Article
Biochemistry & Molecular Biology
Jorge Soria-Bustos, Zeus Saldana-Ahuactzi, Partha Samadder, Jorge A. Yanez-Santos, Ygnacio Martinez Laguna, Maria L. Cedillo-Ramirez, Jorge A. Giron
Summary: This study investigates the relationship between flagella expression and the type III secretion system (T3SS) in enteropathogenic Escherichia coli (EPEC). The results suggest that the assembly of an intact T3SS is required for flagella production, and certain regulator genes play a role in activating or inhibiting flagella expression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Fabiola Avelino-Flores, Jorge Soria-Bustos, Zeus Saldana-Ahuactzi, Ygnacio Martinez-Laguna, Jorge A. Yanez-Santos, Maria L. Cedillo-Ramirez, Jorge A. Giron
Summary: The study investigated the influence of various environmental and nutritional signals on the expression of the flagellin gene in EPEC, finding that specific conditions can significantly enhance fliC transcription. When EPEC encounters certain signals in the intestinal niche, it may activate fliC expression to promote growth and colonization in the host.
Article
Ecology
Ayush Pathak, Daniel C. Angst, Ricardo Leon-Sampedro, Alex R. Hall
Summary: Some bacterial resistance mechanisms can degrade antibiotics, potentially protecting neighboring susceptible cells. However, the effects of such mechanisms on bacterial communities of more than two species are not well understood. By conducting experiments on multispecies communities, we found that resistance in one species reduced antibiotic inhibition of other species, but the extent of benefit varied among species.
Article
Microbiology
Jun Bong Lee, Se Kye Kim, Dalmuri Han, Jang Won Yoon
Summary: For the first time, it is shown that disrupting both relA and spoT genes in enteropathogenic Escherichia coli E2348/69 can attenuate its virulence and induce interleukin 6 (IL-6) in vivo. Whole genome-scale transcriptomic analysis revealed differential expression of 1,564 EPEC genes in the Delta relA Delta spoT double mutant strain. Depletion of relA and spoT attenuated the virulence of E2348/69 and induced IL-6 in porcine macrophages.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Entomology
Robin Y. Chen, B. Andrew Keddie
Summary: The use of Galleria mellonella as a model for studying enteropathogenic Escherichia coli demonstrated disease symptoms such as increased mortality and delayed pupation, which were dose-dependent. The Ill secretion system in EPEC was only partially responsible for virulence in G. mellonella, with most of the virulence mechanisms remaining unknown. Future research using this system will focus on understanding EPEC virulence and potential immune responses.
JOURNAL OF INSECT SCIENCE
(2021)
Article
Veterinary Sciences
Rie Ikeda, Fanny Laforet, Celine Antoine, Mare Adachi, Keiji Nakamura, Audrey Habets, Cassandra Kler, Klara De Rauw, Tetsuya Hayashi, Jacques G. Mainil, Damien Thiry
Summary: This study compared the virulence of different strains of STEC, EPEC, and E. coli in G. mellonella larvae, and found that STEC and EPEC O80:H2, as well as some E. coli O80:non-H2, were highly lethal to the larvae. The virulence factors of E. coli O80:non-H2 were not identified. However, G. mellonella larvae have limitations in determining the actual virulence properties of bacterial pathogens.
VETERINARY SCIENCES
(2023)
Article
Microbiology
Peter W. S. Hill, Ana Laura Moldoveanu, Molly Sargen, Severin Ronneau, Izabela Glegola-Madejska, Catrin Beetham, Robert A. Fisher, Sophie Helaine
Summary: Tolerance and persistence are superficially similar survival mechanisms for bacteria under antibiotic pressure, but they are underpinned by different physiological processes. Multidrug-tolerant bacteria enter a near-dormant state to avoid immune-mediated genotoxic damage, while persisters remain active to withstand macrophage-induced DNA breaks.
CELL HOST & MICROBE
(2021)
Article
Biology
Irina A. Rodionova, Ye Gao, Anand Sastry, Ying Hefner, Hyun Gyu Lim, Dmitry A. Rodionov, Milton H. Saier, Bernhard O. Palsson
Summary: The study reveals that the putative transporter gene, ydhC, and its regulator, ydhB, are involved in purine transportation, with YdhB activating the expression of the ydhC gene in E. coli. The authors propose renaming the regulator PunR and the transporter PunC.
COMMUNICATIONS BIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Sulaiman F. Aljasir, Dennis J. D'Amico
Summary: This study demonstrates the potential of Hafnia alvei B16 to attenuate Salmonella virulence and protect against pathogen invasion of intestinal epithelial cells, thereby enhancing food safety.
Article
Gastroenterology & Hepatology
Maximilian Baumgartner, Rebecca Zirnbauer, Sabine Schlager, Daniel Mertens, Nikolaus Gasche, Barbara Sladek, Craig Herbold, Olga Bochkareva, Vera Emelianenko, Harald Vogelsang, Michaela Lang, Anton Klotz, Birgit Moik, Athanasios Makristathis, David Berry, Stefanie Dabsch, Vineeta Khare, Christoph Gasche
Summary: With increasing urbanization and industrialization, the prevalence of inflammatory bowel diseases (IBDs) has been rising over the past two decades. This study found that the emerging pathogen atypical enteropathogenic E. coli (aEPEC) is associated with disease activity in ulcerative colitis (UC) patients, and it may disrupt immune-microbiota homeostasis.
Article
Gastroenterology & Hepatology
Jennifer Lising Roxas, Shylaja Ramamurthy, Katie Cocchi, Ilga Rutins, Anusha Harishankar, Al Agellon, John Scott Wilbur, Gresa Sylejmani, Gayatri Vedantam, V. K. Viswanathan
Summary: Bacterial pathogens manipulate host cell structures and functions, contributing to disease symptoms. Enteropathogenic Escherichia coli (EPEC) secretes proteins that alter host cell behavior. Two secreted proteins, EspZ and EspH, have contrasting effects on host mitochondria. EspZ interacts with FIS1 and protects the host mitochondrial network, enhancing host cell viability. In contrast, EspH increases FIS1 and leads to significant mitochondrial fragmentation and host cell death, facilitating pathogen dispersal.
Article
Food Science & Technology
Peter Kotsoana Montso, Cornelius Carlos Bezuidenhout, Charlotte Mienie, Yinka M. Somorin, Olumide A. Odeyemi, Victor Mlambo, Collins Njie Ateba
Summary: This study investigated the genetic diversity, virulence, and antimicrobial resistance of atypical enteropathogenic E. coli O177 strains isolated from cattle feces in South Africa. The findings showed that cattle carry genetically diverse E. coli O177 strains with a repertoire of virulence and resistance genes, highlighting the need for surveillance of multidrug resistant E. coli O177 strains in cattle.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Xi Lu, Dingyan Wu, Xin Zhao, Mingxin Zhang, Ke Ren, Ningning Zhou, Yanni Zhao, Weisheng Qian
Summary: Enterotoxigenic Escherichia coli (ETEC), a diarrheal pathogen, can utilize ethanolamine (EA) as a nitrogen source, which enhances its virulence phenotype and causes damage to host intestinal cells. The concentration of EA in the intestine also impacts the metabolic profile and pathogenicity of ETEC.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Microbiology
Ashok R. Nayak, Pradip K. Singh, Jinlei Zhao, Montserrat Samso, Michael S. Donnenberg
Summary: Type 4 pili are important surface appendages on bacteria and archaea that can extend and retract with great force. This study presents the detailed structure of an enzyme that converts chemical energy to mechanical energy in a human pathogen, revealing a mechanism of action not previously seen in enzymes that power type 4 pilus extension.
Article
Multidisciplinary Sciences
Gili Rosenberg, Dror Yehezkel, Dotan Hoffman, Camilla Ciolli Mattioli, Moran Fremder, Hadar Ben-Arosh, Leia Vainman, Noa Nissani, Shelly Hen-Avivi, Shirley Brenner, Maxim Itkin, Sergey Malitsky, Ehud Ohana, Noa Bossel Ben-Moshe, Roi Avraham
Summary: The translation discusses the importance of intracellular pathogens sensing and responding to changing host cell environments, and how Salmonella Typhimurium utilizes succinate accumulation in macrophages to enhance its antimicrobial resistance and virulence.
Meeting Abstract
Biochemistry & Molecular Biology
Michael McKinley, Kaylani Almeida, Sammy Bedoui, David Farmer, Dianna Hocking, Yugeesh Lankadeva, Davide Martelli, Clive May, Roy Robins-Browne, Robin McAllen
Article
Immunology
Nicole L. Messina, Deborah A. Williamson, Roy Robins-Browne, Penelope A. Bryant, Nigel Curtis
PEDIATRIC INFECTIOUS DISEASE JOURNAL
(2020)
Letter
Dentistry, Oral Surgery & Medicine
A. Riad, B. Hockova
BRITISH DENTAL JOURNAL
(2020)
Article
Pathology
Eun Young Kang, Dane Cheasley, Cecile LePage, Matthew J. Wakefield, Michelle da Cunha Torres, Simone Rowley, Carolina Salazar, Zhongyue Xing, Prue Allan, David D. L. Bowtell, Anne-Marie Mes-Masson, Diane M. Provencher, Kurosh Rahimi, Linda E. Kelemen, Peter A. Fasching, Jennifer A. Doherty, Marc T. Goodman, Ellen L. Goode, Suha Deen, Paul D. P. Pharoah, James D. Brenton, Weiva Sieh, Constantina Mateoiu, Karin Sundfeldt, Linda S. Cook, Nhu D. Le, Michael S. Anglesio, C. Blake Gilks, David G. Huntsman, Catherine J. Kennedy, Nadia Traficante, Anna DeFazio, Scott Kaufmann, Michael Churchman, Charlie Gourley, Andrew N. Stephens, Nicola S. Meagher, Susan J. Ramus, Yoland C. Antill, Ian Campbell, Clare L. Scott, Martin Kobel, Kylie L. Gorringe
Summary: The study confirmed the ability of TP53 IHC to predict TP53 mutation status in ovarian mucinous tumors and showed that TP53 mutation is associated with a higher risk of mortality in MBOT patients.
Letter
Cell Biology
Felix K. F. Kommoss, Dane Cheasley, Matthew J. Wakefield, Clare L. Scott, Ian G. Campbell, C. Blake Gilks, Kylie Gorringe
Article
Immunology
Laure F. Pittet, Marc Tebruegge, Binita Dutta, Susan Donath, Nicole Messina, Dan Casalaz, Willem A. Hanekom, Warwick J. Britton, Roy Robins-Browne, Nigel Curtis, Nicole Ritz
Summary: BCG vaccination induces M. ulcerans-specific immune responses in infants, likely explaining the cross-protective effect observed in epidemiological studies. These immune responses show similar patterns to those observed in M. tuberculosis-stimulated samples.
Article
Cell Biology
Christoph Grohmann, Francesca Walker, Mark Devlin, Meng-Xiao Luo, Anderly C. Chueh, Judy Doherty, Francois Vaillant, Gwo-Yaw Ho, Matthew J. Wakefield, Clare E. Weeden, Alvin Kamili, Jayne Murray, Sela T. Po'uha, Janet Weinstock, Serena R. Kane, Maree C. Faux, Esmee Broekhuizen, Ye Zheng, Kristy Shield-Artin, Nadia J. Kershaw, Chin Wee Tan, Helen M. Witchard, Gregor Ebert, Susan A. Charman, Ian Street, Maria Kavallaris, Michelle Haber, Jamie I. Fletcher, Marie-Liesse Asselin-Labat, Clare L. Scott, Jane E. Visvader, Geoffrey J. Lindeman, Keith G. Watson, Antony W. Burgess, Guillaume Lessene
Summary: Targeting cell division by the new small molecule WEHI-7326 shows promising anti-tumor activity with broad-spectrum efficacy, providing a potential new therapeutic option for cancer patients.
CELL DEATH & DISEASE
(2021)
Article
Oncology
Ksenija Nesic, Olga Kondrashova, Rachel M. Hurley, Cordelia D. McGehee, Cassandra J. Vandenberg, Gwo-Yaw Ho, Elizabeth Lieschke, Genevieve Dall, Nirashaa Bound, Kristy Shield-Artin, Marc Radke, Ashan Musafer, Zi Qing Chai, Mohammad Reza Eftekhariyan Ghamsari, Maria Harrell, Damien Kee, Inger Olesen, Orla McNally, Nadia Traficante, Anna DeFazio, David D. L. Bowtell, Elizabeth M. Swisher, S. John Weroha, Katia Nones, Nicola Waddell, Scott H. Kaufmann, Alexander Dobrovic, Matthew J. Wakefield, Clare L. Scott
Summary: This study revealed that methylation patterns in the RAD51C promoter are closely associated with sensitivity to PARP inhibitors, with complete or heterogeneous methylation affecting drug resistance development. Additionally, genomic profiling showed that resistance to PARPi can be acquired independently in genetically distinct lineages.
Article
Critical Care Medicine
Laure F. Pittet, Nora Fritschi, Marc Tebruegge, Binita Dutta, Susan Donath, Nicole L. Messina, Dan Casalaz, Willem A. Hanekom, Warwick J. Britton, Roy Robins-Browne, Nigel Curtis, Nicole Ritz
Summary: The presence of a scar after BCG vaccination is associated with stronger mycobacteria-specific T-cell responses. Further study on the relationship between scar formation and protection against tuberculosis as well as the need for revaccination in scar-negative individuals is important.
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2022)
Article
Oncology
Gwo Yaw Ho, Elizabeth L. Kyran, Justin Bedo, Matthew J. Wakefield, Darren P. Ennis, Hasan B. Mirza, Cassandra J. Vandenberg, Elizabeth Lieschke, Andrew Farrell, Anthony Hadla, Ratana Lim, Genevieve Dall, James E. Vince, Ngee Kiat Chua, Olga Kondrashova, Rosanna Upstill-Goddard, Ulla-Maja Bailey, Suzanne Dowson, Patricia Roxburgh, Rosalind M. Glasspool, Gareth Bryson, Andrew V. Biankin, Susanna L. Cooke, Gayanie Ratnayake, Orla McNally, Nadia Traficante, Anna DeFazio, S. John Weroha, David D. Bowtell, Iain A. McNeish, Anthony T. Papenfuss, Clare L. Scott, Holly E. Barker
Summary: Genomic analyses and preclinical models of ovarian carcinosarcoma support the conversion theory for disease development and indicate that microtubule inhibitors could be used to suppress EMT and stimulate antitumor immunity.
Article
Oncology
Nicola S. Meagher, Kylie L. Gorringe, Matthew Wakefield, Adelyn Bolithon, Chi Nam Ignatius Pang, Derek S. Chiu, Michael S. Anglesio, Kylie-Ann Mallitt, Jennifer A. Doherty, Holly R. Harris, Joellen M. Schildkraut, Andrew Berchuck, Kara L. Cushing-Haugen, Ksenia Chezar, Angela Chou, Adeline Tan, Jennifer Alsop, Ellen Barlow, Matthias W. Beckmann, Jessica Boros, David D. L. Bowtell, Alison H. Brand, James D. Brenton, Ian Campbell, Dane Cheasley, Joshua Cohen, Cezary Cybulski, Esther Elishaev, Ramona Erber, Rhonda Farrell, Anna Fischer, Zhuxuan Fu, Blake Gilks, Anthony J. Gill, Charlie Gourley, Marcel Grube, Paul R. Harnett, Arndt Hartmann, Anusha Hettiaratchi, Claus K. Hogdall, Tomasz Huzarski, Anna Jakubowska, Mercedes Jimenez-Linan, Catherine J. Kennedy, Byoung-Gie Kim, Jae-Weon Kim, Jae-Hoon Kim, Kayla Klett, Jennifer M. Koziak, Tiffany Lai, Angela Laslavic, Jenny Lester, Yee Leung, Na Li, Winston Liauw, Belle W. X. Lim, Anna Linder, Jan Lubinski, Sakshi Mahale, Constantina Mateoiu, Simone McInerny, Janusz Menkiszak, Parham Minoo, Suzana Mittelstadt, David Morris, Sandra Orsulic, Sang-Yoon Park, Celeste Leigh Pearce, John Pearson, Malcolm C. Pike, Carmel M. Quinn, Ganendra Raj Mohan, Jianyu Rao, Marjorie J. Riggan, Matthias Ruebner, Stuart Salfinger, Clare L. Scott, Mitul Shah, Helen Steed, Colin J. R. Stewart, Deepak Subramanian, Soseul Sung, Katrina Tang, Paul Timpson, Robyn L. Ward, Rebekka Wiedenhoefer, Heather Thorne, Paul A. Cohen, Philip Crowe, Peter A. Fasching, Jacek Gronwald, Nicholas J. Hawkins, Estrid Hogdall, David G. Huntsman, Paul A. James, Beth Y. Karlan, Linda E. Kelemen, Stefan Kommoss, Gottfried E. Konecny, Francesmary Modugno, Sue K. Park, Annette Staebler, Karin Sundfeldt, Anna H. Wu, Aline Talhouk, Paul D. P. Pharoah, Lyndal Anderson, Anna DeFazio, Martin Kobel, Michael L. Friedlander, Susan J. Ramus
Summary: Clinical and gene expression data were analyzed to identify prognostic and diagnostic features of advanced-stage mucinous ovarian carcinoma (MOC) and differentiate it from gastrointestinal (GI) metastases. An infiltrative growth pattern was associated with poor prognosis, and high expression of THBS2 and TAGLN was linked to adverse prognosis in MOC. HER2 amplification or high mRNA expression was detected in some MOC cases, suggesting the potential of HER2-targeted therapy. MOC samples clustered with upper GI tumors, indicating similar biology and treatment strategies.
CLINICAL CANCER RESEARCH
(2022)
Article
Multidisciplinary Sciences
Zoe R. C. Marks, Nicole Campbell, Niamh Mangan, Cassandra S. Vandenberg, Linden S. Gearing, Antony Matthews, Jodee L. Gould, Michelle N. Tate, Georgie Wray-McCann, Le J. Ying, Sarah Rosli, Natasha Brockwell, Belinda Parker, San Lim, Maree Bilandzic, Elizabeth Christie, Andrew Stephens, Eveline de Geus, Matthew Wakefield, Gwo-Yaw Ho, Orla A. McNally, David D. L. Australian Ovarian Cancer Study, Iain A. McNeish, David D. L. L. Bowtell, Nicole M. de Weerd, Clare J. Scott, Nollaig Bourke, Paul Hertzog
Summary: High-grade serous ovarian cancers have low survival rates due to late presentation and chemoresistance. In this study, we investigate the tumor-suppressive activities of interferon-e (IFNe) in ovarian cancer models. We find that IFNe is constitutively expressed in the fallopian tube and lost during tumor development. IFNe directly acts on tumor cells and activates anti-tumor immunity. It prevents the accumulation and activation of suppressor cells and regulatory T cells. Our findings suggest that IFNe could be a potential therapeutic target for ovarian cancer.
Review
Oncology
Ana C. Veneziani, Clare Scott, Matthew J. Wakefield, Anna V. Tinker, Stephanie Lheureux
Summary: Poly (ADP-ribose) polymerase inhibitors (PARPis) have revolutionized the treatment of epithelial ovarian cancer, but resistance to PARPi has become a major concern. Ongoing studies aim to understand the mechanisms of resistance and explore potential therapeutic strategies to overcome this issue.
THERAPEUTIC ADVANCES IN MEDICAL ONCOLOGY
(2023)
Article
Endocrinology & Metabolism
A. Salpietro, V Sinatra, V Mottola, A. Ceravolo, V Chirico, L. Colavita, G. Ceravolo, E. Gitto, C. Cuppari, R. Chimenz
Summary: HUS is a thrombotic microangiopathy characterized by thrombocytopenia, non-immune microangiopathic hemolytic anemia, and acute kidney injury. It can be classified into typical and atypical types, with common pathogenetic features including damage of endothelial cells, intravascular hemolysis, coagulation abnormalities, and microthrombus formation.
JOURNAL OF BIOLOGICAL REGULATORS AND HOMEOSTATIC AGENTS
(2022)
Article
Biochemistry & Molecular Biology
Rachel M. Hurley, Cordelia D. McGehee, Ksenija Nesic, Cristina Correia, Taylor M. Weiskittel, Rebecca L. Kelly, Annapoorna Venkatachalam, Xiaonan Hou, Nicholas M. Pathoulas, X. Wei Meng, Olga Kondrashova, Marc R. Radke, Paula A. Schneider, Karen S. Flatten, Kevin L. Peterson, Marc A. Becker, Ee Ming Wong, Melissa S. Southey, Alexander Dobrovic, Kevin K. Lin, Thomas C. Harding, Iain McNeish, Christian A. Ross, Jill M. Wagner, Matthew J. Wakefield, Clare L. Scott, Paul Haluska, Andrea E. Wahner Hendrickson, Larry M. Karnitz, Elizabeth M. Swisher, Hu Li, S. John Weroha, Scott H. Kaufmann
Summary: This study demonstrates that PARPi resistance can reverse RAD51C methylation and restore RAD51C expression, providing a model for investigating the relationship between PARPi and platinum sensitivity.
