Article
Microbiology
Christophe Le Terrier, Clement Viguier, Patrice Nordmann, Alejandro J. Vila, Laurent Poirel
Summary: Taniborbactam showed excellent activity against B1 MBLs, except for SIM-1. VIM-1-like enzymes, particularly VIM-83, were less inhibited by Taniborbactam compared to VIM-2-like.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Microbiology
Magdalena Zalacain, Clarisse Lozano, Agustina Llanos, Nicolas Sprynski, Thomas Valmont, Cyntia De Piano, David Davies, Simon Leiris, Carole Sable, Adeline Ledoux, Ian Morrissey, Marc Lemonnier, Martin Everett
Summary: The global dissemination of metallo-beta-lactamase (MBL)-producing carbapenem-resistant Enterobacterales (CRE) is a serious public health concern, with NDM (New Delhi MBL) being a major cause of carbapenem therapy failures and limited treatment options due to additional resistance determinants carried on promiscuous plasmids encoding the NDM gene. ANT2681, a specific MBL inhibitor, shows potent activity against NDM enzymes and offers a potential new therapeutic option to treat serious infections caused by NDM-CRE.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Multidisciplinary Sciences
M. Wilamowski, D. A. Sherrell, Y. Kim, A. Lavens, R. W. Henning, K. Lazarski, A. Shigemoto, M. Endres, N. Maltseva, G. Babnigg, S. C. Burdette, V. Srajer, A. Joachimiak
Summary: Serial x-ray crystallography can reveal binding events and chemical conversions during enzymatic reactions. This study used time-resolved serial synchrotron crystallography to analyze the structure, binding, and cleavage of moxalactam antibiotic in L1 metallo-beta-lactamase from Stenotrophomonas maltophilia.
NATURE COMMUNICATIONS
(2022)
Article
Infectious Diseases
P. Urbanowicz, R. Izdebski, A. Baraniak, D. Zabicka, W. Hryniewicz, M. Gniadkowski
Summary: This study identified the molecular and genomic background of the rapid increase of MPPA in Poland, with multi-clonal spread as the leading factor. Various novel and specific characteristics of MPPA were also revealed through the analysis.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2021)
Article
Infectious Diseases
Areti Tychala, Georgios Meletis, Paraskevi Mantzana, Angeliki Kassomenaki, Charikleia Katsanou, Aikaterini Daviti, Lydia Kouroudi, Lemonia Skoura, Efthymia Protonotariou
Summary: The prompt detection of carbapenemases among Gram-negative bacteria is vital for infection control. This study evaluated a new immunochromatographic assay for detecting carbapenemases and found it to be highly effective and faster than traditional methods. The assay detected more carbapenemases than the standard test, especially in certain types of bacteria.
Article
Chemistry, Applied
Andrei Baran, Jevgenijs Kuzmins, Jevgenijs Kuznecovs, Alistair J. M. Farley, Tharindi Panduwawala, Anete Parkova, Pavel A. Donets, Jurgen Brem, Edgars Suna, Christopher J. Schofield, Kirill Shubin
Summary: We developed a new synthetic route for the preparation of metallo-beta-lactamase inhibitor pre-candidate EBL-3183 on a kilogram scale. The process involves starting from commercially available indole-2-carboxylate and using an Ellman auxiliary approach coupled with ruthenium-catalyzed stereoselective reduction to introduce chirality. The key spirocyclic cyclobutane motif was assembled using a diastereomerically pure epoxide building block. The prepared EBL-3183 was of sufficient quantity and quality for preclinical studies.
ORGANIC PROCESS RESEARCH & DEVELOPMENT
(2023)
Article
Multidisciplinary Sciences
Azhar Salari-jazi, Karim Mahnam, Parisa Sadeghi, Mohamad Sadegh Damavandi, Jamshid Faghri
Summary: The study conducted multi-step virtual screening in the NPASS database to identify potential metallo-beta -lactamase inhibitors. The efficacy of these compounds was evaluated through molecular docking and molecular dynamics simulations, while protein sequence alignment and pharmacophore modeling revealed conserved residues.
SCIENTIFIC REPORTS
(2021)
Article
Infectious Diseases
Nouf Al-Rashed, Khalid M. Bindayna, Mohammad Shahid, Nermin Kamal Saeed, Abdullah Darwish, Ronni Mol Joji, Ali Al-Mahmeed
Summary: This study collected 50 carbapenem-resistant Acinetobacter baumannii (CRAB) isolates from four main hospitals in Bahrain. The results showed that all isolates were resistant to imipenem, meropenem, and cephalosporins, and harbored various resistance genes. The study emphasizes the importance of strengthening surveillance and stringent infection control measures in clinical settings to prevent the further spread of such isolates.
Article
Chemistry, Multidisciplinary
Katarzyna Palica, Manuela Voracova, Susann Skagseth, Anna Andersson Rasmussen, Lisa Allander, Madlen Hubert, Linus Sandegren, Hanna-Kirstirep Schroder Leiros, Hanna Andersson, Mate Erdelyi
Summary: Infectious diseases remain a major concern for public health in the 21st century, and the development of antimicrobial resistance by microbes is a significant problem. The study found that phosphonamidate compounds have the potential to become a new generation of antibiotic therapeutics to combat metallo-beta-lactamase-resistant bacteria.
Article
Microbiology
Xiaohui Li, Qian Wang, Ji Zheng, Yan Guan, Chennan Liu, Jiangxue Han, Sihan Liu, Tianjun Liu, Chunling Xiao, Xiao Wang, Yishuang Liu
Summary: PHT427 was identified as a novel NDM-1 inhibitor that restored the susceptibility of meropenem against NDM-1 resistant bacteria. It showed inhibitory activity against NDM-1 by targeting the zinc ions at the active site and catalytic key amino acid residues. This study suggests that PHT427 could be a promising lead compound for the development of drugs against carbapenem-resistant bacteria.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Chemistry, Medicinal
Yan Guo, Yanan Yang, Xiangzhu Xu, Li Li, Yonglin Zhou, Guobin Jia, Lijuan Wei, Qinlei Yu, Jianfeng Wang
Summary: This study identifies fisetin as an effective inhibitor of NDM-1, reducing bacterial resistance to antibiotics. Combining fisetin with meropenem significantly attenuates resistance and shows synergistic effects in vitro and in vivo. Molecular simulations reveal the mechanism of fisetin's inhibitory activity on NDM-1. Fisetin represents a promising strategy for combating carbapenem-resistant bacterial infections.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Infectious Diseases
Michelle Lee, Taylor Abbey, Mark Biagi, Eric Wenzler
Summary: The combination of aztreonam and ceftazidime-avibactam shows promising results against SBL and MBL-producing Enterobacterales, although data on Pseudomonas aeruginosa are lacking. Subinhibitory concentrations of the two drugs produced synergy and restored bactericidal activity in most isolates tested, suggesting it may be a viable treatment option.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2021)
Article
Biochemistry & Molecular Biology
Rong Li, Huilin Su, Wei Chen, Yu-Hang Yan, Cong Zhou, Luohe Mou, Huan Yang, Shan Qian, Zhouyu Wang, Lingling Yang, Guo-Bo Li
Summary: As an important mechanism of ss-lactam antimicrobial resistance, metallo-ss-lactamases (MBLs) have gained increasing attention worldwide. However, there is still a lack of clinically useful drugs to combat MBL-mediated resistance. In this study, 1H-imidazole-2-carboxylic acid was identified as a core metal-binding pharmacophore (MBP) to target multiple B1 MBLs, and further structural optimization led to the discovery of more potent MBL inhibitors.
BIOORGANIC & MEDICINAL CHEMISTRY
(2022)
Article
Chemistry, Medicinal
Yuwen Jia, Barbara Schroeder, Yvonne Pfeifer, Christopher Froehlich, Lihua Deng, Christoph Arkona, Benno Kuropka, Jana Sticht, Kenichi Ataka, Silke Bergemann, Gerhard Wolber, Christoph Nitsche, Martin Mielke, Hanna-Kirsti S. Leiros, Guido Werner, Joerg Rademann
Summary: Carbapenem resistance caused by metallo-β-lactamases (MBLs) such as NDM-1 is a significant threat to the effectiveness of β-lactam antibiotics. In this study, 8-hydroxy- and 8-sulfonamido-quinoline-2-carboxylic acids were developed as potent inhibitors of NDM-1 with improved activity and binding affinity. These inhibitors formed reversible protein-inhibitor complexes with two zinc ions and showed remarkable entropic gain in NDM-1 structure. They effectively reduced the minimum inhibitory concentrations (MIC) of meropenem for multidrug-resistant bacteria expressing bla (NDM-1) and clinical strains without toxicity to human cells.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Radhika Mehta, Dann D. Rivera, David J. Reilley, Dominique Tan, Pei W. Thomas, Abigail Hinojosa, Alesha C. Stewart, Zishuo Cheng, Caitlyn A. Thomas, Michael W. Crowder, Anastassia N. Alexandrova, Walter Fast, Emily L. Que
Summary: The development of a fluorescent probe that reports on the metalation state of NDM within Escherichia coli provides a novel tool to study metal ion sequestration in host-pathogen interactions. The probe selectively coordinates the dizinc metal cluster of NDM and offers a way to monitor the enzyme's response to metal ions.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Biology
Arijit Pal, Anusri Tripathi
COMPUTATIONAL BIOLOGY AND CHEMISTRY
(2016)
Article
Biotechnology & Applied Microbiology
Lena Dhara, Anusri Tripathi, Arijit Pal
BIOMED RESEARCH INTERNATIONAL
(2013)
Article
Biotechnology & Applied Microbiology
A. Pal, A. Tripathi
JOURNAL OF APPLIED MICROBIOLOGY
(2019)
Article
Immunology
Arijit Pal, Anusri Tripathi
Article
Public, Environmental & Occupational Health
Arijit Pal, Anusri Tripathi
JOURNAL OF INFECTION AND PUBLIC HEALTH
(2020)
Article
Immunology
Arijit Pal, Anusri Tripathi
MICROBIAL PATHOGENESIS
(2020)
Article
Biotechnology & Applied Microbiology
Arijit Pal, Indrani Bhattacharyya, Anusri Tripathi
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2020)
Article
Chemistry, Physical
Deepak Patil, Sivanandam Aravindan, Arijit Pal, Wajihul H. Khan, Pragya Pragya, Swati Pundir, Immaculata Xess, Sarita Mohapatra, Vivekanandan Perumal, P. Venkateswara Rao
Summary: This study developed a simple method for making superhydrophobic micro-structured antibacterial polymer surfaces for coating-free catheters. The results showed that the pillar-structured surface exhibited higher water repellency and more efficiently reduced bacterial attachment compared to a plain surface.
SURFACES AND INTERFACES
(2023)
Article
Immunology
Arijit Pal, Lena Dhara, Anusri Tripathi
INDIAN JOURNAL OF MEDICAL RESEARCH
(2019)