOLECULAR MEDICINE
(2014)
Article
Multidisciplinary Sciences
Trinidad Montero-Melendez, Ai Nagano, Claude Chelala, Andrew Filer, Christopher D. Buckley, Mauro Perretti
NATURE COMMUNICATIONS
(2020)
Review
Biochemistry & Molecular Biology
Camilla S. A. Davan-Wetton, Emanuela Pessolano, Mauro Perretti, Trinidad Montero-Melendez
Summary: Cellular senescence, defined as an irreversible cell cycle arrest with increased cellular growth and metabolic activity, has become a focus of biomedical research. The dual nature of senescence, beneficial/detrimental, is linked to contextual aspects, with the pro-resolving model suggesting that pathology arises from incomplete senescence program. Therapeutic approaches based on senescence include induction, prevention, and clearance of senescent cells, as well as the use of senolytic and senomorphic drugs. Challenges and gaps in research need to be addressed to promote the clinical success of senescence-based therapies.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Cell Biology
Silvia Oggero, Monica de Gaetano, Simone Marcone, Stephen Fitzsimons, Andreia L. Pinto, Dinara Ikramova, Mary Barry, David Burke, Trinidad Montero-Melendez, Dianne Cooper, Thomas Burgoyne, Orina Belton, Lucy V. Norling, Eoin P. Brennan, Catherine Godson, Mauro Perretti
Summary: Extracellular vesicles released by mixed aggregates of monocytes and platelets have pro-inflammatory effects on endothelial cells and atherosclerotic plaques, and modulating platelet activation can alter the composition and actions of these vesicles, potentially providing therapeutic avenues for atherosclerosis treatment. Patients with coronary artery disease requiring intervention show elevated levels of specific EV subsets in plasma.
JOURNAL OF EXTRACELLULAR VESICLES
(2021)
Article
Biochemistry & Molecular Biology
Raquel M. Marques, Maria Gonzalez-Nunez, Mary E. Walker, Esteban A. Gomez, Romain A. Colas, Trinidad Montero-Melendez, Mauro Perretti, Jesmond Dalli
Summary: Regulatory T-cells play a central role in maintaining homeostasis and resolving inflammation. ALOX15 was found to regulate key aspects of T-reg biology, with its inhibition or deletion leading to altered gene expression and metabolism in T-regs. Incubation with ALOX15-derived pro-resolving mediators rescued the expression of key regulatory genes in T-regs with inhibited ALOX15 activity.
CELL DEATH AND DIFFERENTIATION
(2021)