Journal
INFLAMMATION
Volume 37, Issue 6, Pages 1925-1931Publisher
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s10753-014-9925-y
Keywords
selenium deficiency; lipopolysaccharide (LPS); mastitis; cytokine; MPO
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Funding
- China Postdoctoral Science Foundation [2013M540255]
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Selenium (Se) is an essential micronutrient that plays a critical role in anti-inflammatory processes and antioxidant defense system. In this study, we investigated the effects of dietary selenium deficiency on lipopolysaccharide (LPS)-induced mastitis in mouse models. Se content in the liver was assessed by fluorescent atomic absorption spectrometry. Glutathione peroxidase (GPx) activity in the blood, myeloperoxidase (MPO) activity, tumor necrosis actor alpha (TNF-alpha), and interleukin (IL)-1 beta in the supernatant of the mammary tissue were determined according to the corresponding kits. Cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) expressions were evaluated by Western blotting. The results showed that the Se-deficient mouse model was successfully replicated, and selenium deficiency exacerbated mammary gland histopathology, increased the expressions of TNF-alpha and IL-1 beta, and facilitated the activation of iNOS and COX-2 in LPS-induced mouse mastitis. In conclusion, our studies demonstrated that selenium deficiency resulted in more severe inflammatory response in LPS-induced mouse mastitis.
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