Article
Food Science & Technology
Jihong Li, Eric Mi, Arhat Pradhan, Bruce A. McClane
Summary: This study investigated the influence of NanR on c-cpe strains. In sporulation medium, the nanR null mutants showed lower sialidase activity, sporulation, and CPE production. In vegetative medium, the nanR null mutants produced more spores. These results suggest that NanR plays a crucial role in the regulation of c-cpe strains.
Article
Immunology
Jihong Li, Arhat Pradhan, Bruce A. McClane
Summary: Clostridium perfringens type F strains cause food poisoning by producing C. perfringens enterotoxin (CPE) during sporulation in the intestines. Most type F strains carry a chromosomal cpe gene (c-cpe strains). C. perfringens produces multiple sialidases, but some c-cpe strains only carry nanJ and nanH genes.
INFECTION AND IMMUNITY
(2023)
Article
Multidisciplinary Sciences
Alex J. Vecchio, Sewwandi S. Rathnayake, Robert M. Stroud
Summary: The study reveals the molecular and structural basis of how CpE targets susceptible claudins, as well as differences in the primary CpE receptors in mice and humans due to sequence changes in the target motif.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Microbiology
Jihong Li, A. Bruce McClane
Summary: Clostridium perfringens type F strains, common causes of food poisoning, consistently carry the nanH sialidase gene, which is associated with NanH production during sporulation. The presence of NanH enhances the cytotoxicity of CPE for cells, potentially serving as an accessory virulence factor for type F food poisoning.
Article
Medicine, Research & Experimental
Yinghua Ma, David Sannino, Jennifer R. Linden, Sylvia Haigh, Baohua Zhao, John B. Grigg, Paul Zumbo, Friederike Duendar, Daniel Butler, Caterina P. Profaci, Kiel Telesford, Paige N. Winokur, Kareem R. Rumah, Susan A. Gauthier, Vincent A. Fischetti, Bruce A. McClane, Francisco A. Uzal, Lily Zexter, Michael Mazzucco, Richard Rudick, David Danko, Evan Balmuth, Nancy Nealon, Jai Perumal, Ulrike Kaunzner, Ilana L. Brito, Zhengming Chen, Jenny Z. Xiang, Doron Betel, Richard Daneman, Gregory F. Sonnenberg, Christopher E. Mason, Timothy Vartanian
Summary: Multiple sclerosis (MS), a complex disease of the CNS, has been linked to gut dysbiosis. This study found that individuals with MS were more likely to have epsilon toxin-producing strains of C. perfringens in their gut microbiomes compared to healthy controls. These strains were able to produce functional epsilon toxin and had genetic characteristics similar to highly conjugative plasmids. In an animal model of MS, epsilon toxin could substitute for pertussis toxin and induced demyelination in various regions of the CNS, similar to lesions seen in MS patients.
JOURNAL OF CLINICAL INVESTIGATION
(2023)
Article
Chemistry, Multidisciplinary
Man Kyu Shim, Jinhee Na, In Kyung Cho, Eun Hyang Jang, Jooho Park, Sangmin Lee, Jong-Ho Kim
Summary: The study developed a claudin-4-targeting drug carrier that efficiently accumulated in pancreatic cancer tissues, suppressed tumor growth with minimal toxicity to normal tissues. This claudin-4-targeting drug carrier may represent a promising new approach for treating pancreatic cancer.
JOURNAL OF CONTROLLED RELEASE
(2021)
Article
Microbiology
Mauricio A. Navarro, Jihong Li, Juliann Beingesser, Bruce A. McClane, Francisco A. Uzal
Summary: NanI can significantly enhance the activity of C. perfringens enterotoxin (CPE) in the presence of mucus, leading to increased cytotoxicity and damage to intestinal mucosa.
Review
Oncology
Amita R. Banga, Peace Odiase, Kartik Rachakonda, Amar P. Garg, Samuel E. Adunyah, Girish Rachakonda
Summary: This article discusses the potential value of the interaction between Clostridium perfringens Enterotoxin (CPE) and Claudin-4 in the treatment of breast cancer metastasis to the brain, and introduces the important role of Claudin-4 in cell trafficking and disease development.
Article
Microbiology
Ke Wu, Zhe Li, Mingjin Fang, Yuan Yuan, Edward M. Fox, Yingqiu Liu, Ruichao Li, Li Bai, Wen Zhang, Wei-Min Zhang, Qi Yang, Lingling Chang, Pu Li, Xinglong Wang, Juan Wang, Zengqi Yang
Summary: Antimicrobial resistance is a global concern threatening food safety and public health. A novel plasmid type called optrA can harbor multiple antibiotic resistance genes, potentially acting as a reservoir for antibiotic resistance genes in Clostridium perfringens. This plasmid type can also coexist with pCW3-like plasmids and pCP13-like plasmids encoding toxin genes associated with gastrointestinal diseases, posing a potential threat to public health.
Review
Agriculture, Dairy & Animal Science
Francisco A. Uzal, Federico Giannitti, Javier Asin
Summary: Yellow lamb disease is a poorly understood condition in young sheep caused by certain strains of Clostridium perfringens type A. It is characterized by hemolysis, jaundice, and anemia. Diagnosis of the disease is challenging due to the presence of the microorganism in the intestines of healthy sheep. There is currently no specific vaccine for yellow lamb disease.
Article
Biochemistry & Molecular Biology
Panpan Lin, Rongbang Tan, Ping Yu, Yanyu Li, Yuqian Mo, Wen Li, Jingjing Zhang
Summary: The blood-brain barrier is essential for protecting the central nervous system, but it also hinders efficient drug delivery. This study demonstrates that a mutated fragment of Clostridium perfringens enterotoxin can regulate the dynamics of the endothelial tight junction protein claudin-5 and influence blood-brain barrier permeability.
Article
Food Science & Technology
Thea Neumann, Maren Krueger, Jasmin Weisemann, Stefan Mahrhold, Daniel Stern, Martin B. Dorner, Cecile Feraudet-Tarisse, Christopher Poehlmann, Katharina Schulz, Ute Messelhaeusser, Dagmar Rimek, Frank Gessler, Thomas Elssner, Stephanie Simon, Andreas Rummel, Brigitte G. Dorner
Summary: The study successfully identified nine specific mAbs targeting CPE and showed their high affinity in detecting CPE. Through ELISAs, CPE can be detected specifically, which is useful for diagnosing diarrhea in clinical laboratories.
Article
Oncology
Jessica Pahle, Dennis Kobelt, Jutta Aumann, Diana Behrens, Ole Daberkow, Margarita Mokritzkij, Jorg Piontek, Ulrike Stein, Wolfgang Walther
Summary: A novel suicide gene therapy treatment based on Clostridium perfringens enterotoxin (CPE) has been developed to target claudin-3 and -4 (Cldn3/4) overexpressing pancreatic cancer cells, leading to rapid eradication and apoptotic/necrotic signaling. In vitro and in vivo studies demonstrate the selective toxicity and efficacy of this approach in treating pancreas carcinomas, with translational potential for clinical applications in combination with conventional chemotherapy.
Article
Microbiology
Marc Liggins, Norma Ramirez Ramirez, Ernesto Abel-Santos
Summary: Clostridium perfringens is an anaerobic, Gram-positive bacterium that forms spores to survive in harsh environments. It can germinate through two distinct pathways, the amino acid-only pathway and the bile salt/amino acid pathway. Different strains of C. perfringens have strain-specific spore formation requirements, but the germination responses are consistent among all strains.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Kengo Ueda, Kazuki Kawahara, Narumi Kimoto, Yusuke Yamaguchi, Kazuhiro Yamada, Hiroya Oki, Takuya Yoshida, Shigeaki Matsuda, Yuki Matsumoto, Daisuke Motooka, Kentaro Kawatsu, Tetsuya Iida, Shota Nakamura, Tadayasu Ohkubo, Shinya Yonogi
Summary: This study characterized the genomic epidemiology of bec-harbouring C. perfringens strains and highlighted the importance of pCP13-like plasmids for the dissemination of pathogenic strains. The plasmids were found to have a high gene transfer rate and the ability to integrate various foreign genes, including those associated with virulence.